PDF - Oxford Academic
... Key words: Auxin, cell cycle, cell synchronization, colchicine, cyclin-dependent kinases, phosphorylation, prophase, retinoblastoma-related protein, roscovitine, immunolocalization. ...
... Key words: Auxin, cell cycle, cell synchronization, colchicine, cyclin-dependent kinases, phosphorylation, prophase, retinoblastoma-related protein, roscovitine, immunolocalization. ...
molecular mechanisms and regulation of k+ transport in higher plants
... Biochemical and reverse genetics analyses directly support this hypothesis in Arabidopsis. The Arabidopsis Shaker family, which comprises nine members, is to date the best-characterized family of plant transport systems. The available information (Table 1) (Figure 2) suggests that these channels are ...
... Biochemical and reverse genetics analyses directly support this hypothesis in Arabidopsis. The Arabidopsis Shaker family, which comprises nine members, is to date the best-characterized family of plant transport systems. The available information (Table 1) (Figure 2) suggests that these channels are ...
Ubiquitin and Plant Viruses, Let`s Play Together!
... has long been limited by the extreme paucity of known targets. It is only recently that a number of viral proteins acting in these processes have been identified and possible mechanisms proposed. It is likely that many more examples remain to be discovered. In some cases, viral proteins appear to usu ...
... has long been limited by the extreme paucity of known targets. It is only recently that a number of viral proteins acting in these processes have been identified and possible mechanisms proposed. It is likely that many more examples remain to be discovered. In some cases, viral proteins appear to usu ...
Identification of the Human Erythrocyte Glucose Transporter (GLUT1
... Follow this and additional works at: http://escholarship.umassmed.edu/gsbs_diss Part of the Life Sciences Commons, and the Medicine and Health Sciences Commons ...
... Follow this and additional works at: http://escholarship.umassmed.edu/gsbs_diss Part of the Life Sciences Commons, and the Medicine and Health Sciences Commons ...
SpyTag and SpyCatcher - Department of Biochemistry
... SpyTag/SpyCatcher has shown a range of intriguing applications, but every nascent technology requires optimisation. In our hands, fusion to SpyTag rarely disturbs function or yield. However, SpyCatcher is a larger fusion, even after we reduced the size from 116 to 84 residues [30]. SpyCatcher is als ...
... SpyTag/SpyCatcher has shown a range of intriguing applications, but every nascent technology requires optimisation. In our hands, fusion to SpyTag rarely disturbs function or yield. However, SpyCatcher is a larger fusion, even after we reduced the size from 116 to 84 residues [30]. SpyCatcher is als ...
Amino Acids, Peptides, and Proteins
... of the amino acids, only the L configuration exists in nature and is used by cells. Some bacteria and fungi synthesize and use D configuration amino acids. The molecules synthesized with these D-form amino acids are often toxic to other living organisms. With these few minor exceptions all of the na ...
... of the amino acids, only the L configuration exists in nature and is used by cells. Some bacteria and fungi synthesize and use D configuration amino acids. The molecules synthesized with these D-form amino acids are often toxic to other living organisms. With these few minor exceptions all of the na ...
An enhanced transient expression system in plants based on
... proteins. In plants, recombinant strains of Agrobacterium tumefaciens can be used for transient expression of genes that have been inserted into the T-DNA region of the bacterial Ti plasmid. A bacterial culture is vacuum-infiltrated into leaves, and upon T-DNA transfer, there is ectopic expression o ...
... proteins. In plants, recombinant strains of Agrobacterium tumefaciens can be used for transient expression of genes that have been inserted into the T-DNA region of the bacterial Ti plasmid. A bacterial culture is vacuum-infiltrated into leaves, and upon T-DNA transfer, there is ectopic expression o ...
Molecular Analysis of the Prostacyclin Receptor’s Interaction with
... lipid modification of CaaX-containing proteins involves the initial isoprenylation of the target Cys through carbon (C)-15 farnesylation or C-20 geranylgeranylation followed by proteolytic cleavage, or –aaXing, of the three terminal residues and subsequent carboxymethylation of the nascent terminal ...
... lipid modification of CaaX-containing proteins involves the initial isoprenylation of the target Cys through carbon (C)-15 farnesylation or C-20 geranylgeranylation followed by proteolytic cleavage, or –aaXing, of the three terminal residues and subsequent carboxymethylation of the nascent terminal ...
Role of cryo-ET in membrane bioenergetics research
... fracture planes that showed both stacked and unstacked thylakoid membranes of chloroplasts revealed a non-random distribution of protein complexes. Proteins on the unstacked membrane surfaces were larger and more dispersed than those on the stacked membranes. Through a combination of immunolabelling ...
... fracture planes that showed both stacked and unstacked thylakoid membranes of chloroplasts revealed a non-random distribution of protein complexes. Proteins on the unstacked membrane surfaces were larger and more dispersed than those on the stacked membranes. Through a combination of immunolabelling ...
Yeast Nutrients Make Fermentations Better
... membranes. 3-5% of the dry cell weight material of yeast is phosphorus, most of which is stored in vacuoles inside the yeast cell. If phosphate is lacking, fermentation troubles can arise due to problems with DNA replication, which results in stuck and incomplete fermentations. Many nutrients sold t ...
... membranes. 3-5% of the dry cell weight material of yeast is phosphorus, most of which is stored in vacuoles inside the yeast cell. If phosphate is lacking, fermentation troubles can arise due to problems with DNA replication, which results in stuck and incomplete fermentations. Many nutrients sold t ...
Complementary Signaling Pathways Regulate the Unfolded Protein
... elegans protein database, and we identified a hypothetical protein encoded by the gene R74.3, which we designated xbp-1. C. elegans XBP-1 has a conserved bZIP domain and shares no amino acid homology with human XBP1 or yeast HAC1 outside of the bZIP region. The xbp-1 gene contains an additional open ...
... elegans protein database, and we identified a hypothetical protein encoded by the gene R74.3, which we designated xbp-1. C. elegans XBP-1 has a conserved bZIP domain and shares no amino acid homology with human XBP1 or yeast HAC1 outside of the bZIP region. The xbp-1 gene contains an additional open ...
Full-Text PDF
... have found that the triolide of R-3HB forms crown ester complexes with alkali metals; Burger and Seebach [39] have shown that oligomers of R-3HB transport alkali and alkaline earth salts across methylene chloride layers in U-tubes; Seebach et al. [40] have demonstrated that PHB of 16 residues or mul ...
... have found that the triolide of R-3HB forms crown ester complexes with alkali metals; Burger and Seebach [39] have shown that oligomers of R-3HB transport alkali and alkaline earth salts across methylene chloride layers in U-tubes; Seebach et al. [40] have demonstrated that PHB of 16 residues or mul ...
Sos recruitment system for the analysis of the
... We found that all of the yeast strains except the negative control grew on SD/gal plates at 37°C but did not grow on glucose plates at 37°C (see Figure 3). These results indicated that all SUMF2 subtypes interacted with both whIL-13 and mhIL-13. However, whether all the SUMF2 subtypes interacted equ ...
... We found that all of the yeast strains except the negative control grew on SD/gal plates at 37°C but did not grow on glucose plates at 37°C (see Figure 3). These results indicated that all SUMF2 subtypes interacted with both whIL-13 and mhIL-13. However, whether all the SUMF2 subtypes interacted equ ...
Isolation of salt sensitive mutants
... Although it is believed that for most of the Earth's history, the salt level of the oceans was much lower than now, all plant species that inhabit the seas, as well as a phylogenetically diverse groups of land plants, are capable of growth and reproduction at salinity levels near or above those foun ...
... Although it is believed that for most of the Earth's history, the salt level of the oceans was much lower than now, all plant species that inhabit the seas, as well as a phylogenetically diverse groups of land plants, are capable of growth and reproduction at salinity levels near or above those foun ...
Synthesis of Substituted Alkanethiols Intended for Protein Immobilization -Chelate Associated Photochemistry (CAP)
... excess of ethylene glycols (known for its protein-repellent properties) to prevent non-specific protein binding, thereby increase the specificity of the sensor surface. With this obtained surface chemistry we hope to contribute to the development of large-scale screening systems and microarrays base ...
... excess of ethylene glycols (known for its protein-repellent properties) to prevent non-specific protein binding, thereby increase the specificity of the sensor surface. With this obtained surface chemistry we hope to contribute to the development of large-scale screening systems and microarrays base ...
CH2O -OCH CH2O- - f.a. #1 f.a.#2 f.a.#3 f.a. = fatty acid.
... by the food industry as emulsifiers for food such as your nice, smooth peanut butter. As the mono- and di-glycerides are completely digestible they are quite harmless (other than being a source of calories). Because of the variable character of the fatty acids the physical state of triacylglycerols ...
... by the food industry as emulsifiers for food such as your nice, smooth peanut butter. As the mono- and di-glycerides are completely digestible they are quite harmless (other than being a source of calories). Because of the variable character of the fatty acids the physical state of triacylglycerols ...
The proton-linked monocarboxylate transporter (MCT) family
... 45 kDa [14]. The unlabelled protein was partially purified following detergent solubilization, and transport activity was measured after reconstitution into proteoliposomes [14,15]. Active fractions were enriched in a 45 kDa protein, and when membrane proteins from DIDS-treated red blood cells were ...
... 45 kDa [14]. The unlabelled protein was partially purified following detergent solubilization, and transport activity was measured after reconstitution into proteoliposomes [14,15]. Active fractions were enriched in a 45 kDa protein, and when membrane proteins from DIDS-treated red blood cells were ...
Vesicle-mediated Protein Transport: Regulatory
... these vps (vacuolar protein sorting defective) mutants missort and secrete vacuolar proteins as their Golgi-modified precursors. Characterization of the products of the VPS genes has provided considerable insight into the molecules and mechanisms involved in the signal-mediated delivery of proteins ...
... these vps (vacuolar protein sorting defective) mutants missort and secrete vacuolar proteins as their Golgi-modified precursors. Characterization of the products of the VPS genes has provided considerable insight into the molecules and mechanisms involved in the signal-mediated delivery of proteins ...
Question paper - Unit F215 - Control, genomes and
... In your answer you should make clear the link between the increased concentration of hydrogen ions and the reduction in the force of contraction of a muscle. ...
... In your answer you should make clear the link between the increased concentration of hydrogen ions and the reduction in the force of contraction of a muscle. ...
Tunicates and not cephalochordates are the closest living relatives
... data. This new phylogenetic scheme prompts a reappraisal of both morphological and palaeontological data and has important implications for the interpretation of developmental and genomic studies in which tunicates and cephalochordates are used as model animals. The introduction of molecular data in ...
... data. This new phylogenetic scheme prompts a reappraisal of both morphological and palaeontological data and has important implications for the interpretation of developmental and genomic studies in which tunicates and cephalochordates are used as model animals. The introduction of molecular data in ...
The Transition from Stiff to Compliant Materials in Squid Beaks
... and dominated by Gly, Ala, His and Asx (where Asx represents Asp and Asn combined) (27, 15, 11, and 7 mole percent, respectively) (Fig. 2, A and B). Only the untanned region differed in composition, with the Asx content being considerably higher and that of the other amino acids being somewhat lower ...
... and dominated by Gly, Ala, His and Asx (where Asx represents Asp and Asn combined) (27, 15, 11, and 7 mole percent, respectively) (Fig. 2, A and B). Only the untanned region differed in composition, with the Asx content being considerably higher and that of the other amino acids being somewhat lower ...
Triphosphatase Related to the Protein Tyrosine Phosphatases
... Transcripts produced by RNA polymerase II are modified at their 59 end by the addition of a 7-methyl guanosine cap. Capping is the earliest modification of mRNA, occurring by the time the transcript is 25–30 nucleotides long (Jove and Manley, 1984; Rasmussen and Lis, 1993). The reaction occurs in th ...
... Transcripts produced by RNA polymerase II are modified at their 59 end by the addition of a 7-methyl guanosine cap. Capping is the earliest modification of mRNA, occurring by the time the transcript is 25–30 nucleotides long (Jove and Manley, 1984; Rasmussen and Lis, 1993). The reaction occurs in th ...
A mitochondrial specific stress response in mammalian cells
... (reviewed by Ma and Hendershot, 2001), having a partly parallel Ire1 pathway (Yoshida et al., 2001; Calfon et al., 2002) and a unique system largely dependent on PERK and ATF6, not found in yeast (Harding et al., 2000; Ye et al., 2000; Yoshida et al., 2000). One of the genes upregulated by UPR is th ...
... (reviewed by Ma and Hendershot, 2001), having a partly parallel Ire1 pathway (Yoshida et al., 2001; Calfon et al., 2002) and a unique system largely dependent on PERK and ATF6, not found in yeast (Harding et al., 2000; Ye et al., 2000; Yoshida et al., 2000). One of the genes upregulated by UPR is th ...