Next-generation proteomics: towards an integrative view of
... interconnectivity of proteins into complexes and signalling networks that are highly divergent in time and space. In recent years, proteomics technologies — particularly mass spectrometry (MS)-based protein identification — have matured immensely through cumulative technological advances in instrume ...
... interconnectivity of proteins into complexes and signalling networks that are highly divergent in time and space. In recent years, proteomics technologies — particularly mass spectrometry (MS)-based protein identification — have matured immensely through cumulative technological advances in instrume ...
Mitochondrial dysfunction in neurodevelopmental disorders
... affecting mitochondria might cause PD. Other mitochondrial toxins characterized as parkinsonism-inducing reagents include 6-OHDA, rotenone, and paraquat. Studies of animal models of PD induced with these toxins suggest that mitochondrial dysfunction and oxidative stress are important pathogenic mech ...
... affecting mitochondria might cause PD. Other mitochondrial toxins characterized as parkinsonism-inducing reagents include 6-OHDA, rotenone, and paraquat. Studies of animal models of PD induced with these toxins suggest that mitochondrial dysfunction and oxidative stress are important pathogenic mech ...
EC->PDB
... evolutionary relationships between proteins of known structure. The database has been constructed using a combination of manual inspection and automated methods, because current automatic sequence and structure comparison tools cannot identify all structural relationships reliably. Proteins are clas ...
... evolutionary relationships between proteins of known structure. The database has been constructed using a combination of manual inspection and automated methods, because current automatic sequence and structure comparison tools cannot identify all structural relationships reliably. Proteins are clas ...
Lipid–protein interactions probed by electron crystallography
... studies [17]. In general, overall B-factors were found to be lower in the electron crystallographic structures than those obtained by X-ray crystallography. Importantly, AQP0 residues that in 2D crystals interacted with lipids were found to interact with detergent molecules in 3D crystals used for ...
... studies [17]. In general, overall B-factors were found to be lower in the electron crystallographic structures than those obtained by X-ray crystallography. Importantly, AQP0 residues that in 2D crystals interacted with lipids were found to interact with detergent molecules in 3D crystals used for ...
NO 3
... 2. NITROGEN FIXING BACTERIA (Rhizobium) found in the roots of some plants (leguminous). The rhizobium use an enzyme called nitrogenase which converts N2 gas into ammonium ions NH4+. They can only do this if they have 1). a supply of N2, 2). A supply of ATP 3).anaerobic conditions (no oxygen). 3. Fix ...
... 2. NITROGEN FIXING BACTERIA (Rhizobium) found in the roots of some plants (leguminous). The rhizobium use an enzyme called nitrogenase which converts N2 gas into ammonium ions NH4+. They can only do this if they have 1). a supply of N2, 2). A supply of ATP 3).anaerobic conditions (no oxygen). 3. Fix ...
Gene Expression Profiling During Asexual Development of the Late
... detectable, hybridizations were performed using RNA from 89 tissues. These included the developmental stages addressed by this article (germinated and ungerminated asexual spores, plus hyphae), other stages such as oosporogenesis (Prakob and Judelson 2007), and miscellaneous chemical treatments. Of ...
... detectable, hybridizations were performed using RNA from 89 tissues. These included the developmental stages addressed by this article (germinated and ungerminated asexual spores, plus hyphae), other stages such as oosporogenesis (Prakob and Judelson 2007), and miscellaneous chemical treatments. Of ...
Electrophoretic Extraction and Proteomic Characterization of
... of particulate nitrogen in marine sediments [5–8]. In addition to proteins representing the largest fraction of organic nitrogen, within the unique amino acid sequence, proteins also can provide functional and phylogenetic information on the organisms from which they were produced, potentially makin ...
... of particulate nitrogen in marine sediments [5–8]. In addition to proteins representing the largest fraction of organic nitrogen, within the unique amino acid sequence, proteins also can provide functional and phylogenetic information on the organisms from which they were produced, potentially makin ...
Cloning, Purification, and Partial Characterization of the
... and its integration site [5,6] clearly suggest that it is an intein. The intein is inserted in the middle of the Walker-A motif [7], a highly conserved motif which is found in all members of the AAA+ family of ATPases [8,9]. The sequence of the motif in Halobacterium MCM is GDPGTGKS and the intein i ...
... and its integration site [5,6] clearly suggest that it is an intein. The intein is inserted in the middle of the Walker-A motif [7], a highly conserved motif which is found in all members of the AAA+ family of ATPases [8,9]. The sequence of the motif in Halobacterium MCM is GDPGTGKS and the intein i ...
Whey Protein: A Functional Food
... Bland, J.S., Costarella, L., Levin, B. et al. (2004). Clinical Nutrition – A Functional Approach. 2nd ed. Gig Harbour: The Institute for Functional Medicine Gahr, M., Speer, C.P., Damerau, B. et al., 1991. Influence of lactoferrin on the function of human polymorphonuclear leukocytes and monocytes. ...
... Bland, J.S., Costarella, L., Levin, B. et al. (2004). Clinical Nutrition – A Functional Approach. 2nd ed. Gig Harbour: The Institute for Functional Medicine Gahr, M., Speer, C.P., Damerau, B. et al., 1991. Influence of lactoferrin on the function of human polymorphonuclear leukocytes and monocytes. ...
the peroxisomal endomembrane system and the role of the ER
... This is in contrast to COPI in cultured human fibroblasts, in which peroxisome-to-ER retrograde protein transport, if any, does not depend on COPI (South et al., 2000; Voorn-Brouwer et al., 2001). These findings further support the notion that yeast and higher eukaryotic organisms may use different ...
... This is in contrast to COPI in cultured human fibroblasts, in which peroxisome-to-ER retrograde protein transport, if any, does not depend on COPI (South et al., 2000; Voorn-Brouwer et al., 2001). These findings further support the notion that yeast and higher eukaryotic organisms may use different ...
Protein profiling of the dimorphic, pathogenic fungus,Penicillium
... dimorphic member of the genus. Dimorphism results from a process, termed phase transition, which is regulated by temperature of incubation. At room temperature, the fungus grows filamentously (mould phase), but at body temperature (37°C), a uninucleate yeast form develops that reproduces by fission. ...
... dimorphic member of the genus. Dimorphism results from a process, termed phase transition, which is regulated by temperature of incubation. At room temperature, the fungus grows filamentously (mould phase), but at body temperature (37°C), a uninucleate yeast form develops that reproduces by fission. ...
Stress signaling from the lumen of the endoplasmic reticulum
... phenobarbitol) are induced to meet their demand. Overproduction of microsomal cytochrome P450Alk1 (P450Alk1) of Candida maltosa in S. cerevisiae activates the UPR to proliferate the ER extensively (Takewaka et al. 1999). Cells that are selected for resistance to cholesterol-lowering drugs that inhib ...
... phenobarbitol) are induced to meet their demand. Overproduction of microsomal cytochrome P450Alk1 (P450Alk1) of Candida maltosa in S. cerevisiae activates the UPR to proliferate the ER extensively (Takewaka et al. 1999). Cells that are selected for resistance to cholesterol-lowering drugs that inhib ...
The families of pathogenesis-related proteins, their activities, and
... were identified as chitinases [45] and β-1,3-glucanases [38] with potential antifungal activity, it has often been suggested that the collective set of PRs may be effective in inhibiting pathogen growth, multiplication and\or spread, and be responsible for the state of SAR [42, 65]. Originally, five ...
... were identified as chitinases [45] and β-1,3-glucanases [38] with potential antifungal activity, it has often been suggested that the collective set of PRs may be effective in inhibiting pathogen growth, multiplication and\or spread, and be responsible for the state of SAR [42, 65]. Originally, five ...
Lipid transfer and metabolism across the endolysosomal
... and LEs/lysosomes are implicated in the regulation of signaling processes [26], in organelle maturation and dynamics [27,28] and possibly also in lipid exchange. In fact, LEs/lysosomes represent the main source of extracellular-derived cholesterol (mainly in the form of low-density lipoproteins, LDL ...
... and LEs/lysosomes are implicated in the regulation of signaling processes [26], in organelle maturation and dynamics [27,28] and possibly also in lipid exchange. In fact, LEs/lysosomes represent the main source of extracellular-derived cholesterol (mainly in the form of low-density lipoproteins, LDL ...
Microsoft PowerPoint - plasma membrane notes
... polar, so they repel water (hydrophobic); found on the inside of the membrane; fats, oxygen, carbon dioxide, alcohol and urea can move through this layer (non polar molecules) •Non-polar because: Cholesterol: Membrane Proteins: •Two types: •Function and structure of glycocalyx ...
... polar, so they repel water (hydrophobic); found on the inside of the membrane; fats, oxygen, carbon dioxide, alcohol and urea can move through this layer (non polar molecules) •Non-polar because: Cholesterol: Membrane Proteins: •Two types: •Function and structure of glycocalyx ...
RNA binding proteins: a common denominator of neuronal function
... In eukaryotic cells, gene activity is not directly reflected by protein levels because mRNA processing, transport, stability, and translation are co- and post-transcriptionally regulated. These processes, collectively known as the ribonome, are tightly controlled and carried out by a plethora of tra ...
... In eukaryotic cells, gene activity is not directly reflected by protein levels because mRNA processing, transport, stability, and translation are co- and post-transcriptionally regulated. These processes, collectively known as the ribonome, are tightly controlled and carried out by a plethora of tra ...
mRNA Transport in Yeast: Time to Reinvestigate the
... the mRNA that is not transported from the nucleus is nevertheless polyadenylated and sufficiently stable and abundant to be detected by in situ hybridization (Figure 2). These screens yielded 17 complementation groups of recessive mutants defective in mRNA transport (mtr; Kadowaki et al., 1994a) and ...
... the mRNA that is not transported from the nucleus is nevertheless polyadenylated and sufficiently stable and abundant to be detected by in situ hybridization (Figure 2). These screens yielded 17 complementation groups of recessive mutants defective in mRNA transport (mtr; Kadowaki et al., 1994a) and ...
Thermodynamic analysis of the unfolding and stability of the dimeric
... to prevent DNA duplex melting [7] has also been described. HU proteins from several species of bacillus growing in environments of different temperatures have already been isolated and studied [4,7,15–18]. The close sequence homology among them suggests that their native structure must be very simil ...
... to prevent DNA duplex melting [7] has also been described. HU proteins from several species of bacillus growing in environments of different temperatures have already been isolated and studied [4,7,15–18]. The close sequence homology among them suggests that their native structure must be very simil ...
Anti-c-myc antibody 9E10 - Protein Engineering, Design and Selection
... higher affinity than KLISEEDL, but, as expected, decreased affinity to 9E10 compared to the original myc-tag EQKLISEEDLN, as did the 10-mer QKLISEFELN. However, the 11-mer EQKLISEFELN bound to the antibody somewhat better than the original myc-tag. In addition, a peptide with randomly chosen amino a ...
... higher affinity than KLISEEDL, but, as expected, decreased affinity to 9E10 compared to the original myc-tag EQKLISEEDLN, as did the 10-mer QKLISEFELN. However, the 11-mer EQKLISEFELN bound to the antibody somewhat better than the original myc-tag. In addition, a peptide with randomly chosen amino a ...
Translation | Principles of Biology from Nature Education
... The ribosome keeps the mRNA and tRNA close to each other and brings the next amino acid to the carboxyl end of the growing polypeptide. Without the ribosome, the hydrogen bonding between the tRNA and mRNA would be too weak to hold it there long enough for a peptide bond to form. The ribosome catalyz ...
... The ribosome keeps the mRNA and tRNA close to each other and brings the next amino acid to the carboxyl end of the growing polypeptide. Without the ribosome, the hydrogen bonding between the tRNA and mRNA would be too weak to hold it there long enough for a peptide bond to form. The ribosome catalyz ...
Gene Section TMPRSS2 (transmembrane protease, serine 2) Atlas of Genetics and Cytogenetics
... TMPRSS2 is a 492 amino acid single-pass type II membrane protein. It contains a Serine protease domain (aa 255-492) of the S1 family, followed by a Scavenger receptor cysteine-rich domain (SRDR, aa 149-242) of group A; an LDL receptor class A (LDLRA, aa 113-148) domain forms a binding site for calci ...
... TMPRSS2 is a 492 amino acid single-pass type II membrane protein. It contains a Serine protease domain (aa 255-492) of the S1 family, followed by a Scavenger receptor cysteine-rich domain (SRDR, aa 149-242) of group A; an LDL receptor class A (LDLRA, aa 113-148) domain forms a binding site for calci ...