Statistical Analysis of Amino Acid Patterns in Transmembrane
... 94 % of the cases, the annotations were marked as potential, possible or probable, indicating that they were identi®ed by hydrophobicity algorithms (Table 1). In order to remove homology, TM sequences with high similarity scores to others were excluded, as described in Methods. The procedure yielded ...
... 94 % of the cases, the annotations were marked as potential, possible or probable, indicating that they were identi®ed by hydrophobicity algorithms (Table 1). In order to remove homology, TM sequences with high similarity scores to others were excluded, as described in Methods. The procedure yielded ...
Merlin and Expanded interact - Development
... 1316 B. M. McCartney and others the actin cytoskeleton through an N-terminal binding region (Xu and Gutmann, 1998). Consistent with this observation, overexpression of Merlin in vertebrate cultured cells causes transient alterations in the F-actin cytoskeleton (Gutmann et al., 1999). Work from seve ...
... 1316 B. M. McCartney and others the actin cytoskeleton through an N-terminal binding region (Xu and Gutmann, 1998). Consistent with this observation, overexpression of Merlin in vertebrate cultured cells causes transient alterations in the F-actin cytoskeleton (Gutmann et al., 1999). Work from seve ...
THE QUEST FOR A MESSAGE: BUDDING YEAST, A MODEL
... global effect on splicing of a large set of splicing factors (see [52] for a review). In yeast, a certain number of them can be easily deleted from the genome, with only minor effects on cell viability in standard laboratory conditions [53]. Other splicing factors however are essential, and cannot b ...
... global effect on splicing of a large set of splicing factors (see [52] for a review). In yeast, a certain number of them can be easily deleted from the genome, with only minor effects on cell viability in standard laboratory conditions [53]. Other splicing factors however are essential, and cannot b ...
Sequence and evolutionary analysis of the human trypsin subfamily
... Although only seven residues showed 100% conservation, an additional 15 showed almost complete conservation (95%+). Eight of these were within close proximity to one of the three catalytic residues. Indeed, all six residues of the GDSGGP motif around Ser195 showed at least 95% conservation. Of the r ...
... Although only seven residues showed 100% conservation, an additional 15 showed almost complete conservation (95%+). Eight of these were within close proximity to one of the three catalytic residues. Indeed, all six residues of the GDSGGP motif around Ser195 showed at least 95% conservation. Of the r ...
Antimicrobial Agents Produced by Yeasts
... killer cells have developed to circumvent the lethal effects of the toxin they produce [16]. Killer yeasts isolated from flowers of Indian medicinal plants and their killer toxin was determined on sensitive yeast cells as well as fungal pathogens. The toxin of Saccharomyces cerevisiae and Pichia klu ...
... killer cells have developed to circumvent the lethal effects of the toxin they produce [16]. Killer yeasts isolated from flowers of Indian medicinal plants and their killer toxin was determined on sensitive yeast cells as well as fungal pathogens. The toxin of Saccharomyces cerevisiae and Pichia klu ...
Co-Expression of Arabidopsis thaliana Phytochelatin Synthase and Treponema denticola
... 2005; Stauder et al., 2005). Although little is known about the bioavailability of thioarsenics, they are reported to be less toxic than arsenite (Rader et al., 2004). In some eukaryotes, incorporation of sulfide to form a more stable high molecular weight PC–metal–sulfide complex in the vacuole has a ...
... 2005; Stauder et al., 2005). Although little is known about the bioavailability of thioarsenics, they are reported to be less toxic than arsenite (Rader et al., 2004). In some eukaryotes, incorporation of sulfide to form a more stable high molecular weight PC–metal–sulfide complex in the vacuole has a ...
Lactococcus lactis as expression host for the biosynthetic
... quantum yield (Q) are highly sensitive parameters for monitoring changes in the structure and dynamics of a protein. In numerous studies, the intrinsic protein fluorescence was used to monitor processes like enzyme kinetics, protein–ligand interactions or protein (un)folding. Modern molecular biolog ...
... quantum yield (Q) are highly sensitive parameters for monitoring changes in the structure and dynamics of a protein. In numerous studies, the intrinsic protein fluorescence was used to monitor processes like enzyme kinetics, protein–ligand interactions or protein (un)folding. Modern molecular biolog ...
uncorrected proof
... closed conformation in the crystal form L with one molecule per asymmetric unit as well as in the crystal form H, which hosts two molecules per asymmetric unit. Structural comparison of the a-actinin ABD with plectin (Figure 2(a); Table 2) and fimbrin (Table 2) gives an r.m.s. deviation of 1.46 Å ( ...
... closed conformation in the crystal form L with one molecule per asymmetric unit as well as in the crystal form H, which hosts two molecules per asymmetric unit. Structural comparison of the a-actinin ABD with plectin (Figure 2(a); Table 2) and fimbrin (Table 2) gives an r.m.s. deviation of 1.46 Å ( ...
Identification of genes expressed in cultures of E. coli lysogens
... Additional file 2: Table S2). Four genes identified by 2D-PAGE, P1, P4, P5 and P6, visibly follow the same expression pattern as the genes expressed during the lytic cycle and accordingly the increase in gene copy number is significant (p-value < 0.05) after 50 or 60 min of recovery from exposure to ...
... Additional file 2: Table S2). Four genes identified by 2D-PAGE, P1, P4, P5 and P6, visibly follow the same expression pattern as the genes expressed during the lytic cycle and accordingly the increase in gene copy number is significant (p-value < 0.05) after 50 or 60 min of recovery from exposure to ...
Lecture 3 Isoelectric Focusing
... It will become -ve (pH>pI and migrate to the anode (+electrode attracts -ion)) As it passes through the gel, eg when it reaches pH 9 region, it becomes less -vely charged (pH not so much >pI) (+electrode does not attract protein so much – protein slows down) When reaches the pH 8 region, protein cha ...
... It will become -ve (pH>pI and migrate to the anode (+electrode attracts -ion)) As it passes through the gel, eg when it reaches pH 9 region, it becomes less -vely charged (pH not so much >pI) (+electrode does not attract protein so much – protein slows down) When reaches the pH 8 region, protein cha ...
Growing Membranes, Sustaining Cells
... and connected by transporters to other pathways. Instead, some pathways operate across two or more compartments. In other words, some pathways are segmented into pieces operating in different compartments in eukaryotic cells, where membrane transporters become part of the pathway itself, as is well ...
... and connected by transporters to other pathways. Instead, some pathways operate across two or more compartments. In other words, some pathways are segmented into pieces operating in different compartments in eukaryotic cells, where membrane transporters become part of the pathway itself, as is well ...
Uptake and assimilation of nitrogen from solutions containing
... the 24 h 15N labelling period were calculated by assuming that plants in the various N source treatments were identical. This assumption is reasonable since all plants were raised on the same N source, NH4NO3, before the labelling period. Data from the three single source treatments were combined. F ...
... the 24 h 15N labelling period were calculated by assuming that plants in the various N source treatments were identical. This assumption is reasonable since all plants were raised on the same N source, NH4NO3, before the labelling period. Data from the three single source treatments were combined. F ...
The f ructokinase f rom Rhizobium leguminosarum
... The Rhizobium leguminosarum bv. trifolii BAL fructokinase (frk) gene was isolated on a 2 4 kb BamHl fragment from the cosmid pLA72 by complementation analysis of the Tn5-induced frk mutant BAL79, and confirmed by hybridization analysis. The nucleotide sequence of the frk gene was found to contain an ...
... The Rhizobium leguminosarum bv. trifolii BAL fructokinase (frk) gene was isolated on a 2 4 kb BamHl fragment from the cosmid pLA72 by complementation analysis of the Tn5-induced frk mutant BAL79, and confirmed by hybridization analysis. The nucleotide sequence of the frk gene was found to contain an ...
Molecular events associated with acquisition of heat
... Abstract: The heat shock response is an inducible protective system of all living cells. It simultaneously induces both heat shock proteins and an increased capacity for the cell to withstand potentially lethal temperatures (an increased thermotolerance). This has lead to the suspicion that these tw ...
... Abstract: The heat shock response is an inducible protective system of all living cells. It simultaneously induces both heat shock proteins and an increased capacity for the cell to withstand potentially lethal temperatures (an increased thermotolerance). This has lead to the suspicion that these tw ...
Development of Surface Modified Magnetic PLGA Nanoparticles
... sample forms observable coagulates or flocculates after a few hours of preparation which increases with time. This is also supported by our ZP values shown in Table 2. In all cases, visual observation strongly supported the ZP values obtained in this study. The addition of poloxamer leads to an incr ...
... sample forms observable coagulates or flocculates after a few hours of preparation which increases with time. This is also supported by our ZP values shown in Table 2. In all cases, visual observation strongly supported the ZP values obtained in this study. The addition of poloxamer leads to an incr ...
dbPSP: a curated database for protein phosphorylation sites in
... its critical roles in various cellular processes such as signal transduction. Thus, an integrative data resource of the prokaryotic phosphorylation will be useful for further analysis. In this study, we presented a curated database of phosphorylation sites in prokaryotes (dbPSP, Database URL: http:/ ...
... its critical roles in various cellular processes such as signal transduction. Thus, an integrative data resource of the prokaryotic phosphorylation will be useful for further analysis. In this study, we presented a curated database of phosphorylation sites in prokaryotes (dbPSP, Database URL: http:/ ...
View - BioOne
... Nitrogen-fixing cyanobacteria are vital photosynthetic microorganisms that contribute to soil fertility by fixing atmospheric nitrogen and are also important for maintaining ecosystem stability. These microorganisms can be very sensitive to herbicides because they possess many characteristics of hig ...
... Nitrogen-fixing cyanobacteria are vital photosynthetic microorganisms that contribute to soil fertility by fixing atmospheric nitrogen and are also important for maintaining ecosystem stability. These microorganisms can be very sensitive to herbicides because they possess many characteristics of hig ...
Inner Blood-Retinal Barrier Transporters: Relevance to
... Tachikawa, 2009). In general, the membrane transporter is the 12 membrane-spanning membrane protein widely found in a variety of species ranging from bacteria to humans (Kubo et al., 2000; Kubo et al., 2005), and it is protein responsible for carrier-mediated transport. ATP-binding cassette (ABC) tr ...
... Tachikawa, 2009). In general, the membrane transporter is the 12 membrane-spanning membrane protein widely found in a variety of species ranging from bacteria to humans (Kubo et al., 2000; Kubo et al., 2005), and it is protein responsible for carrier-mediated transport. ATP-binding cassette (ABC) tr ...
PDF - University of California, San Francisco
... Reductase-Hydrophobicity plots of the amino acid sequence were FIG. 1. Amino acid sequence and domain map of hamster generated by the method of Kyte and Doolittle (10). The hydrophoHMG-CoA reductase. Panel A , amino acid sequence. Amino acid bicity values used for each amino acid were those of Eisen ...
... Reductase-Hydrophobicity plots of the amino acid sequence were FIG. 1. Amino acid sequence and domain map of hamster generated by the method of Kyte and Doolittle (10). The hydrophoHMG-CoA reductase. Panel A , amino acid sequence. Amino acid bicity values used for each amino acid were those of Eisen ...
a-Aminoadipate aminotransferase from an extremely
... To characterize the lysN gene product, we expressed the gene in E. coli and obtained a purified preparation of LysN by using a T7 promoter expression system. LysN was accumulated as insoluble inclusion bodies when the E. coli cells carrying pETLysN7 were cultured in the presence of 1 mM IPTG at 37 u ...
... To characterize the lysN gene product, we expressed the gene in E. coli and obtained a purified preparation of LysN by using a T7 promoter expression system. LysN was accumulated as insoluble inclusion bodies when the E. coli cells carrying pETLysN7 were cultured in the presence of 1 mM IPTG at 37 u ...
Identification of Genes Involved in the Assembly and Biosynthesis of... N
... Macario, 2008). Recently, it has been proposed that gut methanogens play a role in obesity (Zhang et al., 2009) while some patients with diseases such as diverticulitis and colon cancer have elevated numbers of archaea within their digestive tract (Eckburg et al., 2003). The most well understood dis ...
... Macario, 2008). Recently, it has been proposed that gut methanogens play a role in obesity (Zhang et al., 2009) while some patients with diseases such as diverticulitis and colon cancer have elevated numbers of archaea within their digestive tract (Eckburg et al., 2003). The most well understood dis ...
Identification of Saccharomyces cerevisiae proteins that bind to
... solenoid model in that alternate nucleosomes interact with each other and sequential nucleosomes (nucleosomes connected by linker DNA) are positioned opposite each other. This way the linker DNA can remain more rigid and a secondary structure resembling a “two start” helix is formed (Dorigo et al. 2 ...
... solenoid model in that alternate nucleosomes interact with each other and sequential nucleosomes (nucleosomes connected by linker DNA) are positioned opposite each other. This way the linker DNA can remain more rigid and a secondary structure resembling a “two start” helix is formed (Dorigo et al. 2 ...
full text
... sustained muscle contraction. Another interesting point to be discussed is the higher Ki value reported for calcium uptake compared to that obtained for Ca-ATPase activity. It should be noted that CaATPase activity was measured in the presence of a calcium ionophore while calcium uptake was measured ...
... sustained muscle contraction. Another interesting point to be discussed is the higher Ki value reported for calcium uptake compared to that obtained for Ca-ATPase activity. It should be noted that CaATPase activity was measured in the presence of a calcium ionophore while calcium uptake was measured ...