Mycoplasma genitalium
... their reduced genome size and content rel73,351 base pairs (bp)] that contained a total of 3,806,280 bp of primary DNA ative to other prokaryotes. Mycoplasma genitalium is thought to con- sequence data. Contigs were ordered by ASM ALIGN, a program that links contain the smallest genome for a self-re ...
... their reduced genome size and content rel73,351 base pairs (bp)] that contained a total of 3,806,280 bp of primary DNA ative to other prokaryotes. Mycoplasma genitalium is thought to con- sequence data. Contigs were ordered by ASM ALIGN, a program that links contain the smallest genome for a self-re ...
Lecture 5 Tues 4-11-06
... 5. Toxo and plasmodium leader sequences function interchangeably. Chloroplast TPs from plants can also substitute for apicoplast TP. 6. TIC and TOC homologues are in apicoplasts. 7. Unclear if apicoplast is proximal or distal to Golgi. Legend: (a) Translation of protein with signal peptide followed ...
... 5. Toxo and plasmodium leader sequences function interchangeably. Chloroplast TPs from plants can also substitute for apicoplast TP. 6. TIC and TOC homologues are in apicoplasts. 7. Unclear if apicoplast is proximal or distal to Golgi. Legend: (a) Translation of protein with signal peptide followed ...
Slide 1
... • Some are glycerol based, but most are sphingosine based • Glycosphingolipids • Cerebrosides • Gangliosides ...
... • Some are glycerol based, but most are sphingosine based • Glycosphingolipids • Cerebrosides • Gangliosides ...
8/18/2015 1 BCMB 3100
... amino acid residues (Free-energy change for transfer of an amino acid from interior of a lipid bilayer to water) The larger the hydropathy, the greater the tendency of an amino acid to prefer a hydrophobic environment ...
... amino acid residues (Free-energy change for transfer of an amino acid from interior of a lipid bilayer to water) The larger the hydropathy, the greater the tendency of an amino acid to prefer a hydrophobic environment ...
Identification of Pexl3p, a Peroxisomal Membrane Receptor for the
... are imported posttranslationally (for review see Lazarow and Fujiki, 1985). Import of peroxisomal matrix proteins requires both ATP and cytosolic factors (Imanaka et al., 1987; Wendland and Subramani, 1993), and recent evidence suggests that peroxisomal proteins can be imported in a folded state (Mc ...
... are imported posttranslationally (for review see Lazarow and Fujiki, 1985). Import of peroxisomal matrix proteins requires both ATP and cytosolic factors (Imanaka et al., 1987; Wendland and Subramani, 1993), and recent evidence suggests that peroxisomal proteins can be imported in a folded state (Mc ...
Intracellular transport
... → minus motors experience larger force than plus motors, are more likely to unbind remaining minus motors experience even larger force Cascade of unbinding until only plus motors left ...
... → minus motors experience larger force than plus motors, are more likely to unbind remaining minus motors experience even larger force Cascade of unbinding until only plus motors left ...
Contribution of defined amino acid residues to the immunogenicity
... STh have been mapped to a highly conserved domain including six cysteine residues forming three intramolecular disul¢de bonds that are absolutely necessary for toxicity of the molecule [5]. Because STa is non-immunogenic in its native form, several di¡erent approaches have been explored to obtain no ...
... STh have been mapped to a highly conserved domain including six cysteine residues forming three intramolecular disul¢de bonds that are absolutely necessary for toxicity of the molecule [5]. Because STa is non-immunogenic in its native form, several di¡erent approaches have been explored to obtain no ...
Document
... The rather specific sugar elimination most easily can be explained by specific and active transport of sugar at one of the membrane sites in the secreCHNEPF in the preceding tory gland system as discussed in section 2 and by S review. Additional compounds associated with sugar in the nectar might be ...
... The rather specific sugar elimination most easily can be explained by specific and active transport of sugar at one of the membrane sites in the secreCHNEPF in the preceding tory gland system as discussed in section 2 and by S review. Additional compounds associated with sugar in the nectar might be ...
Document
... Examples: Keratin is the protein of hair, horns, feathers, and other skin appendages. Insects and spiders use silk fibers to make their cocoons and webs, respectively. Collagen and elastin proteins provide a fibrous framework in animal connective tissues. ...
... Examples: Keratin is the protein of hair, horns, feathers, and other skin appendages. Insects and spiders use silk fibers to make their cocoons and webs, respectively. Collagen and elastin proteins provide a fibrous framework in animal connective tissues. ...
Mitochondria, Chloroplasts, and Peroxisomes
... Mitochondria contain their own genetic system, which is separate and distinct from the nuclear genome of the cell. Mitochondria are thought to have evolved from bacteria that developed a symbiotic relationship in which they lived within larger cells (endosymbiosis). This hypothesis has recently been ...
... Mitochondria contain their own genetic system, which is separate and distinct from the nuclear genome of the cell. Mitochondria are thought to have evolved from bacteria that developed a symbiotic relationship in which they lived within larger cells (endosymbiosis). This hypothesis has recently been ...
Membrane protein integration into the endoplasmic reticulum
... of them containing charged residues [49–51]. These observations, together with the fact that TRAM itself contains an unusually high number of charged residues within its TM segments, led to the idea that TRAM could act as a chaperone for the integration of nonoptimal TM segments by providing a more ...
... of them containing charged residues [49–51]. These observations, together with the fact that TRAM itself contains an unusually high number of charged residues within its TM segments, led to the idea that TRAM could act as a chaperone for the integration of nonoptimal TM segments by providing a more ...
Print - Circulation Research
... in which sucrose was present only during the degradation period (i.e., its effects on proteolysis reflected changes during the first day of exposure). In addition, a second group of experiments was performed in which hearts were exposed to sucrose during the 24-hour stabilization, pulsation, and was ...
... in which sucrose was present only during the degradation period (i.e., its effects on proteolysis reflected changes during the first day of exposure). In addition, a second group of experiments was performed in which hearts were exposed to sucrose during the 24-hour stabilization, pulsation, and was ...
University of Groningen Cross-linking of dimeric CitS and GltS
... and includes the Ion Transporter (IT) superfamily ...
... and includes the Ion Transporter (IT) superfamily ...
Solubility-enhancing proteins MBP and NusA play a passive role in
... fusion proteins by TEV protease, GFP and DHFR, have biological activities that can be measured. We, therefore, sought to perform a more quantitative assessment of folding eYciency in these cases. To this end, the Xuorescence intensity of soluble extracts prepared from an equal number of cells produc ...
... fusion proteins by TEV protease, GFP and DHFR, have biological activities that can be measured. We, therefore, sought to perform a more quantitative assessment of folding eYciency in these cases. To this end, the Xuorescence intensity of soluble extracts prepared from an equal number of cells produc ...
Nucleosomes released from oviduct nuclei during brief micrococcal
... Two-dimensional analysis of the DNA of the electrophoretically separated 1SF nucleosomes. 1SF nucleosomes were separated by electrophoresis on DNP gels in the first dimension (left to right) and then run into a denaturing 12% polyacrylamide gel (top to b o t t o m ) . ...
... Two-dimensional analysis of the DNA of the electrophoretically separated 1SF nucleosomes. 1SF nucleosomes were separated by electrophoresis on DNP gels in the first dimension (left to right) and then run into a denaturing 12% polyacrylamide gel (top to b o t t o m ) . ...
α-Hemolysin pore formation into a supported
... bilayer of small unilamellar vesicles [5,6]. These works represent considerable technical advances for membrane protein studies as well as a promising alternative to cell-based membrane protein preparation. However, quantitative experimental approaches to characterize the concurrent expression and i ...
... bilayer of small unilamellar vesicles [5,6]. These works represent considerable technical advances for membrane protein studies as well as a promising alternative to cell-based membrane protein preparation. However, quantitative experimental approaches to characterize the concurrent expression and i ...
TIBS review article by Killian & Heijne
... (a) Amino acid sequences of WALP and KALP peptides. The N termini are acetylated and the C termini are blocked with either ethanolamine or amide. The flanking residues W and K are highlighted in red and green, respectively. (b) Model of the effect of decreasing the relative length of WALP and KALP p ...
... (a) Amino acid sequences of WALP and KALP peptides. The N termini are acetylated and the C termini are blocked with either ethanolamine or amide. The flanking residues W and K are highlighted in red and green, respectively. (b) Model of the effect of decreasing the relative length of WALP and KALP p ...
High-resolution mapping of protein sequence
... structures of ~50 amino acids9. These domains serve as proteinprotein interaction modules, binding to one of several conserved peptide motifs (Fig. 1a). We displayed a WW domain because its structure has been solved and because the folding, thermodynamic stability and binding preferences of WW domai ...
... structures of ~50 amino acids9. These domains serve as proteinprotein interaction modules, binding to one of several conserved peptide motifs (Fig. 1a). We displayed a WW domain because its structure has been solved and because the folding, thermodynamic stability and binding preferences of WW domai ...
HiFliQ Ni-NTA FPLC Columns - User Guide
... It is imperative that the lysate is completely clear prior to loading on the column as any particulate matter (e.g. cell debris) may partially foul and clog-up the resin resulting in an increased back pressure and reduced flow rates. This will significantly increase the binding, washing and elution ...
... It is imperative that the lysate is completely clear prior to loading on the column as any particulate matter (e.g. cell debris) may partially foul and clog-up the resin resulting in an increased back pressure and reduced flow rates. This will significantly increase the binding, washing and elution ...
Syllabus for GUTS lecture on Amino Acids
... Like Ka, pKa is a constant. Looking at this relationship you can see that when the ratio [A]/[HA] = 1.0, log of 1.0 = 0, and pH = pKa. Thus one way to think of pKa is that the value is equal to the pH at which 50% of an acid or base will be protonated and 50% will not. A pH of approximately 2.2 is ...
... Like Ka, pKa is a constant. Looking at this relationship you can see that when the ratio [A]/[HA] = 1.0, log of 1.0 = 0, and pH = pKa. Thus one way to think of pKa is that the value is equal to the pH at which 50% of an acid or base will be protonated and 50% will not. A pH of approximately 2.2 is ...
PHYLOGENETIC ANALYSIS OF PDV MOVEMENT PROTEIN
... The results obtained in the present study concerning a comparison of the MP and RBD MP amino acid sequences among Prune dwarf virus demonstrate the existence of some level of differentiation. The MP amino acid sequences of MP differed by 4.55% on average, i.e. not more than 27 aa. These results are ...
... The results obtained in the present study concerning a comparison of the MP and RBD MP amino acid sequences among Prune dwarf virus demonstrate the existence of some level of differentiation. The MP amino acid sequences of MP differed by 4.55% on average, i.e. not more than 27 aa. These results are ...