Small molecules that regulate lifespan: evidence for xenohormesis
... chromosomal circular forms of rDNA known as ERCs, which can replicate during S-phase but are inefficiently segregated to daughter cells. As a result, the abundance of ERCs increases exponentially in mother cells at a rate determined by cell division, until they reach more than 1000 copies. The mecha ...
... chromosomal circular forms of rDNA known as ERCs, which can replicate during S-phase but are inefficiently segregated to daughter cells. As a result, the abundance of ERCs increases exponentially in mother cells at a rate determined by cell division, until they reach more than 1000 copies. The mecha ...
Journal of Bacteriology
... A14, and A63, the pathogenic bacteria E. carotovora subsp. carotovora and E. carotovora subsp. atroseptica, and the pathogenic fungi V. dahliae and V. albo-atrum were unable to grow around the spot of inoculation of the wild-type strain WCS358. Since no or only a small inhibition zone was observed a ...
... A14, and A63, the pathogenic bacteria E. carotovora subsp. carotovora and E. carotovora subsp. atroseptica, and the pathogenic fungi V. dahliae and V. albo-atrum were unable to grow around the spot of inoculation of the wild-type strain WCS358. Since no or only a small inhibition zone was observed a ...
Products for Solid State NMR - Sigma
... short-range order. In the next stage, 15N spectra and, in particular, (15N,13C) 2D data further report on molecular order and 1H bonding. In such correlation experiments, polarization transfer can either involve through-space and through-bond interactions. The choice which polarization transfer sche ...
... short-range order. In the next stage, 15N spectra and, in particular, (15N,13C) 2D data further report on molecular order and 1H bonding. In such correlation experiments, polarization transfer can either involve through-space and through-bond interactions. The choice which polarization transfer sche ...
Isolation and Expression Pattern of Human Unc-33
... 5-bromo-4-chloro-3-indolylphosphate (Roche) in AP buffer. Immunostaining with monoclonal Rip antibody, an oligodendrocyte marker (Friedman et al., 1989), was then used to visualize oligodendrocytes. The controls performed consisted of oligodendrocytes incubated in culture medium without recombinant ...
... 5-bromo-4-chloro-3-indolylphosphate (Roche) in AP buffer. Immunostaining with monoclonal Rip antibody, an oligodendrocyte marker (Friedman et al., 1989), was then used to visualize oligodendrocytes. The controls performed consisted of oligodendrocytes incubated in culture medium without recombinant ...
A Multidrug Resistance–Associated Protein Involved in
... A complete survey of the expression of the available maize MRPs (Table 1) showed that five maize MRPs are expressed in multiple tissues. Of these, ZmMrp3, ZmMrp5, ZmMrp6, and ZmMrp7 are expressed in all of the tissues that synthesize anthocyanin. As noted above, however, only ZmMrp3 shows any change ...
... A complete survey of the expression of the available maize MRPs (Table 1) showed that five maize MRPs are expressed in multiple tissues. Of these, ZmMrp3, ZmMrp5, ZmMrp6, and ZmMrp7 are expressed in all of the tissues that synthesize anthocyanin. As noted above, however, only ZmMrp3 shows any change ...
Role of the Sodium-Dependent Phosphate Cotransporters and
... the uptake of uranium by LLC-PK1 cells was assessed using culture conditions and drugs that are well known to alter absorptive endocytosis. Maintaining the cells in culture at 4C is known to be the most effective and noninvasive method of inhibiting various transport systems by modulating the membr ...
... the uptake of uranium by LLC-PK1 cells was assessed using culture conditions and drugs that are well known to alter absorptive endocytosis. Maintaining the cells in culture at 4C is known to be the most effective and noninvasive method of inhibiting various transport systems by modulating the membr ...
Nitrate Reductases: Structure, Functions, and Effect of Stress Factors
... been supposed recently that only high-potential clusters are able, for thermodynamic reasons, to transfer electrons, whereas the role of the other two clusters is still unknown. Subunit α (104-150 kD) coded by the narG gene contains the [4Fe-4S] cluster and molybdenum cofactor, just on which the nit ...
... been supposed recently that only high-potential clusters are able, for thermodynamic reasons, to transfer electrons, whereas the role of the other two clusters is still unknown. Subunit α (104-150 kD) coded by the narG gene contains the [4Fe-4S] cluster and molybdenum cofactor, just on which the nit ...
Lipid peroxidation modifies the assembly of biological membranes
... Lipid peroxidation and formation of reactive oxygen species are important chemical reactions that use oxygen and occur in cell membranes. The diffusion of oxygen into synthetic membranes prepared with phosphatidylcholine and cholesterol have been investigated by Subczynski et al. (1991). Oxygen perm ...
... Lipid peroxidation and formation of reactive oxygen species are important chemical reactions that use oxygen and occur in cell membranes. The diffusion of oxygen into synthetic membranes prepared with phosphatidylcholine and cholesterol have been investigated by Subczynski et al. (1991). Oxygen perm ...
The travels of mRNAs through all cells large and small
... nantly in the mechanism of mRNA localization in oocytes, whereas somatic cells such as fibroblasts used microfilaments for mRNA localization (26). For example, bcd RNA localization in Drosophila oocytes and Vg1 RNA localization in Xenopus oocytes could be disrupted by microtubule-depolymerizing drug ...
... nantly in the mechanism of mRNA localization in oocytes, whereas somatic cells such as fibroblasts used microfilaments for mRNA localization (26). For example, bcd RNA localization in Drosophila oocytes and Vg1 RNA localization in Xenopus oocytes could be disrupted by microtubule-depolymerizing drug ...
Chao, Elizabeth: Critical Analysis of secondary Structure Prediction Algorithms
... Secondary structure prediction using information theory. Consideration of residue pairs. The GOR method uses information theory to formulate the influence of local sequence upon the conformation of a given residue. However, the existing database does not allow the evaluation of parameters required f ...
... Secondary structure prediction using information theory. Consideration of residue pairs. The GOR method uses information theory to formulate the influence of local sequence upon the conformation of a given residue. However, the existing database does not allow the evaluation of parameters required f ...
An important role for glutathione and y
... transporter. The protein is a member of the ATPbinding cassette (ABC) family, has similarity to human multidrug resistance protein and was found to confer cadmium resistance on the yeast (Szczypka et al., 1994). Nothing is known about a possible relationship between the YCFl protein and the putative ...
... transporter. The protein is a member of the ATPbinding cassette (ABC) family, has similarity to human multidrug resistance protein and was found to confer cadmium resistance on the yeast (Szczypka et al., 1994). Nothing is known about a possible relationship between the YCFl protein and the putative ...
Uptake of Dissolved Organic Selenides by Marine Phytoplankton
... are known to have Se requirements (Price et al. 1987; Harrison et al. 1988; Doblin et al. 1999), targeted uptake of dissolved organic Se-containing compounds may occur where inorganic Se concentrations are limiting. Although organic Se in open ocean waters is thought to be relatively refractory (Cut ...
... are known to have Se requirements (Price et al. 1987; Harrison et al. 1988; Doblin et al. 1999), targeted uptake of dissolved organic Se-containing compounds may occur where inorganic Se concentrations are limiting. Although organic Se in open ocean waters is thought to be relatively refractory (Cut ...
Journal of Biological Chemistry
... as a prototype for the divalent soft metal transporting ATPases. Previously, we showed that ZntA confers resistance to Pb(II), Zn(II), and Cd(II) in E. coli by ATP-dependent efflux of these metal ions (12, 13). In this study, we report the purification of ZntA and the characterization of its soft me ...
... as a prototype for the divalent soft metal transporting ATPases. Previously, we showed that ZntA confers resistance to Pb(II), Zn(II), and Cd(II) in E. coli by ATP-dependent efflux of these metal ions (12, 13). In this study, we report the purification of ZntA and the characterization of its soft me ...
Cardosin A Molecular Determinants and Biosynthetic Pathways
... protein storage vacuoles and lytic vacuoles in the flowers and in protein bodies in seeds of the native plant cardoon. Cardosin A has been first isolated almost two decades ago and has been extensively characterized since, both in terms of distribution within the tissues and of enzyme biochemistry. ...
... protein storage vacuoles and lytic vacuoles in the flowers and in protein bodies in seeds of the native plant cardoon. Cardosin A has been first isolated almost two decades ago and has been extensively characterized since, both in terms of distribution within the tissues and of enzyme biochemistry. ...
Extreme hyperinsulinemia unmasks insulin`s effect to stimulate
... 1). During the basal period, the venous plasma insulin concentration averaged between 7 and 9 µU/ml (Table 1). During the experimental period, forearm concentrations rose nearly 1,000-fold (average 7,100–11,300 µU/ ml) and remained elevated for 4 h (Table 1). Euglycemia was maintained throughout and ...
... 1). During the basal period, the venous plasma insulin concentration averaged between 7 and 9 µU/ml (Table 1). During the experimental period, forearm concentrations rose nearly 1,000-fold (average 7,100–11,300 µU/ ml) and remained elevated for 4 h (Table 1). Euglycemia was maintained throughout and ...
Effects of Macromolecular Crowding on Protein Folding
... different macromolecules that together occupy up to 40% of its total volume. This large number of macromolecules restricts the space available to each individual molecule, which has been termed macromolecular crowding. Macromolecular crowding generates excluded volume effects and also increases the ...
... different macromolecules that together occupy up to 40% of its total volume. This large number of macromolecules restricts the space available to each individual molecule, which has been termed macromolecular crowding. Macromolecular crowding generates excluded volume effects and also increases the ...
MPN+, a putative catalytic motif found in a subset of MPN domain
... In order to gain insight into the functions and evolutionary history of MPN domain proteins such as Rpn11, we performed extended database searches using the general- ...
... In order to gain insight into the functions and evolutionary history of MPN domain proteins such as Rpn11, we performed extended database searches using the general- ...
1-3 flagellum - Instituto de Higiene
... Alternatively, the acyl modifications on some proteins such as FCaBP could cause partitioning into the unique lipid environment of the flagellar membrane. Furthermore, other proteins like ISO2 are not present in the flagellar membrane and could be either actively excluded from this compartment or re ...
... Alternatively, the acyl modifications on some proteins such as FCaBP could cause partitioning into the unique lipid environment of the flagellar membrane. Furthermore, other proteins like ISO2 are not present in the flagellar membrane and could be either actively excluded from this compartment or re ...
Physiological implications of the regulation of vacuolar
... Vacuolar H+-ATPase is a large multi-subunit protein that mediates ATP-driven vectorial H+ transport across the membranes. It is widely distributed and present in virtually all eukaryotic cells in intracellular membranes or in the plasma membrane of specialized cells. In subcellular organelles, ATPas ...
... Vacuolar H+-ATPase is a large multi-subunit protein that mediates ATP-driven vectorial H+ transport across the membranes. It is widely distributed and present in virtually all eukaryotic cells in intracellular membranes or in the plasma membrane of specialized cells. In subcellular organelles, ATPas ...
Role of the leader sequence in tobacco pectin methylesterase
... belongs to PME clade Plant 1 [4]. Unprocessed proPME protein contained the 255-aa long N-terminal leader (PS) preceding the 319-aa mature PME portion (Fig. 1A, underlined sequence). The PME from Daucus carota (P83218) might be considered as the type member of this clade, since its 3D structure was r ...
... belongs to PME clade Plant 1 [4]. Unprocessed proPME protein contained the 255-aa long N-terminal leader (PS) preceding the 319-aa mature PME portion (Fig. 1A, underlined sequence). The PME from Daucus carota (P83218) might be considered as the type member of this clade, since its 3D structure was r ...
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... termed microdomains or rafts, that acquire specific functions by concentrating or excluding specific membrane proteins (341).1 Rafts are now believed to serve as platforms for various cellular events including polarized protein sorting, signal transduction, and cell adhesion (35, ...
... termed microdomains or rafts, that acquire specific functions by concentrating or excluding specific membrane proteins (341).1 Rafts are now believed to serve as platforms for various cellular events including polarized protein sorting, signal transduction, and cell adhesion (35, ...
THE DYNAMIN SUPERFAMILY: UNIVERSAL MEMBRANE
... thaliana ADL2 binds specifically to phosphatidylinositol-4-phosphate (PtdIns4P)30 and might, in fact, be a PH domain. Lipid binding does not exclude the possibility that this domain is also involved in protein–protein interactions. Given the absence of a PRD, recruitment to mitochondria probably occ ...
... thaliana ADL2 binds specifically to phosphatidylinositol-4-phosphate (PtdIns4P)30 and might, in fact, be a PH domain. Lipid binding does not exclude the possibility that this domain is also involved in protein–protein interactions. Given the absence of a PRD, recruitment to mitochondria probably occ ...
Photopolymerized Cross-Linked Polyacrylamide Gels for On
... bovine serum albumin, 66 kDa and were prepared for on-chip SDS-PAGE per instructions from the supplier (heating to 65 °C for 5 min). Note that the absolute concentration of each protein in the stock solution is unknown, and consequently, protein concentrations are quoted relative to the stock soluti ...
... bovine serum albumin, 66 kDa and were prepared for on-chip SDS-PAGE per instructions from the supplier (heating to 65 °C for 5 min). Note that the absolute concentration of each protein in the stock solution is unknown, and consequently, protein concentrations are quoted relative to the stock soluti ...
Communications between Mitochondria, the Nucleus, Vacuoles
... 2013; Schroeder and Shadel, 2014). The resulting inactivation of Rph1 establishes an anti-aging cellular pattern because it allows to attenuate the Rph1-dependent transcription of subtelomeric chromatin regions in the nucleus, thereby lessening the extent of telomeric DNA damage (Mirisola and Longo, ...
... 2013; Schroeder and Shadel, 2014). The resulting inactivation of Rph1 establishes an anti-aging cellular pattern because it allows to attenuate the Rph1-dependent transcription of subtelomeric chromatin regions in the nucleus, thereby lessening the extent of telomeric DNA damage (Mirisola and Longo, ...