Primary production of protein: I. Comparison of net cellular carbon
... Barlow 1982, Li & Harrison 1982, Li & Platt 1982, Priscu & Goldman 1983). However, the interpretation of results from such studies have been complicated because not enough is known about physiological and biochemical factors which may influence observed patterns of incorporation in the field and the ...
... Barlow 1982, Li & Harrison 1982, Li & Platt 1982, Priscu & Goldman 1983). However, the interpretation of results from such studies have been complicated because not enough is known about physiological and biochemical factors which may influence observed patterns of incorporation in the field and the ...
Statistical analysis of DNA microarray data
... • The homologous superfamilies cluster proteins with highly similar structures and functions. The assignments of structures to fold groups and homologous superfamilies are made by sequence and structure comparisons. ...
... • The homologous superfamilies cluster proteins with highly similar structures and functions. The assignments of structures to fold groups and homologous superfamilies are made by sequence and structure comparisons. ...
Anaerobic protists and hidden mitochondria
... (Müller, 2000). The report of mitochondrial-type hsp70 genes in two microsporidians strongly suggests that this group of amitochondriates has also undergone secondary mitochondrial loss (Arisue et al., 2002). Polyamines are found in diverse cell types but the precise mechanism of polyamine metaboli ...
... (Müller, 2000). The report of mitochondrial-type hsp70 genes in two microsporidians strongly suggests that this group of amitochondriates has also undergone secondary mitochondrial loss (Arisue et al., 2002). Polyamines are found in diverse cell types but the precise mechanism of polyamine metaboli ...
Bimolecular fluorescence complementation (BiFC)
... Besides the given specificity of BiFC signals resulting from protein interactions, this does not overcome the need of controls for a clear interpretation of the result and to exclude false positive interactions. Such controls are recommended by testing mutants of the interactors with deleted binding ...
... Besides the given specificity of BiFC signals resulting from protein interactions, this does not overcome the need of controls for a clear interpretation of the result and to exclude false positive interactions. Such controls are recommended by testing mutants of the interactors with deleted binding ...
APPLICATION OF PANCREATIC ENZYMES IN HYDROLYSIS OF
... 3 kDa (25.6%). In the hydrolysates with trypsin and elastase, in addition to the products with small molecular masses, larger peptides (9.5 kDa, 34 kDa, 37 kDa, and 78 kDa) were also observed. The more extensive hydrolysis of EWPP with chymotrypsin may be attributed to its broader specificity to cle ...
... 3 kDa (25.6%). In the hydrolysates with trypsin and elastase, in addition to the products with small molecular masses, larger peptides (9.5 kDa, 34 kDa, 37 kDa, and 78 kDa) were also observed. The more extensive hydrolysis of EWPP with chymotrypsin may be attributed to its broader specificity to cle ...
Importance of main-chain hydrophobic free energy to the stability of
... spectrum, containing proteins from moderately thermophilic organisms and their mesophilic homologues. Here we select one pair from each family. The three-dimensional structures of all these proteins have been taken from Protein Data Bank [28]. For a given protein, the PDB files contain coordinates f ...
... spectrum, containing proteins from moderately thermophilic organisms and their mesophilic homologues. Here we select one pair from each family. The three-dimensional structures of all these proteins have been taken from Protein Data Bank [28]. For a given protein, the PDB files contain coordinates f ...
How do bacterial cells ensure that metalloproteins get the correct
... incorporated into vitamin B12 in enzymes, whereas nickel is restricted to nine known enzymes, including urease, hydrogenase and some superoxide dismutases. Manganese is an obligatory cofactor in some other superoxide dismutases and has a special role in oxygen-evolving photosynthetic bacteria for th ...
... incorporated into vitamin B12 in enzymes, whereas nickel is restricted to nine known enzymes, including urease, hydrogenase and some superoxide dismutases. Manganese is an obligatory cofactor in some other superoxide dismutases and has a special role in oxygen-evolving photosynthetic bacteria for th ...
The dynamics of plant plasma membrane proteins
... Plasma membrane proteins are not static but exhibit remarkable mobility (Fig. 1). This involves lateral diffusion within the plasma membrane, which has been implicated as an important determinant for controlling protein-protein interactions and is suggested to influence rates of intracellular sortin ...
... Plasma membrane proteins are not static but exhibit remarkable mobility (Fig. 1). This involves lateral diffusion within the plasma membrane, which has been implicated as an important determinant for controlling protein-protein interactions and is suggested to influence rates of intracellular sortin ...
Localization of growth and secretion of proteins in
... positioned between the lower polycarbonate membrane and the agar medium. Glucoamylase was detected on the PVDF membrane using an antiserum raised against a purified glucoamylase from A . niger. Immunoreactions were done as described below for immunoblotting with goat-anti-rabbit-conjugated alkaline ...
... positioned between the lower polycarbonate membrane and the agar medium. Glucoamylase was detected on the PVDF membrane using an antiserum raised against a purified glucoamylase from A . niger. Immunoreactions were done as described below for immunoblotting with goat-anti-rabbit-conjugated alkaline ...
The proteomics of plant cell membranes
... membrane proteins will be discussed in the light of recent proteomics data, notably for the chloroplast envelope, mitochondrial membranes and the plasma membrane from Arabidopsis. Chloroplasts perform vital biosynthetic functions, and many processes are located exclusively within these unique organe ...
... membrane proteins will be discussed in the light of recent proteomics data, notably for the chloroplast envelope, mitochondrial membranes and the plasma membrane from Arabidopsis. Chloroplasts perform vital biosynthetic functions, and many processes are located exclusively within these unique organe ...
Green Fluorescent Protein
... GFP as a biosensor GFP is used within various fields of biology like molecular biology, neuroscience and cell biology. It can be used as a reporter gene, cell marker, fusion tag, and for quantitative monitoring of gene expression. GFP’s fluorescence characteristic can also be used in methods design ...
... GFP as a biosensor GFP is used within various fields of biology like molecular biology, neuroscience and cell biology. It can be used as a reporter gene, cell marker, fusion tag, and for quantitative monitoring of gene expression. GFP’s fluorescence characteristic can also be used in methods design ...
updated ppt slides - UCLA Chemistry and Biochemistry
... to approach the Kd of the enzyme-substrate complex. b. Briefly define ‘first-order’ as it applies to rate constants (15 words or less). c. Of the rate-constants above, which are first-order? d. Write two different expressions for the Kd of the enzyme substrate complex. e. Under what condition is ES ...
... to approach the Kd of the enzyme-substrate complex. b. Briefly define ‘first-order’ as it applies to rate constants (15 words or less). c. Of the rate-constants above, which are first-order? d. Write two different expressions for the Kd of the enzyme substrate complex. e. Under what condition is ES ...
FERMENTATION PROBLEMS: STUCK/SLUGGISH FERMENTATIONS
... • Survival factors deficiency: Sterols and Fatty acids improve yeast cell resistance to stress conditions and increases their viability. Difficulty in maintaining viable biomass is usually due to a deficiency of survival factors. See our Yeast Nutrition newsletter. • High alcohol content: Alcohol pa ...
... • Survival factors deficiency: Sterols and Fatty acids improve yeast cell resistance to stress conditions and increases their viability. Difficulty in maintaining viable biomass is usually due to a deficiency of survival factors. See our Yeast Nutrition newsletter. • High alcohol content: Alcohol pa ...
TD3 5.08 – PKS/NRPS pathways and mass
... - biosynthesizes yersiniabactin (Ybt), an Fe III chelator - Ybt strips Fe3+ from host proteins and is used for Y pestis survival - Ybt synthesized by hybrid NRPS/PKS system D= Dalton = g/mol ...
... - biosynthesizes yersiniabactin (Ybt), an Fe III chelator - Ybt strips Fe3+ from host proteins and is used for Y pestis survival - Ybt synthesized by hybrid NRPS/PKS system D= Dalton = g/mol ...
Indian Agricultural Research Institute, New Delhi
... Pearl millet TF 23A x D356 High P stocks 17.8% P, 2.55 % lys 21.7% P, 1.97 % lys (W or Y D 118 23.0% P, 1.98 % lys endosperm) Bichpuri local J.S Bhat, Coarse cereals presentation ...
... Pearl millet TF 23A x D356 High P stocks 17.8% P, 2.55 % lys 21.7% P, 1.97 % lys (W or Y D 118 23.0% P, 1.98 % lys endosperm) Bichpuri local J.S Bhat, Coarse cereals presentation ...
Changes in chemical composition in male turkeys
... than unity for the carcass and lower than unity for the viscera and feathers (Table 2). The allometric scalar for viscera was lower than that of feathers, indicating that the viscera content decreased more rapidly than the feather content. In general, the protein and ash contents in FFB declined wit ...
... than unity for the carcass and lower than unity for the viscera and feathers (Table 2). The allometric scalar for viscera was lower than that of feathers, indicating that the viscera content decreased more rapidly than the feather content. In general, the protein and ash contents in FFB declined wit ...
Expressway™ Cell-Free E. coli Expression System
... to allow high-level synthesis of your recombinant protein of interest. At one or several time points after initiating the protein synthesis reaction, the reaction is supplemented with an optimized Feed Buffer containing a proprietary mixture of salts, amino acids, and other substrates that are deple ...
... to allow high-level synthesis of your recombinant protein of interest. At one or several time points after initiating the protein synthesis reaction, the reaction is supplemented with an optimized Feed Buffer containing a proprietary mixture of salts, amino acids, and other substrates that are deple ...
slides - NMRbox
... Parameters and Mutations on the High Order Structure of Filgrastim by NMR. Pharm Res., 32, 3365-3375 (2015). ...
... Parameters and Mutations on the High Order Structure of Filgrastim by NMR. Pharm Res., 32, 3365-3375 (2015). ...
Membrane Vesicles Isolated from Sugar Beet Leaves
... port those amino acids to the heterotrophic tissues that are dependent upon imported carbon and nitrogen for normal growth and development. Amino acid transport in plants is frequently associated with accumulation against a significant free energy gradient across the plasma membrane. Several lines o ...
... port those amino acids to the heterotrophic tissues that are dependent upon imported carbon and nitrogen for normal growth and development. Amino acid transport in plants is frequently associated with accumulation against a significant free energy gradient across the plasma membrane. Several lines o ...
Incorporation of non-natural amino acids into proteins Takahiro
... an amber suppressor tRNATyr and a TyrRS from Methanococcus jannaschii has been mutated to become orthogonal to any aaRS/tRNA pair from E. coli [19,20]. The latter TyrRS was further mutated not to accept tyrosine or any other amino acids, but to accept O-methyl-tyrosine (44) exclusively as the substr ...
... an amber suppressor tRNATyr and a TyrRS from Methanococcus jannaschii has been mutated to become orthogonal to any aaRS/tRNA pair from E. coli [19,20]. The latter TyrRS was further mutated not to accept tyrosine or any other amino acids, but to accept O-methyl-tyrosine (44) exclusively as the substr ...
Signal Sequences Specify the Targeting Route to the Endoplasmic
... based on principles similar to those used previously (Manoil and Beckwith, 1985; Deshaies and Schekman, 1987). The strategy uses a chimeric reporter consisting of a normally cytosolic enzyme fused to a targeting domain that directs translocation of the enzyme into the E R lumen, where it is inactive ...
... based on principles similar to those used previously (Manoil and Beckwith, 1985; Deshaies and Schekman, 1987). The strategy uses a chimeric reporter consisting of a normally cytosolic enzyme fused to a targeting domain that directs translocation of the enzyme into the E R lumen, where it is inactive ...