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a server for analyzing and predicting RNA
a server for analyzing and predicting RNA

... for each residue; ‘þ’ for residues that are within the specified distance cutoff and ‘’ for residues that do not have any atoms within the distance cutoff. The calculated RNA-binding residues are also displayed on the PDB structure of the protein–RNA complex using Jmol (www.jmol.org). By default, the ...
The nucleolus and herpesviral usurpation
The nucleolus and herpesviral usurpation

... with either NLS2 or NLS3 are able to function as NoLSs, which target the ORF57 protein to the nucleolus. Disruption of the nucleolus by actinomycin D or 5,6dichloro-1-b-D-ribofuranosylbenzimidazole leads to a reduction of KSHV ORF57-mediated intronless mRNA export, revealing that the intact nucleolu ...
Two Domains of the Human bZIP Transcription Factor
Two Domains of the Human bZIP Transcription Factor

... of the antioxidant response. In addition, TCF11 has also been suggested to play a role in tumor necrosis factor-␣ regulation, because specific interaction with the tumor necrosis factor-␣ promoter has been demonstrated in the cell line DC18 (10). TCF11 is widely expressed (1) and has the potential o ...
Rhodopsin
Rhodopsin

... •Rhodopsin works best at dim light, responsible for night vision (too much light will saturate the protein) •Rod density is found greater on the outer edges of the retina (peripheral vision) ...
Protein 4.2 interaction with hereditary spherocytosis mutants of the
Protein 4.2 interaction with hereditary spherocytosis mutants of the

... predominant of two protein 4.2 splice variants. Type I is the less common larger isoform and has an additional 30 amino acids near the N-terminus [11]. Amino acid numbering of protein 4.2 in the present paper will be according to the Type II isoform. Protein 4.2 is a member of the TG (transglutamina ...
ESCRT requirements for EIAV budding | SpringerLink
ESCRT requirements for EIAV budding | SpringerLink

... Vps60p/CHMP5 [43,44] and Vps4p/VPS4A/B, and stimulates enzyme assembly and ATPase activity [45-48]; and the ESCRT-III adaptor protein, Bro1p/ALIX, which binds and stabilizes the Snf7p/CHMP4A/B/C filaments, and recruits the deubiquitinating enzyme, Doa4p/UBPY [23,49]. Although the core yeast ESCRT co ...
Lysines 72, 80 and 213 and aspartic acid 210 of the
Lysines 72, 80 and 213 and aspartic acid 210 of the

... MG5267, containing a single chromosomal copy of the lac operon (Van Rooijen et al., 1992), in which the lacR gene has been deleted by replacement recombination. This strain was used as an expression host for the mutated lacR genes. Plasmid pNZ3016 contains the lacR gene (Figure 1) and is based on th ...
Topology Prediction of Membrane Proteins
Topology Prediction of Membrane Proteins

... (http://www.rcsb.org/pdb). The fraction is hence less than 1% which should be compared to the observation that between 20 and 30% of all proteins are membrane proteins (Krogh et al., 2001; Wallin and von Heijne, 1998). The huge gap is not expected to be filled in the foreseeable future even though t ...
Electrophoretic Properties of Native Proteins
Electrophoretic Properties of Native Proteins

... Some proteins contain other chemical groups in addition to their amino acid residues. For example, hemoglobin and cytochrome c contain heme, which consists of iron complexed to a system of organic rings called porphyrin. “Non-amino acid” structural groups covalently attached or very tightly bound to ...
Finding Patterns in Protein Sequence and Structure
Finding Patterns in Protein Sequence and Structure

... complete gene is thus ~9 kb of exon and ~177 kb of intron. • The biggest human gene yet is for dystrophin. It has > 30 exons and is spread over 2.4 million bp. ...
proteins: three-dimensional structure
proteins: three-dimensional structure

... than the 3.6 residues per turn of a standard a helix, the two keratin helices are inclined about 18° relative to one another, resulting in the coiled coil arrangement. This conformation allows the contacting side chains to interdigitate (Fig. 6-14b). The higher order structure of a keratin is not we ...
Биохимия жидкостей полости рта
Биохимия жидкостей полости рта

... Cystatins perform antimicrobial and antiviral function by the inhibition of enzyme activity of cysteine proteases that hydrolyze proteins of the oral cavity. They inhibit the activity of cysteine proteases by specific binding in the active site of the enzyme with cysteine residues. ...
Lesson 4.Protein
Lesson 4.Protein

... valine, leucine, and isoleucine), as well as proline (with its unusual cyclic structure), methionine (one of the two sulfur-containing amino acids), and two aromatic amino acids, phenylalanine and tryptophan. Tryptophan is sometimes considered a borderline member of this group because it can interac ...
The lin-41 RBCC Gene Acts in the C. elegans Heterochronic
The lin-41 RBCC Gene Acts in the C. elegans Heterochronic

... while control animals did not (data not shown). In retarded animals, most hypodermal seam cells failed to execute the L/A switch (i.e., exit the cell cycle and terminally differentiate) at the L4 molt and instead reiterated the larval fate and divided again (Figures 2B–2E). In some cases, the animal ...
Determination of protein regions responsible for interactions of
Determination of protein regions responsible for interactions of

Recent advances in plant cell wall proteomics
Recent advances in plant cell wall proteomics

... Indeed, proteomic studies made on cell walls revealed leaderless proteins mixed with secreted proteins, many of which have well-known functions inside the cell. There have been many discussions about the possibility of finding non-canonical proteins in plant cell walls [7, 36]. Are they bona fide s ...
Environmentally Induced Changes in Amino Acid Composition in the
Environmentally Induced Changes in Amino Acid Composition in the

... human and animal diets. Environmental conditions are known to influence protein quantity as well as grain production and, in turn, amino acid composition. In this study, grain yield, protein content, and amino acid composition were determined in 10 durum wheat genotypes under three water and tempera ...
Selective Inhibition of Human Solute Carrier Transporters by
Selective Inhibition of Human Solute Carrier Transporters by

... ([3H]CCK-8 2 nM, 23.4 nCi); OAT1 ([3H]para-aminohippuric acid 5 mM, 144 nCi); OAT2 ([3H]E3S 2 mM, 138 nCi, pH 5.5); OAT4 ([3H]E3S 300 nM, 138 nCi); OCT1, OCT2, and OCT3 ([3H]MPP+ 100 nM, 98.5 nCi); OCTN1 ([3H]L-ergothioneine 5 mM, 40.8 nCi); and OCTN2 ([14C]L-carnitine 5 mM, 6.72 nCi). MKIs were add ...
Systematic comparison of single-chain Fv antibody
Systematic comparison of single-chain Fv antibody

... indicated times, as described in Methods. Red lines indicate the MFI obtained by staining Daudi cells with the scFv (continuous line) and mAb (dashed line) previously incubated at 37°C for the same time lengths as for the internalization experiment. MFI values are plotted as percentage relative to ...
Membrane biogenesis in anoxygenic photosynthetic prokaryotes
Membrane biogenesis in anoxygenic photosynthetic prokaryotes

... and structural analogs of BChl. Minimal requirements for reassociation include specific carbonyl and carbomethoxy groups of BChl, the Mg atom and the conserved core region of the apoprotein, as well as stabilizing interactions by ion-pairing in the N-terminal region. Clearly, conditions for in vitro ...
Pseudomonas aeruginosa B-band lipopolysaccharide genes wbpA
Pseudomonas aeruginosa B-band lipopolysaccharide genes wbpA

... functionally homologous to the highly conserved P. aeruginosa gene wbpM [16]. In the case of E. coli, as shown in Table 1, ECA also contains the amino sugar L-D-ManNAcA attached to L-D-GlcNAc. Therefore, all three sugar polymers contain singly or doubly acetylated mannosaminuronic acid residues. The ...
Cell-Free (In Vitro) Protein Expression
Cell-Free (In Vitro) Protein Expression

TFE3 contains two activation domains, one acidic and the other
TFE3 contains two activation domains, one acidic and the other

... is contained within the 102 amino acid C-terminal portion of TFE3 shown to be sufficient for activation (Fig. 1 A). Interestingly, although the proline content in the highly conserved region is low (3/38), all three prolines are perfectly conserved. The two TFE3 activation domains activate transcrip ...
Dietary protein for athletes - Inside Outside Wellness Center
Dietary protein for athletes - Inside Outside Wellness Center

... 19 y and older is 0.8 g proteinkg–1d–1. This Recommended Dietary Allowance (RDA) is cited as adequate for all persons. This amount of protein would be considered by many athletes as the amount to be consumed in a single meal, particularly for strength-training athletes. There does exist, however, ...
Noncovalently Associated Complexes: Apomyoglobin and
Noncovalently Associated Complexes: Apomyoglobin and

... was analyzed using the Agilent HPLC-Chip MS system interfaced to an Agilent 6210 TOF MS. Myoglobin is the resident oxygen-carrying molecule of cardiac and skeletal muscle. The myoglobin complex consists of the apomyoglobin protein and Heme b. The capacity of myoglobin to bind oxygen depends on the p ...
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Magnesium transporter

This page links directly from the magnesium in biological systems page.Magnesium transporters are proteins that transport magnesium across the cell membrane. All forms of life require magnesium, yet the molecular mechanisms of Mg2+ uptake from the environment and the distribution of this vital element within the organism are only slowly being elucidated.In bacteria, Mg2+ is probably mainly supplied by the CorA protein and, where the CorA protein is absent, by the MgtE protein. In yeast the initial uptake is via the Alr1p and Alr2p proteins, but at this stage the only internal Mg2+ distributing protein identified is Mrs2p. Within the protozoa only one Mg2+ transporter (XntAp) has been identified. In metazoa, Mrs2p and MgtE homologues have been identified, along with two novel Mg2+ transport systems TRPM6/TRPM7 and PCLN-1. Finally, in plants, a family of Mrs2p homologues has been identified along with another novel protein, AtMHX.
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