Studies on legume receptors for Nod and Myc symbiotic signals
... large number of plant viruses and other pathogens such as bacteria, oomycetes and fungi and thus is widely used in plant virology and for host–pathogen interaction studies. For the reason that its large leaves can be easily transformed with high efficiency with Agrobacterium tumefaciens, N. benthami ...
... large number of plant viruses and other pathogens such as bacteria, oomycetes and fungi and thus is widely used in plant virology and for host–pathogen interaction studies. For the reason that its large leaves can be easily transformed with high efficiency with Agrobacterium tumefaciens, N. benthami ...
DNA double-strand break repair by homologous recombination
... is required for virtually all HR events, it stands alone. The uniqueness of the RAD52 gene product is well established by the IR sensitivity of the rad52 mutant: the rad52 single mutant is as IR sensitive as a double mutant of rad52 combined with a mutation in one of the other RAD genes [54]. The ra ...
... is required for virtually all HR events, it stands alone. The uniqueness of the RAD52 gene product is well established by the IR sensitivity of the rad52 mutant: the rad52 single mutant is as IR sensitive as a double mutant of rad52 combined with a mutation in one of the other RAD genes [54]. The ra ...
A Personal Account of Chaperonin History
... but which I no longer consider very useful. We were lucky on two counts. First, we arbitrarily chose conditions for refolding that were non-permissive; i.e. no reconstitution of enzymatic activity occurred in the absence of the complete system (GroEL, GroES, and MgATP). Second, there was a fourth co ...
... but which I no longer consider very useful. We were lucky on two counts. First, we arbitrarily chose conditions for refolding that were non-permissive; i.e. no reconstitution of enzymatic activity occurred in the absence of the complete system (GroEL, GroES, and MgATP). Second, there was a fourth co ...
22: Peptides, Proteins, and α
... mixture with a variety of individual amino acids and shortened peptide chains. Aminopeptidase enzymes similarly operate on the N-terminal end of a peptide and have the same limitations as carboxypeptidases. When peptide chains are too long for accurate determination of their full sequence by a metho ...
... mixture with a variety of individual amino acids and shortened peptide chains. Aminopeptidase enzymes similarly operate on the N-terminal end of a peptide and have the same limitations as carboxypeptidases. When peptide chains are too long for accurate determination of their full sequence by a metho ...
ppt - Bio 5068
... provides a means to measure the variability in atom positions between an ensemble of structures Expanded View of an “Average” Structure ...
... provides a means to measure the variability in atom positions between an ensemble of structures Expanded View of an “Average” Structure ...
CHARACTERIZATION OF THE ROLE OF PSEUDOMONAS
... effector HopAF1, a type III effector that is present in eleven of the nineteen sequenced strains of P. syringae and other plant pathogens. Although the presence of HopAF1 in multiple strains of P. syringae suggests that it plays an important role in virulence, no function has yet been associated wit ...
... effector HopAF1, a type III effector that is present in eleven of the nineteen sequenced strains of P. syringae and other plant pathogens. Although the presence of HopAF1 in multiple strains of P. syringae suggests that it plays an important role in virulence, no function has yet been associated wit ...
Cell-Free Synthesis for Analyzing the Membrane
... commercially available that have an SP6, T7, or T3 bacteriophage promoter cloned upstream of a multiple cloning site suitable for inserting the cDNA encoding the desired protein (compare Fig. 1). These bacteriophage promoters are highly specific for their RNA polymerases. Therefore, even cDNAs encod ...
... commercially available that have an SP6, T7, or T3 bacteriophage promoter cloned upstream of a multiple cloning site suitable for inserting the cDNA encoding the desired protein (compare Fig. 1). These bacteriophage promoters are highly specific for their RNA polymerases. Therefore, even cDNAs encod ...
Degradation of Organelles or Specific Organelle Components via
... Interacting Motif (AIM), which in animals is also termed Light-chain 3 Interacting Region (LIR) [27,40,48]. Indeed, ATI1 (as a representative) possesses two AIMs located on opposite sides of a predicted trans-membrane domain. The sequence of the N-terminal AIM appears closer to the AIM/LIR consensus ...
... Interacting Motif (AIM), which in animals is also termed Light-chain 3 Interacting Region (LIR) [27,40,48]. Indeed, ATI1 (as a representative) possesses two AIMs located on opposite sides of a predicted trans-membrane domain. The sequence of the N-terminal AIM appears closer to the AIM/LIR consensus ...
COMPUTATIONAL PERSPECTIVE IN THE STRUCTURAL STABILITY OF ‘ALLALPHA’ PROTEINS: THE NH...Π INTERACTIONS
... The values of S obtained for all the amino acids were plotted in Fig. 3. The percentage ratio calculated shows that Arg make the maximum contribution to this N‐H...π interaction (52 interactions in a total of 160 interactions). It might be due to the fact that the side chain of arginin ...
... The values of S obtained for all the amino acids were plotted in Fig. 3. The percentage ratio calculated shows that Arg make the maximum contribution to this N‐H...π interaction (52 interactions in a total of 160 interactions). It might be due to the fact that the side chain of arginin ...
Two Arabidopsis Genes (IPMS1 and IPMS2
... However, IPMS1 and IPMS2 differ in their Km for acetyl-CoA (45 mM and 16 mM, respectively) and apparent quaternary structure (dimer and tetramer, respectively). A knockout insertion mutant for IPMS1 showed an increase in valine content but no changes in Leu content; two insertion mutants for IPMS2 d ...
... However, IPMS1 and IPMS2 differ in their Km for acetyl-CoA (45 mM and 16 mM, respectively) and apparent quaternary structure (dimer and tetramer, respectively). A knockout insertion mutant for IPMS1 showed an increase in valine content but no changes in Leu content; two insertion mutants for IPMS2 d ...
Developmental Regulation of Genes Encoding Universal Stress
... initially verified. Subsequently, sequence clusters and distinctive features of the sequences were determined. A total of twelve ligand binding sites were predicted based on alignment to the ATP-binding universal stress protein from Methanocaldococcus jannaschii. In addition, six USP sequences showe ...
... initially verified. Subsequently, sequence clusters and distinctive features of the sequences were determined. A total of twelve ligand binding sites were predicted based on alignment to the ATP-binding universal stress protein from Methanocaldococcus jannaschii. In addition, six USP sequences showe ...
Biochemistry 499
... aminoethanethiol and the product of that reaction with NHS-activated Sepharose, were high yielding, ~95%. Though there were impurities in the pooled fractions (figure 3b) and appeared to react with aminoethanethiol (figure 3c shows that all peaks were shifted to the left), they did not react with th ...
... aminoethanethiol and the product of that reaction with NHS-activated Sepharose, were high yielding, ~95%. Though there were impurities in the pooled fractions (figure 3b) and appeared to react with aminoethanethiol (figure 3c shows that all peaks were shifted to the left), they did not react with th ...
p-Glycoprotein-Mediated Transport of a Fluorescent Rapamycin
... similar to those shown to be effective for other MDR reversal agents, such as CSA and verapamil. These findings document an interaction between rapamycin and p-glycoprotein, but they do not address the question of whether the drug is a substrate for transport. In the present study we used confocal m ...
... similar to those shown to be effective for other MDR reversal agents, such as CSA and verapamil. These findings document an interaction between rapamycin and p-glycoprotein, but they do not address the question of whether the drug is a substrate for transport. In the present study we used confocal m ...
Presequence-Independent Manner Transported by the TIM23
... a result of MPV17 loss of function, respiration and other mitochondrial activities are dramatically reduced in tissues (69). Patients with MDDS display liver failure, hypoglycemia, growth retardation, and neuronal disorders within the first year of their life. The molecular functions of MPV17 and it ...
... a result of MPV17 loss of function, respiration and other mitochondrial activities are dramatically reduced in tissues (69). Patients with MDDS display liver failure, hypoglycemia, growth retardation, and neuronal disorders within the first year of their life. The molecular functions of MPV17 and it ...
Phosphate Groups Modifying Myelin Basic Proteins Are
... decay phase with a half-life of approximately 2 wk. However, radioactivity in the acid-soluble precursor pool (which always constituted at least half of the total radioactivity) decayed with a similar half-life, suggesting that the true turnover time of basic protein phosphates might be masked by co ...
... decay phase with a half-life of approximately 2 wk. However, radioactivity in the acid-soluble precursor pool (which always constituted at least half of the total radioactivity) decayed with a similar half-life, suggesting that the true turnover time of basic protein phosphates might be masked by co ...
fulltext - DiVA Portal
... Signal conduction as well as substance exchange through membranes, involving active and passive transport of ions, macromolecules and metabolites, are predominantly mediated by proteins. They are either an integral part of the membrane bilayer or are peripherally associated with it. Proteins involve ...
... Signal conduction as well as substance exchange through membranes, involving active and passive transport of ions, macromolecules and metabolites, are predominantly mediated by proteins. They are either an integral part of the membrane bilayer or are peripherally associated with it. Proteins involve ...
Small-molecule metabolism: an enzyme mosaic
... and Cyrus Chothia Escherichia coli has been a popular organism for studying metabolic pathways. In an attempt to find out more about how these pathways are constructed, the enzymes were analysed by defining their protein domains. Structural assignments and sequence comparisons were used to show that ...
... and Cyrus Chothia Escherichia coli has been a popular organism for studying metabolic pathways. In an attempt to find out more about how these pathways are constructed, the enzymes were analysed by defining their protein domains. Structural assignments and sequence comparisons were used to show that ...
The Metamorphosis of the Aleurone Protein Storage Vacuole
... smaller protein storage vacuoles (Jones and Price, 1970). Several pieces of evidence from electron microscopy studies support this idea. First, protein storage vacuoles are not separate organelles, but are linked by tonoplast connections (Jones and Price, 1970). Second, the large vacuole formed afte ...
... smaller protein storage vacuoles (Jones and Price, 1970). Several pieces of evidence from electron microscopy studies support this idea. First, protein storage vacuoles are not separate organelles, but are linked by tonoplast connections (Jones and Price, 1970). Second, the large vacuole formed afte ...
From Amino Acid to Glucosinolate Biosynthesis: Protein Sequence
... 5). The truncated Arabidopsis IPMS2 also showed no reduction in enzyme activity, contrary to what was observed for the Mt-IPMS protein, which lost 88% of its enzyme activity when truncated in the same manner (in subdomain II-a12, after Phe457), (Singh and Bhakuni, 2007). These results confirm that t ...
... 5). The truncated Arabidopsis IPMS2 also showed no reduction in enzyme activity, contrary to what was observed for the Mt-IPMS protein, which lost 88% of its enzyme activity when truncated in the same manner (in subdomain II-a12, after Phe457), (Singh and Bhakuni, 2007). These results confirm that t ...
The world of proteases Diversity and function
... Family S1 - Trypsin - Chymotrypsin - Elastase - RONOZYME® ProAct ...
... Family S1 - Trypsin - Chymotrypsin - Elastase - RONOZYME® ProAct ...
Inflammation and ER Stress Regulate Branched
... linked to BCAA and TCA cycle metabolism selectively in visceral but not in subcutaneous adipose tissue, brown fat, liver, or muscle. Using 3T3-L1 cells, TNF␣, and other proinflammatory cytokine treatments reduced the expression of the genes linked to BCAA transport and oxidation. Consistent with thi ...
... linked to BCAA and TCA cycle metabolism selectively in visceral but not in subcutaneous adipose tissue, brown fat, liver, or muscle. Using 3T3-L1 cells, TNF␣, and other proinflammatory cytokine treatments reduced the expression of the genes linked to BCAA transport and oxidation. Consistent with thi ...
Synthetic Physical Interactions Map Kinetochore
... 2008; Rodriguez-Bravo et al. 2014), and roles for SAC proteins in other cellular compartments (Iouk et al. 2002; Wan et al. 2014). Additionally, there is a precedent for checkpoint activation via kinetochore-independent clustering of the Aurora kinase (Campbell and Desai 2013). Therefore, we hypoth ...
... 2008; Rodriguez-Bravo et al. 2014), and roles for SAC proteins in other cellular compartments (Iouk et al. 2002; Wan et al. 2014). Additionally, there is a precedent for checkpoint activation via kinetochore-independent clustering of the Aurora kinase (Campbell and Desai 2013). Therefore, we hypoth ...