Lysosomal and vacuolar sorting: not so different
... organelles via two routes: the canonical or the alternative route. In the canonical pathway, the receptors are recognized by the adaptor complexes AP1 and/or AP4 at the TGN level. This triggers vesicular targeting to the endosomes in a clathrin dependent pathway [30,34,38,47]. When they reach the la ...
... organelles via two routes: the canonical or the alternative route. In the canonical pathway, the receptors are recognized by the adaptor complexes AP1 and/or AP4 at the TGN level. This triggers vesicular targeting to the endosomes in a clathrin dependent pathway [30,34,38,47]. When they reach the la ...
Studies of focal adhesion assembly
... composed of three different domain types: Fn1, Fn2 and Fn3. High-resolution structures of many of these component domains and domain combinations have been available for some time [6]; the main remaining uncertainties are associated with how Fn forms complexes with its various partners. Fn has confo ...
... composed of three different domain types: Fn1, Fn2 and Fn3. High-resolution structures of many of these component domains and domain combinations have been available for some time [6]; the main remaining uncertainties are associated with how Fn forms complexes with its various partners. Fn has confo ...
Infectious Salmon Anaemia Virus (ISAV) RNA Binding Protein
... Abstract: Infectious salmon anaemia virus (ISAV) is an orthomyxovirus infecting salmonid fish. The virus is adapted to low temperature and has a replication optimum between 10–15 ˝ C. In this study the subcellular localization and protein interactions for the protein encoded by the largest open read ...
... Abstract: Infectious salmon anaemia virus (ISAV) is an orthomyxovirus infecting salmonid fish. The virus is adapted to low temperature and has a replication optimum between 10–15 ˝ C. In this study the subcellular localization and protein interactions for the protein encoded by the largest open read ...
Translation of an integral membrane protein in distal dendrites of
... cultured hippocampal neurons. Synthesis of the CFP–TGN-38 reporter construct could be seen in a previously photobleached dendritic region of transfected neurons less than 30 min after stimulation of the neurons with glutamate and glycine. This synthesis was sensitive to the translational inhibitor a ...
... cultured hippocampal neurons. Synthesis of the CFP–TGN-38 reporter construct could be seen in a previously photobleached dendritic region of transfected neurons less than 30 min after stimulation of the neurons with glutamate and glycine. This synthesis was sensitive to the translational inhibitor a ...
View Full PDF - Biochemical Society Transactions
... organelles via two routes: the canonical or the alternative route. In the canonical pathway, the receptors are recognized by the adaptor complexes AP1 and/or AP4 at the TGN level. This triggers vesicular targeting to the endosomes in a clathrin dependent pathway [30,34,38,47]. When they reach the la ...
... organelles via two routes: the canonical or the alternative route. In the canonical pathway, the receptors are recognized by the adaptor complexes AP1 and/or AP4 at the TGN level. This triggers vesicular targeting to the endosomes in a clathrin dependent pathway [30,34,38,47]. When they reach the la ...
Thermostable glycerol kinase from a
... solfatara at a wharf on Kodakara Island, Kagoshima, Japan (Morikawa et al., 1994). The growth temperature of this strain ranged from 65 to 100°C and the optimal temperature is 95°C. Here we report that GK from P.kodakaraensis KOD1 showed distinctive structural and enzymatic characteristics as compar ...
... solfatara at a wharf on Kodakara Island, Kagoshima, Japan (Morikawa et al., 1994). The growth temperature of this strain ranged from 65 to 100°C and the optimal temperature is 95°C. Here we report that GK from P.kodakaraensis KOD1 showed distinctive structural and enzymatic characteristics as compar ...
Chemistry 202 Amino Acids, Peptides, and
... and would be named seryl-alanyl-glycine (Ser-Ala-Gly). It is clear that when 100 or more amino acids are involved and each of these may have one of 20 different structures, the number of possible proteins is huge. Some commonly occurring peptides are antibiotics, hormones, and enzymes. Besides many ...
... and would be named seryl-alanyl-glycine (Ser-Ala-Gly). It is clear that when 100 or more amino acids are involved and each of these may have one of 20 different structures, the number of possible proteins is huge. Some commonly occurring peptides are antibiotics, hormones, and enzymes. Besides many ...
Plant serine/arginine-rich proteins and their role in pre
... adapters and recruit other components of the splicing machinery. The RRM, which confers RNA-binding specificity, binds to specific regulatory sequences in pre-mRNA, and the RS domain functions as a splicing regulator by mediating protein–protein interactions with other components of the splicing mac ...
... adapters and recruit other components of the splicing machinery. The RRM, which confers RNA-binding specificity, binds to specific regulatory sequences in pre-mRNA, and the RS domain functions as a splicing regulator by mediating protein–protein interactions with other components of the splicing mac ...
Holbert, Daniel: Detecting motifs with EMOTIF-MAKER and MASIA: A critical comparison of two tools for finding protein motifs
... undesirable results for training data that contain subsets of especially highly related sequences. For example, a hypothetical training set that contains four closely related human sequences and one distant bacterial sequence would probably have many positions at which the human sequences all agreed ...
... undesirable results for training data that contain subsets of especially highly related sequences. For example, a hypothetical training set that contains four closely related human sequences and one distant bacterial sequence would probably have many positions at which the human sequences all agreed ...
Multidrug resistance mediated by the ATP-binding
... MOAT proteins, these MRP-related transporters have been identified in a variety of species, such as the heavy metal resistance protein MRP1 in Caenorhabditis elegans,(26) the cadmium resistance factor protein YCF1 and oligomycin resistance protein YOR1/YRS1 in Saccharomyces cerevisiae,(27,28) and th ...
... MOAT proteins, these MRP-related transporters have been identified in a variety of species, such as the heavy metal resistance protein MRP1 in Caenorhabditis elegans,(26) the cadmium resistance factor protein YCF1 and oligomycin resistance protein YOR1/YRS1 in Saccharomyces cerevisiae,(27,28) and th ...
Modelling neurodegeneration in Saccharomyces
... • Chemical genetic profiling. Screening of a compound against the entire library of haploid deletion strains (generating a ‘fitness profile’) to help identify the pathway and potentially the actual protein targeted by the compound. Comparing the compound’s genetic profile with synthetic genetic leth ...
... • Chemical genetic profiling. Screening of a compound against the entire library of haploid deletion strains (generating a ‘fitness profile’) to help identify the pathway and potentially the actual protein targeted by the compound. Comparing the compound’s genetic profile with synthetic genetic leth ...
Crystal structure of yeast hexokinase Pl in complex
... the later position should be occupied by a certain type of amino acid residue). If the amino acid distribution at the former position in the alignment varies (which is reflected in a high value for DDG stat), this means that for some reason, normally related to the structure and/or function of the p ...
... the later position should be occupied by a certain type of amino acid residue). If the amino acid distribution at the former position in the alignment varies (which is reflected in a high value for DDG stat), this means that for some reason, normally related to the structure and/or function of the p ...
“Nice” plotting of proteins
... After the lengthy “introduction” to polynomials and interpolation it is about the time to ask: “Is the polynomial fit any good for protein chains”? Can we use polynomial fits to get a much smoother and better views of protein structures? Polynomial fits are sound if higher order derivatives of the “ ...
... After the lengthy “introduction” to polynomials and interpolation it is about the time to ask: “Is the polynomial fit any good for protein chains”? Can we use polynomial fits to get a much smoother and better views of protein structures? Polynomial fits are sound if higher order derivatives of the “ ...
Biochemical and Molecular Characterization of the Chicken
... consensus amino acid sequence CX2CX,6_23HX2CX2CX2CXI6_21CX2_~(C,H,D)(Sadler et al., 1992), has been identified in a number of gene products (Table I), several of which have been proposed to function as transcription factors. The LIM motif was first defined in proteins that also contain homeodomain s ...
... consensus amino acid sequence CX2CX,6_23HX2CX2CX2CXI6_21CX2_~(C,H,D)(Sadler et al., 1992), has been identified in a number of gene products (Table I), several of which have been proposed to function as transcription factors. The LIM motif was first defined in proteins that also contain homeodomain s ...
IdaPro® Milk Proteins - SupplySide Storefronts
... Proteins vs Whey Proteins In testing of three market available WPC-80 samples and three IdaPro ® MPI-85 samples, results of assays conducted by independent testing laboratories showed that the Amino Acid (AA) profiles are very similar.6 The slight differences in Essential AA’s per 100 grams of prote ...
... Proteins vs Whey Proteins In testing of three market available WPC-80 samples and three IdaPro ® MPI-85 samples, results of assays conducted by independent testing laboratories showed that the Amino Acid (AA) profiles are very similar.6 The slight differences in Essential AA’s per 100 grams of prote ...
Inhibition Systems Mechanisms and Biological Roles of
... often precipitated by perturbations to the microbiome resulting from antibiotic treatment (Bakken et al. 2011). Moreover, antibiotic treatment can increase susceptibility to influenza virus through alterations in the microbiome and its interplay with the host immune system (Ichinohe et al. 2011). Th ...
... often precipitated by perturbations to the microbiome resulting from antibiotic treatment (Bakken et al. 2011). Moreover, antibiotic treatment can increase susceptibility to influenza virus through alterations in the microbiome and its interplay with the host immune system (Ichinohe et al. 2011). Th ...
國立清華大學 - 罕見疾病基金會
... Mitochondrial targeting sequence Mostly cytosolic precursors (usually unfolded or loosely folded) of mitochondrial proteins need a mitochondrial targeting signal (MTS) to help their translocation into mitochondria. This signal will be recognized by the translocase of the outer-membrane (TOM) and the ...
... Mitochondrial targeting sequence Mostly cytosolic precursors (usually unfolded or loosely folded) of mitochondrial proteins need a mitochondrial targeting signal (MTS) to help their translocation into mitochondria. This signal will be recognized by the translocase of the outer-membrane (TOM) and the ...
Translocation Arrest by Reversible Folding of a Precursor Protein
... made use of the following observation. Import and processing of a fusion protein between a mitochondrial presequence (amino acid residues 1-22 of cytochrome oxidase subunit IV) and dihydrofolate reductase (DHFR) t was found to be inhibited after binding of the folate antagonist methotrexate to the D ...
... made use of the following observation. Import and processing of a fusion protein between a mitochondrial presequence (amino acid residues 1-22 of cytochrome oxidase subunit IV) and dihydrofolate reductase (DHFR) t was found to be inhibited after binding of the folate antagonist methotrexate to the D ...
Combinatorial mutagenesis to restrict amino acid usage in an
... without compromising their structure and function (16–21), whereas a few amino acid substitutions can exert large effects on protein structure (22) and function (23, 24). To select allowable substitutions at permissive positions, we used the growth complementation of the pyrE-deficient E. coli strai ...
... without compromising their structure and function (16–21), whereas a few amino acid substitutions can exert large effects on protein structure (22) and function (23, 24). To select allowable substitutions at permissive positions, we used the growth complementation of the pyrE-deficient E. coli strai ...
Comparison of environmental and isolate Sulfobacillus genomes
... fragments, and trained for 100 epochs using the k-batch training method, along with any leftover fragments exceeding 3 kb in length. To minimize noise, sequences in the range 1500–3000 bp were not included during training, but instead projected onto the trained weight vectors generated with the larg ...
... fragments, and trained for 100 epochs using the k-batch training method, along with any leftover fragments exceeding 3 kb in length. To minimize noise, sequences in the range 1500–3000 bp were not included during training, but instead projected onto the trained weight vectors generated with the larg ...
Methods to Make Homogenous Antibody Drug Conjugates
... therefore compete for stop codon suppression of nnAAtRNAs. Thus, full length protein production of nnAA containing antibodies can be significantly reduced in titer when compared to wild-type translation. While removal of the competing RF1 for amber suppression is therefore desirable, this is complic ...
... therefore compete for stop codon suppression of nnAAtRNAs. Thus, full length protein production of nnAA containing antibodies can be significantly reduced in titer when compared to wild-type translation. While removal of the competing RF1 for amber suppression is therefore desirable, this is complic ...
Georgatsou E. and Alexandraki D. - IMBB-FORTH in
... to Fe2+ (6, 8, 10, 20, 39). The transmembrane reductase apparently uses NADH as the intracellular electron donor (40). Following the reduction of iron, a transmembrane ferrous transporter system delivers the ferrous ions inside the cell. The two systems, the reductase and the transporter, have been ...
... to Fe2+ (6, 8, 10, 20, 39). The transmembrane reductase apparently uses NADH as the intracellular electron donor (40). Following the reduction of iron, a transmembrane ferrous transporter system delivers the ferrous ions inside the cell. The two systems, the reductase and the transporter, have been ...