Amino Acids and Proteins - KSU Faculty Member websites
... D. Amino acids with basic side chains The side chains of the basic amino acids accept protons . At physiologic pH the side chains of lysine and arginine are fully ionized and positively charged. In contrast, histidine is weakly basic, and the free amino acid is largely uncharged at physiologic pH. H ...
... D. Amino acids with basic side chains The side chains of the basic amino acids accept protons . At physiologic pH the side chains of lysine and arginine are fully ionized and positively charged. In contrast, histidine is weakly basic, and the free amino acid is largely uncharged at physiologic pH. H ...
Cell-to-cell communication and virulence in Vibrio anguillarum
... settings due to the frequency of outbreaks, geographic distribution, and numbers of fish species affected [70]. Over 50 fish species in at least 17 countries have been reported with disease caused by V. anguillarum [71]. Vibrio anguillarum is found as normal and commensal flora on many fish species ...
... settings due to the frequency of outbreaks, geographic distribution, and numbers of fish species affected [70]. Over 50 fish species in at least 17 countries have been reported with disease caused by V. anguillarum [71]. Vibrio anguillarum is found as normal and commensal flora on many fish species ...
Expanding the Genetic Code
... of amino acids in the code,[1–3] it is clear that proteins require additional chemical groups to carry out their natural functions. These groups are provided through posttranslational modifications including phosphorylation, methylation, acetylation, and hydroxylation; cofactors; and in rare cases, ...
... of amino acids in the code,[1–3] it is clear that proteins require additional chemical groups to carry out their natural functions. These groups are provided through posttranslational modifications including phosphorylation, methylation, acetylation, and hydroxylation; cofactors; and in rare cases, ...
Nucleotide Sequence of fruA, the Gene Specifying Enzyme IIfru of
... a 2.5 kb PvuII restriction fragment spanningfruA+,cloned on a plasmid, was determined. This fragment contained three open reading frames (ORFs) but only one complete ORF, 1689 base pairs long, which was preceded by a well-defined Shine-Dalgarno sequence and ended with a rho-independent transcription ...
... a 2.5 kb PvuII restriction fragment spanningfruA+,cloned on a plasmid, was determined. This fragment contained three open reading frames (ORFs) but only one complete ORF, 1689 base pairs long, which was preceded by a well-defined Shine-Dalgarno sequence and ended with a rho-independent transcription ...
Dissociation of a ll0-kD Peripheral Membrane Protein from the Golgi
... of membrane-bounded compartmentalization. One potential mechanism by which BFA could act is through the disruption of underlying structural elements of the Golgi apparatus, To explore this possibility further we examined the effect of BFA on several cytoplasmically oriented proteins associated with ...
... of membrane-bounded compartmentalization. One potential mechanism by which BFA could act is through the disruption of underlying structural elements of the Golgi apparatus, To explore this possibility further we examined the effect of BFA on several cytoplasmically oriented proteins associated with ...
Modeling leaf venation patterns Anne-Gaelle Rolland
... Models of venation formation in response to auxin Two biologically plausible hypotheses have been proposed to explain how veins are formed. The first one, named the canalization hypothesis (Sachs, 1981), was based on a series of careful experiments (Sachs, 1981). It predicts that as auxin flows thro ...
... Models of venation formation in response to auxin Two biologically plausible hypotheses have been proposed to explain how veins are formed. The first one, named the canalization hypothesis (Sachs, 1981), was based on a series of careful experiments (Sachs, 1981). It predicts that as auxin flows thro ...
Developmentally regulated, alternative splicing of the Rpn10 gene
... amino acid residues, respectively. Thus, each Rpn10 form has a unique structural feature that distinguishes one from another. To understand how the multiple Rpn10 cDNAs are produced, we isolated genomic clones for Rpn10 from a mouse l phage library. Only one Rpn10 gene, designated Psmd4, was identi® ...
... amino acid residues, respectively. Thus, each Rpn10 form has a unique structural feature that distinguishes one from another. To understand how the multiple Rpn10 cDNAs are produced, we isolated genomic clones for Rpn10 from a mouse l phage library. Only one Rpn10 gene, designated Psmd4, was identi® ...
Raw Glycerol as Substrate for the Production of Yeast Biomass
... C. lipolytica NRRL Y-1095 showed the highest values (1.9 0.1 g/L and 1.8 0.1 g/L, respectively) for analytical grade glycerol, differing statistically from the other yeast strains. In the medium containing glycerol derived from biodiesel production, there was no statistically significant differe ...
... C. lipolytica NRRL Y-1095 showed the highest values (1.9 0.1 g/L and 1.8 0.1 g/L, respectively) for analytical grade glycerol, differing statistically from the other yeast strains. In the medium containing glycerol derived from biodiesel production, there was no statistically significant differe ...
Molecular Characterisation of the 76 kDa Iron
... potential presence of the 76 kDa polypeptide, or a homologue of it, in chloroplasts, various protein fractions of spinach leaf chloroplasts were resolved by SDS-PAGE, blotted, and probed with a polyclonal antiserum against the 78 kDa subunit of mitochondrial complex I from N. crassa (Friedrich et al ...
... potential presence of the 76 kDa polypeptide, or a homologue of it, in chloroplasts, various protein fractions of spinach leaf chloroplasts were resolved by SDS-PAGE, blotted, and probed with a polyclonal antiserum against the 78 kDa subunit of mitochondrial complex I from N. crassa (Friedrich et al ...
EP 1790660 B1
... [0020] The method will typically involve the steps of: obtaining nucleic acid encoding a protein of the invention; manipulating said nucleic acid to remove at least one domain from within the protein. The resulting nucleic acid may be inserted into an expression vector, or may already be part of an ...
... [0020] The method will typically involve the steps of: obtaining nucleic acid encoding a protein of the invention; manipulating said nucleic acid to remove at least one domain from within the protein. The resulting nucleic acid may be inserted into an expression vector, or may already be part of an ...
Contributions of direct incorporation from diet and microbial amino
... is expected, as the use of premanufactured carbon skeletons reduces the costs of synthesis (Horton et al. 2002). An implication of these observations is that contrary to the predictions of mixing models, the carbon isotope (d13C) value of animal protein in tissues should resemble not that of bulk di ...
... is expected, as the use of premanufactured carbon skeletons reduces the costs of synthesis (Horton et al. 2002). An implication of these observations is that contrary to the predictions of mixing models, the carbon isotope (d13C) value of animal protein in tissues should resemble not that of bulk di ...
Sodium-Coupled Neurotransmitter Transporters Baruch I. Kanner* and Elia Zomot
... of the membrane electric field (Figure 2B). In some transporters, the internal gate can briefly open even in the absence of substrate. This results in steady-state leak currents, carried by sodium or other ions (see for example refs 32 and 33). Sometimes the ions leaking through the transporter are ...
... of the membrane electric field (Figure 2B). In some transporters, the internal gate can briefly open even in the absence of substrate. This results in steady-state leak currents, carried by sodium or other ions (see for example refs 32 and 33). Sometimes the ions leaking through the transporter are ...
Electron microscopy in structural studies of Photosystem II
... 1. Projection contours of the particles associated with EFs, PFs and ESs planes obtained by analysis of EM images of 2-D particle arrays from freeze-fracture and freeze-etching experiments: (a) 2-D array of EFs particles from spinach (Simpson 1978). (b) 2-D array of EFs particles from Arabidopsis (T ...
... 1. Projection contours of the particles associated with EFs, PFs and ESs planes obtained by analysis of EM images of 2-D particle arrays from freeze-fracture and freeze-etching experiments: (a) 2-D array of EFs particles from spinach (Simpson 1978). (b) 2-D array of EFs particles from Arabidopsis (T ...
Vesicular transport of newly synthesized opsin from the Golgi
... Prior autoradiographic and radiobiochemical studies showed that most of the radiolabeled protein migrated from the Golgi past the ellipsoid—a mitochondria rich domain between the nucleus and the ROS—to arrive eventually in newly formed basal.ROS disks.17"19 Photoreceptor inner and outer segments are ...
... Prior autoradiographic and radiobiochemical studies showed that most of the radiolabeled protein migrated from the Golgi past the ellipsoid—a mitochondria rich domain between the nucleus and the ROS—to arrive eventually in newly formed basal.ROS disks.17"19 Photoreceptor inner and outer segments are ...
Potential mechanisms involved in the absorptive transport of
... (Bridges et al., 2004; Bridges and Zalups, 2004; Cannon et al., 2001). Since both Cd and Hg are group IIB metals and both have a high binding affinity for sulfhydryl groups, it is possible that lowmolecular-weight thiol S-conjugates of Cd2+ utilize one or more of the same transporters involved in the ...
... (Bridges et al., 2004; Bridges and Zalups, 2004; Cannon et al., 2001). Since both Cd and Hg are group IIB metals and both have a high binding affinity for sulfhydryl groups, it is possible that lowmolecular-weight thiol S-conjugates of Cd2+ utilize one or more of the same transporters involved in the ...
Proteomics - Technologies, Markets and Companies Brochure
... Matrix-Assisted Laser Desorption Mass Spectrometry (MALDI-MS) has become a widely used method for determination of biomolecules including peptides, proteins. Functional proteomics technologies include yeast two-hybrid system for studying protein- protein interactions. Establishing a proteomics platf ...
... Matrix-Assisted Laser Desorption Mass Spectrometry (MALDI-MS) has become a widely used method for determination of biomolecules including peptides, proteins. Functional proteomics technologies include yeast two-hybrid system for studying protein- protein interactions. Establishing a proteomics platf ...
INHIBITION OF OAT3-MEDIATED RENAL UPTAKE AS A
... responsible for the hepatic uptake of fexofenadine (Drug Metab Dispos 33:1477–1481, 2005). The Ki values were determined to be 1.30 and 130 M for hOAT3 and hOATP1B3, respectively, with Hill coefficients of 0.76 and 0.64, respectively. The Ki value of probenecid for hOAT3, but not for hOATP1B3, was ...
... responsible for the hepatic uptake of fexofenadine (Drug Metab Dispos 33:1477–1481, 2005). The Ki values were determined to be 1.30 and 130 M for hOAT3 and hOATP1B3, respectively, with Hill coefficients of 0.76 and 0.64, respectively. The Ki value of probenecid for hOAT3, but not for hOATP1B3, was ...
Plant blue-light receptors - Molecular, Cell, and Developmental
... Since the discovery of plant cryptochromes, this type of photolyase-like pigment has also been found in animals. For example, human and mouse each have two cryptochromes (hCRY1 and hCRY2, and mCry1 and mCry2)47,48, and Drosophila has one cryptochrome (dCRY)49. Animal cryptochromes are also flavoprot ...
... Since the discovery of plant cryptochromes, this type of photolyase-like pigment has also been found in animals. For example, human and mouse each have two cryptochromes (hCRY1 and hCRY2, and mCry1 and mCry2)47,48, and Drosophila has one cryptochrome (dCRY)49. Animal cryptochromes are also flavoprot ...
FEBS Letters
... led to the identification of a 26.5 kbp locus on which a dxr homologous gene and two adjacent open reading frames organized in one operon were localized by DNA sequencing. The functionality of the gene was demonstrated expressing the gene in Escherichia coli and using the purified gene product in a ...
... led to the identification of a 26.5 kbp locus on which a dxr homologous gene and two adjacent open reading frames organized in one operon were localized by DNA sequencing. The functionality of the gene was demonstrated expressing the gene in Escherichia coli and using the purified gene product in a ...
Studies on legume receptors for Nod and Myc symbiotic signals
... large number of plant viruses and other pathogens such as bacteria, oomycetes and fungi and thus is widely used in plant virology and for host–pathogen interaction studies. For the reason that its large leaves can be easily transformed with high efficiency with Agrobacterium tumefaciens, N. benthami ...
... large number of plant viruses and other pathogens such as bacteria, oomycetes and fungi and thus is widely used in plant virology and for host–pathogen interaction studies. For the reason that its large leaves can be easily transformed with high efficiency with Agrobacterium tumefaciens, N. benthami ...
Microscopy Microanalysis
... Different mechanisms, which could induce precipitation in natural habitats by bacteria, have been proposed and active and passive roles of bacteria in these processes have been suggested ~Erlich, 1996; Morita, 1980; Novitsky, 1981!. However, in many cases their precise role in carbonate formation is ...
... Different mechanisms, which could induce precipitation in natural habitats by bacteria, have been proposed and active and passive roles of bacteria in these processes have been suggested ~Erlich, 1996; Morita, 1980; Novitsky, 1981!. However, in many cases their precise role in carbonate formation is ...
A nucleus-encoded chloroplast protein regulated by iron availability
... previously observed for other C. reinhardtii proteins such as TAB1 (Rahire et al., 2012) or RAA1 (Merendino et al., 2006). This could be due to the presence of long hydrophobic stretches and the large size of these proteins. It is noteworthy that different levels of accumulation of TAA1 were observe ...
... previously observed for other C. reinhardtii proteins such as TAB1 (Rahire et al., 2012) or RAA1 (Merendino et al., 2006). This could be due to the presence of long hydrophobic stretches and the large size of these proteins. It is noteworthy that different levels of accumulation of TAA1 were observe ...