implications for key virulence factors in Flavobacterium columnare
... has been shown to be virulent in fish, in which the derivative Rough and Soft types are non-virulent [18-20]. Therefore, identification of the structures and cell organisation of these virulent and non-virulent types can provide valuable information on how bacteria behave outside the host and offer ...
... has been shown to be virulent in fish, in which the derivative Rough and Soft types are non-virulent [18-20]. Therefore, identification of the structures and cell organisation of these virulent and non-virulent types can provide valuable information on how bacteria behave outside the host and offer ...
The hnRNP C Proteins Contain a Nuclear Retention Sequence That
... the cytoplasm. These shuttling hnRNP proteins include hnRNP A1, K, and E (Pifiol-Roma and Dreyfuss, 1992, 1993a; Michael et al., 1995b). While in the cytoplasm, hnRNP A1 is associated with mRNA, suggesting that shuttling hnRNP proteins accompany mRNA from the point of its emergence from the transcri ...
... the cytoplasm. These shuttling hnRNP proteins include hnRNP A1, K, and E (Pifiol-Roma and Dreyfuss, 1992, 1993a; Michael et al., 1995b). While in the cytoplasm, hnRNP A1 is associated with mRNA, suggesting that shuttling hnRNP proteins accompany mRNA from the point of its emergence from the transcri ...
The Roles of Moonlighting Proteins in Bacteria
... along with their classic neuropeptide function (Campbell and Scanes, 1995). The term “moonlighting protein” was introduced to describe a single protein that has multiple functions not due to gene fusions, splice variants or multiple proteolytic fragments (Jeffery, 2009; Huberts and van der Klei, 201 ...
... along with their classic neuropeptide function (Campbell and Scanes, 1995). The term “moonlighting protein” was introduced to describe a single protein that has multiple functions not due to gene fusions, splice variants or multiple proteolytic fragments (Jeffery, 2009; Huberts and van der Klei, 201 ...
Rapid movement of axonal neurofilaments interrupted by prolonged
... yielded conflicting results. The first study of this kind reported anterograde movement of tubulin in cultured PC12 cells 2, but subsequent studies on tubulin in PC12 cells3, cultured chick sensory neurons4 and developing neurons in grasshopper and zebrafish embryos5,6, as well as studies of tubulin ...
... yielded conflicting results. The first study of this kind reported anterograde movement of tubulin in cultured PC12 cells 2, but subsequent studies on tubulin in PC12 cells3, cultured chick sensory neurons4 and developing neurons in grasshopper and zebrafish embryos5,6, as well as studies of tubulin ...
Protein Interactions Techniques and Challenges
... potential, hydrophobocity, residue interface propensity) can affect the interaction of two molecules. ...
... potential, hydrophobocity, residue interface propensity) can affect the interaction of two molecules. ...
Multimeric Protein Structures of African Horsesickness Virus
... AHSV virion is composed of seven structural proteins organised into a double layered capsid, which encloses ten double-stranded RNA segments. The double stranded (ds) RNA genome of AHSV encodes, in addition to the seven structural proteins, at least three non-structural assembly of viral proteins in ...
... AHSV virion is composed of seven structural proteins organised into a double layered capsid, which encloses ten double-stranded RNA segments. The double stranded (ds) RNA genome of AHSV encodes, in addition to the seven structural proteins, at least three non-structural assembly of viral proteins in ...
How to obtain labeled proteins and what to do with... Marlon J Hinner and Kai Johnsson
... established method for the fluorescent labeling of small tags within cells. The specificity of biarsenical labels for their tag is not perfect; labeling and washing procedures therefore have to be carried out in presence of a competing thiol reagent to reduce background labeling. This labeling proce ...
... established method for the fluorescent labeling of small tags within cells. The specificity of biarsenical labels for their tag is not perfect; labeling and washing procedures therefore have to be carried out in presence of a competing thiol reagent to reduce background labeling. This labeling proce ...
Protein Structure Prediction
... selected from PDB and other databases such as PSST (http://pranag.physics.iisc.ernet.in/psst) and PRF (http://www.genome.ad.jp/) have been selected. • The data set has been restricted to those biologically active peptides that consist of only natural amino acids and are linear with length varying be ...
... selected from PDB and other databases such as PSST (http://pranag.physics.iisc.ernet.in/psst) and PRF (http://www.genome.ad.jp/) have been selected. • The data set has been restricted to those biologically active peptides that consist of only natural amino acids and are linear with length varying be ...
Novel Multiprotein Complexes Identified in the Hyperthermophilic
... false positives (16). The epitope tag affinity purification and tandem affinity purification methods have also been used extensively (17–21), but the tags can disrupt native proteinprotein interactions, and the methods tend to be biased toward proteins that interact with high affinity and/or protein ...
... false positives (16). The epitope tag affinity purification and tandem affinity purification methods have also been used extensively (17–21), but the tags can disrupt native proteinprotein interactions, and the methods tend to be biased toward proteins that interact with high affinity and/or protein ...
Pavel Doležal
... possessing double-membrane bound organelles such as hydrogen-producing hydrogenosomes and newly characterized organelles, the mitosomes. These organelles were suggested to evolve either from a common pro-mitochondrial endosymbiont, or they represent highly modified or reduced mitochondria. Although ...
... possessing double-membrane bound organelles such as hydrogen-producing hydrogenosomes and newly characterized organelles, the mitosomes. These organelles were suggested to evolve either from a common pro-mitochondrial endosymbiont, or they represent highly modified or reduced mitochondria. Although ...
Translocation of proteins across archaeal cytoplasmic membranes
... Computational analyses of signal sequences from the euryarchaeon Methanocaldococcus jannaschii [19] and the crenarchaeon Sulfolobus solfataricus [20] suggest that (i) these signal sequences possess a charge distribution ...
... Computational analyses of signal sequences from the euryarchaeon Methanocaldococcus jannaschii [19] and the crenarchaeon Sulfolobus solfataricus [20] suggest that (i) these signal sequences possess a charge distribution ...
Integration and topology of membrane proteins Carolina Boekel
... system that allows a quantitative assessment of membrane insertion efficiency. Our studies suggest that helices might interact with each other already during the membrane-insertion step, possibly forming helical hairpins that partition into the membrane as a single unit. Further, the insertion effic ...
... system that allows a quantitative assessment of membrane insertion efficiency. Our studies suggest that helices might interact with each other already during the membrane-insertion step, possibly forming helical hairpins that partition into the membrane as a single unit. Further, the insertion effic ...
Study of Different Variants of Mo Enzyme crARC and the Interaction
... stability of crARC activity at 22 and 4 ◦ C by determining its NHC reduction activity at different times. The NHC substrate selected to be reduced in this assay was the model substrate benzamidoxime [4]. The crARC reduction activity was determined in vitro in two ways, using either dithionite or NAD ...
... stability of crARC activity at 22 and 4 ◦ C by determining its NHC reduction activity at different times. The NHC substrate selected to be reduced in this assay was the model substrate benzamidoxime [4]. The crARC reduction activity was determined in vitro in two ways, using either dithionite or NAD ...
How flexible is α-actinin`s rod domain?
... Abstract: α−actinin, an actin binding protein, plays a Endo, and Ebashi (1967)]. On the other hand, in nonkey role in cell migration, cross-links actin filaments in muscle cells, α-actinin organizes the cortical cytoskelethe Z-disk, and is a major component of contractile mus- ton adjacent to membra ...
... Abstract: α−actinin, an actin binding protein, plays a Endo, and Ebashi (1967)]. On the other hand, in nonkey role in cell migration, cross-links actin filaments in muscle cells, α-actinin organizes the cortical cytoskelethe Z-disk, and is a major component of contractile mus- ton adjacent to membra ...
Applied Environmnetal Microbiology
... Expression of the cryV1 and cryV465 genes in E. coli. E. coli cells harboring the cryV1 or cryV465 gene failed to produce the toxin protein. It seems that transcription from the lac promoter of pUC19 is prevented by the transcriptional terminator structure of the cryI gene present upstream of the cr ...
... Expression of the cryV1 and cryV465 genes in E. coli. E. coli cells harboring the cryV1 or cryV465 gene failed to produce the toxin protein. It seems that transcription from the lac promoter of pUC19 is prevented by the transcriptional terminator structure of the cryI gene present upstream of the cr ...
Pavel Doležal
... possessing double-membrane bound organelles such as hydrogen-producing hydrogenosomes and newly characterized organelles, the mitosomes. These organelles were suggested to evolve either from a common pro-mitochondrial endosymbiont, or they represent highly modified or reduced mitochondria. Although ...
... possessing double-membrane bound organelles such as hydrogen-producing hydrogenosomes and newly characterized organelles, the mitosomes. These organelles were suggested to evolve either from a common pro-mitochondrial endosymbiont, or they represent highly modified or reduced mitochondria. Although ...
Evolutionary Relationships and Protein Domain Architecture in an
... significantly longer (100 aa), was subsequently found not only at the N-terminus of kinetoplastid calpain-like proteins, but also as the core element of a large number of short open reading frames, with an average length of approximately 200 amino acids. Six short sequences were found in T. brucei, ...
... significantly longer (100 aa), was subsequently found not only at the N-terminus of kinetoplastid calpain-like proteins, but also as the core element of a large number of short open reading frames, with an average length of approximately 200 amino acids. Six short sequences were found in T. brucei, ...
Phagosome maturation in unicellular eukaryote Paramecium: the
... the gene, protein and cellular level indicate that the set of components involved in this process and known up to now in mammalian cells is functioning in unicellular eukaryote. Rab7-interacting partners: homologues of its effector RILP (Rab-interacting lysosomal protein) and LAMP-2 (lysosomal membr ...
... the gene, protein and cellular level indicate that the set of components involved in this process and known up to now in mammalian cells is functioning in unicellular eukaryote. Rab7-interacting partners: homologues of its effector RILP (Rab-interacting lysosomal protein) and LAMP-2 (lysosomal membr ...
The Arabidopsis TRM1-TON1 interaction reveals a recruitment
... directed against two peptides of TRM1 defined in regions of minimal similarity with TRM2. On immunoblots, this antibody recognized a protein band of an apparent molecular mass of ;120 kD not present in the TRM1 loss-of-function mutant allele (Lee et al., 2006), which confirmed its specificity toward ...
... directed against two peptides of TRM1 defined in regions of minimal similarity with TRM2. On immunoblots, this antibody recognized a protein band of an apparent molecular mass of ;120 kD not present in the TRM1 loss-of-function mutant allele (Lee et al., 2006), which confirmed its specificity toward ...
Supplemental Methods 1. Amino acid conformation clustering Amino
... and the distribution information of the members in the clusters are listed in Table S6. 2. Protein atomistic non-covalent interacting database Atomistic contact interactions in proteins of known structures were organized into a database containing non-covalent atomistic interaction information for a ...
... and the distribution information of the members in the clusters are listed in Table S6. 2. Protein atomistic non-covalent interacting database Atomistic contact interactions in proteins of known structures were organized into a database containing non-covalent atomistic interaction information for a ...
The Arabidopsis TRM1-TON1 interaction reveals a recruitment
... directed against two peptides of TRM1 defined in regions of minimal similarity with TRM2. On immunoblots, this antibody recognized a protein band of an apparent molecular mass of ;120 kD not present in the TRM1 loss-of-function mutant allele (Lee et al., 2006), which confirmed its specificity toward ...
... directed against two peptides of TRM1 defined in regions of minimal similarity with TRM2. On immunoblots, this antibody recognized a protein band of an apparent molecular mass of ;120 kD not present in the TRM1 loss-of-function mutant allele (Lee et al., 2006), which confirmed its specificity toward ...
BIOCHEMISTRY I (CHMI 2227 E) PROBLEMS and
... 2227E), as offered at Laurentian University. It contains several problems taken from textbooks and from the author’s imagination. While the vast majority of the problems found in this book can be relatively easily solved with the help of the class notes, more difficult questions have also been inclu ...
... 2227E), as offered at Laurentian University. It contains several problems taken from textbooks and from the author’s imagination. While the vast majority of the problems found in this book can be relatively easily solved with the help of the class notes, more difficult questions have also been inclu ...
Coarse-grained normal mode analysis in structural biology
... by site-directed mutagenesis (e.g. correlated mutations), H/D exchange data and other biochemical (e.g. crosslinking) experiments. The free energy changes associated with H/D exchange of individual amino acids measured near native state conditions for a series of proteins have been correlated, for e ...
... by site-directed mutagenesis (e.g. correlated mutations), H/D exchange data and other biochemical (e.g. crosslinking) experiments. The free energy changes associated with H/D exchange of individual amino acids measured near native state conditions for a series of proteins have been correlated, for e ...
Dietary Guidelines should reflect new understandings about adult
... lean tissue mass and must increase as a percentage of low energy diets. The Food and Nutrition Board recognized the potential for biological diversity and individual choice with the DRI for macronutrients and created an Acceptable Macronutrient Distribution Range (AMDR)(14). The AMDR for protein pro ...
... lean tissue mass and must increase as a percentage of low energy diets. The Food and Nutrition Board recognized the potential for biological diversity and individual choice with the DRI for macronutrients and created an Acceptable Macronutrient Distribution Range (AMDR)(14). The AMDR for protein pro ...