Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and
... Smc3p, maintains sister chromatid arm cohesion from S phase until the onset of anaphase (for review, see Koshland and Guacci 2000; Nasmyth et al. 2000; Amon 2001). Cohesin proteins are more densely distributed at the centromere and coimmunoprecipitate with CEN DNA (Blat and Kleckner 1999; Megee et a ...
... Smc3p, maintains sister chromatid arm cohesion from S phase until the onset of anaphase (for review, see Koshland and Guacci 2000; Nasmyth et al. 2000; Amon 2001). Cohesin proteins are more densely distributed at the centromere and coimmunoprecipitate with CEN DNA (Blat and Kleckner 1999; Megee et a ...
Investigation of the interactions between MreB, the
... allosteric communication, also differs from GroEL. Furthermore, TRiC is larger than GroEL with a broader opening of the chaperonin chamber. (5) Despite these differences, TRiC and GroEL are in many ways similar. As mentioned in the introduction to this report, studies on actin by Villebeck et al. (5 ...
... allosteric communication, also differs from GroEL. Furthermore, TRiC is larger than GroEL with a broader opening of the chaperonin chamber. (5) Despite these differences, TRiC and GroEL are in many ways similar. As mentioned in the introduction to this report, studies on actin by Villebeck et al. (5 ...
Hutational analysis of the influenza virus A/Victoria/3/75 PA protein
... that the N terminus is not absolutely required for such interaction. Since none of the 12 C-terminal and internal PA deletion mutants showed binding to PB1 it might be concluded that the entire C-terminal three quarters of PA are involved in this association. PBI may be bound at several points and/o ...
... that the N terminus is not absolutely required for such interaction. Since none of the 12 C-terminal and internal PA deletion mutants showed binding to PB1 it might be concluded that the entire C-terminal three quarters of PA are involved in this association. PBI may be bound at several points and/o ...
ASM book 1.8.7.20 vgv - BioQUEST Curriculum Consortium
... distinct domains. Some bacteria-like organisms look like normal bacteria but may have had a distinct phylogenetic history. Consequently, these bacteria-like organisms may comprise a distinct domain, given the name Archaebacteria, or more simply, Archaea. The name reflects an untested conjecture abou ...
... distinct domains. Some bacteria-like organisms look like normal bacteria but may have had a distinct phylogenetic history. Consequently, these bacteria-like organisms may comprise a distinct domain, given the name Archaebacteria, or more simply, Archaea. The name reflects an untested conjecture abou ...
Journal of Medical Microbiology
... N-terminal amino acid sequence of the 71.5-kDa HSBP did not show homology to any other heparin-binding protein, nor to known proteins of H. pylori, whereas the 66.2-kDa HSBP showed a high homology to an Escherichia coli chaperon protein and equine haemoglobin. A third HSBP was isolated from an outer ...
... N-terminal amino acid sequence of the 71.5-kDa HSBP did not show homology to any other heparin-binding protein, nor to known proteins of H. pylori, whereas the 66.2-kDa HSBP showed a high homology to an Escherichia coli chaperon protein and equine haemoglobin. A third HSBP was isolated from an outer ...
Protein Sorting and Transport - The Endoplasmic Reticulum, Golgi
... cytosol. Ribosomes engaged in the synthesis of proteins that are destined for secretion are then targeted to the endoplasmic reticulum by a signal sequence at the amino terminus of the growing polypeptide chain. These signal sequences are short stretches of hydrophobic amino acids that are cleaved f ...
... cytosol. Ribosomes engaged in the synthesis of proteins that are destined for secretion are then targeted to the endoplasmic reticulum by a signal sequence at the amino terminus of the growing polypeptide chain. These signal sequences are short stretches of hydrophobic amino acids that are cleaved f ...
Alpha-COPI Coatomer Protein Is Required for Rough Endoplasmic
... RNAi to functionally identify midgut epithelial cell proteins that contribute to RER whorl formation. Methodology/Principal Findings: Adult female Ae. aegypti mosquitoes were maintained on sugar alone (unfed), or fed an amino acid meal, and then midgut epithelial cells were analyzed by electron micr ...
... RNAi to functionally identify midgut epithelial cell proteins that contribute to RER whorl formation. Methodology/Principal Findings: Adult female Ae. aegypti mosquitoes were maintained on sugar alone (unfed), or fed an amino acid meal, and then midgut epithelial cells were analyzed by electron micr ...
ARTÍCULOS
... characterize the interaction between H-NS and Hha. We show that the presence of DNA in preparations of copurified His-Hha and H-NS is not directly implicated in the interaction between the proteins. The precise molecular mass of the H-NS protein retained by Hha, obtained by mass spectrometry analysis ...
... characterize the interaction between H-NS and Hha. We show that the presence of DNA in preparations of copurified His-Hha and H-NS is not directly implicated in the interaction between the proteins. The precise molecular mass of the H-NS protein retained by Hha, obtained by mass spectrometry analysis ...
Dirty Business - American Chemical Society
... phosphorus, calcium and magnesium), fats, vitamin and glucose. The albumin makes up about two-thirds of the weight of the egg’s contents, not including the shell. A little more than 50% of the egg’s protein is contained in the albumin. There is no cholesterol in the albumin. So, let’s consider the b ...
... phosphorus, calcium and magnesium), fats, vitamin and glucose. The albumin makes up about two-thirds of the weight of the egg’s contents, not including the shell. A little more than 50% of the egg’s protein is contained in the albumin. There is no cholesterol in the albumin. So, let’s consider the b ...
Dual Location of the Mitochondrial Preprotein
... Tim23-3 proteins, and no cross-reactivity was observed (see Supplemental Figure 1 online). An antibody raised against Tim50 also detected the upper band of Tim17:23 complex, but not complex I (Figure 2B). To confirm further the dual locations of both Tim23-2 and B14.7, in vitro protein uptake assays ...
... Tim23-3 proteins, and no cross-reactivity was observed (see Supplemental Figure 1 online). An antibody raised against Tim50 also detected the upper band of Tim17:23 complex, but not complex I (Figure 2B). To confirm further the dual locations of both Tim23-2 and B14.7, in vitro protein uptake assays ...
Imaging ER-to-Golgi transport: towards a
... microscope; 3D stacks covering 59 different image planes were acquired every 15 minutes. In interphase, ERES are visible as fluorescent dots throughout the cell and accumulated in the juxtanuclear area (i). Before metaphase, the number and intensity of ERES is significantly reduced (ii); in metaphas ...
... microscope; 3D stacks covering 59 different image planes were acquired every 15 minutes. In interphase, ERES are visible as fluorescent dots throughout the cell and accumulated in the juxtanuclear area (i). Before metaphase, the number and intensity of ERES is significantly reduced (ii); in metaphas ...
Chapter 7
... cell to gain or lose water • Isotonic solution: Solute concentration is the same as that inside the cell; no net water movement across the plasma membrane • Hypertonic solution: Solute concentration is greater than that inside the cell; cell loses ...
... cell to gain or lose water • Isotonic solution: Solute concentration is the same as that inside the cell; no net water movement across the plasma membrane • Hypertonic solution: Solute concentration is greater than that inside the cell; cell loses ...
REVIEW - The Journal of Cell Biology
... intermembrane space. Centrifugation of osmotically shocked chloroplasts on discontinuous sucrose gradients yields an envelope fraction substantially free of contamination by other chloroplast components (38, 112, 113), but it does not allow the separation of the inner and outer envelope membranes. T ...
... intermembrane space. Centrifugation of osmotically shocked chloroplasts on discontinuous sucrose gradients yields an envelope fraction substantially free of contamination by other chloroplast components (38, 112, 113), but it does not allow the separation of the inner and outer envelope membranes. T ...
BIOMOLECULES: INTRODUCTION, STRUCTURE AND FUNCTION
... Keratins of skin, hair and nail; silk protein and collagens are the best examples of this class of proteins. Very often we come across proteins that can not be placed in either of these categories. They have large size and possess irregular structures with limited contents of conventional structures ...
... Keratins of skin, hair and nail; silk protein and collagens are the best examples of this class of proteins. Very often we come across proteins that can not be placed in either of these categories. They have large size and possess irregular structures with limited contents of conventional structures ...
Sequence-based predictions of membrane-protein topology, homology and insertion
... propagation of the cell and the genetic material it carries. Key players in this chemical factory of life are the proteins, catalyzing the specific conversion of substrates to products, regulating each others' concentrations, transporting molecules across barriers, and converting light into sugar, a ...
... propagation of the cell and the genetic material it carries. Key players in this chemical factory of life are the proteins, catalyzing the specific conversion of substrates to products, regulating each others' concentrations, transporting molecules across barriers, and converting light into sugar, a ...
Viral protein targeting to the cortical endoplasmic reticulum is
... various strategies to spread from one cell to another. Animal viruses commonly use vesicular transport machineries in the se cretory and endocytosis/exocytosis pathways for entry and exit (Pelkmans et al., 2001; Sieczkarski and Whittaker, 2002; Smith and Helenius, 2004; Greber and Way, 2006). Howev ...
... various strategies to spread from one cell to another. Animal viruses commonly use vesicular transport machineries in the se cretory and endocytosis/exocytosis pathways for entry and exit (Pelkmans et al., 2001; Sieczkarski and Whittaker, 2002; Smith and Helenius, 2004; Greber and Way, 2006). Howev ...
phylogenetic analysis of pdv movement protein compared to
... The results obtained in the present study concerning a comparison of the MP and RBD MP amino acid sequences among Prune dwarf virus demonstrate the existence of some level of differentiation. The MP amino acid sequences of MP differed by 4.55% on average, i.e. not more than 27 aa. These results are ...
... The results obtained in the present study concerning a comparison of the MP and RBD MP amino acid sequences among Prune dwarf virus demonstrate the existence of some level of differentiation. The MP amino acid sequences of MP differed by 4.55% on average, i.e. not more than 27 aa. These results are ...
Selective Isotope-Labeling Methods for Protein Structural Studies
... One of the major contributing factors to the rapid advance of biomolecular NMR spectroscopy is the emergence of different isotope-labeling methods. Recent developments in biotechnology have made it easier and economical to introduce 13C,15N and 2H into proteins and nucleic acids. At the same time, t ...
... One of the major contributing factors to the rapid advance of biomolecular NMR spectroscopy is the emergence of different isotope-labeling methods. Recent developments in biotechnology have made it easier and economical to introduce 13C,15N and 2H into proteins and nucleic acids. At the same time, t ...
Structure-Function Analysis of the UDP-N-acetyl-D
... represent key regulatory factors in defining the repertoire of Oglycans expressed by a cell. In vitro studies have shown that some ppGaNTases (ppGaNTase-T1, -T2, and -T3) glycosylate a broad spectrum of peptides (2, 3), whereas others exhibit more restricted (ppGaNTase-T4 and -T5) or unique (ppGaNTa ...
... represent key regulatory factors in defining the repertoire of Oglycans expressed by a cell. In vitro studies have shown that some ppGaNTases (ppGaNTase-T1, -T2, and -T3) glycosylate a broad spectrum of peptides (2, 3), whereas others exhibit more restricted (ppGaNTase-T4 and -T5) or unique (ppGaNTa ...
NH 2
... - first globular protein whose structure was analysed by Xray diffraction by protein crystals. The periodic repeats characteristic of alpha helix were recognised, and the structure shown to have 70% of the polypeptide is alphahelical. - it is O2 storage site in muscle tissue. - It is also intracellu ...
... - first globular protein whose structure was analysed by Xray diffraction by protein crystals. The periodic repeats characteristic of alpha helix were recognised, and the structure shown to have 70% of the polypeptide is alphahelical. - it is O2 storage site in muscle tissue. - It is also intracellu ...
Suppression mechanisms
... Mutations that identify direct interactions between two proteins are among the most commonly desired class of suppressors. In the most simplistic scenario, an amino acid change at the interface between two proteins disrupts their interaction and a compensatory amino acid substitution in the second p ...
... Mutations that identify direct interactions between two proteins are among the most commonly desired class of suppressors. In the most simplistic scenario, an amino acid change at the interface between two proteins disrupts their interaction and a compensatory amino acid substitution in the second p ...
Protein Engineering in the Development of
... of hybrid hydrogels in response to binding events (15, 48–50). Nature is rich with diversity: There are many protein domains and peptide motifs with interesting ligand binding properties, with structures that undergo conformation changes, and that have complex functions yet to be used in responsive ...
... of hybrid hydrogels in response to binding events (15, 48–50). Nature is rich with diversity: There are many protein domains and peptide motifs with interesting ligand binding properties, with structures that undergo conformation changes, and that have complex functions yet to be used in responsive ...