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PDF Format - Kinexus Bioinformatics Corporation
PDF Format - Kinexus Bioinformatics Corporation

... substrates in lysates from cultured cells. Clients should contact Kinexus if they are interested in having rabbit polyclonal antibodies developed to recognize novel proteins identified from our mass spectrometry analyses. ...
Nuclear accumulation of hepatitis B virus preS fragments
Nuclear accumulation of hepatitis B virus preS fragments

... Fig. 1. Functional domains within the large hepatitis B surface protein (LHBs) and the amino acid sequences of preS(1-174) region of HBV (adr subtype). (A) The LHBs consists of the preS and S domains, of which the S protein contains four membrane-spanning regions. Depending on the orientation of the ...
the Sec7 family of guanine-nucleotide- exchange factors
the Sec7 family of guanine-nucleotide- exchange factors

... Like other GTPases, ARFs cycle between active GTPbound and inactive GDP-bound conformations. For most ARFs, the GDP-bound form is primarily cytosolic, whereas ARF–GTP is membrane bound (ARF6 was originally thought to associate constitutively with membranes, but this has recently been called into que ...
Biochemistry of Amino acid
Biochemistry of Amino acid

... A tetrahedral carbon atom with 4 distinct constituents is said to be chiral. The one amino acid not exhibiting chirality is glycine since its '"Rgroup" is a hydrogen atom. Chirality describes the handedness of a molecule that is observable by the ability of a molecule to rotate the plane of polarize ...
Bilayers as Protein Solvents: Role of Bilayer Structure and Elastic
Bilayers as Protein Solvents: Role of Bilayer Structure and Elastic

... the interaction of protein amino acid residues with different membrane models. Several types of membrane model systems are used and discussed, including nonpolar solvents, such as 1-octanol or cyclohexane, as well as one-component lipid bilayers, such as dioleoylphosphatidylcholine (DOPC) or palmito ...
The Ubiquitin System for Protein Degradation and Some of Its Roles
The Ubiquitin System for Protein Degradation and Some of Its Roles

Lab Session 9
Lab Session 9

... • In the absence of a stacking gel, your sample would sit on top of the running gel, as a band of up to 1cm deep. • Rather than being lined up together and hitting the running gel together, the proteins in the sample would all enter the running gel at different times, resulting in very smeared bands ...
Osmotic Signaling in Plants. Multiple Pathways
Osmotic Signaling in Plants. Multiple Pathways

... released from intracellular stores in response to hyperosmotic stresses as a result of the activation of the IP3dependent calcium channels. Other phospholipids, in particular phosphatidic acid (PA), seem to have important roles in osmotic signaling (Munnik and Meijer, 2001). PA was reported to accum ...
Intrinsic Disorder in Cell-signaling and Cancer
Intrinsic Disorder in Cell-signaling and Cancer

GST SF in E. coli - Institute for Genomic Biology
GST SF in E. coli - Institute for Genomic Biology

... YidA(HAD): long lag when cells are resuspended in galactonate ...
9c63$$mr30 Black separation
9c63$$mr30 Black separation

... disease. In vitro, Treponema denticola produces a large number of purported virulence factors, including tissue-degrading enzymes, cytotoxic factors [13 – 21], and at least two types of proteins, which interact with selected host cells [22 – 24]. These factors, if functional in the in vivo environme ...
Effects of Anions on the Positive Ion Electrospray Ionization Mass
Effects of Anions on the Positive Ion Electrospray Ionization Mass

... > trifluoroacetate > bromide > chloride. We and others have shown previously“q8-l2 that conformational changes in proteins, whether produced by changes in pH, temperature, or the presenceof chemical denaturants, can produce profound changes in the distribution of charge states observed from electros ...
Tubular reabsorption
Tubular reabsorption

... Both bind to one carrier protein to be transported together. Thus, secondary active transport does not require energy directly from ATP. ...
Knox, Kirstin : An Introduction To Motif Based Functional Classification of Large Protein Families
Knox, Kirstin : An Introduction To Motif Based Functional Classification of Large Protein Families

... Malnic et al 1999). The mammalian olfactory system has the ability to detect and discriminate between an astounding array of chemical compounds that may be present in the external ...
Free amino acids are important for the retention of protein and non
Free amino acids are important for the retention of protein and non

... concentration of amino acids in the midgut lumen after a blood meal is conceivable as a result of the presence of free amino acids in the blood (60–140 µg/ml)( Everett, 1942; Forslund et al., 2000), combined with the enzymatic activity of exopeptidases and other proteases on the blood polypeptides ( ...
Identification of a novel viral protein in infectious bursal disease
Identification of a novel viral protein in infectious bursal disease

... various IPNV strains, i.e. strain Jasper (Duncon & Dobos, 1986) and N1 (Havarstein et al., 1990), possess a second ORF partially overlapping the polyprotein gene (Havarstein et al., 1990) and a 17 kDa protein has been demonstrated by SDS PAGE of [a~S]methionine-labelled purified IPNV. However, no co ...
mitochondria
mitochondria

... in detail in the next section), which takes place in the inner mitochondrial membrane. The high-energy electrons from NADH and FADH2 are transferred through a series of carriers in the membrane to molecular oxygen. The energy derived from these electron transfer reactions is converted to potential e ...
Macromolecular Transport between the Nucleus and the Cytoplasm
Macromolecular Transport between the Nucleus and the Cytoplasm

... signal that has emerged from such an approach is termed a Proline Tyrosine (PY)-NLS, which is found in a number of RNA binding proteins [33]. Interestingly, the PY-NLS is defined not so much by the primary amino acid sequence but more by general structural features, making this motif challenging to i ...
Protein Arginine Methylation in Candida albicans: Role
Protein Arginine Methylation in Candida albicans: Role

... of ␦-MMA, in which the internal ␦ nitrogen of arginine is methylated (29). Although the Rmt2 sequence is slightly similar to that of a mammalian small-molecule methyltransferase that targets guanidinoacetate (29), more closely related genes are found in fungal and plant genomes but are absent in ani ...
The goal of protein structure prediction by threading is to find a best
The goal of protein structure prediction by threading is to find a best

... commonly used by macromolecular modeling software. Instead, most threading objective functions are determined empirically by statistical analysis of the 3D data deposited in the Protein Data Bank (PDB). Thus they are often referred to as empirical potentials or knowledge-based potentials. In the cas ...
Topic guide 1.1: Amino acids and proteins
Topic guide 1.1: Amino acids and proteins

... and are important building blocks that enable growth and repair. Proteins are made from carbon, oxygen, hydrogen and nitrogen. They have many functions within the body, for instance, structural support, antibodies, membrane carriers, enzymes or hormones, and each protein has its own specific functio ...
Reconstitution of Outer Membrane Protein Assembly from Purified
Reconstitution of Outer Membrane Protein Assembly from Purified

... b-barrel membrane proteins in Gram-negative bacteria, mitochondria, and chloroplasts are assembled by highly conserved multi-protein complexes. The mechanism by which these molecular machines fold and insert their substrates is poorly understood. It has not been possible to dissect the folding and i ...
Update on chloroplast research: new tools, new
Update on chloroplast research: new tools, new

... Transcriptomics: Reaching the Next Level: Guilt-byAssociation Approaches It was initially expected that transcriptome analyses would directly elucidate the function of genes based on a simple comparison of the transcript profile of wild-type with that of any given mutant. It soon became clear, howev ...
Localization of Protein-Protein lnteractions between Subunits of
Localization of Protein-Protein lnteractions between Subunits of

Biochemistry of Sulfur
Biochemistry of Sulfur

... The predicted orientation and the molecular composition of the Ac. ambivalens SR deduced from the biochemical results and the sequence analysis are similar to the W. succinogenes PSR (Fig. 7). Both enzymes consist of homologous catalytic and electron transfer subunits (SreA/PsrA and SreB/PsrB, resp ...
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Magnesium transporter

This page links directly from the magnesium in biological systems page.Magnesium transporters are proteins that transport magnesium across the cell membrane. All forms of life require magnesium, yet the molecular mechanisms of Mg2+ uptake from the environment and the distribution of this vital element within the organism are only slowly being elucidated.In bacteria, Mg2+ is probably mainly supplied by the CorA protein and, where the CorA protein is absent, by the MgtE protein. In yeast the initial uptake is via the Alr1p and Alr2p proteins, but at this stage the only internal Mg2+ distributing protein identified is Mrs2p. Within the protozoa only one Mg2+ transporter (XntAp) has been identified. In metazoa, Mrs2p and MgtE homologues have been identified, along with two novel Mg2+ transport systems TRPM6/TRPM7 and PCLN-1. Finally, in plants, a family of Mrs2p homologues has been identified along with another novel protein, AtMHX.
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