Bacterial Regulation
... (a) Lactose present, glucose scarce (cAMP level high): abundant lac mRNA synthesized. If glucose is scarce, the high level of cAMP activates CAP, and the lac operon produces large amounts of mRNA for the lactose pathway. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings ...
... (a) Lactose present, glucose scarce (cAMP level high): abundant lac mRNA synthesized. If glucose is scarce, the high level of cAMP activates CAP, and the lac operon produces large amounts of mRNA for the lactose pathway. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings ...
In-vitro Protein Production for Structure Determination with the Rapid
... Labs) with the RTS 500 E. coli HY Kit (Roche Molecular Biochemicals). A stock solution of 15N-labeled amino acids was prepared by dissolving 100 mg of the algal amino acid mixture in 6 ml of reconstitution buffer. Because the algal amino acid mixture is produced from acid hydrolysis of algal protein ...
... Labs) with the RTS 500 E. coli HY Kit (Roche Molecular Biochemicals). A stock solution of 15N-labeled amino acids was prepared by dissolving 100 mg of the algal amino acid mixture in 6 ml of reconstitution buffer. Because the algal amino acid mixture is produced from acid hydrolysis of algal protein ...
The Action of Chloramphenicol on Protein and Nucleic Acid
... necessary enzymes, which,presumably are protein in nature. Thus any specific inhibition of protein synthesis, in the absence of an effect on the activity of existing enzyme protein, might be expected t o produce a result similar to that reported in Fig. 2. That this effect was not observed by Wissem ...
... necessary enzymes, which,presumably are protein in nature. Thus any specific inhibition of protein synthesis, in the absence of an effect on the activity of existing enzyme protein, might be expected t o produce a result similar to that reported in Fig. 2. That this effect was not observed by Wissem ...
L-ASPG86 - Journal of Microbiology and Biotechnology
... animals [28], plants [42], algae [37], fungi [40], and bacteria [38], exists in two types: I and II. Type I asparaginase is a low-affinity enzyme found in the cytoplasm, whereas type II asparaginase is a high-affinity enzyme [36] identified within the periplasmic space [9]. L-Asparaginase was the fi ...
... animals [28], plants [42], algae [37], fungi [40], and bacteria [38], exists in two types: I and II. Type I asparaginase is a low-affinity enzyme found in the cytoplasm, whereas type II asparaginase is a high-affinity enzyme [36] identified within the periplasmic space [9]. L-Asparaginase was the fi ...
Chapter_7
... cleaved off from the protein during its life cycle and transport in the cell. They are therefore often removed from the database sequences before theoretical digestion – One can also take account of other information about the sequences for getting most reliable comparison, for example such as repor ...
... cleaved off from the protein during its life cycle and transport in the cell. They are therefore often removed from the database sequences before theoretical digestion – One can also take account of other information about the sequences for getting most reliable comparison, for example such as repor ...
Triphosphatase Related to the Protein Tyrosine Phosphatases
... Transcripts produced by RNA polymerase II are modified at their 59 end by the addition of a 7-methyl guanosine cap. Capping is the earliest modification of mRNA, occurring by the time the transcript is 25–30 nucleotides long (Jove and Manley, 1984; Rasmussen and Lis, 1993). The reaction occurs in th ...
... Transcripts produced by RNA polymerase II are modified at their 59 end by the addition of a 7-methyl guanosine cap. Capping is the earliest modification of mRNA, occurring by the time the transcript is 25–30 nucleotides long (Jove and Manley, 1984; Rasmussen and Lis, 1993). The reaction occurs in th ...
Alterations in oxidative phosphorylation complex proteins in the
... p38 might serve either protective or damaging roles, depending on conditions, such as the cellular and temporal context and differential activation of p38 isoforms. To begin to examine potential roles for p38 in the myocardium, we determined the effects of overexpressing an upstream activator of p38 ...
... p38 might serve either protective or damaging roles, depending on conditions, such as the cellular and temporal context and differential activation of p38 isoforms. To begin to examine potential roles for p38 in the myocardium, we determined the effects of overexpressing an upstream activator of p38 ...
PSLDoc: Protein subcellular localization prediction based on
... v.2.0, released in 2005, uses SVM as the underlying machine learning model and takes frequent subsequences occurring in proteins as input features. CELLO also uses SVM trained by multiple feature vectors derived from npeptide compositions. The updated CELLO II is based on a two-level SVM system: the ...
... v.2.0, released in 2005, uses SVM as the underlying machine learning model and takes frequent subsequences occurring in proteins as input features. CELLO also uses SVM trained by multiple feature vectors derived from npeptide compositions. The updated CELLO II is based on a two-level SVM system: the ...
Immunolocalization of maize transglutaminase and its substrates in
... The increase of TGase with chloroplast differentiation and its specific detection in the thylakoid appressed grana indicated that this enzyme might be related to the LHCII proteins of the antenna complex, which are localized in the same grana structures [12]. The 58 kDa band present in the lightexpo ...
... The increase of TGase with chloroplast differentiation and its specific detection in the thylakoid appressed grana indicated that this enzyme might be related to the LHCII proteins of the antenna complex, which are localized in the same grana structures [12]. The 58 kDa band present in the lightexpo ...
In vitro Nanoparticles Sareh Arjmand, Elham Bidram, Abbas
... interactions between the protein and polymer. This suggests that the NPs can be used to formulate and deliver the AAT protein as a drug. The main limitation of this process is that the protein is physically bound to the NPs’ surface and as a result, desorption of protein from the NPs’ surface can be ...
... interactions between the protein and polymer. This suggests that the NPs can be used to formulate and deliver the AAT protein as a drug. The main limitation of this process is that the protein is physically bound to the NPs’ surface and as a result, desorption of protein from the NPs’ surface can be ...
Biological ontologies for human functional annotation and
... “DNAs include DNA groups, families, molecules, domains, and regions” ...
... “DNAs include DNA groups, families, molecules, domains, and regions” ...
Design and application of stimulus
... Stimulus-responsive systems based on rational design Several peptide-based systems have rationally been engineered, both de novo and as modifications to existing proteins, to respond in interesting ways to various stimuli (Table I). Many of these systems are tunable via changes in temperature and/or ...
... Stimulus-responsive systems based on rational design Several peptide-based systems have rationally been engineered, both de novo and as modifications to existing proteins, to respond in interesting ways to various stimuli (Table I). Many of these systems are tunable via changes in temperature and/or ...
lec-04-transcript
... the opposite side of the peptide bond. This configuration is allowing less steric clashes whereas if you look at the bottom panel the cis configuration, there are two C-α on the same side of the peptide bond so there is more probability of having steric clashes. Therefore peptide bonds in the protei ...
... the opposite side of the peptide bond. This configuration is allowing less steric clashes whereas if you look at the bottom panel the cis configuration, there are two C-α on the same side of the peptide bond so there is more probability of having steric clashes. Therefore peptide bonds in the protei ...
BRNO UNIVERSITY OF TECHNOLOGY FACULTY OF
... sequence is determined by the gene coding.2 Proteins vary in molecular mass, ranging approximately from 5000 to more than a million Daltons (Da).1 A scientific discipline dealing with the global analysis of proteins is called proteomics. Proteomics has grown rapidly in a short time and nowadays prov ...
... sequence is determined by the gene coding.2 Proteins vary in molecular mass, ranging approximately from 5000 to more than a million Daltons (Da).1 A scientific discipline dealing with the global analysis of proteins is called proteomics. Proteomics has grown rapidly in a short time and nowadays prov ...
Document
... AA is no longer limiting. If nutrients are not limiting for performance (as indicated in point 2), then it is likely that the growth potential of the animal will be limiting. For an AA supply exactly at the requirement level, the AA and the growth potential will be co-limiting and the requirement ca ...
... AA is no longer limiting. If nutrients are not limiting for performance (as indicated in point 2), then it is likely that the growth potential of the animal will be limiting. For an AA supply exactly at the requirement level, the AA and the growth potential will be co-limiting and the requirement ca ...
Defining the tree of life: Archaea`s place in the evolutionary
... catalytic RNA that self splices. The high degree of similarity in tRNA splicing places Archaea closer to Eucarya. Accordingly DNA replication in Archaea should be similar to that in Eucarya. Eucaryote DNA replication uses family B polymerases as do Archaea. While bacteria contain a very similar fam ...
... catalytic RNA that self splices. The high degree of similarity in tRNA splicing places Archaea closer to Eucarya. Accordingly DNA replication in Archaea should be similar to that in Eucarya. Eucaryote DNA replication uses family B polymerases as do Archaea. While bacteria contain a very similar fam ...
SODIUM-COUPLED TRANSPORTERS FOR KREBS CYCLE
... different proteins are likely to mediate uptake of dicarboxylates in the colon and small intestine (67). Another sodium-dependent transporter for Krebs cycle intermediates has been reported in OK cells (opossum kidney) (30) (Table 1). The OK cell transporter has a relatively low-affinity for citrate ...
... different proteins are likely to mediate uptake of dicarboxylates in the colon and small intestine (67). Another sodium-dependent transporter for Krebs cycle intermediates has been reported in OK cells (opossum kidney) (30) (Table 1). The OK cell transporter has a relatively low-affinity for citrate ...
Identification, Expression and Characterization of Archaeal
... 6.1.3. P. pastoris Culture............................................................................................. 78 6.1.3.1. Preparation of P. pastoris competent cells ................................................. 79 6.1.3.2. Transformation and expression Chop-1SS in P. pastoris (GS115).. ...
... 6.1.3. P. pastoris Culture............................................................................................. 78 6.1.3.1. Preparation of P. pastoris competent cells ................................................. 79 6.1.3.2. Transformation and expression Chop-1SS in P. pastoris (GS115).. ...
3D Structure - Canadian Bioinformatics Workshops
... Arrowsmith CH, Montelione GT, Gerstein M. Nucleic Acids Res 2001 1;29(13):288498 Lecture 3.0 ...
... Arrowsmith CH, Montelione GT, Gerstein M. Nucleic Acids Res 2001 1;29(13):288498 Lecture 3.0 ...
Functional characterization of LePGT1, a membrane
... All these conserved hydrophilic loops face one side of the biological membrane, suggesting that the conserved domains interact to mediate enzyme function, such as substrate recognition. In order to investigate the role of each conserved amino acid, sitedirected mutations were introduced into LePGT1. ...
... All these conserved hydrophilic loops face one side of the biological membrane, suggesting that the conserved domains interact to mediate enzyme function, such as substrate recognition. In order to investigate the role of each conserved amino acid, sitedirected mutations were introduced into LePGT1. ...
Stockholm University
... conformations and what is the corresponding selective pressure? Is there a selective pressure against being too stable or is metastability a neutral emergent property of the evolutionary process? What then are the selective pressures on intrinsically unfolded proteins? Is it possible to derive gener ...
... conformations and what is the corresponding selective pressure? Is there a selective pressure against being too stable or is metastability a neutral emergent property of the evolutionary process? What then are the selective pressures on intrinsically unfolded proteins? Is it possible to derive gener ...
Diverse Effects of Mutations in the Signal Sequence on the Secretion of b-lactamase in Salmonella typhimurium.
... mutants resemble wild-type in that they remain trypsin-inaccessible. One of the latter mutants does produce mature protein, but at a very reduced rate. It thus appears that signalsequence mutations can affect more than one step in the secretion process, and that processing of the signal peptide is n ...
... mutants resemble wild-type in that they remain trypsin-inaccessible. One of the latter mutants does produce mature protein, but at a very reduced rate. It thus appears that signalsequence mutations can affect more than one step in the secretion process, and that processing of the signal peptide is n ...
PDF - The Journal of General Physiology
... Whereas a flux assay using 86Rb+ as a K+ surrogate failed to reveal Letm1dependent 86Rb+ uptake, assays using either 45Ca2+ or a fluorescent Ca2+ indicator indicated that Letm1 mediates Ca2+ transport. Mn2+ inhibited Ca2+ transport, with an apparent Ki of 32 µM; moreover, Letm1 could mediate Ca2+-Mn ...
... Whereas a flux assay using 86Rb+ as a K+ surrogate failed to reveal Letm1dependent 86Rb+ uptake, assays using either 45Ca2+ or a fluorescent Ca2+ indicator indicated that Letm1 mediates Ca2+ transport. Mn2+ inhibited Ca2+ transport, with an apparent Ki of 32 µM; moreover, Letm1 could mediate Ca2+-Mn ...
Protonation and Light Synergistically Convert Plasmalemma Sugar
... assessed after 0.5, 1.5, 3.5, 6, and 9 min. The initial slope was derived from the progress curves and is given in Figure 3. During the 9 min, uptake was observed to be not far from linear. Figure 3, a and b, shows that a curve close to a rectangular hyperbola was obtained only under one set of cond ...
... assessed after 0.5, 1.5, 3.5, 6, and 9 min. The initial slope was derived from the progress curves and is given in Figure 3. During the 9 min, uptake was observed to be not far from linear. Figure 3, a and b, shows that a curve close to a rectangular hyperbola was obtained only under one set of cond ...