amino acids - cellbiochem.ca
... L and D: arbitrary. Has nothing to do with the direction of rotation of polarised light by the amino acid.4 CHMI 2227 - E.R. Gauthier, Ph.D. ...
... L and D: arbitrary. Has nothing to do with the direction of rotation of polarised light by the amino acid.4 CHMI 2227 - E.R. Gauthier, Ph.D. ...
Acquisition, Conservation, and Loss of Dual
... distinguish between organelle-specific signals. In addition to dual targeting to mitochondria and plastids, dual targeting of proteins to mitochondria and peroxisomes has also been reported (Carrie et al., 2008, 2009b). The mechanism of targeting differs in that proteins dual targeted to mitochondria ...
... distinguish between organelle-specific signals. In addition to dual targeting to mitochondria and plastids, dual targeting of proteins to mitochondria and peroxisomes has also been reported (Carrie et al., 2008, 2009b). The mechanism of targeting differs in that proteins dual targeted to mitochondria ...
Alpha and beta subunits of pyruvate dehydrogenase E1
... everted in the presence of a potential host, it can adhere to or penetrate the membrane of the host cell, which in turn allows the infectious sporoplasm to pass though the tube and into the host [2]. Microsporidia are one of the more highly adapted groups of eukaryotes known: practically every major ...
... everted in the presence of a potential host, it can adhere to or penetrate the membrane of the host cell, which in turn allows the infectious sporoplasm to pass though the tube and into the host [2]. Microsporidia are one of the more highly adapted groups of eukaryotes known: practically every major ...
Introduction to Protein Structure
... the tertiary structure of a protein resides in the chemistry of its amino acid sequence. […] It was demonstrated that, after cleavage of disulfide bonds and disruption of tertiary structure, many proteins could spontaneously refold to their native forms. This work resulted in general acceptance of t ...
... the tertiary structure of a protein resides in the chemistry of its amino acid sequence. […] It was demonstrated that, after cleavage of disulfide bonds and disruption of tertiary structure, many proteins could spontaneously refold to their native forms. This work resulted in general acceptance of t ...
mRNA surveillance: the perfect persist
... characterized, although apparent homologs of SMG-5 exist (Clissold and Ponting, 2000). Upf proteins: factors involved in NMD Upf1p is the best-studied factor in NMD. It is a cytoplasmic protein that has a cysteine-histidine-rich region at its NThree interacting trans-acting factors, Upf1p, Upf2p, an ...
... characterized, although apparent homologs of SMG-5 exist (Clissold and Ponting, 2000). Upf proteins: factors involved in NMD Upf1p is the best-studied factor in NMD. It is a cytoplasmic protein that has a cysteine-histidine-rich region at its NThree interacting trans-acting factors, Upf1p, Upf2p, an ...
biochemical model for enhanced biological phosphorus removal
... Three major mechanisms are used by most bacteria to translocate protons and maintain a pmf (see Fig. 4). The first one is of major importance and makes use of the cytoplasmic membrane-bound electron transport chain to expel H + from the cell when carbon substrates and an electron acceptor, mainly ox ...
... Three major mechanisms are used by most bacteria to translocate protons and maintain a pmf (see Fig. 4). The first one is of major importance and makes use of the cytoplasmic membrane-bound electron transport chain to expel H + from the cell when carbon substrates and an electron acceptor, mainly ox ...
western blotting - New England Biolabs GmbH
... minutes. CST has also tested the Trans-blot® Turbo™ transfer system from Bio-Rad. This system works well for the transfer of both low and high molecular weight proteins and requires less buffer than wet transfer, however in some instances it can yield higher background staining. Shown here (A) is a ...
... minutes. CST has also tested the Trans-blot® Turbo™ transfer system from Bio-Rad. This system works well for the transfer of both low and high molecular weight proteins and requires less buffer than wet transfer, however in some instances it can yield higher background staining. Shown here (A) is a ...
ref. #27 of the TIBS article
... machinery (Kutay et al., 1995), and it can be glycosylated on its lumenal, C-terminal tail even when the single transmembrane domain is replaced by poly-Leu stretches (Whitley et al., 1996a). We thus determined MGDC vaules for four different constructs derived from human synaptobrevin 2 with poly-Le ...
... machinery (Kutay et al., 1995), and it can be glycosylated on its lumenal, C-terminal tail even when the single transmembrane domain is replaced by poly-Leu stretches (Whitley et al., 1996a). We thus determined MGDC vaules for four different constructs derived from human synaptobrevin 2 with poly-Le ...
Simultaneous Alignment and Folding of Protein Sequences
... structure. Moreover, for proteins, there is no clear chemical basis for compensatory mutations [11], the energy models that define β-strand pairings are more complex, and the larger residue alphabet vastly increases the complexity of the problem. This class of problems is also different than any tha ...
... structure. Moreover, for proteins, there is no clear chemical basis for compensatory mutations [11], the energy models that define β-strand pairings are more complex, and the larger residue alphabet vastly increases the complexity of the problem. This class of problems is also different than any tha ...
Copyright Information of the Article Published Online
... entered eukaryotic cells and became organelles. However, this theory has not always been accepted by the scientific community; it was first proposed for plastids at the beginning of the 20th century, but was only accepted for mitochondria for a few years before being rejected by cell biologists. It ...
... entered eukaryotic cells and became organelles. However, this theory has not always been accepted by the scientific community; it was first proposed for plastids at the beginning of the 20th century, but was only accepted for mitochondria for a few years before being rejected by cell biologists. It ...
PDF of original
... in the presence of limiting concentrations of oligonucleotides (0.50 or 0.12 m/~moles of oligonucleotides containing U or A, respectively) compared to either UpUpU, for C14Phe-sRNA; or ApApA, for C14-Lys-sRNA (each assumed to be 100%). Data ave from Rottman and Nirenberg (1966) except results with o ...
... in the presence of limiting concentrations of oligonucleotides (0.50 or 0.12 m/~moles of oligonucleotides containing U or A, respectively) compared to either UpUpU, for C14Phe-sRNA; or ApApA, for C14-Lys-sRNA (each assumed to be 100%). Data ave from Rottman and Nirenberg (1966) except results with o ...
Processing of a Wheat Light-Harvesting Chlorophyll a/b Protein
... were incubated with pea chloroplasts, which were then either treated or not treated with thermolysin before lysis. Tl~ermolysin has been shown to preferentially digest outer membrane proteins (10, 20) that have not entered the organelle. The membrane and soluble fractions of the chloroplast were sep ...
... were incubated with pea chloroplasts, which were then either treated or not treated with thermolysin before lysis. Tl~ermolysin has been shown to preferentially digest outer membrane proteins (10, 20) that have not entered the organelle. The membrane and soluble fractions of the chloroplast were sep ...
HIC-5 Is a Novel Repressor of Lymphoid Enhancer Factor/T
... Activation of Wnt/-catenin target genes is regulated by a heterodimer of -catenin and the high mobility group box transcription factors of the lymphoid enhancer factor (LEF)/T-cell factor (TCF) family. In vertebrates, four LEF/TCF family members have been identified. They all contain a conserved  ...
... Activation of Wnt/-catenin target genes is regulated by a heterodimer of -catenin and the high mobility group box transcription factors of the lymphoid enhancer factor (LEF)/T-cell factor (TCF) family. In vertebrates, four LEF/TCF family members have been identified. They all contain a conserved  ...
The biosynthesis of peptidoglycan lipid
... a-helices (Fig. 4). A largely hydrophobic 30 Å depth cleft was observed at the protein surface whose entrance carries several positively charged residues as well as a ‘structural P loop’ motif that is characteristic of phosphate recognition enzymes. A flexible domain located in the vicinity of the ...
... a-helices (Fig. 4). A largely hydrophobic 30 Å depth cleft was observed at the protein surface whose entrance carries several positively charged residues as well as a ‘structural P loop’ motif that is characteristic of phosphate recognition enzymes. A flexible domain located in the vicinity of the ...
Diacylglycerol kinases - University of Toronto Mississauga
... All DGKs have at least two cysteine-rich regions homologous to the C1A and C1B motifs of PKCs [26]. In theory, these domains may bind DAG, perhaps localizing DGKs to where DAG accumulates. However, no DGK C1 domain has so far been conclusively shown to bind DAG. In fact, structural predictions sugge ...
... All DGKs have at least two cysteine-rich regions homologous to the C1A and C1B motifs of PKCs [26]. In theory, these domains may bind DAG, perhaps localizing DGKs to where DAG accumulates. However, no DGK C1 domain has so far been conclusively shown to bind DAG. In fact, structural predictions sugge ...
–potassium intersection: membranes, The nitrogen metabolism, and mechanism Review
... 200–500 μM. At higher K+ and NH4+ concentrations (5 and 10 mM, respectively), however, it was clearly demonstrated in a study on barley roots that steady-state NH4+ supply can indeed inhibit LATS-range K+ transport (Coskun et al. 2013a). In this study, removal of NH4+ during measurement of 42K+ upta ...
... 200–500 μM. At higher K+ and NH4+ concentrations (5 and 10 mM, respectively), however, it was clearly demonstrated in a study on barley roots that steady-state NH4+ supply can indeed inhibit LATS-range K+ transport (Coskun et al. 2013a). In this study, removal of NH4+ during measurement of 42K+ upta ...
The AMP-activated protein kinase pathway – new
... of a catalytic α subunit and regulatory β and γ subunits (Hardie et al., 2003), each of which is encoded by two or three distinct genes (α1, α2; β1, β2; γ1, γ2, γ3) (Fig. 2). All possible combinations of isoforms appear to be able to form complexes and, along with splice variants and the use of alte ...
... of a catalytic α subunit and regulatory β and γ subunits (Hardie et al., 2003), each of which is encoded by two or three distinct genes (α1, α2; β1, β2; γ1, γ2, γ3) (Fig. 2). All possible combinations of isoforms appear to be able to form complexes and, along with splice variants and the use of alte ...
Promiscuity of the Euonymus Carbohydrate-Binding Domain
... Lectins are carbohydrate-binding proteins that recognize and bind well-defined simple sugars or more complex carbohydrates in a reversible way. These carbohydrate-binding domains are widespread but have been studied most intensively within the plant kingdom. Plant lectins are a very diverse group of ...
... Lectins are carbohydrate-binding proteins that recognize and bind well-defined simple sugars or more complex carbohydrates in a reversible way. These carbohydrate-binding domains are widespread but have been studied most intensively within the plant kingdom. Plant lectins are a very diverse group of ...
The Interaction between Dietary Protein and Bone Health
... 50-89 with previous hip fractures and matched them with controls of the same age and sex in a case-control study. The 2501 subjects’ diets were assessed using a picturesort food frequency questionnaire. Protein intake was stratified into quartiles and logistic regression was applied controlling for ...
... 50-89 with previous hip fractures and matched them with controls of the same age and sex in a case-control study. The 2501 subjects’ diets were assessed using a picturesort food frequency questionnaire. Protein intake was stratified into quartiles and logistic regression was applied controlling for ...
Merlin and Expanded interact - Development
... 1316 B. M. McCartney and others the actin cytoskeleton through an N-terminal binding region (Xu and Gutmann, 1998). Consistent with this observation, overexpression of Merlin in vertebrate cultured cells causes transient alterations in the F-actin cytoskeleton (Gutmann et al., 1999). Work from seve ...
... 1316 B. M. McCartney and others the actin cytoskeleton through an N-terminal binding region (Xu and Gutmann, 1998). Consistent with this observation, overexpression of Merlin in vertebrate cultured cells causes transient alterations in the F-actin cytoskeleton (Gutmann et al., 1999). Work from seve ...