Transcriptional activation by the Antennapedia and fushi tarazu proteins in cultured Drosophila cells. Cell 57, 1017-30. pdf
... ily detected in cells transfected with the FS305 plasmid, but in contrast to the full-length protein the FS305 protein was found at high levels in both the cytoplasm and the nuclei (Figure 2~). When an An@ protein-producing plasmid was cotransfected with the Ubx reporter plasmid, the full-length An@ ...
... ily detected in cells transfected with the FS305 plasmid, but in contrast to the full-length protein the FS305 protein was found at high levels in both the cytoplasm and the nuclei (Figure 2~). When an An@ protein-producing plasmid was cotransfected with the Ubx reporter plasmid, the full-length An@ ...
Usha`s project - The University of Texas at Dallas
... Information) has over 12 million entries on sequence data. The main reason is due to the technical difficulties involved in structure determination. The structure of the proteins can be determined experimentally by using NMR and X-Ray crystallography. In NMR spectroscopy, radio waves and magnetic wa ...
... Information) has over 12 million entries on sequence data. The main reason is due to the technical difficulties involved in structure determination. The structure of the proteins can be determined experimentally by using NMR and X-Ray crystallography. In NMR spectroscopy, radio waves and magnetic wa ...
Monosaccharide transporters in plants: structure, function and
... Homologous transporter genes characterized in Escherichia coli or in Saccharomyces cerevisiae are usually expressed in response to the availability of certain monosaccharides in the growth medium. These genes encode proteins with relatively narrow substrate speci¢cities, such as the S. cerevisiae ga ...
... Homologous transporter genes characterized in Escherichia coli or in Saccharomyces cerevisiae are usually expressed in response to the availability of certain monosaccharides in the growth medium. These genes encode proteins with relatively narrow substrate speci¢cities, such as the S. cerevisiae ga ...
X-ray structure of the C-terminal domain of a coronavirus nucle
... nucleocapsid protein was expressed as before (Zhou, Williams et al. 1996). The protein was further purified by heparin affinity chromatography, concentrated to 1-2 mg/ml and was checked for monodispersity by dynamic light scattering (Dynapro) and negative stain electron microscopy. Limited proteolyt ...
... nucleocapsid protein was expressed as before (Zhou, Williams et al. 1996). The protein was further purified by heparin affinity chromatography, concentrated to 1-2 mg/ml and was checked for monodispersity by dynamic light scattering (Dynapro) and negative stain electron microscopy. Limited proteolyt ...
The Use of Multiple Transcription Starts Causes the Dual Targeting
... used to determine the transcription starts. 1RC and 2RC are the forward primers designed at the oligonucleotides that were copied from oligoribonucleotides artificially attached to replace the 5¢ cap structure (see Materials and Methods). (C) The amplified products were run on a 4% GTG Nusieve Agaro ...
... used to determine the transcription starts. 1RC and 2RC are the forward primers designed at the oligonucleotides that were copied from oligoribonucleotides artificially attached to replace the 5¢ cap structure (see Materials and Methods). (C) The amplified products were run on a 4% GTG Nusieve Agaro ...
Bioinformatics of proteins: Sequence, structure and the `symbiosis
... • A collaborative effort to address the need for consistent descriptions of gene products in different database • The GO project has developed three structured controlled vocabularies (ontologies) that describe gene products in terms of their associated biological processes, cellular components and ...
... • A collaborative effort to address the need for consistent descriptions of gene products in different database • The GO project has developed three structured controlled vocabularies (ontologies) that describe gene products in terms of their associated biological processes, cellular components and ...
The Electrophoretic Movement of Proteins from Various
... with moderate success. For examples of this type of characterization see Hesseltine, Benedict & Pridham (1954), Pridham, Hesseltine & Benedict (1958), Gottlieb (1959), Pridham (1964), Pridham & Lyons (1956). Nevertheless, there is still an urgent need for new criteria that might aid in the recogniti ...
... with moderate success. For examples of this type of characterization see Hesseltine, Benedict & Pridham (1954), Pridham, Hesseltine & Benedict (1958), Gottlieb (1959), Pridham (1964), Pridham & Lyons (1956). Nevertheless, there is still an urgent need for new criteria that might aid in the recogniti ...
Biological membranes - Essays in Biochemistry
... Synthesis of membrane proteins In eukaryotic cells, the synthesis of membrane proteins destined for the plasma membrane, ER or any other membrane-bound compartment begins on cytosolic ribosomes. After a short segment of protein has been synthesized, the ribosome, mRNA and nascent protein chain assoc ...
... Synthesis of membrane proteins In eukaryotic cells, the synthesis of membrane proteins destined for the plasma membrane, ER or any other membrane-bound compartment begins on cytosolic ribosomes. After a short segment of protein has been synthesized, the ribosome, mRNA and nascent protein chain assoc ...
... bacterial strain, and an RBS (prokaryotic ribosome binding sequence). Cell-free systems (eukaryotic [e.g., TNT® systems] and prokaryotic [e.g., S30 systems]) are fast, amenable to high-throughput, and often used for pull-down experiments and enzymatic assays (2,3); these systems require a bacterioph ...
Plant stress adaptations — making metabolism move Hans J
... of mRNA transcript and protein levels or accelerated turnover of components of detoxification systems have been shown [13••,16,17]. To some extent, the transgenic enhancement of ROS scavenging components has been shown to positively affect plant performance during stress [16–18], but protection has ...
... of mRNA transcript and protein levels or accelerated turnover of components of detoxification systems have been shown [13••,16,17]. To some extent, the transgenic enhancement of ROS scavenging components has been shown to positively affect plant performance during stress [16–18], but protection has ...
Protein hydrolysates in sports nutrition
... Collectively, these studies strongly suggest that ingestion of a fast-acting protein hydrolysate and/or amino acid supplement results a less efficient uptake by the splanchnic bed and therefore increases the magnitude of the acute increase in amino acids in the systemic circulation that are availab ...
... Collectively, these studies strongly suggest that ingestion of a fast-acting protein hydrolysate and/or amino acid supplement results a less efficient uptake by the splanchnic bed and therefore increases the magnitude of the acute increase in amino acids in the systemic circulation that are availab ...
AtPex14p maintains peroxisomal functions by determining protein
... (Nishimura et al., 1996). Glyoxysomes, leaf peroxisomes and unspecialized peroxisomes are known to be converted into one another under certain conditions (Nishimura et al., 1996). For example, glyoxysomes in etiolated cotyledons are transformed directly into leaf peroxisomes during the greening of c ...
... (Nishimura et al., 1996). Glyoxysomes, leaf peroxisomes and unspecialized peroxisomes are known to be converted into one another under certain conditions (Nishimura et al., 1996). For example, glyoxysomes in etiolated cotyledons are transformed directly into leaf peroxisomes during the greening of c ...
Heart Failure and Protein Quality Control
... Recent data indicate that polyubiquitin chain assembly factor (E4) may also play an important role,16 as UFD2a, an E4 exclusively expressed in cardiac muscle during mouse embryonic development, is essential for normal heart development.17 There is 1 ubiquitin E1, approximately 50 E2s, and more than ...
... Recent data indicate that polyubiquitin chain assembly factor (E4) may also play an important role,16 as UFD2a, an E4 exclusively expressed in cardiac muscle during mouse embryonic development, is essential for normal heart development.17 There is 1 ubiquitin E1, approximately 50 E2s, and more than ...
Planctomycetes and eukaryotes: A case of analogy not homology
... measure such as that implemented in the basic local alignment search tool (BLAST) suite of programs [50]. Our default position is that, if the level of similarity between two sequences is significantly higher than the chance expectation, then this is most likely to be a consequence of shared ancestr ...
... measure such as that implemented in the basic local alignment search tool (BLAST) suite of programs [50]. Our default position is that, if the level of similarity between two sequences is significantly higher than the chance expectation, then this is most likely to be a consequence of shared ancestr ...
Prediction of DNA-binding residues in proteins from amino acid
... 2007; Ho et al., 2007; Kuznetsov et al., 2006; Ofran et al., 2007; Wang and Brown, 2006a, b). In the present work, we aim to design optimal predictors for DNA-binding sites in proteins directly from amino acid sequences. In designing classifiers, the following points are taken into consideration: (i ...
... 2007; Ho et al., 2007; Kuznetsov et al., 2006; Ofran et al., 2007; Wang and Brown, 2006a, b). In the present work, we aim to design optimal predictors for DNA-binding sites in proteins directly from amino acid sequences. In designing classifiers, the following points are taken into consideration: (i ...
- Wiley Online Library
... small scale high throughput in mammalian systems (Hacker et al., 2009) or in baculovirus infected insect cells (Brown et al., 2011; Hunt, 2005; Rieffel et al., 2014). Nevertheless, this remains a timeintensive baculovirus based system without direct in-line analysis of the expressed protein. A secon ...
... small scale high throughput in mammalian systems (Hacker et al., 2009) or in baculovirus infected insect cells (Brown et al., 2011; Hunt, 2005; Rieffel et al., 2014). Nevertheless, this remains a timeintensive baculovirus based system without direct in-line analysis of the expressed protein. A secon ...
ppt - Chair of Computational Biology
... which in this simple example determines drug response: allele C results in a therapeutic response, whereas allele G results in an adverse reaction. In this example, the selection of just one SNP from each of the groups A–E would be sufficient to fully represent all of the haplotype diversity. Each h ...
... which in this simple example determines drug response: allele C results in a therapeutic response, whereas allele G results in an adverse reaction. In this example, the selection of just one SNP from each of the groups A–E would be sufficient to fully represent all of the haplotype diversity. Each h ...
Prolyl isomerases are important determinants of intracellular pH
... In a constantly changing environment, organisms have developed different strategies to maintain their internal media relatively constant. These mechanisms are known generically as homeostatic processes and allow the organism to maintain internal conditions within a tolerable range (Gao et al., 2004) ...
... In a constantly changing environment, organisms have developed different strategies to maintain their internal media relatively constant. These mechanisms are known generically as homeostatic processes and allow the organism to maintain internal conditions within a tolerable range (Gao et al., 2004) ...
1Memstruc
... B. increasing the number of double bonds in the fatty acids C. decreasing the amount of cholesterol in the membrane D. increasing the temperature of the membrane 2. Which one of the following statements correctly describes membrane structure? A. Most membrane constituents are fixed in a specific loc ...
... B. increasing the number of double bonds in the fatty acids C. decreasing the amount of cholesterol in the membrane D. increasing the temperature of the membrane 2. Which one of the following statements correctly describes membrane structure? A. Most membrane constituents are fixed in a specific loc ...