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Studying Protein Flexibility in a Statistical Framework: Tools and
Studying Protein Flexibility in a Statistical Framework: Tools and

... One basic question relates to the number of proteins motions and to what degree they divide up amongst the basic classification categories in the database. This can be answered on a number of levels, depending on our degree of knowledge about the motion. There are currently (21 September 2001) the f ...
Ammonium utilization in Bacillus subtilis: transport and regulatory
Ammonium utilization in Bacillus subtilis: transport and regulatory

... glutamicum and Streptomyces coelicolor, glutamine synthetase activity is also controlled by adenylation/deadenylation (Jakoby et al., 1999; Fink et al., 1999). Transcription of nitrogen-responsive genes is controlled by the AmtR and GlnR regulators, respectively, in these bacteria (Jakoby et al., 20 ...
Advances in affinity purification mass spectrometry of
Advances in affinity purification mass spectrometry of

Chapter 28 Discovery and Classification of Glycan
Chapter 28 Discovery and Classification of Glycan

... Membrane anchors in lectins can take multiple forms, but they often span the membrane, linking extracellular CRDs with cytoplasmic domains. This arrangement facilitates the flow of information between glycan-binding sites on the extracellular surface and the cytoplasm. Simple sequence motifs in t ...
BCA Assay
BCA Assay

The Escherichia coli mar Locus— Antibiotic Resistance and More
The Escherichia coli mar Locus— Antibiotic Resistance and More

... Diseases in Bethesda, Md. Moreover, MarA can also act directly as a repressor, and this function is apparently affected by the position of the MarA binding site (the marbox), according to our collaborator Thamarai Schneiders. Similarly Demple and his group report that Rob has repressor activity. Mar ...
Analysis of the glycoside hydrolase family 8 catalytic core in
Analysis of the glycoside hydrolase family 8 catalytic core in

... gaining importance as they can produce low molecular weight chitooligomers which are commercially important for the pharmaceutical, agricultural and food industries. Bacillus species which are common soil inhabitants are known to produce cellulasechitosanases [3]. Several cellulase-chitosanases have ...
PHL 424 2nd S
PHL 424 2nd S

...  Minocycline is usually the most active followed by doxycycline  They are also active against some protozoa, e.g., amebas  The antibacterial activities of most tetracyclines are similar except that tetracyclineresistant strains may remain susceptible to doxycycline or minocycline, drugs that are ...
Molecular Evolution and Structure of a
Molecular Evolution and Structure of a

... with the sequence in the TIGR database. As our PCR results imply, Ciona appears to have only a single gene for a-actinin. Previous comparative studies of gene families in Ciona have show that most genes that are present in multiple copies in vertebrates only have a single representative in Ciona (De ...
A Series of Ubiquitin Binding Factors Connects CDC48/p97 to
A Series of Ubiquitin Binding Factors Connects CDC48/p97 to

... (ubiquitin ligase) finally binds both the E2 and a substrate and catalyzes the transfer of ubiquitin to the substrate. Ubiquitin itself is often a substrate for further ubiquitylation, and proteins modified by such multiubiquitin chains are preferentially targeted for degradation by the proteasome. ...
Regulation of Ribosome Biogenesis by the Rapamycin
Regulation of Ribosome Biogenesis by the Rapamycin

... (Figure 2A). For comparison, we also examined mRNA levels for the G1 cyclin CLN1 as well as the heat-shock protein HSP26, both of which are representative of several mRNAs whose abundance either decreases or increases, respectively, upon rapamycin treatment (Barbet ...
Protein Kinases - School of Medicine
Protein Kinases - School of Medicine

... three amino acids with an –OH in their R-group – Serine – Threonine – Tyrosine ...
Origin of diderm (Gram-negative) bacteria
Origin of diderm (Gram-negative) bacteria

... or two membranes (diderms). It is important to understand how these and other variations that are observed in the cell envelopes of prokaryotic organisms have originated. In 2009, James Lake proposed that cells with two membranes (primarily Gramnegative bacteria) originated from an ancient endosymbi ...
Additional Methods_Office2004
Additional Methods_Office2004

... reference, by the methods and instruments described in 7. The extinction coefficient of the red form 570 was found by measuring the initial change in optical density (OD) of the red and green forms of protein following irradiation with 385 nm light, assuming in an incremental time period the loss i ...
Amino Acids 2 Questions
Amino Acids 2 Questions

... 2.  Gel‐filtration chromatography separates a mixture of proteins on the basis of:  A) size  B) charge C) affinity for ligands in the column matrix  D) density  3.  What is the purpose of treating a protein with 2‐mercaptoethanol?  A) To hydrolyze the protein into its amino acids. B) To derivatize a ...
Bacterial tail anchors can target to the mitochondrial outer
Bacterial tail anchors can target to the mitochondrial outer

... mitochondrial OM that would allow full integration of mitochondria within the ancestral ...
Evaluation of the Progress of Protein Hydrolysis
Evaluation of the Progress of Protein Hydrolysis

... hydrolysis has several aims. The most common is to make the protein moiety of a foodstuff soluble by reducing the size of the peptides. Solubilization simplifies isolation of the protein moiety by physical means. Protein hydrolysis has also been applied to improve the functional, organoleptic, and n ...
Pipecleaner Proteins Lab
Pipecleaner Proteins Lab

... Because proteins are smaller than microscopic, we would have a pretty hard time doing a hands-on lab on this topic. However, we can explore proteins in an indirect way through modeling. Everything in science is done with models – the scientific method itself is about modeling complex ideas into simp ...
Pipecleaner Proteins Lab
Pipecleaner Proteins Lab

... Because proteins are smaller than microscopic, we would have a pretty hard time doing a hands-on lab on this topic. However, we can explore proteins in an indirect way through modeling. Everything in science is done with models – the scientific method itself is about modeling complex ideas into simp ...
Co-opting sulphur-carrier proteins from primary metabolic pathways
Co-opting sulphur-carrier proteins from primary metabolic pathways

... For bond formation between carbon and sulphur in primary metabolites, the major ionic sulphur sources are the persulphide and thiocarboxylate groups on sulphur-carrier (donor) proteins3,4. Each group is post-translationally generated through the action of a specific activating enzyme. In all reporte ...
Amino acid
Amino acid

... PROTEIN SIZE (continued) • Proteins: • are extremely large natural polymers. • have molecular weights of ~6000 – several million u. • are too large to pass through cell membranes. • are contained inside the normal cells where they were formed. • can leak out if cell is damaged by disease or trauma. ...
Recombinant N-terminal Nucleotide
Recombinant N-terminal Nucleotide

as a PDF
as a PDF

... AKAP149 (Trendelenburg et al., 1996). Mouse homologues of D-AKAP1, as summarized in Fig. 1 A, share a 525–amino acid core, and differ at their COOH termini, presumably by alternative splicing. This core contains the R-binding domain that is responsible for interacting with the regulatory subunit. Th ...
Supplemental Methods and Figure Legends
Supplemental Methods and Figure Legends

... XhoI site was incorporated into the downstream primer for HHT2. The resulting plasmids (pRB633, HHT2; pRB591, HHF2) were used as templates for site-directed mutagenesis using the QuikChange Multi system (Stratagene). P. angusta H3 and H4 each differ from the respective S. cerevisiae proteins at thre ...
1. Introduction Chemotaxis Chemotaxis is the net movement of a
1. Introduction Chemotaxis Chemotaxis is the net movement of a

... phosphorylated CheB removes methyl groups from specific glutamyl residues of MCPs which are added by the constitutively active methyltransferase CheR [12, 25, 26]. This allows for greater sensitivity to a large ligand concentration range. Escherichia coli encodes five individual chemoreceptors; the ...
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Magnesium transporter

This page links directly from the magnesium in biological systems page.Magnesium transporters are proteins that transport magnesium across the cell membrane. All forms of life require magnesium, yet the molecular mechanisms of Mg2+ uptake from the environment and the distribution of this vital element within the organism are only slowly being elucidated.In bacteria, Mg2+ is probably mainly supplied by the CorA protein and, where the CorA protein is absent, by the MgtE protein. In yeast the initial uptake is via the Alr1p and Alr2p proteins, but at this stage the only internal Mg2+ distributing protein identified is Mrs2p. Within the protozoa only one Mg2+ transporter (XntAp) has been identified. In metazoa, Mrs2p and MgtE homologues have been identified, along with two novel Mg2+ transport systems TRPM6/TRPM7 and PCLN-1. Finally, in plants, a family of Mrs2p homologues has been identified along with another novel protein, AtMHX.
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