HIS-Select Nickel Affinity Gel (P6611) - Technical - Sigma
... tags will not elute in the pH range of 5.0–6.0. If the tagged recombinant proteins will not elute in this range, try a pH as low as 4.5. V. Cleaning HIS-Select Nickel Affinity Gel for Reuse The affinity gel should be cleaned after every run to ensure that it will function properly on the next use. I ...
... tags will not elute in the pH range of 5.0–6.0. If the tagged recombinant proteins will not elute in this range, try a pH as low as 4.5. V. Cleaning HIS-Select Nickel Affinity Gel for Reuse The affinity gel should be cleaned after every run to ensure that it will function properly on the next use. I ...
Synthetic Physical Interactions Map Kinetochore
... 2008; Rodriguez-Bravo et al. 2014), and roles for SAC proteins in other cellular compartments (Iouk et al. 2002; Wan et al. 2014). Additionally, there is a precedent for checkpoint activation via kinetochore-independent clustering of the Aurora kinase (Campbell and Desai 2013). Therefore, we hypoth ...
... 2008; Rodriguez-Bravo et al. 2014), and roles for SAC proteins in other cellular compartments (Iouk et al. 2002; Wan et al. 2014). Additionally, there is a precedent for checkpoint activation via kinetochore-independent clustering of the Aurora kinase (Campbell and Desai 2013). Therefore, we hypoth ...
Technical White Paper
... diseases and conditions. The SOMAscan assay measures 1129 protein analytes in only 150 µL of serum, plasma or cerebrospinal fluid, or equally small amounts of a variety of other biological matrices. The assay offers exceptional dynamic range, quantifying proteins that span over 8 logs in abundance ( ...
... diseases and conditions. The SOMAscan assay measures 1129 protein analytes in only 150 µL of serum, plasma or cerebrospinal fluid, or equally small amounts of a variety of other biological matrices. The assay offers exceptional dynamic range, quantifying proteins that span over 8 logs in abundance ( ...
Mechanosensitive Channels:
... MscL is dominated by hydrophobic groups, where the majority of the pore hydrophobic or polar, thus very little specificity is conferred when the channel is opened. The small size and observed conductance magnitude and lack of selectivity indicates that MscL should have no major filtering mechanism a ...
... MscL is dominated by hydrophobic groups, where the majority of the pore hydrophobic or polar, thus very little specificity is conferred when the channel is opened. The small size and observed conductance magnitude and lack of selectivity indicates that MscL should have no major filtering mechanism a ...
Starvation Induces Vacuolar Targeting and Degradation of the
... 1997; Didion et al., 1998). Based on their function and regulation, the amino acid permeases are divided into two classes (Sophianopoulou and Diallinas, 1995). Permeases of one class, including the general amino acid permease, GAP1,1 and the proline permease, PUT4, are regulated in response to the a ...
... 1997; Didion et al., 1998). Based on their function and regulation, the amino acid permeases are divided into two classes (Sophianopoulou and Diallinas, 1995). Permeases of one class, including the general amino acid permease, GAP1,1 and the proline permease, PUT4, are regulated in response to the a ...
Rab Proteins and the Organization of Organelle Membrane Domains
... transport protein particle (TRAPP), conserved oligomeric Golgi (COG), dependence on SLY1–20 (Dsl1) and Golgi-associated retrograde protein (GARP) complexes at the Golgi complex, Exocyst at the plasma membrane, and the “class C core vacuole/endosome tethering” (CORVET) and “homotypic fusion and prote ...
... transport protein particle (TRAPP), conserved oligomeric Golgi (COG), dependence on SLY1–20 (Dsl1) and Golgi-associated retrograde protein (GARP) complexes at the Golgi complex, Exocyst at the plasma membrane, and the “class C core vacuole/endosome tethering” (CORVET) and “homotypic fusion and prote ...
Proteomics insights: proteins related to larval attachment and
... that regulate larval attachment and metamorphosis in marine invertebrates have yet to be fully elucidated. Despite the many challenges associated with analysis of the larvae proteome, recent proteomic technologies have been used to address specific questions in larval developmental biology. These an ...
... that regulate larval attachment and metamorphosis in marine invertebrates have yet to be fully elucidated. Despite the many challenges associated with analysis of the larvae proteome, recent proteomic technologies have been used to address specific questions in larval developmental biology. These an ...
Current understanding of fatty acid biosynthesis and the acyl carrier
... machinery that is present in humans evolved from the bacterial systems, and the reactions carried out during the elongation cycle are identical [23]. To this end, FA synthesis is described as it occurs in Escherichia coli, the most studied system to date, and differences to the type I systems are no ...
... machinery that is present in humans evolved from the bacterial systems, and the reactions carried out during the elongation cycle are identical [23]. To this end, FA synthesis is described as it occurs in Escherichia coli, the most studied system to date, and differences to the type I systems are no ...
Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is
... (Fig. 1). In the roots of Fe-deficient plants, the protein spots named C, D, G, V, W, X, and Y were present at higher concentrations than in Fe-sufficient plants (Fig. 1). Protein spots C, D, G, and V had previously been observed to increase during Fe deficiency (Mori et al., 1988; Suzuki et al., 19 ...
... (Fig. 1). In the roots of Fe-deficient plants, the protein spots named C, D, G, V, W, X, and Y were present at higher concentrations than in Fe-sufficient plants (Fig. 1). Protein spots C, D, G, and V had previously been observed to increase during Fe deficiency (Mori et al., 1988; Suzuki et al., 19 ...
Candida antarctica Anders G. Sandström
... out an anaerobic oxidation of carbohydrates to form ethanol. Saccharomyces cerevisiae was used by the Bayer corporation already in the 1930‟s to form a precursor to ephedrine, L-phenylacetylcarbinol via whole-cell biotransformation of benzaldehyde.11 Ever since then, the use of enzymes for biotransf ...
... out an anaerobic oxidation of carbohydrates to form ethanol. Saccharomyces cerevisiae was used by the Bayer corporation already in the 1930‟s to form a precursor to ephedrine, L-phenylacetylcarbinol via whole-cell biotransformation of benzaldehyde.11 Ever since then, the use of enzymes for biotransf ...
The nonenzymatic subunit of pseutarin C, a
... complex” with the nonenzymatic cofactor factor Va (FVa), calcium ions, and phospholipid membranes.1 Phospholipids stimulate prothrombin activation by lowering the Km for prothrombin2-4 and provide a surface on which FXa, FVa, and prothrombin bind in the presence of Ca2⫹, leading to a productive enzy ...
... complex” with the nonenzymatic cofactor factor Va (FVa), calcium ions, and phospholipid membranes.1 Phospholipids stimulate prothrombin activation by lowering the Km for prothrombin2-4 and provide a surface on which FXa, FVa, and prothrombin bind in the presence of Ca2⫹, leading to a productive enzy ...
AN ABSTRACT OF THE THESIS OF Sara R. Fassio for the degree of
... SMF, lack of functional NQR can be expected to reduce SMF-dependent flagella rotation. Since motility is correlated to virulence gene expression (27), we previously proposed that NQR might affect toxT expression via flagella rotation (28). Further characterization of this link, however, revealed tha ...
... SMF, lack of functional NQR can be expected to reduce SMF-dependent flagella rotation. Since motility is correlated to virulence gene expression (27), we previously proposed that NQR might affect toxT expression via flagella rotation (28). Further characterization of this link, however, revealed tha ...
The Pseudomonas syringae HrpJ protein controls the secretion of
... encoded type III-secreted substrates fused to a haemagglutinin (HA) or a FLAG epitope were grown in type IIIinducible medium and separated into cell and supernatant fractions. Interestingly, the two additional putative translocator proteins, the HopAK1 harpin and HrpK1, were not detectable in the su ...
... encoded type III-secreted substrates fused to a haemagglutinin (HA) or a FLAG epitope were grown in type IIIinducible medium and separated into cell and supernatant fractions. Interestingly, the two additional putative translocator proteins, the HopAK1 harpin and HrpK1, were not detectable in the su ...
Protein Structure Prediction Based on Neural Networks
... requires more computing time [3]. In contrast, amino acid sequences may be acquired through very efficient automated, high throughput experimental methods [4]. The amino acid sequences of a vast number of proteins have been identified, while a relatively small number of 3D shapes are known. It follo ...
... requires more computing time [3]. In contrast, amino acid sequences may be acquired through very efficient automated, high throughput experimental methods [4]. The amino acid sequences of a vast number of proteins have been identified, while a relatively small number of 3D shapes are known. It follo ...
Isolation, engineering, and characterization of intracellular
... However, high expression levels of previously reported intrabodies have been required to obtain even limited reductions in htt aggregation. We have used yeast surface display (YSD) of antibodies combined with fluorescence activated cell sorting (FACS) to isolate novel single-chain antibody (scFv) cl ...
... However, high expression levels of previously reported intrabodies have been required to obtain even limited reductions in htt aggregation. We have used yeast surface display (YSD) of antibodies combined with fluorescence activated cell sorting (FACS) to isolate novel single-chain antibody (scFv) cl ...
The Iron-Sulfur Cluster Proteins Isa1 and Isa2 Are Required for the
... a sulfur atom into desthiobiotin, the final step in the biosynthetic pathway, is catalyzed by biotin synthase (Bio2). This mitochondrial protein contains two iron-sulfur (Fe/S) clusters that catalyze the reaction and are thought to act as a sulfur donor. To identify new components of biotin metaboli ...
... a sulfur atom into desthiobiotin, the final step in the biosynthetic pathway, is catalyzed by biotin synthase (Bio2). This mitochondrial protein contains two iron-sulfur (Fe/S) clusters that catalyze the reaction and are thought to act as a sulfur donor. To identify new components of biotin metaboli ...
Preparation of pyruvate for the citric acid cycle Recap 1. We have
... oxidative phosphorylation (leads to more ATP production) 2. Converts to oxaloacetate , which can then shuttle into the synthesize glucose (can also be done from lactate) Anaerobic conditions 3. It is converted to Lactate (animal muscles) 4. It is converted to ethanol (yeast; alcohol fermantation) Py ...
... oxidative phosphorylation (leads to more ATP production) 2. Converts to oxaloacetate , which can then shuttle into the synthesize glucose (can also be done from lactate) Anaerobic conditions 3. It is converted to Lactate (animal muscles) 4. It is converted to ethanol (yeast; alcohol fermantation) Py ...
Sequencing Grade Modified Trypsin, Frozen, Product Information
... Physical Form: Sequencing Grade Modified Trypsin, Frozen, is supplied as a frozen liquid in 50mM acetic acid. Protein Concentration: 0.5 ± 0.05mg/ml (determined by measuring A280). Resuspension Buffer (supplied): Trypsin Resuspension Buffer (V542A) is composed of 50mM acetic acid. This buffer may be ...
... Physical Form: Sequencing Grade Modified Trypsin, Frozen, is supplied as a frozen liquid in 50mM acetic acid. Protein Concentration: 0.5 ± 0.05mg/ml (determined by measuring A280). Resuspension Buffer (supplied): Trypsin Resuspension Buffer (V542A) is composed of 50mM acetic acid. This buffer may be ...
Functions of the cytoplasmic domain of the βPS
... in the wild type. Mitotic clones homozygous for cho are marked by red brick color. (A) An eye containing a cho mysXG43/cho mysXG43 clone. Note that the rhabdomeres are not organized in the regular pattern. (B) An eye containing a cho mysXG43/cho mysXG43; P[mys+]/+ clone. Note the rescue of ommatidia ...
... in the wild type. Mitotic clones homozygous for cho are marked by red brick color. (A) An eye containing a cho mysXG43/cho mysXG43 clone. Note that the rhabdomeres are not organized in the regular pattern. (B) An eye containing a cho mysXG43/cho mysXG43; P[mys+]/+ clone. Note the rescue of ommatidia ...
amino acids - cellbiochem.ca
... L and D: arbitrary. Has nothing to do with the direction of rotation of polarised light by the amino acid.4 CHMI 2227 - E.R. Gauthier, Ph.D. ...
... L and D: arbitrary. Has nothing to do with the direction of rotation of polarised light by the amino acid.4 CHMI 2227 - E.R. Gauthier, Ph.D. ...