Purified dextransucrase from Pediococcus pentosaceus CRAG3 as
... (5%, w/v) but decreased thereafter, which might be due to substrate inhibition. This showed that dextransucrase from P. pentosaceus CRAG3 did not follow classical Michaelis-Menten kinetics. Similar pattern was observed with dextransucrase from P. pentosaceus11 and L. mesenteroides NRRL B-512F19. How ...
... (5%, w/v) but decreased thereafter, which might be due to substrate inhibition. This showed that dextransucrase from P. pentosaceus CRAG3 did not follow classical Michaelis-Menten kinetics. Similar pattern was observed with dextransucrase from P. pentosaceus11 and L. mesenteroides NRRL B-512F19. How ...
Small molecules that regulate lifespan
... chromosomal circular forms of rDNA known as ERCs, which can replicate during S-phase but are inefficiently segregated to daughter cells. As a result, the abundance of ERCs increases exponentially in mother cells at a rate determined by cell division, until they reach more than 1000 copies. The mecha ...
... chromosomal circular forms of rDNA known as ERCs, which can replicate during S-phase but are inefficiently segregated to daughter cells. As a result, the abundance of ERCs increases exponentially in mother cells at a rate determined by cell division, until they reach more than 1000 copies. The mecha ...
TD12 Characterization of DnaJ substrate specificity Reference
... G/E domain- contributes to stimulation of ATP hydrolysis (of DnaK) Zn binding site, amino acid ~144 C-terminal domain- amino acids 144-376, binds protein substrates Model: DnaJ first binds to unfolded protein substrates, and then hands them over to DnaK, triggering ATP hydrolysis as it delivers subs ...
... G/E domain- contributes to stimulation of ATP hydrolysis (of DnaK) Zn binding site, amino acid ~144 C-terminal domain- amino acids 144-376, binds protein substrates Model: DnaJ first binds to unfolded protein substrates, and then hands them over to DnaK, triggering ATP hydrolysis as it delivers subs ...
Nucleus-Encoded Genes for Plastid
... Genes encoding putative plastid-targeted proteins were identified from a pool of cDNA sequences produced from an ongoing Helicosporidium sp. (strain AT2000, originally isolated from the black fly, Simulium jonesii) EST project. Genes were identified for further characterization based on the followin ...
... Genes encoding putative plastid-targeted proteins were identified from a pool of cDNA sequences produced from an ongoing Helicosporidium sp. (strain AT2000, originally isolated from the black fly, Simulium jonesii) EST project. Genes were identified for further characterization based on the followin ...
iii. acidic and basic properties of amino acids
... A. Amino acids with nonpolar side chains • Each of these amino acids has a nonpolar side chain that does not gain or lose protons or participate in hydrogen or ionic bonds (see Figure 1.2). • The side chains of these amino acids can be thought of as “oily” or lipid-like 1. Location of nonpolar amino ...
... A. Amino acids with nonpolar side chains • Each of these amino acids has a nonpolar side chain that does not gain or lose protons or participate in hydrogen or ionic bonds (see Figure 1.2). • The side chains of these amino acids can be thought of as “oily” or lipid-like 1. Location of nonpolar amino ...
Nutrition, Anabolism, and the Wound Healing Process: An Overview
... Adequate protein intake to meet the demands ...
... Adequate protein intake to meet the demands ...
Virulence factors of Enterococcus spp. presented in food
... humans and farmed animals and are an integral part of commensal bacteria included in the physiological microbiota (Hammerum, 2012; Lebreton, Willems, Gilmore, 2014). Studies on the ecology and epidemiology of Enterococcus spp. indicate that along with feces these bacteria are entered into the enviro ...
... humans and farmed animals and are an integral part of commensal bacteria included in the physiological microbiota (Hammerum, 2012; Lebreton, Willems, Gilmore, 2014). Studies on the ecology and epidemiology of Enterococcus spp. indicate that along with feces these bacteria are entered into the enviro ...
Adv. Protein Chem. Struct. Biol.
... performing its action. Time-resolved Laue diffraction, intermediate trapping, time-resolved wide-angle X-ray scattering, and time-resolved X-ray absorption are the most common of them. Time-resolved Laue diffraction (Schottea et al., 2004) provides conformational snapshots of the whole protein in hi ...
... performing its action. Time-resolved Laue diffraction, intermediate trapping, time-resolved wide-angle X-ray scattering, and time-resolved X-ray absorption are the most common of them. Time-resolved Laue diffraction (Schottea et al., 2004) provides conformational snapshots of the whole protein in hi ...
Report Organelles in Blastocystis that Blur the
... found among the ESTs, three of which are likely organelle encoded. Thus, including the two subunits encoded by the mitochondrial genome fragments, we have identified a total of 11 subunits out of the 42 described for human complex I [14]: six subunits involved in electron transfer from NADH to quino ...
... found among the ESTs, three of which are likely organelle encoded. Thus, including the two subunits encoded by the mitochondrial genome fragments, we have identified a total of 11 subunits out of the 42 described for human complex I [14]: six subunits involved in electron transfer from NADH to quino ...
Construction of the yeast whole-cell Rhizopus oryzae lipase
... codon-optimized pro-ROL gene has been constructed. A sequence comparison between the codon-optimized sequence and wild-type one was shown in Tables 1 and 2. Compared to the non-optimized sequence, the fully codon-optimized gene had 217 nucleotide variations in all 1 098 nucleotides, resulting in 178 ...
... codon-optimized pro-ROL gene has been constructed. A sequence comparison between the codon-optimized sequence and wild-type one was shown in Tables 1 and 2. Compared to the non-optimized sequence, the fully codon-optimized gene had 217 nucleotide variations in all 1 098 nucleotides, resulting in 178 ...
Multiple Lines of Evidence Localize Signaling
... novel, more than doubling the number of confirmed outer membrane proteins in plant mitochondria and suggesting novel functions for the plant outer mitochondrial membrane. The novel components identified included proteins that affected mitochondrial morphology and/or segregation, a protein that sugge ...
... novel, more than doubling the number of confirmed outer membrane proteins in plant mitochondria and suggesting novel functions for the plant outer mitochondrial membrane. The novel components identified included proteins that affected mitochondrial morphology and/or segregation, a protein that sugge ...
Quantitative Proteomics of Transporter Expression in Brain Capillary
... absence of Bcrp in the Bcrp1(⫺/⫺) mice did not significantly influence the brain distribution of drugs that were shown to be good Bcrp substrates in vitro (Kodaira et al., 2010; Agarwal et al., 2011b). These findings have led to several questions about the predictive validity of the transgenic mouse ...
... absence of Bcrp in the Bcrp1(⫺/⫺) mice did not significantly influence the brain distribution of drugs that were shown to be good Bcrp substrates in vitro (Kodaira et al., 2010; Agarwal et al., 2011b). These findings have led to several questions about the predictive validity of the transgenic mouse ...
Fatty acid and phospholipid metabolism in prokaryotes
... identified and characterized in E. coli (Table 1) were identified in other bacteria, thus these genes can easily be cloned and their properties compared to those of E. coli. The pathways, especially fatty acid biosynthesis, are generally highly conserved amongst bacteria. Coupled with recent finding ...
... identified and characterized in E. coli (Table 1) were identified in other bacteria, thus these genes can easily be cloned and their properties compared to those of E. coli. The pathways, especially fatty acid biosynthesis, are generally highly conserved amongst bacteria. Coupled with recent finding ...
Proteasome inhibition induces reversible impairments in protein
... For all determinations of statistical significance, a Student’s t test was used, with P values below 0.05 considered statistically significant. ...
... For all determinations of statistical significance, a Student’s t test was used, with P values below 0.05 considered statistically significant. ...
PDF hosted at the Radboud Repository of the Radboud University
... hgpB and gene locus R2866_1095—R2866_1101 was not involved in complement resistance because deletion did not alter the complement-sensitive phenotype of the ⌬0112 mutant (Fig. 2C). To confirm the importance of this gene for complement resistance in other NTHi strains, we also deleted the R2866_0112h ...
... hgpB and gene locus R2866_1095—R2866_1101 was not involved in complement resistance because deletion did not alter the complement-sensitive phenotype of the ⌬0112 mutant (Fig. 2C). To confirm the importance of this gene for complement resistance in other NTHi strains, we also deleted the R2866_0112h ...
Processing of 20S prerRNA to 18S ribosomal RNA in yeast requires
... the inducible GAL10 promoter. The 5¢ end of this construct was also tagged with two protein A epitopes (see Supplementary data for the description of this construction). Transcription driven by the GAL10 promoter allows high expression of the gene in culture media that contain galactose (YPG, YNB Ga ...
... the inducible GAL10 promoter. The 5¢ end of this construct was also tagged with two protein A epitopes (see Supplementary data for the description of this construction). Transcription driven by the GAL10 promoter allows high expression of the gene in culture media that contain galactose (YPG, YNB Ga ...
1 - Universitas Brawijaya
... Interactive folding of several polypeptide chains together to form a “single” functional protein Functional proteins also might incorporate minerals or other nonprotein components ...
... Interactive folding of several polypeptide chains together to form a “single” functional protein Functional proteins also might incorporate minerals or other nonprotein components ...
Alfred G. Gilman - Nobel Lecture
... Rodbell, Birnbaumer, and their colleagues made the surprising discovery that one regulatory ligand (the receptor agonist) was not sufficient to activate adenylyl cyclase. A hormone could not activate the enzyme unless guanosine triphosphate (GTP) was also present {8}. This crucial observation had be ...
... Rodbell, Birnbaumer, and their colleagues made the surprising discovery that one regulatory ligand (the receptor agonist) was not sufficient to activate adenylyl cyclase. A hormone could not activate the enzyme unless guanosine triphosphate (GTP) was also present {8}. This crucial observation had be ...
Small molecules that regulate lifespan: evidence for xenohormesis
... chromosomal circular forms of rDNA known as ERCs, which can replicate during S-phase but are inefficiently segregated to daughter cells. As a result, the abundance of ERCs increases exponentially in mother cells at a rate determined by cell division, until they reach more than 1000 copies. The mecha ...
... chromosomal circular forms of rDNA known as ERCs, which can replicate during S-phase but are inefficiently segregated to daughter cells. As a result, the abundance of ERCs increases exponentially in mother cells at a rate determined by cell division, until they reach more than 1000 copies. The mecha ...
Journal of Bacteriology
... A14, and A63, the pathogenic bacteria E. carotovora subsp. carotovora and E. carotovora subsp. atroseptica, and the pathogenic fungi V. dahliae and V. albo-atrum were unable to grow around the spot of inoculation of the wild-type strain WCS358. Since no or only a small inhibition zone was observed a ...
... A14, and A63, the pathogenic bacteria E. carotovora subsp. carotovora and E. carotovora subsp. atroseptica, and the pathogenic fungi V. dahliae and V. albo-atrum were unable to grow around the spot of inoculation of the wild-type strain WCS358. Since no or only a small inhibition zone was observed a ...
ref. #28 of the TIBS article
... each residue advances a fully extended chain by Ê , the expected drop in the MGD value is 3.3 A approximately 4 1.5/3.3 1.8 residues, close to the observed drop of 1.4 residues. ...
... each residue advances a fully extended chain by Ê , the expected drop in the MGD value is 3.3 A approximately 4 1.5/3.3 1.8 residues, close to the observed drop of 1.4 residues. ...