Impact of glucose uptake rate on recombinant protein production in
... in lower growth rates and lower accumulation of acetic acid in comparison to the wild type. By using the mutants in batch cultivations, the formation of acetic acid to levels detrimental to cell growth could be avoided, and ten times higher cell density was reached. Thus, the use of the mutant strai ...
... in lower growth rates and lower accumulation of acetic acid in comparison to the wild type. By using the mutants in batch cultivations, the formation of acetic acid to levels detrimental to cell growth could be avoided, and ten times higher cell density was reached. Thus, the use of the mutant strai ...
Solutions
... • Integral membrane proteins that “carry” 1 or more small polar substances (monosaccharides, amino acids, nucleotides…) • The movement of only one substance across a membrane is called uniport • The movement of more than one substance across a membrane is called cotransport • change their shape betw ...
... • Integral membrane proteins that “carry” 1 or more small polar substances (monosaccharides, amino acids, nucleotides…) • The movement of only one substance across a membrane is called uniport • The movement of more than one substance across a membrane is called cotransport • change their shape betw ...
SUPPLEMENTARY INFORMATION TABLE OF CONTENTS: 1
... -ACG) as found in L. major. No tRNA gene was predicted upstream of the EM1 7SL RNA gene. Some snRNAs are not associated with a tRNA gene but are associated with a functional Box-B element. Further investigation would be required to reveal the presence of an upstream box B in EM1. Either way, the ...
... -ACG) as found in L. major. No tRNA gene was predicted upstream of the EM1 7SL RNA gene. Some snRNAs are not associated with a tRNA gene but are associated with a functional Box-B element. Further investigation would be required to reveal the presence of an upstream box B in EM1. Either way, the ...
Modes of Membrane Transport
... • Integral membrane proteins that “carry” 1 or more small polar substances (monosaccharides, amino acids, nucleotides…) • The movement of only one substance across a membrane is called uniport • The movement of more than one substance across a membrane is called cotransport • change their shape betw ...
... • Integral membrane proteins that “carry” 1 or more small polar substances (monosaccharides, amino acids, nucleotides…) • The movement of only one substance across a membrane is called uniport • The movement of more than one substance across a membrane is called cotransport • change their shape betw ...
Improving protein fold recognition with hybrid
... (Altschul et al., 1997), were followed by methods derived from the probabilistic interpretation of profiles using hidden Markov models (profile HMMs) (Krogh et al., 1994), like SAM-T98 (Karplus et al., 1998) or HMMER (Eddy, 1998), which improved both the alignment quality and the sensitivity/specifi ...
... (Altschul et al., 1997), were followed by methods derived from the probabilistic interpretation of profiles using hidden Markov models (profile HMMs) (Krogh et al., 1994), like SAM-T98 (Karplus et al., 1998) or HMMER (Eddy, 1998), which improved both the alignment quality and the sensitivity/specifi ...
Insulin mRNA to Protein Kit Student Handout
... When RNA polymerase initially transcribes the insulin gene into messenger RNA, two introns – totaling 966 additional nucleotides – are included in the precursor form of the insulin mRNA. These intron sequences are removed from the mRNA in a splicing reaction as the mRNA is being transported out of t ...
... When RNA polymerase initially transcribes the insulin gene into messenger RNA, two introns – totaling 966 additional nucleotides – are included in the precursor form of the insulin mRNA. These intron sequences are removed from the mRNA in a splicing reaction as the mRNA is being transported out of t ...
SFE1, a Constituent of the Fertilization Envelope
... and lift off the cell surface to form a stable, impervious, fertilization envelope. At least six major proteins are present in the envelope, and recently we have identified cDNA clones of two, ovoperoxidase, and SFE9. Here we report on the identification and characterization of SFE1, a constituent o ...
... and lift off the cell surface to form a stable, impervious, fertilization envelope. At least six major proteins are present in the envelope, and recently we have identified cDNA clones of two, ovoperoxidase, and SFE9. Here we report on the identification and characterization of SFE1, a constituent o ...
Fundamentals of Biochemistry. Life at the Molecular Level. 3rd Edition Brochure
... Brochure More information from http://www.researchandmarkets.com/reports/2242601/ ...
... Brochure More information from http://www.researchandmarkets.com/reports/2242601/ ...
Bovine Peptidoglycan Recognition Protein
... bovine PGRP-S under conditions approximating the extracellular milieu by modulating cations, ionic strength, osmolality, and serum concentration. The complete set of molecular targets for PGRPs has yet to be elucidated. Our previous results provided evidence that bPGRP-S could act in a PGN-independe ...
... bovine PGRP-S under conditions approximating the extracellular milieu by modulating cations, ionic strength, osmolality, and serum concentration. The complete set of molecular targets for PGRPs has yet to be elucidated. Our previous results provided evidence that bPGRP-S could act in a PGN-independe ...
Chapter 5- Structure and Function of Large Biological Molecules
... units follow. However, this analogy falls far short of describing the great diversity of macromolecules because most biological polymers are much longer than the longest word. Proteins, for example, are built from 20 kinds of amino acids arranged in chains that are typically hundreds of amino acids ...
... units follow. However, this analogy falls far short of describing the great diversity of macromolecules because most biological polymers are much longer than the longest word. Proteins, for example, are built from 20 kinds of amino acids arranged in chains that are typically hundreds of amino acids ...
template
... There are two competing approaches to attack protein-protein docking problem: i) Automated, ii) VR-based. Several research groups are working on automated docking [Morris et al. 1999; Hart and Read 2004] which can be reformulated as an optimization problem. The goal is to minimize binding energy of ...
... There are two competing approaches to attack protein-protein docking problem: i) Automated, ii) VR-based. Several research groups are working on automated docking [Morris et al. 1999; Hart and Read 2004] which can be reformulated as an optimization problem. The goal is to minimize binding energy of ...
Poster for RCPSC mee.. - University of Alberta
... Ceramide is a sphingolipid second messenger produced in response to cellular stress via activation of sphingomyelinases. Agonists that cause cellular production of ceramide include cytokines (TNF, Fas), agents of environmental stress (heat, UV irradiation), and chemotherapeutic agents. The accumulat ...
... Ceramide is a sphingolipid second messenger produced in response to cellular stress via activation of sphingomyelinases. Agonists that cause cellular production of ceramide include cytokines (TNF, Fas), agents of environmental stress (heat, UV irradiation), and chemotherapeutic agents. The accumulat ...
The anhidrotic ectodermal dysplasia gene (EDA) undergoes
... variants show different expression patterns in eight tissues analyzed, suggesting a regulatory mechanism for gene expression. The EDA-A form of the protein is transported to the cell membrane and induces rounding of the cells, properties also associated with the 135 amino acid isoform. We have deter ...
... variants show different expression patterns in eight tissues analyzed, suggesting a regulatory mechanism for gene expression. The EDA-A form of the protein is transported to the cell membrane and induces rounding of the cells, properties also associated with the 135 amino acid isoform. We have deter ...
Structural and Thermal Stability Characterization of Protein
... system and the lowest energy acceptor emission (rhodamine B) was observed as emission spectra. B) The emission spectra obtained after exciting at 650nm. Figure.2.1 A) C18SA modified Hemoglobin (Hb) with increasing ration of Hb:C18SA. Color change was observed with increase in the C18SA concentration ...
... system and the lowest energy acceptor emission (rhodamine B) was observed as emission spectra. B) The emission spectra obtained after exciting at 650nm. Figure.2.1 A) C18SA modified Hemoglobin (Hb) with increasing ration of Hb:C18SA. Color change was observed with increase in the C18SA concentration ...
Proteomics Analysis of Immunoprecipitated Proteins Associated with
... from 133 to 388 is the kinase domain which has signifycant sequence similarity with kinase domains from other MAP3Ks. The putative C-terminal domain consists of the residues 389 to 467. It has been shown that C-terminally truncated Tpl-2 (oncogenic form) is approximately five times more active in au ...
... from 133 to 388 is the kinase domain which has signifycant sequence similarity with kinase domains from other MAP3Ks. The putative C-terminal domain consists of the residues 389 to 467. It has been shown that C-terminally truncated Tpl-2 (oncogenic form) is approximately five times more active in au ...
Protein Measurement with the Folin Phenol Reagent
... alcohol and isopropyl ether, and dried. These samples dissolved very slowly in 2 ml. of 1 N NaOH. However, after standing overnight, the protein appeared to be nearly all dissolved and aliquots gave readings 97 per cent of those obtained with non-precipitated samples. Other samples were heated for 3 ...
... alcohol and isopropyl ether, and dried. These samples dissolved very slowly in 2 ml. of 1 N NaOH. However, after standing overnight, the protein appeared to be nearly all dissolved and aliquots gave readings 97 per cent of those obtained with non-precipitated samples. Other samples were heated for 3 ...
Cholesterol, steroids, and related molecules
... LDL receptor. This regulation is mediated by the transcription factor, SREBP (sterol response element binding protein). SREBP is produced as a membrane-bound protein attached to the endoplasmic reticulum of the cell via a second protein, SCAP. In cholesterol-starved cells SREBP:SCAP is transported ...
... LDL receptor. This regulation is mediated by the transcription factor, SREBP (sterol response element binding protein). SREBP is produced as a membrane-bound protein attached to the endoplasmic reticulum of the cell via a second protein, SCAP. In cholesterol-starved cells SREBP:SCAP is transported ...
8. Peptide bonds, polypeptides and proteins Polypeptide and
... regulatory sequences? The answer is proteins. The class of proteins that do this are known generically as transcription factors. Their shared property is that they bind with high affinity to specific sequences of nucleotides within DNA molecules. For historical reasons, in bacteria these transcripti ...
... regulatory sequences? The answer is proteins. The class of proteins that do this are known generically as transcription factors. Their shared property is that they bind with high affinity to specific sequences of nucleotides within DNA molecules. For historical reasons, in bacteria these transcripti ...
10 Phytohormones and Signal Transduction Pathways in Plants
... have been identified during the past decade. The amino acid permease-like gene AUX1 and the family of bacterial transporter-like PIN genes encode putative auxin influx (Bennett et al., 1996) and efflux carriers, respectively. Characterization of the first putative auxin efflux carrier PIN1 (Gälweile ...
... have been identified during the past decade. The amino acid permease-like gene AUX1 and the family of bacterial transporter-like PIN genes encode putative auxin influx (Bennett et al., 1996) and efflux carriers, respectively. Characterization of the first putative auxin efflux carrier PIN1 (Gälweile ...
Motor protein control of ion flux is an early step in embryonic left
... event.(2) Most importantly, a number of mechanisms appear to be required for LR patterning at pre-cilia stages. In the chick, a system of gap-junctional communication is required at stage 2–3 (prior to node formation) for correct LR asymmetry(20) and, in the frog, very early mechanisms include gap j ...
... event.(2) Most importantly, a number of mechanisms appear to be required for LR patterning at pre-cilia stages. In the chick, a system of gap-junctional communication is required at stage 2–3 (prior to node formation) for correct LR asymmetry(20) and, in the frog, very early mechanisms include gap j ...
us whey proteins in ready-to-drink beverages
... selecting a whey protein are a) the whey protein’s method of isolation which determines the composition of the WPC or the WPI and b) a consistent source and manufacturing process to produce the ingredient. The compositional differences between ion exchange and membrane filtered WPI are explained in ...
... selecting a whey protein are a) the whey protein’s method of isolation which determines the composition of the WPC or the WPI and b) a consistent source and manufacturing process to produce the ingredient. The compositional differences between ion exchange and membrane filtered WPI are explained in ...
U.S. WHEY PROTEINS IN READY-TO-DRINK BEVERAGES
... selecting a whey protein are a) the whey protein’s method of isolation which determines the composition of the WPC or the WPI and b) a consistent source and manufacturing process to produce the ingredient. The compositional differences between ion exchange and membrane filtered WPI are explained in ...
... selecting a whey protein are a) the whey protein’s method of isolation which determines the composition of the WPC or the WPI and b) a consistent source and manufacturing process to produce the ingredient. The compositional differences between ion exchange and membrane filtered WPI are explained in ...
Neisseria conserved hypothetical protein DMP12
... DNA mimic proteins are unique factors that control the DNA-binding activity of target proteins by directly occupying their DNA-binding sites. To date, only a few DNA mimic proteins have been reported and their functions analyzed. Here, we present evidence that the Neisseria conserved hypothetical pr ...
... DNA mimic proteins are unique factors that control the DNA-binding activity of target proteins by directly occupying their DNA-binding sites. To date, only a few DNA mimic proteins have been reported and their functions analyzed. Here, we present evidence that the Neisseria conserved hypothetical pr ...
Transport and Storage of Metal Ions in Biology
... an environment ®lled with metal ion chelators such as soluble proteins, peptides (e.g., glutathione), and organic metabolites (e.g., citrate). These molecules may impede the ability of metalloproteins to acquire their metal cofactor by acting as competitive chelators. In the case of Cu, speci®c solu ...
... an environment ®lled with metal ion chelators such as soluble proteins, peptides (e.g., glutathione), and organic metabolites (e.g., citrate). These molecules may impede the ability of metalloproteins to acquire their metal cofactor by acting as competitive chelators. In the case of Cu, speci®c solu ...