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`Hybrid Protein Model`for optimally defining 3D protein structure
`Hybrid Protein Model`for optimally defining 3D protein structure

... Although it has been suggested that protein structures may adopt only a limited number of folds (Govindarajan et al., 1999), the determination of their 3D structure from their sequence remains a difficult task (Baker and Sali, 2001). The conventional methods, such as homology modeling (Fiser and Sal ...
Two dileucine motifs mediate late endosomal/lysosomal targeting of
Two dileucine motifs mediate late endosomal/lysosomal targeting of

... TMEM192 (transmembrane protein 192) is a novel constituent of late endosomal/lysosomal membranes with four potential transmembrane segments and an unknown function that was initially discovered by organellar proteomics. Subsequently, localization in late endosomes/lysosomes has been confirmed for ov ...
Mitochondrial stress causes increased succination of proteins in
Mitochondrial stress causes increased succination of proteins in

... (1,1-dimethylbiguanide hydrochloride)], for a further 2–8 days (maturation period); during this time lipid droplets accumulated in the cytoplasm. Preliminary experiments using L-glucose as an osmotic control indicated that it had no effect on protein succination (results not shown). Culture medium w ...
1 - Columbia University
1 - Columbia University

... genetics allows investigators to eliminate specific proteins by ‘knocking out’ genes; to increase the concentrations of particular proteins by increasing the number of copies of the corresponding genes or by using a more active promoter on such genes; or to alter the function of a protein by introdu ...
Slide 1
Slide 1

... AS it is proved in past that machine learning techniques are elegant in classifying the biological data. Hau and sun applied the SVM for classification of prokaryotic and eukaryotic proteins and shown that it is better than statistical methods as well as other machine learning techniques such as AN ...
The Golgi Apparatus - Global Science Books
The Golgi Apparatus - Global Science Books

... Fig. 2 Fluorescent proteins are an important tool in visualising parts of the secretory pathway. Various proteins have been established as markers for different organelles in the secretory pathway. These micrographs demonstrate the usage of several markers alone or in combination to show the distrib ...
Discovering patterns to extract protein–protein interactions from full
Discovering patterns to extract protein–protein interactions from full

... full text have been largely ignored. In this paper, we propose a novel and robust method to discover patterns to extract protein interactions. It is based on dynamic programming (DP). In the realm of homology search between protein or DNA sequences, a global and local alignment algorithm has been th ...
From yeast to patient neurons and back again - Lindquist Lab
From yeast to patient neurons and back again - Lindquist Lab

... be resistant to high NAB2 concentrations. Second, we used a library of approximately 300,000 strains wherein each cell carried a single transposon randomly inserted into the yeast genome. These can disrupt open reading frames or regulatory elements to modulate expression. By selecting for rare varia ...
Protein Motif Recognition I Introduction
Protein Motif Recognition I Introduction

... the structure or fold of a protein provides the key to understanding its biological function, and proteins play a variety of important roles in the body (e.g., as enzymes, antibodies, etc.). Proteins may also be associated with particular human diseases, and thus, understanding protein structure may ...
Task - The British Association of Sport and Exercise Sciences
Task - The British Association of Sport and Exercise Sciences

... serves as a template. ...
Protein conformational changes induced by adsorption onto material
Protein conformational changes induced by adsorption onto material

... 1.3. Theoretical considerations on protein adsorption onto materials. Most biological phenomena occur in water, at an ionic concentration that considerably reduces the range of electrostatic interactions. As a result, proteins and materials interact by multiple short-range (Å) interactions (H-bondi ...
Dear teacher/student
Dear teacher/student

... different databases. When looking for proteins, the best databases are Swiss-Prot and Uniprot KB. Enter the name of the protein in the search bar. 2. You will probably end up with several hits. All proteins in this list are somehow related to the protein in your query. Use the description to determi ...
Lecture_14.pps
Lecture_14.pps

... Special Problems for N-Linked Sugar Chains • N-glycosylation occurs in ER-Topology for lysosomal degradation is wrong • ~50% of ER proteins misfold and are degraded what happens to the sugar chain? To glycopeptides? • Protein synthetic rate and glycosylation rate must be coordinated • Competition f ...
Expression profiles of Ciona tailbud embryos - Development
Expression profiles of Ciona tailbud embryos - Development

... the gene expression profiles we first selected C. intestinalis tailbud embryos, as we wish to re-examine more carefully the sequence data when we eventually obtain nearly all the saturated ESTs. The present analysis is therefore partial and will be described rather briefly. Sequences of the 5′-most ...
SNX9 – a prelude to vesicle release - Journal of Cell Science
SNX9 – a prelude to vesicle release - Journal of Cell Science

... at the neck of endocytic vesicles, as well as the role of SNX9 in coupling vesicle formation and actin remodeling. Finally, we discuss potential mechanisms of SNX9 regulation and outline a model of its function in CME. The SNX9-family proteins SNX9 was first described in 1999 as a Src homology 3 (SH ...
the enzymatic synthesis of protein. i.
the enzymatic synthesis of protein. i.

... quite insoluble in water, and exhibited the same solubility properties as plastein. The digest contained no active enzymes, bccause it had previously been boiled for an hour. It seems that the soluble protein on coming into contact with the solution of products at pH 4.0 was immediately denatured, o ...


... similar target promoter sequences: TTGACA near the -35 and TATAAT near the -10 elements (Helmann, 2002). These sigma factors are usually between 40 and 70 kDa in size and contain four conserved regions (Fig. 1) (Lonetto et al., 1992). These regions are based on sequence alignments and can be divided ...
Studying the pathogenesis of renal disease in cystinosis
Studying the pathogenesis of renal disease in cystinosis

... compounds – Alternative plasma membrane cystine transporter ...
Effect of
Effect of

... in goldfish protein requirements between these two studies may be attributed to the age of the fish. Other factors also affect dietary protein requirements, including the dietary protein to energy ratio, the essential amino acid composition, digestibility of the dietary protein, the dietary protein ...
Jane M. Carlton, , 207 (2007); DOI: 10.1126/science.1132894
Jane M. Carlton, , 207 (2007); DOI: 10.1126/science.1132894

... repeat sequences also hampered measurement of genome size, but we estimate it to be ~160 Mb (4). A core set of ~60,000 protein-coding genes was identified (Table 1), endowing T. vaginalis with one of the highest coding capacities among eukaryotes (table S2). Introns were identified in 65 genes, incl ...
protein
protein

... • Consequently, globular proteins are usually very soluble in aqueous solutions. • Most soluble proteins of the cell, such as the cytosolic enzymes, are globular in shape. • Membrane proteins are found in association with the various membrane systems of cells. • For interaction with the nonpolar ph ...
PDF version of paper
PDF version of paper

... The advantages of LATEX over WYSIWYG applications are well known1 . It has not only been the traditional application of choice to typeset mathematical formulae, but also had been employed to typeset music scores, games like crossword, chess and bridge. In this article, I will explain some of the too ...
Effective Scoring Function for Protein Sequence Design
Effective Scoring Function for Protein Sequence Design

... are added. ␹2 of Ser, Thr, and ␹3 of Tyr are assigned values of ⫺60°, 60°, and 180°. Three protonation states of His with the same expected frequencies are considered: N␦1 protonated, N⑀2 protonated, and both. ␹2 of Asn, His, and ␹3 of Gln are flipped 180° to make new rotamers to correct for the lack ...
Topology and Phosphorylation of Soybean Nodulin
Topology and Phosphorylation of Soybean Nodulin

... maintained between the plant and the microsymbiont. Biochemical and genetic evidence has shown that dicarboxylic acids are the primary carbon source supplied to the bacteroids by the plant (see Dilworth and Glenn, 1984). The flow of these compounds is controlled by the PBM. Several carbon and amino ...
Phylogeny of Geminiviruses - Journal of General Virology
Phylogeny of Geminiviruses - Journal of General Virology

... The taxonomy of viruses has improved greatly over the years as more information has become available about the physical and chemical nature of viruses. Early attempts at classifying viruses were based on characters such as symptomatology and host range which depended on the genotype of both viruses ...
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Magnesium transporter

This page links directly from the magnesium in biological systems page.Magnesium transporters are proteins that transport magnesium across the cell membrane. All forms of life require magnesium, yet the molecular mechanisms of Mg2+ uptake from the environment and the distribution of this vital element within the organism are only slowly being elucidated.In bacteria, Mg2+ is probably mainly supplied by the CorA protein and, where the CorA protein is absent, by the MgtE protein. In yeast the initial uptake is via the Alr1p and Alr2p proteins, but at this stage the only internal Mg2+ distributing protein identified is Mrs2p. Within the protozoa only one Mg2+ transporter (XntAp) has been identified. In metazoa, Mrs2p and MgtE homologues have been identified, along with two novel Mg2+ transport systems TRPM6/TRPM7 and PCLN-1. Finally, in plants, a family of Mrs2p homologues has been identified along with another novel protein, AtMHX.
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