Functional Requirements of the Yellow Fever Virus Capsid Protein
... Technologies) containing 10% fetal bovine serum (FBS). Cells were grown at 37°C in the presence of 5% CO2. YFV stocks were generated by standard procedures from pYF23, a plasmid derived from pACNR/FLYF (a full-length cDNA clone of YFV 17D kindly provided by C. M. Rice). For plaque assays, 10-fold se ...
... Technologies) containing 10% fetal bovine serum (FBS). Cells were grown at 37°C in the presence of 5% CO2. YFV stocks were generated by standard procedures from pYF23, a plasmid derived from pACNR/FLYF (a full-length cDNA clone of YFV 17D kindly provided by C. M. Rice). For plaque assays, 10-fold se ...
Extended Detail on Checklist items
... protein or a coding element proposed translation product, justify that other peptides cannot be expected. Single-peptide detections simply have too high a chance of being some type of pernicious false positive to be sufficient for claiming an extraordinary result. Likewise, short peptides of length ...
... protein or a coding element proposed translation product, justify that other peptides cannot be expected. Single-peptide detections simply have too high a chance of being some type of pernicious false positive to be sufficient for claiming an extraordinary result. Likewise, short peptides of length ...
Detecting Species-Specific Proteins by LC/MS
... Markers for adulteration with cow milk All mammalian milks contain lactoglobulin, lactoferrin and casein proteins at high concentrations. A liquid/liquid extraction protocol was selective for the water soluble proteins lactoferrin and lactoglobulins present in the whey fraction of milk. Milk obtaine ...
... Markers for adulteration with cow milk All mammalian milks contain lactoglobulin, lactoferrin and casein proteins at high concentrations. A liquid/liquid extraction protocol was selective for the water soluble proteins lactoferrin and lactoglobulins present in the whey fraction of milk. Milk obtaine ...
The role of mutations in core protein of hepatitis B
... hepatocellular carcinoma [3]. The genome of hepatitis B virus encodes four overlapping open reading frames that are translated to viral core protein or HBc particle, the surface proteins, a reverse transcriptase (RT), and HBx [4]. The core protein is the major polypeptide of the nucleocapsid that du ...
... hepatocellular carcinoma [3]. The genome of hepatitis B virus encodes four overlapping open reading frames that are translated to viral core protein or HBc particle, the surface proteins, a reverse transcriptase (RT), and HBx [4]. The core protein is the major polypeptide of the nucleocapsid that du ...
Dissecting the function of Atg1 complex in Dictyostelium autophagy
... generates a PtdIns3P-enriched region at the level of the endoplasmic reticulum (ER) to which the complex formed by Atg2 and Atg18/WIPI is recruited. Concomitantly, Atg12 is covalently bound to Atg5, and the Atg12–Atg5 conjugate form a ternary complex with Atg16L1, which is required for the subsequen ...
... generates a PtdIns3P-enriched region at the level of the endoplasmic reticulum (ER) to which the complex formed by Atg2 and Atg18/WIPI is recruited. Concomitantly, Atg12 is covalently bound to Atg5, and the Atg12–Atg5 conjugate form a ternary complex with Atg16L1, which is required for the subsequen ...
DOCX - The Human Proteome Project
... intensity patterns. All SRM runs performed must have spiked-in heavy labeled peptides corresponding to the putative identifications. Annotated chromatograms must be provided in the supplementary material for the manuscript. Remember that solid peptide sequence evidence does not alter the uncertainti ...
... intensity patterns. All SRM runs performed must have spiked-in heavy labeled peptides corresponding to the putative identifications. Annotated chromatograms must be provided in the supplementary material for the manuscript. Remember that solid peptide sequence evidence does not alter the uncertainti ...
as a PDF
... ions or whether it has a primary role in iron absorption. In particular, DCT1 is probably not the primary transporter for copper: Belgrade rats that harbor a mutation in DCT1 are anemic, but exhibit normal copper status (17). Current evidence suggests that intestinal copper uptake is catalyzed by a ...
... ions or whether it has a primary role in iron absorption. In particular, DCT1 is probably not the primary transporter for copper: Belgrade rats that harbor a mutation in DCT1 are anemic, but exhibit normal copper status (17). Current evidence suggests that intestinal copper uptake is catalyzed by a ...
Characterization of Saccharomyces cerevisiae deficient in
... In the present study we have identified a gene required for the expression of PLD activity in yeast. Cells lacking expression of this gene show a complete lack of PLD activity in our in itro assay system. While it is possible that yeast expresses other forms of PLD that are not detected by this par ...
... In the present study we have identified a gene required for the expression of PLD activity in yeast. Cells lacking expression of this gene show a complete lack of PLD activity in our in itro assay system. While it is possible that yeast expresses other forms of PLD that are not detected by this par ...
Connection of the Mitochondrial Outer and Inner Membranes by
... Wickner, 1996; Jahn and Südhof, 1999). Fusion of mitochondrial membranes has been observed in a great variety of different cell types. These include budding yeast, filamentous fungi, mammalian cells in culture, and various plant cells (Bereiter-Hahn, 1990; Bereiter-Hahn and Vöth, 1994; Steinberg, 19 ...
... Wickner, 1996; Jahn and Südhof, 1999). Fusion of mitochondrial membranes has been observed in a great variety of different cell types. These include budding yeast, filamentous fungi, mammalian cells in culture, and various plant cells (Bereiter-Hahn, 1990; Bereiter-Hahn and Vöth, 1994; Steinberg, 19 ...
The plant endoplasmic reticulum: a cell-wide web
... prediction for the Arabidopsis reticulon gene family using TOPCONS indicates that all members have a predicted ‘W’ topology, with N- and C-termini, plus the short loop between the large hydrophobic regions, exposed to the cytosol [61]. The same topology was described experimentally for mammalian Rtn ...
... prediction for the Arabidopsis reticulon gene family using TOPCONS indicates that all members have a predicted ‘W’ topology, with N- and C-termini, plus the short loop between the large hydrophobic regions, exposed to the cytosol [61]. The same topology was described experimentally for mammalian Rtn ...
maize silage sampling and interpretation of analysis
... lower intakes and production. Feeding rumen buffer products such as Acid Buf® can help to alleviate this problem. Ammonia is by-product of protein breakdown. This value is virtually irrelevant to maize silage when compared to grass silage, as its protein content is proportionally much lower. Care sh ...
... lower intakes and production. Feeding rumen buffer products such as Acid Buf® can help to alleviate this problem. Ammonia is by-product of protein breakdown. This value is virtually irrelevant to maize silage when compared to grass silage, as its protein content is proportionally much lower. Care sh ...
GFP-labelled Rubisco and aspartate aminotransferase are present
... freely through stromules as well. Furthermore, the movement of GFP suggests that macromolecules like DNA and RNA might also traf®c. Stromules may, therefore, function as pathways for movement of metabolites and macromolecules between plastids in different parts of the cell. Hence, stromules may poss ...
... freely through stromules as well. Furthermore, the movement of GFP suggests that macromolecules like DNA and RNA might also traf®c. Stromules may, therefore, function as pathways for movement of metabolites and macromolecules between plastids in different parts of the cell. Hence, stromules may poss ...
pdf-Dokument - Universität Bonn
... thaliana genome, there are 15 proteins with ARFGAP domains (named AGD115) which are classified as ARFGAP Domain (AGD) proteins. These proteins have been highly conserved during the evolution of eukaryotes. In this thesis, the cellular role of an ARFGAP (AGD5) has been investigated. This group of pro ...
... thaliana genome, there are 15 proteins with ARFGAP domains (named AGD115) which are classified as ARFGAP Domain (AGD) proteins. These proteins have been highly conserved during the evolution of eukaryotes. In this thesis, the cellular role of an ARFGAP (AGD5) has been investigated. This group of pro ...
Experimental illumination of a fitness landscape
... contacts remains an unmet challenge. EMPIRIC fitness measurements provide a high-throughput approach to identify these important interactions experimentally and, hence, a route to develop and train predictive algorithms with improved accuracy. Most of the other positions analyzed exhibit a preference ...
... contacts remains an unmet challenge. EMPIRIC fitness measurements provide a high-throughput approach to identify these important interactions experimentally and, hence, a route to develop and train predictive algorithms with improved accuracy. Most of the other positions analyzed exhibit a preference ...
access full article - Caister Academic Press
... shown), confirming that the hemolytic activity is encoded in the orf1 region. The location of orf1 adjacent to the vio cluster suggests the interesting possibility that the DNA insert in pVIO4 may be derived from a pathogenicity island, a discrete segment of DNA that encodes virulence traits and oft ...
... shown), confirming that the hemolytic activity is encoded in the orf1 region. The location of orf1 adjacent to the vio cluster suggests the interesting possibility that the DNA insert in pVIO4 may be derived from a pathogenicity island, a discrete segment of DNA that encodes virulence traits and oft ...
PDF + SI - Journal of Cell Science
... Fig. 1. Distribution of retromer components across eukaryotic lineages. Most retromer components are conserved across eukaryotes, except Vps17, which is restricted to opisthokonts. Vps5 and Vps10 are frequently lost. The factors are named using S. cerevisiae nomenclature. Large taxon groupings are c ...
... Fig. 1. Distribution of retromer components across eukaryotic lineages. Most retromer components are conserved across eukaryotes, except Vps17, which is restricted to opisthokonts. Vps5 and Vps10 are frequently lost. The factors are named using S. cerevisiae nomenclature. Large taxon groupings are c ...
- Wiley Online Library
... 2007). The E. coli MreB cytoskeletal ring was shown to contain also the MreB-interacting proteins MreC, MreD, Pbp2, and RodA proteins. Whereas the MreB, MreC, MreD, and RodA were each able to independently assemble into the cytoskeletal ring, incorporation of Pbp2 into the ring required MreC (Vats e ...
... 2007). The E. coli MreB cytoskeletal ring was shown to contain also the MreB-interacting proteins MreC, MreD, Pbp2, and RodA proteins. Whereas the MreB, MreC, MreD, and RodA were each able to independently assemble into the cytoskeletal ring, incorporation of Pbp2 into the ring required MreC (Vats e ...
Plasma Amino Acid Response to Graded Levels of Escape Protein
... There was a linear (P > .001) and quadratic (P< .17) decrease in plasma concentration of lysine with an increase in supply of DDG. A negative response to supplementation may indicate that increased protein synthesis resulting from supplementation utilized more lysine than was supplied. There was a b ...
... There was a linear (P > .001) and quadratic (P< .17) decrease in plasma concentration of lysine with an increase in supply of DDG. A negative response to supplementation may indicate that increased protein synthesis resulting from supplementation utilized more lysine than was supplied. There was a b ...
Stockholm University
... However, only a few attempts have been made to predict the accessibility of membrane proteins [9,13-15]. To our knowledge all existing methods have been specialized to predict the exposure within the membrane. Therefore, these methods require an initial prediction step to determine the exact locatio ...
... However, only a few attempts have been made to predict the accessibility of membrane proteins [9,13-15]. To our knowledge all existing methods have been specialized to predict the exposure within the membrane. Therefore, these methods require an initial prediction step to determine the exact locatio ...
Investigation of the interactions between MreB, the
... allosteric communication, also differs from GroEL. Furthermore, TRiC is larger than GroEL with a broader opening of the chaperonin chamber. (5) Despite these differences, TRiC and GroEL are in many ways similar. As mentioned in the introduction to this report, studies on actin by Villebeck et al. (5 ...
... allosteric communication, also differs from GroEL. Furthermore, TRiC is larger than GroEL with a broader opening of the chaperonin chamber. (5) Despite these differences, TRiC and GroEL are in many ways similar. As mentioned in the introduction to this report, studies on actin by Villebeck et al. (5 ...
Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and
... Smc3p, maintains sister chromatid arm cohesion from S phase until the onset of anaphase (for review, see Koshland and Guacci 2000; Nasmyth et al. 2000; Amon 2001). Cohesin proteins are more densely distributed at the centromere and coimmunoprecipitate with CEN DNA (Blat and Kleckner 1999; Megee et a ...
... Smc3p, maintains sister chromatid arm cohesion from S phase until the onset of anaphase (for review, see Koshland and Guacci 2000; Nasmyth et al. 2000; Amon 2001). Cohesin proteins are more densely distributed at the centromere and coimmunoprecipitate with CEN DNA (Blat and Kleckner 1999; Megee et a ...
Oxidized Amino Acids in Lens Protein with Age
... fluorescence that accumulates in human lens proteins with age, there is no information available concerning changes in amounts of DT or other oxidation products of aromatic amino acids in the noncataractous, aging human lens. Because of the prior evidence for the presence of DT in lens and the incre ...
... fluorescence that accumulates in human lens proteins with age, there is no information available concerning changes in amounts of DT or other oxidation products of aromatic amino acids in the noncataractous, aging human lens. Because of the prior evidence for the presence of DT in lens and the incre ...