Chloroplast Division and Morphology Are Differentially Affected by
... whereas in plants with severe phenotypes (Fig. 3A, lanes 8–10), AtFtsZ1-1 protein levels were noticeably elevated. To further analyze this relationship, the level of AtFtsZ1-1 in the transgenic plants relative to that in control plants was quantified by immunoblotting (Fig. 4). For this purpose, a c ...
... whereas in plants with severe phenotypes (Fig. 3A, lanes 8–10), AtFtsZ1-1 protein levels were noticeably elevated. To further analyze this relationship, the level of AtFtsZ1-1 in the transgenic plants relative to that in control plants was quantified by immunoblotting (Fig. 4). For this purpose, a c ...
Protein splicing of PRP8 mini-inteins from species of the genus
... protein-splicing activity in E. coli cells. Plasmid-borne fusion genes were constructed to produce proteins in which the Penicillium inteins (plus their 5-aa native N-extein sequences and 4-aa native C-extein sequence) were fused to an N-terminal GST protein and a C-terminal His-tag (Fig. 3). Simila ...
... protein-splicing activity in E. coli cells. Plasmid-borne fusion genes were constructed to produce proteins in which the Penicillium inteins (plus their 5-aa native N-extein sequences and 4-aa native C-extein sequence) were fused to an N-terminal GST protein and a C-terminal His-tag (Fig. 3). Simila ...
Functional characterisation and cell walll interactions of
... 182, 196). S-layers are composed of regularly arranged protein subunits of a single protein or glycoprotein, which form a 2-dimensional layer that completely covers the cell (197). S-layer proteins can represent 10 to 15 % of the total protein content of a bacterial cell (23). The molecular masses o ...
... 182, 196). S-layers are composed of regularly arranged protein subunits of a single protein or glycoprotein, which form a 2-dimensional layer that completely covers the cell (197). S-layer proteins can represent 10 to 15 % of the total protein content of a bacterial cell (23). The molecular masses o ...
Auxin Action in a Cell
... Auxin Promotes the Interaction between the Aux/IAA Proteins and SCFTIR1 in Cell-Free Extracts Auxin treatment of Arabidopsis seedlings stimulates binding of Aux/IAA proteins by SCFTIR1 [2]. To further investigate this response, we tested the effects of auxin added directly to protein extracts prepar ...
... Auxin Promotes the Interaction between the Aux/IAA Proteins and SCFTIR1 in Cell-Free Extracts Auxin treatment of Arabidopsis seedlings stimulates binding of Aux/IAA proteins by SCFTIR1 [2]. To further investigate this response, we tested the effects of auxin added directly to protein extracts prepar ...
Word
... Mukherjee et al. 2011; Sang et al. 2011; Rueschhoff et al. 2013; Szydlowski et al. 2013), little is ...
... Mukherjee et al. 2011; Sang et al. 2011; Rueschhoff et al. 2013; Szydlowski et al. 2013), little is ...
Regulation cascade of flagellar expression in Gram‐negative bacteria
... [4], Escherichia coli [5], Vibrio cholerae [6], Salmonella typhimurium [7] and Helicobacter pylori [8]. Motility in pathogenic micro-organisms is usually considered a virulence factor, essential for colonisation of host organism or target organ [9,10]. However, the £agellar ¢lament bears strong anti ...
... [4], Escherichia coli [5], Vibrio cholerae [6], Salmonella typhimurium [7] and Helicobacter pylori [8]. Motility in pathogenic micro-organisms is usually considered a virulence factor, essential for colonisation of host organism or target organ [9,10]. However, the £agellar ¢lament bears strong anti ...
Effect of LIVNITRO on Protein Anabolism
... meal. Higher protein intakes when protein synthesis is maximized ...
... meal. Higher protein intakes when protein synthesis is maximized ...
Dehydrogenase in Saccharomyces cerevisiae
... at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predicted amino acid sequences and any known protein. Primary structure of yeast lipoamide dehydrogenase The primary structure, translated from the nucleotide sequence of the LPDl gene, contains ...
... at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predicted amino acid sequences and any known protein. Primary structure of yeast lipoamide dehydrogenase The primary structure, translated from the nucleotide sequence of the LPDl gene, contains ...
SAPS(1) USER COMMANDS SAPS(1) NAME saps
... protein sequence properties. Properties considered include compositional biases; clusters and runs of charge and other amino acid types; different kinds and extents of repetitive structures; locally periodic motifs; and anomalous spacings between identical residue types. The statistics are computed ...
... protein sequence properties. Properties considered include compositional biases; clusters and runs of charge and other amino acid types; different kinds and extents of repetitive structures; locally periodic motifs; and anomalous spacings between identical residue types. The statistics are computed ...
Dehydrogenase in Saccharomyces cerevisiae
... at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predicted amino acid sequences and any known protein. Primary structure of yeast lipoamide dehydrogenase The primary structure, translated from the nucleotide sequence of the LPDl gene, contains ...
... at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predicted amino acid sequences and any known protein. Primary structure of yeast lipoamide dehydrogenase The primary structure, translated from the nucleotide sequence of the LPDl gene, contains ...
structural basis for thermal stability of thermophilic trmd proteins
... misfolded proteins (Nagradova,N.K. 2004) In terms of protein stability under extreme conditions, the literature reveals a variety of structures found in proteins which might maintain stability at high temperatures. Interestingly, structures which might stabilize proteins under extreme conditions are ...
... misfolded proteins (Nagradova,N.K. 2004) In terms of protein stability under extreme conditions, the literature reveals a variety of structures found in proteins which might maintain stability at high temperatures. Interestingly, structures which might stabilize proteins under extreme conditions are ...
Nucleolar targeting of BN46/51 - Journal of Cell Science
... the differentiation demonstrated that BN46/51 is absent when basal bodies form or as the flagellar axoneme elongates, but BN46/51 appears when the cytoplasmic microtubule complex elongates from the basal body region. When flagellates spontaneously revert to amebae with the abrupt loss of the CMT, BN ...
... the differentiation demonstrated that BN46/51 is absent when basal bodies form or as the flagellar axoneme elongates, but BN46/51 appears when the cytoplasmic microtubule complex elongates from the basal body region. When flagellates spontaneously revert to amebae with the abrupt loss of the CMT, BN ...
Novel surface layer protein genes in Bacillus
... is enveloped by an S-layer with a square symmetry, which is composed of identical protein monomers (Raff, 2002). The cells of B. sphaericus JG-A12, like those of the closely related B. sphaericus NCTC 9602, are able to bind selectively and reversibly high amounts of metals such as uranium, lead, cop ...
... is enveloped by an S-layer with a square symmetry, which is composed of identical protein monomers (Raff, 2002). The cells of B. sphaericus JG-A12, like those of the closely related B. sphaericus NCTC 9602, are able to bind selectively and reversibly high amounts of metals such as uranium, lead, cop ...
Biochemistry Lecture 4 9/6/01
... • Basic structural units of proteins • All have 3 common functional grps: – -NH2, -COOH, -H ...
... • Basic structural units of proteins • All have 3 common functional grps: – -NH2, -COOH, -H ...
Creating Multiple Sequence Alignments
... The most basic task in sequence analysis is to ask whether two sequences are similar and can be compared. Proteins with very similar sequences probably share structural properties and similar functions. Objective: Explore different methods of sequence alignment, interpret their results, and compare ...
... The most basic task in sequence analysis is to ask whether two sequences are similar and can be compared. Proteins with very similar sequences probably share structural properties and similar functions. Objective: Explore different methods of sequence alignment, interpret their results, and compare ...
A Genomic Screen in Yeast Reveals Novel Aspects of
... proteasome. This finding not only suggested that the proteasome may degrade proteins encoded by nonstop mRNAs, but also supported previous observations that rapid decay of nonstop mRNAs cannot fully explain the lack of the encoded proteins. Further, we show that the proteasome and Ski7p affected exp ...
... proteasome. This finding not only suggested that the proteasome may degrade proteins encoded by nonstop mRNAs, but also supported previous observations that rapid decay of nonstop mRNAs cannot fully explain the lack of the encoded proteins. Further, we show that the proteasome and Ski7p affected exp ...
Cellular uptake of long-chain fatty acids: role of membrane
... The mechanism of FFA uptake by FABPpm is still not well established because it is a peripheral membrane protein; its role may be to capture unbound FFAs for transfer to other integral membrane fatty acid transporters (as described below) for fatty acid transport across the cell membranes. ...
... The mechanism of FFA uptake by FABPpm is still not well established because it is a peripheral membrane protein; its role may be to capture unbound FFAs for transfer to other integral membrane fatty acid transporters (as described below) for fatty acid transport across the cell membranes. ...
Mediation of Clathrin-Dependent Trafficking during
... of the GMC is disrupted when plants are grown above the permissive temperature range of 15 to 18°C, resulting in a missing or partial GC wall. While the cytokinesis-defective phenotype of scd1-1 predominantly manifests in GCs, cytokinesis of epidermal pavement cells is affected as well (Falbel et al ...
... of the GMC is disrupted when plants are grown above the permissive temperature range of 15 to 18°C, resulting in a missing or partial GC wall. While the cytokinesis-defective phenotype of scd1-1 predominantly manifests in GCs, cytokinesis of epidermal pavement cells is affected as well (Falbel et al ...
Protein Structure - Macmillan Learning
... To study the structure of a protein, the researcher must first purify it from all other proteins in the cell. First, cells are lysed and particulate matter is removed by centrifugation, to yield a “crude extract.” The crude extract is then fractionated to separate the proteins and isolate the one th ...
... To study the structure of a protein, the researcher must first purify it from all other proteins in the cell. First, cells are lysed and particulate matter is removed by centrifugation, to yield a “crude extract.” The crude extract is then fractionated to separate the proteins and isolate the one th ...
A Simple Method for Displaying the Hydropathic Character of a Protein
... however, nor is it clear that any solvent could ever meet that condition. This difficulty has been noted in the past, and it has been suggested that water-vapor transfer free energies would be a less complicated index of hydropathy (Hine & Mookerjee, 1975). The watervapor partition coefficients for ...
... however, nor is it clear that any solvent could ever meet that condition. This difficulty has been noted in the past, and it has been suggested that water-vapor transfer free energies would be a less complicated index of hydropathy (Hine & Mookerjee, 1975). The watervapor partition coefficients for ...
Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L
... Background: Sacha Inchi (Plukenetia volubilis L., Euphorbiaceae) is a potential oilseed crop because the seeds of this plant are rich in unsaturated fatty acids (FAs). In particular, the fatty acid composition of its seed oil differs markedly in containing large quantities of α-linolenic acid (18C:3 ...
... Background: Sacha Inchi (Plukenetia volubilis L., Euphorbiaceae) is a potential oilseed crop because the seeds of this plant are rich in unsaturated fatty acids (FAs). In particular, the fatty acid composition of its seed oil differs markedly in containing large quantities of α-linolenic acid (18C:3 ...
UNIT 2. Structure and function of proteins.
... gel are separated thanks to the solvent migration (buthanol: water: acetic acid 4:1:1) by capillarity. Reverse-phase high-performance liquid chromatography (HPLC): amino acids are separated on the base of their polarity by the used of a column having a ...
... gel are separated thanks to the solvent migration (buthanol: water: acetic acid 4:1:1) by capillarity. Reverse-phase high-performance liquid chromatography (HPLC): amino acids are separated on the base of their polarity by the used of a column having a ...
Coarse-Grained Modeling of ProteinDynamics
... A number of mean-resolution CG models have been developed for protein structure prediction (Kolinski and Skolnick 2004). Some of them enable efficient simulation of dynamic processes. A typical example is the CABS model (Kolinski 2004), which acronym stands for the united atoms representing a single ...
... A number of mean-resolution CG models have been developed for protein structure prediction (Kolinski and Skolnick 2004). Some of them enable efficient simulation of dynamic processes. A typical example is the CABS model (Kolinski 2004), which acronym stands for the united atoms representing a single ...
2.3. Three-Dimensional structure and function of proteins.
... Resins containing negatively charged groups interact with positive charge molecules, which elute from the resins by changing the pH buffer or the salt ion. Thin layer chromatography: amino acids absorbed on a thin layer of silica gel are separated thanks to the solvent migration (buthanol: water: ac ...
... Resins containing negatively charged groups interact with positive charge molecules, which elute from the resins by changing the pH buffer or the salt ion. Thin layer chromatography: amino acids absorbed on a thin layer of silica gel are separated thanks to the solvent migration (buthanol: water: ac ...