TAP(Tandem Affinity Purification)
... and can create protein network maps • Analyzes more of the proteome • Works in membrane proteins • Works in many organisms ...
... and can create protein network maps • Analyzes more of the proteome • Works in membrane proteins • Works in many organisms ...
Fe Sparing and Fe Recycling Contribute to
... gene expression and metabolism. N, P, and K are major macronutrients and key components of fertilizer, whereas Cu, Fe, Mn, Zn, and other elements are classified as micronutrients, being required only in trace amounts in some cases. (The elemental symbols are used as abbreviations with no implication ...
... gene expression and metabolism. N, P, and K are major macronutrients and key components of fertilizer, whereas Cu, Fe, Mn, Zn, and other elements are classified as micronutrients, being required only in trace amounts in some cases. (The elemental symbols are used as abbreviations with no implication ...
Novel Riboswitch Ligand Analogs as Selective Inhibitors of Guanine
... riboswitches and therefore inhibit bacterial growth. Pyrimidine-based molecules that could fit into the guanine riboswitch aptamer binding site were selected based on molecular modeling of crystal structures [26,27] (Figure 2A). Using this approach, we identified two pyrimidine compounds 2,5,6triami ...
... riboswitches and therefore inhibit bacterial growth. Pyrimidine-based molecules that could fit into the guanine riboswitch aptamer binding site were selected based on molecular modeling of crystal structures [26,27] (Figure 2A). Using this approach, we identified two pyrimidine compounds 2,5,6triami ...
a study of intelligent techniques for protein secondary structure
... of known secondary and tertiary structures versus primary structures is relatively small. Although the secondary prediction started in the seventies but it has been together with the tertiary structure prediction a topic that is always under research. This paper presents a technical study on recent ...
... of known secondary and tertiary structures versus primary structures is relatively small. Although the secondary prediction started in the seventies but it has been together with the tertiary structure prediction a topic that is always under research. This paper presents a technical study on recent ...
The Role of Vacuole and Vacuolar H+
... investigate the roles of vacuolar H+-ATPase, plasma membrane H+-ATPase, and mitochondrial H+-ATPase in tolerance to cadmium, we examined the growth of yeast deletion mutants lacking different types of H+-ATPase in YPD media containing 80 μM of cadmium. Our results showed that a number of mutants lac ...
... investigate the roles of vacuolar H+-ATPase, plasma membrane H+-ATPase, and mitochondrial H+-ATPase in tolerance to cadmium, we examined the growth of yeast deletion mutants lacking different types of H+-ATPase in YPD media containing 80 μM of cadmium. Our results showed that a number of mutants lac ...
Characterization of the pscC
... bioRxiv preprint first posted online Aug. 26, 2016; doi: http://dx.doi.org/10.1101/071720. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license. ...
... bioRxiv preprint first posted online Aug. 26, 2016; doi: http://dx.doi.org/10.1101/071720. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license. ...
Nitrogen Balance and Protein Requirements: Definition and
... The Fate of Dietary Protein Nitrogen During the Postprandial Phase The diurnal cycle of feeding and fasting is accompanied by concurrent changes in protein turnover. Protein feeding is necessary to replenish the body protein stores that would be wasted during fasting [21–24]. Because of this, nitrog ...
... The Fate of Dietary Protein Nitrogen During the Postprandial Phase The diurnal cycle of feeding and fasting is accompanied by concurrent changes in protein turnover. Protein feeding is necessary to replenish the body protein stores that would be wasted during fasting [21–24]. Because of this, nitrog ...
Part 2 - OoCities
... predictor and yields an estimated percent similar to the experimental value. This is due to the fact the heteroatoms in penicillin G do not play a major role in making the molecule more hydrophilic. Although, the heteroatoms make up 62.5% of the molecule, the structure of penicillin reduces the hydr ...
... predictor and yields an estimated percent similar to the experimental value. This is due to the fact the heteroatoms in penicillin G do not play a major role in making the molecule more hydrophilic. Although, the heteroatoms make up 62.5% of the molecule, the structure of penicillin reduces the hydr ...
Regulation of the human vitamin C transporters expressed in COS
... to prevent the oxidation of L-ascorbic acid to the cell monolayer. At 30 min [uptake was directly proportional to time over a period of 60 min, as demonstrated previously (12)], the transport buffer was aspirated, and the cell monolayer was washed immediately with 1 ml of ice-cold buffer two times. ...
... to prevent the oxidation of L-ascorbic acid to the cell monolayer. At 30 min [uptake was directly proportional to time over a period of 60 min, as demonstrated previously (12)], the transport buffer was aspirated, and the cell monolayer was washed immediately with 1 ml of ice-cold buffer two times. ...
Differential protein profile in sexed bovine semen: shotgun
... found confirmation in studies on protein expression in sexed sperm cells.14,15 More recently, an extensive proteomic investigation of sexed sperm cells by 2-DE/MS shed light on differentially expressed proteins between bull X and Y spermatozoa including proteins involved in energy metabolism, cellul ...
... found confirmation in studies on protein expression in sexed sperm cells.14,15 More recently, an extensive proteomic investigation of sexed sperm cells by 2-DE/MS shed light on differentially expressed proteins between bull X and Y spermatozoa including proteins involved in energy metabolism, cellul ...
High-throughput knockout screen in Schizosaccharomyces pombe
... type (Fig. S2). Analysis of lys1-GFP dots in mature asci of strains carrying homozygous lys1-GFP indicated meiosis I homolog nondisjunction in dil1∆ mutant cells (Fig. 3A). To investigate chromosome segregation directly in anaphase I cells, we fixed cells and stained with antibodies against tubulin ...
... type (Fig. S2). Analysis of lys1-GFP dots in mature asci of strains carrying homozygous lys1-GFP indicated meiosis I homolog nondisjunction in dil1∆ mutant cells (Fig. 3A). To investigate chromosome segregation directly in anaphase I cells, we fixed cells and stained with antibodies against tubulin ...
Cloning and characterization of a phosphopantetheinyl transferase
... subtilis [20], and gramicidin in B. brevis [21], and polyketide biosynthesis, such as those for nystatin in Streptomyces noursei [22] and possibly landomycin in Streptomyces cyanogenus [23]. One could then envisage the use of known PPTase genes as additional probes for the cloning of novel polyketid ...
... subtilis [20], and gramicidin in B. brevis [21], and polyketide biosynthesis, such as those for nystatin in Streptomyces noursei [22] and possibly landomycin in Streptomyces cyanogenus [23]. One could then envisage the use of known PPTase genes as additional probes for the cloning of novel polyketid ...
PDF - Walter Lab
... incubation at room temperature, the dye concentrated into discrete foci, representing early endocytic intermediates17. Strikingly, all of the FM4-64-labelled foci localized to eisosomes (Fig. 3a), although not all eisosomes nucleated FM4-64 foci. Movies following FM4-64 uptake in living cells also s ...
... incubation at room temperature, the dye concentrated into discrete foci, representing early endocytic intermediates17. Strikingly, all of the FM4-64-labelled foci localized to eisosomes (Fig. 3a), although not all eisosomes nucleated FM4-64 foci. Movies following FM4-64 uptake in living cells also s ...
Non-human Primate Schlafen11 Inhibits Production of Both
... Schlafen11 is weak compared to that of some other primate versions of this protein, with the Schlafen11 proteins encoded by chimpanzee, orangutan, gibbon, and marmoset being particularly potent inhibitors of HIV-1 protein production. Interestingly, the strong inhibitors such as marmoset Schlafen11 c ...
... Schlafen11 is weak compared to that of some other primate versions of this protein, with the Schlafen11 proteins encoded by chimpanzee, orangutan, gibbon, and marmoset being particularly potent inhibitors of HIV-1 protein production. Interestingly, the strong inhibitors such as marmoset Schlafen11 c ...
Amino acids and proteins
... All groups capable of forming a hydrogen bond MUST, hence Hbonding in the backbone (C=O to N-H) by way of helices and sheets is an efficient way of ensuring maximum H-bonding. Sidechains can either accept (as in C=O) or donate (as in N-H, or OH) an H-bond. The capacity of proteins to form hydrogen b ...
... All groups capable of forming a hydrogen bond MUST, hence Hbonding in the backbone (C=O to N-H) by way of helices and sheets is an efficient way of ensuring maximum H-bonding. Sidechains can either accept (as in C=O) or donate (as in N-H, or OH) an H-bond. The capacity of proteins to form hydrogen b ...
The 92-kDa chitinase from Streptomyces olivaceoviridis contains a
... the ultraconcentrate of S. olivaceoviridis before [7] and more recently a serine proteinase of 22 kDa (Breves, unpublished) was characterized in our laboratory. We isolated 42-, 22- and 14-kDa proteinases by FPLC from the protein concentrate in the presence of Pefabloc SC. The proteins were blotted ...
... the ultraconcentrate of S. olivaceoviridis before [7] and more recently a serine proteinase of 22 kDa (Breves, unpublished) was characterized in our laboratory. We isolated 42-, 22- and 14-kDa proteinases by FPLC from the protein concentrate in the presence of Pefabloc SC. The proteins were blotted ...
Red blood cells: proteomics, physiology and metabolism
... exception of the highly lipophylic Aquaporin-3 pore. Two cation transporters were first identified by proteomics (splice isoform 4 of the Q04656 Copper-transporting ATPase 1 and the zinc transporter 1), while two different isoforms of calcium transporting ATPase, a ubiquitous form (isoform 1 or B) a ...
... exception of the highly lipophylic Aquaporin-3 pore. Two cation transporters were first identified by proteomics (splice isoform 4 of the Q04656 Copper-transporting ATPase 1 and the zinc transporter 1), while two different isoforms of calcium transporting ATPase, a ubiquitous form (isoform 1 or B) a ...
Protein Targeting to the Nuclear Pore. What Can
... Although these observations are unique to plants, it is difficult to test the function of putative plant NLS import factors biochemically because they are not depleted from the permeabilized protoplasts. Another unique feature of the plant import process is that NLS protein import can occur at 4°C ( ...
... Although these observations are unique to plants, it is difficult to test the function of putative plant NLS import factors biochemically because they are not depleted from the permeabilized protoplasts. Another unique feature of the plant import process is that NLS protein import can occur at 4°C ( ...
Type and timing of protein feeding to optimize anabolism
... increase (even moderate) of blood free amino acid levels. This was the case when subjects were fed casein or frequent small meals [10]. The main target in this situation appears to be in the splanchnic area for both protein degradation and for protein synthesis. In humans, it is likely that the inhi ...
... increase (even moderate) of blood free amino acid levels. This was the case when subjects were fed casein or frequent small meals [10]. The main target in this situation appears to be in the splanchnic area for both protein degradation and for protein synthesis. In humans, it is likely that the inhi ...
Overcoming stalled translation in human mitochondria
... of ICT1 must be carefully controlled with an as yet undefined specificity. The current hypothesis is that ICT1 must function in ribosome rescue but whether this occurs at stalling events within ORFs or on truncated transcripts is not yet clear. ICT1 does, however, have a bacterial homolog, YaeJ, which ...
... of ICT1 must be carefully controlled with an as yet undefined specificity. The current hypothesis is that ICT1 must function in ribosome rescue but whether this occurs at stalling events within ORFs or on truncated transcripts is not yet clear. ICT1 does, however, have a bacterial homolog, YaeJ, which ...
Chapter 1 - Utrecht University Repository
... CI-MPR. The receptor is recycled to the trans Golgi network (TGN) and the ligand is transported to lysosomes. Although the name early endosome implies it to be a single compartment, this clearly is an oversimplification. This was initially demonstrated by Maxfield and coworkers in Chinese hamster ov ...
... CI-MPR. The receptor is recycled to the trans Golgi network (TGN) and the ligand is transported to lysosomes. Although the name early endosome implies it to be a single compartment, this clearly is an oversimplification. This was initially demonstrated by Maxfield and coworkers in Chinese hamster ov ...
a review of the BLAST meeting, ï - Bacterial Locomotion and Signal
... liquids versus on surfaces. Likewise, a successful adaptive mechanism must sense the proper signals, which are as diverse as the environments occupied by bacteria and which range from small chemicals to specific wavelengths of light. In fact, what makes this field so exciting is the range and divers ...
... liquids versus on surfaces. Likewise, a successful adaptive mechanism must sense the proper signals, which are as diverse as the environments occupied by bacteria and which range from small chemicals to specific wavelengths of light. In fact, what makes this field so exciting is the range and divers ...