Yeasts as a model for human diseases
... who were in the field at the time, this came as a major breakthrough. Up to this time – even though J. Monod had already stated that ‘what is true for E. coli is true for the elephant’ – demonstrating that molecular details of functional pathways were conserved from one organism to another was neith ...
... who were in the field at the time, this came as a major breakthrough. Up to this time – even though J. Monod had already stated that ‘what is true for E. coli is true for the elephant’ – demonstrating that molecular details of functional pathways were conserved from one organism to another was neith ...
Defining the essential functional regions of the nucleoporin Nup145p
... remove a 2.7 kbp fragment and the resulting vector religated. The first 100 kDa (nup145-100) was placed in HIS3-CEN and URA3-2µ vectors by inserting the 4.8 kbp EcoRI fragment from pSW69 into pRS313 and pRS426 (Sikorski and Hieter, 1989) to form pSW360 and pSW249, respectively. pSW363, (nup145∆GLFG/ ...
... remove a 2.7 kbp fragment and the resulting vector religated. The first 100 kDa (nup145-100) was placed in HIS3-CEN and URA3-2µ vectors by inserting the 4.8 kbp EcoRI fragment from pSW69 into pRS313 and pRS426 (Sikorski and Hieter, 1989) to form pSW360 and pSW249, respectively. pSW363, (nup145∆GLFG/ ...
Antigenic Properties of Mycoplasma Organisms and Membranes
... Agglutination of whole cell. This was carried out either on glass slides at room temperature or in test-tubes at 52O according to Bailey et al. (1961). Double difliion in agar. The test was done according to Ouchterlony (1964). Noble agar 1% (w/v) was prepared in veronal+HCl buffer (PH = 8.2; ionic ...
... Agglutination of whole cell. This was carried out either on glass slides at room temperature or in test-tubes at 52O according to Bailey et al. (1961). Double difliion in agar. The test was done according to Ouchterlony (1964). Noble agar 1% (w/v) was prepared in veronal+HCl buffer (PH = 8.2; ionic ...
Phosphorylation of Bni4 by MAP kinases contributes to septum
... (μM) and are the average values from more than 50 cells (n > 50). They were performed on digital images using the tools included in the ImageJ software (NIH). ∗ indicates values significantly different from the wild type (P < 0.05). ...
... (μM) and are the average values from more than 50 cells (n > 50). They were performed on digital images using the tools included in the ImageJ software (NIH). ∗ indicates values significantly different from the wild type (P < 0.05). ...
Protein damage on Cu2+
... slightly during Cu2+-IDA chromatography. This was a 5-column volume run so there is the possibility of more inactivation with longer contact time runs (i.e. at a larger scale). – The requirements for metal, and the enhancement of activity loss by the presence of the reducing agent (ascorbate) and pe ...
... slightly during Cu2+-IDA chromatography. This was a 5-column volume run so there is the possibility of more inactivation with longer contact time runs (i.e. at a larger scale). – The requirements for metal, and the enhancement of activity loss by the presence of the reducing agent (ascorbate) and pe ...
Bacteria Binding by DMBT1/SAG/gp-340 Is Confined to
... creted proteins that are characterized by the presence of one or more SRCR domains. SRCR domains consist of ⬃110 amino acids and are divided into groups A and B based on the number of conserved cysteine residues, namely six for group A and eight for group B. The best studied members of the group A S ...
... creted proteins that are characterized by the presence of one or more SRCR domains. SRCR domains consist of ⬃110 amino acids and are divided into groups A and B based on the number of conserved cysteine residues, namely six for group A and eight for group B. The best studied members of the group A S ...
Sequential steps in clathrin-mediated synaptic vesicle endocytosis
... clathrin assembly and which appears to be critical for the generation of synaptic vesicles with an homogenous size. In Drosophila and C. elegans lacking AP180-like proteins, nerve terminals still contain synaptic vesicles, but their average size is larger and the size variability is increased when c ...
... clathrin assembly and which appears to be critical for the generation of synaptic vesicles with an homogenous size. In Drosophila and C. elegans lacking AP180-like proteins, nerve terminals still contain synaptic vesicles, but their average size is larger and the size variability is increased when c ...
Intracellular Protein Degradation
... While the experimental study involved the bacterial -galactosidase, the conclusions were broader, including also the authors’ hypothesis on mammalian proteins. The use of the term ‘dynamic state’ was not incidental, as they challenged directly Schoenheimer’s studies. Now, after more then six decade ...
... While the experimental study involved the bacterial -galactosidase, the conclusions were broader, including also the authors’ hypothesis on mammalian proteins. The use of the term ‘dynamic state’ was not incidental, as they challenged directly Schoenheimer’s studies. Now, after more then six decade ...
Correlation between the respiration-driven Na+ pump
... (mg protein)-'. Assuming that the internal cell volume is similar to that of V. costicola [3 pl (mg protein)-'] (Udagawa et al., 1986), the intracellular concentration of AIB was calculated to be 33 mM, which was 330-fold higher than that of the external medium. When 100 mM unlabelled AIB was added ...
... (mg protein)-'. Assuming that the internal cell volume is similar to that of V. costicola [3 pl (mg protein)-'] (Udagawa et al., 1986), the intracellular concentration of AIB was calculated to be 33 mM, which was 330-fold higher than that of the external medium. When 100 mM unlabelled AIB was added ...
Tissue-Specific Protein Expression in Plant Mitochondria
... 1989). Although these studies reveal that isolated mitochondria from different tissues or genotypes vary in the complement of proteins they synthesize, no conclusions can be drawn concerning the possible physiological or biochemical roles these unidentified proteins may play. The alternative oxidase ...
... 1989). Although these studies reveal that isolated mitochondria from different tissues or genotypes vary in the complement of proteins they synthesize, no conclusions can be drawn concerning the possible physiological or biochemical roles these unidentified proteins may play. The alternative oxidase ...
PDF - Blood Journal
... Phase separation of vesicle proteins in Triton X-114 solution The phase separation of membrane proteins in Triton X-114 according to the difference in hydrophobicity32 was applied with slight modifications. To purify Triton X-114, the precondensation of the detergent was performed 3 times, as recomm ...
... Phase separation of vesicle proteins in Triton X-114 solution The phase separation of membrane proteins in Triton X-114 according to the difference in hydrophobicity32 was applied with slight modifications. To purify Triton X-114, the precondensation of the detergent was performed 3 times, as recomm ...
BMC Cell Biology
... respectively [4-8], through the nuclear pore complexes [912]. The NLSs and NESs are recognized by the nucleocytoplasmic transport factors. Most nucleocytoplasmic transport factors belong to the family of karyopherin β protein known as importin-β [13]. Importin-β is a complex protein carrier and acts ...
... respectively [4-8], through the nuclear pore complexes [912]. The NLSs and NESs are recognized by the nucleocytoplasmic transport factors. Most nucleocytoplasmic transport factors belong to the family of karyopherin β protein known as importin-β [13]. Importin-β is a complex protein carrier and acts ...
Life in acid: pH homeostasis in acidophiles
... cellular protonation and, if left unchecked, will rapidly dissipate the DpH. The functions of proteins and nucleic acids are impaired by protonation, and interference caused by free intracellular protons can impair processes such as DNA transcription, protein synthesis and enzyme activities [10]. pH ...
... cellular protonation and, if left unchecked, will rapidly dissipate the DpH. The functions of proteins and nucleic acids are impaired by protonation, and interference caused by free intracellular protons can impair processes such as DNA transcription, protein synthesis and enzyme activities [10]. pH ...
Database searching
... Add Insertion and Deletion state. Estimate the emission probabilities according to amino acid counts in column. Different positions in the protein will have different emission ...
... Add Insertion and Deletion state. Estimate the emission probabilities according to amino acid counts in column. Different positions in the protein will have different emission ...
Structural Prediction of Membrane
... cal characteristics (e.g. hydrophobicity) for each amino acid known soluble protein structures which again are unlikely within the sequence. A plot of the additive function versus to reproduce the membrane environment given their interior sequence number results in maxima and minima indicating hydro ...
... cal characteristics (e.g. hydrophobicity) for each amino acid known soluble protein structures which again are unlikely within the sequence. A plot of the additive function versus to reproduce the membrane environment given their interior sequence number results in maxima and minima indicating hydro ...
Chapter 30: Protein Synthesis
... follows less stringent rules • That is, there is wobble in base pairing at this position. The third base is called “wobble ...
... follows less stringent rules • That is, there is wobble in base pairing at this position. The third base is called “wobble ...
Cell membranes
... surrounding by membrane Biomembrane are bilayer structure. the area of internal membrane was tenfold of surface outside membrane; The internal membrane is important than outside. The internal membrane formed organelle and provide a separating space for specific chemical or biological reaction. ...
... surrounding by membrane Biomembrane are bilayer structure. the area of internal membrane was tenfold of surface outside membrane; The internal membrane is important than outside. The internal membrane formed organelle and provide a separating space for specific chemical or biological reaction. ...
occasional article intracellular protein degradation: from a vague
... mammalian tissues are in a dynamic state. Moreover, our experiments have shown that the proteins of growing E. coli are static. Therefore it seems necessary to conclude that the synthesis and maintenance of proteins within growing cells is not necessarily or inherently associated with a ‘dynamic sta ...
... mammalian tissues are in a dynamic state. Moreover, our experiments have shown that the proteins of growing E. coli are static. Therefore it seems necessary to conclude that the synthesis and maintenance of proteins within growing cells is not necessarily or inherently associated with a ‘dynamic sta ...
Sp3 Represses Gene Expression via the Titration of Promoter
... members and other factors account for the induced transcription of additional Sp-dependent genes remains to be determined. Several years ago we identified two novel Sp3-derived proteins, termed M1 and M2, that arise by internal translational initiation within the region of Sp3 mRNA that encodes the ...
... members and other factors account for the induced transcription of additional Sp-dependent genes remains to be determined. Several years ago we identified two novel Sp3-derived proteins, termed M1 and M2, that arise by internal translational initiation within the region of Sp3 mRNA that encodes the ...
Protein Catabolism
... dipeptides and tripeptides. The free amino acids as well as the di and tripeptides are absorbed by the intestinal mucosa cells which subsequently are released into the blood stream where they are absorbed by other tissues. II. Turnover of Cellular Proteins Cellular proteins are continually being syn ...
... dipeptides and tripeptides. The free amino acids as well as the di and tripeptides are absorbed by the intestinal mucosa cells which subsequently are released into the blood stream where they are absorbed by other tissues. II. Turnover of Cellular Proteins Cellular proteins are continually being syn ...
A single mutation in the core domain of the lac repressor reduces
... when necessary. This metabolic switch is mediated by the lac repressor (LacI), which in the absence of lactose binds to the operator DNA sequence to inhibit transcription. Allosteric rearrangements triggered by binding of the lactose isomer allolactose to the core domain of the repressor impede DNA ...
... when necessary. This metabolic switch is mediated by the lac repressor (LacI), which in the absence of lactose binds to the operator DNA sequence to inhibit transcription. Allosteric rearrangements triggered by binding of the lactose isomer allolactose to the core domain of the repressor impede DNA ...
PROTEIN SYNTHESIS: TRANSLATION AND
... subunits, each of which contains RNA and many proteins. With one exception, each protein is present in a single copy per ribosome, as is each RNA species. The composition of major ribosome types is shown in Table 17.1, and characteristics of their RNAs are given in Table 16.1. Ribosome architecture ...
... subunits, each of which contains RNA and many proteins. With one exception, each protein is present in a single copy per ribosome, as is each RNA species. The composition of major ribosome types is shown in Table 17.1, and characteristics of their RNAs are given in Table 16.1. Ribosome architecture ...
Guideline on the Clinical Investigation of the Pharmacokinetics of
... immunoassays are able to detect structurally-related compounds, active or not, bioassays detect only active compounds, be they the parent product or its metabolites or any other structurally-related compounds, including endogenous proteins (see below). Due to the different characteristics of the met ...
... immunoassays are able to detect structurally-related compounds, active or not, bioassays detect only active compounds, be they the parent product or its metabolites or any other structurally-related compounds, including endogenous proteins (see below). Due to the different characteristics of the met ...
Dynamic in vivo interactions among Myc network members
... distinct roles for the encoded proteins in tissue-speci®c negative control of cellular proliferation (Foley et al., 1998; Schreiber-Agus et al., 1998). Despite the well-described interactions of Myc network proteins, little is known about their distribution within the nucleus, or how they seek out o ...
... distinct roles for the encoded proteins in tissue-speci®c negative control of cellular proliferation (Foley et al., 1998; Schreiber-Agus et al., 1998). Despite the well-described interactions of Myc network proteins, little is known about their distribution within the nucleus, or how they seek out o ...
Characterization of Acetyl-CoA Carboxylases in the Basal
... ACC mRNA quantification was consistent with the global transcript patterns in dinoflagellates ACC mRNA quantification was consistent with the global transcript patterns in dinoflagellates over a diel light cycle. Previously, in A. carterae, global changes in mRNA abundance were observed over a die ...
... ACC mRNA quantification was consistent with the global transcript patterns in dinoflagellates ACC mRNA quantification was consistent with the global transcript patterns in dinoflagellates over a diel light cycle. Previously, in A. carterae, global changes in mRNA abundance were observed over a die ...