Update on chloroplast research: new tools, new
... Transcriptomics: Reaching the Next Level: Guilt-byAssociation Approaches It was initially expected that transcriptome analyses would directly elucidate the function of genes based on a simple comparison of the transcript profile of wild-type with that of any given mutant. It soon became clear, howev ...
... Transcriptomics: Reaching the Next Level: Guilt-byAssociation Approaches It was initially expected that transcriptome analyses would directly elucidate the function of genes based on a simple comparison of the transcript profile of wild-type with that of any given mutant. It soon became clear, howev ...
Biochemistry of Sulfur
... The predicted orientation and the molecular composition of the Ac. ambivalens SR deduced from the biochemical results and the sequence analysis are similar to the W. succinogenes PSR (Fig. 7). Both enzymes consist of homologous catalytic and electron transfer subunits (SreA/PsrA and SreB/PsrB, resp ...
... The predicted orientation and the molecular composition of the Ac. ambivalens SR deduced from the biochemical results and the sequence analysis are similar to the W. succinogenes PSR (Fig. 7). Both enzymes consist of homologous catalytic and electron transfer subunits (SreA/PsrA and SreB/PsrB, resp ...
Production of Polyclonal Antibodies against Sucrose Transporter
... INTRODUCTION Sucrose is one of the most common and abundant carbon forms in plants. Most plants synthesize sucrose as a major photosynthetic product and use it for long distance carbon transport. Therefore sucrose transport in plants probably is highly regulated and sucrose transporters have indispe ...
... INTRODUCTION Sucrose is one of the most common and abundant carbon forms in plants. Most plants synthesize sucrose as a major photosynthetic product and use it for long distance carbon transport. Therefore sucrose transport in plants probably is highly regulated and sucrose transporters have indispe ...
Localization of Protein-Protein lnteractions between Subunits of
... al., 1989) and inserted into pME10. Initially, an Xmnl fragment encoding amino acids V623-E1048, nearly all of the carboxyterminal half, was ligated into the Smal site of pME10. As expected, this construct, pME10/PC623-1048, repressed (3-galactosidase activity as IPTG levels increased. pME10/ PC623- ...
... al., 1989) and inserted into pME10. Initially, an Xmnl fragment encoding amino acids V623-E1048, nearly all of the carboxyterminal half, was ligated into the Smal site of pME10. As expected, this construct, pME10/PC623-1048, repressed (3-galactosidase activity as IPTG levels increased. pME10/ PC623- ...
Text S1.
... “We have used both the water-vapor transfer free energies and the interior-exterior distribution of amino acid side-chains determined by Chothia (1976) in assigning the final hydropathy values (Table 2). Results presented later in this paper indicate clearly that the number in the second place of th ...
... “We have used both the water-vapor transfer free energies and the interior-exterior distribution of amino acid side-chains determined by Chothia (1976) in assigning the final hydropathy values (Table 2). Results presented later in this paper indicate clearly that the number in the second place of th ...
Ro52: Structure and interactions of constructs of RING and B-box
... (NMR) spectroscopy and auto-ubiquitination assay by Western blot. The goal was to gain deeper insight in structural and functional properties of these domains. In the end only two constructs were investigated because of time limitations. It was shown by NMR that one construct has similar structure a ...
... (NMR) spectroscopy and auto-ubiquitination assay by Western blot. The goal was to gain deeper insight in structural and functional properties of these domains. In the end only two constructs were investigated because of time limitations. It was shown by NMR that one construct has similar structure a ...
Metabolism of “surplus” amino acids
... For an adult in N balance, apart from small amounts of amino acids required for the synthesis of neurotransmitters, hormones, etc, an amount of amino acids almost equal to that absorbed from the diet can be considered to be “surplus” in that it will be catabolized. The higher diet-induced thermogene ...
... For an adult in N balance, apart from small amounts of amino acids required for the synthesis of neurotransmitters, hormones, etc, an amount of amino acids almost equal to that absorbed from the diet can be considered to be “surplus” in that it will be catabolized. The higher diet-induced thermogene ...
1. Guri Giaever, Patrick Flaherty, Jochen Kumm, Michael Proctor, Corey Nislow, Daniel F. Jaramillo, Angela M. Chu, Michael I. Jordan, Adam P. Arkin, and Ronald W. Davis Chemogenomic profiling: Identifying the functional interactions of small molecules in yeast PNAS 2004 101: 793-798.
... purines (8). Its antineoplastic activity is thought to result primarily from intrastrand DNA crosslinking. Because cisplatin has no protein target, we expected that the HIP assay would reveal few, if any, sensitive heterozygous strains. In fact, in seven experiments covering a 4-fold concentration r ...
... purines (8). Its antineoplastic activity is thought to result primarily from intrastrand DNA crosslinking. Because cisplatin has no protein target, we expected that the HIP assay would reveal few, if any, sensitive heterozygous strains. In fact, in seven experiments covering a 4-fold concentration r ...
Cell - Notes Milenge
... localized to the outer mitochondrial membrane seemed to fly in the face of this organelle’s purported responsiveness to changes in intracellular Ca2+ flux.[24] But the presence of the MAM resolves this apparent contradiction: the close physical association between the two organelles results in Ca2+ ...
... localized to the outer mitochondrial membrane seemed to fly in the face of this organelle’s purported responsiveness to changes in intracellular Ca2+ flux.[24] But the presence of the MAM resolves this apparent contradiction: the close physical association between the two organelles results in Ca2+ ...
March - Gentle Rain
... into a probiotic food. Cultured veggies are easier to digest than raw or even cooked vegetables. Cultured Vegetables are made by shredding cabbage or a combination of cabbage and other vegetables, packing them tightly into an air-tight container, and leaving them at room temperature to ferment for 3 ...
... into a probiotic food. Cultured veggies are easier to digest than raw or even cooked vegetables. Cultured Vegetables are made by shredding cabbage or a combination of cabbage and other vegetables, packing them tightly into an air-tight container, and leaving them at room temperature to ferment for 3 ...
Pairwise alignment
... to the probability that amino acid i mutates into amino acid j for all pairs of amino acids. Substitution matrices are constructed by assembling a large and diverse sample of verified pairwise alignments (or multiple sequence alignments) of amino acids. Substitution matrices should reflect the true ...
... to the probability that amino acid i mutates into amino acid j for all pairs of amino acids. Substitution matrices are constructed by assembling a large and diverse sample of verified pairwise alignments (or multiple sequence alignments) of amino acids. Substitution matrices should reflect the true ...
AlgPred: prediction of allergenic proteins and mapping of
... of allergies are rhinitis, asthma and atopic eczema, but more severe reactions such as acute and fatal anaphylactic shock can also occur. It affects a large population with very high prevalence particularly of skin sensitization (4,5). Most allergic responses occur on mucous membrane surface in resp ...
... of allergies are rhinitis, asthma and atopic eczema, but more severe reactions such as acute and fatal anaphylactic shock can also occur. It affects a large population with very high prevalence particularly of skin sensitization (4,5). Most allergic responses occur on mucous membrane surface in resp ...
Biogenesis of the Protein Storage Vacuole Crystalloid
... proteins. When expressed in transgenic tobacco seeds, the bean seed storage proteins, phaseolin and phytohemagglutinin, were localized to the PSV matrix (Greenwood and Chrispeels, 1985; Hoffman et al., 1987; Sturm and Chrispeels, 1988), whereas the maize 15-kD zein was localized to the PSV crystallo ...
... proteins. When expressed in transgenic tobacco seeds, the bean seed storage proteins, phaseolin and phytohemagglutinin, were localized to the PSV matrix (Greenwood and Chrispeels, 1985; Hoffman et al., 1987; Sturm and Chrispeels, 1988), whereas the maize 15-kD zein was localized to the PSV crystallo ...
L22_GlngBox
... Transport of FA: Mitochondria • Malonyl CoA is a very strong inhibitor of CAT-I • CAT-I is the key regulator of fat oxidation - once FA gets into the mitochondria, it will be oxidized (i.e. the only fate of mitochondrial FA-CoA is oxidation) • Alternative fate of FA-CoA in the cytoplasm is esterifi ...
... Transport of FA: Mitochondria • Malonyl CoA is a very strong inhibitor of CAT-I • CAT-I is the key regulator of fat oxidation - once FA gets into the mitochondria, it will be oxidized (i.e. the only fate of mitochondrial FA-CoA is oxidation) • Alternative fate of FA-CoA in the cytoplasm is esterifi ...
Which of the following statements best describes why Experiment 1
... The distribution curves show that, over the course of time, the strains of bacteria which can be treated using any specific antibiotic die off, leaving a population of bacteria consisting solely of drug-resistant strains. If we start with a strain of bacteria which we know to be resistant to the m ...
... The distribution curves show that, over the course of time, the strains of bacteria which can be treated using any specific antibiotic die off, leaving a population of bacteria consisting solely of drug-resistant strains. If we start with a strain of bacteria which we know to be resistant to the m ...
Comparison of Rumen Amino Acid Protection Technologies
... Increased milk yield…particularly in early lactation cows Reduced requirement for RUP for similar or higher milk milk component levels and milk yields More predictable changes in milk production to changes in RUP supply ...
... Increased milk yield…particularly in early lactation cows Reduced requirement for RUP for similar or higher milk milk component levels and milk yields More predictable changes in milk production to changes in RUP supply ...
Modification of the signal sequence cleavage site of
... across the membrane, in both Gram-negative and Grampositive bacteria (von Heijne, 1990; Pugsley, 1993; van Wely et al., 2001; Antelmann et al., 2001; and references therein). Upon translocation, the signal peptide is removed by the action of signal peptidases (Bron et al., 1998; Paetzel et al., 2000 ...
... across the membrane, in both Gram-negative and Grampositive bacteria (von Heijne, 1990; Pugsley, 1993; van Wely et al., 2001; Antelmann et al., 2001; and references therein). Upon translocation, the signal peptide is removed by the action of signal peptidases (Bron et al., 1998; Paetzel et al., 2000 ...
Biogenesis of the Protein Storage Vacuole Crystalloid
... proteins. When expressed in transgenic tobacco seeds, the bean seed storage proteins, phaseolin and phytohemagglutinin, were localized to the PSV matrix (Greenwood and Chrispeels, 1985; Hoffman et al., 1987; Sturm and Chrispeels, 1988), whereas the maize 15-kD zein was localized to the PSV crystallo ...
... proteins. When expressed in transgenic tobacco seeds, the bean seed storage proteins, phaseolin and phytohemagglutinin, were localized to the PSV matrix (Greenwood and Chrispeels, 1985; Hoffman et al., 1987; Sturm and Chrispeels, 1988), whereas the maize 15-kD zein was localized to the PSV crystallo ...
Digital Coding of Amino Acids Based on Hydrophobic Index
... Viewing protein synthesis as an information processing system allows amino acid sequences to be analyzed as messages without considering the physical-chemical elements for information processing [4]. There are many established information process methods that can be used for the analysis of amino ac ...
... Viewing protein synthesis as an information processing system allows amino acid sequences to be analyzed as messages without considering the physical-chemical elements for information processing [4]. There are many established information process methods that can be used for the analysis of amino ac ...
The role of feed enzymes in poultry gut health
... additional benefits over E. coli equivalents, including much higher activity earlier in the digestive tract, minimising the antinutrient effects of phytate and maximising the time available for nutrient digestion and absorption. Contrary to the common paradigm, the risk for the producer is higher wi ...
... additional benefits over E. coli equivalents, including much higher activity earlier in the digestive tract, minimising the antinutrient effects of phytate and maximising the time available for nutrient digestion and absorption. Contrary to the common paradigm, the risk for the producer is higher wi ...