1Memstruc
... B. increasing the number of double bonds in the fatty acids C. decreasing the amount of cholesterol in the membrane D. increasing the temperature of the membrane 2. Which one of the following statements correctly describes membrane structure? A. Most membrane constituents are fixed in a specific loc ...
... B. increasing the number of double bonds in the fatty acids C. decreasing the amount of cholesterol in the membrane D. increasing the temperature of the membrane 2. Which one of the following statements correctly describes membrane structure? A. Most membrane constituents are fixed in a specific loc ...
Statistical Selection of Amino Acids Fortifying a Minimal Defined
... In addition, the use of minimal defined media might be more cost-effective than using complex rich media due to the lower medium costs with no requirement for the removal of unknown complex compounds during downstream processes [16]. However, complex media based on yeast extract and peptone have bee ...
... In addition, the use of minimal defined media might be more cost-effective than using complex rich media due to the lower medium costs with no requirement for the removal of unknown complex compounds during downstream processes [16]. However, complex media based on yeast extract and peptone have bee ...
Amino Acid Starter Kit
... Protein, your students explored the primary and tertiary structure of proteins, including the role of the amino acid sidechains in determining the overall shape of a protein. The second set of activities in the kit explores the secondary structure of proteins. Simple tools allow students to explore: ...
... Protein, your students explored the primary and tertiary structure of proteins, including the role of the amino acid sidechains in determining the overall shape of a protein. The second set of activities in the kit explores the secondary structure of proteins. Simple tools allow students to explore: ...
Genetic Analysis of the Yeast Cytoskeleton.
... fusion proteins or synthetic peptides. The alternate procedure(plan B) begins with proteins identified biochemically as part of the cytoskeleton. The gene can be obtained by standard cloning methods, and mutations can be produced in the gene in vitro. In yeast, these mutantgenes can then be substitu ...
... fusion proteins or synthetic peptides. The alternate procedure(plan B) begins with proteins identified biochemically as part of the cytoskeleton. The gene can be obtained by standard cloning methods, and mutations can be produced in the gene in vitro. In yeast, these mutantgenes can then be substitu ...
P1 and P2 protein heterodimer binding to the P0 protein of
... structure undergoes cyclical conformational changes that affect the interaction of P0 with the P1/P2 heterodimers, thereby facilitating the release of the acidic proteins. The CTD of the stalk acidic proteins exhibits a significant mobility, which has hindered the elucidation of the crystal structure ...
... structure undergoes cyclical conformational changes that affect the interaction of P0 with the P1/P2 heterodimers, thereby facilitating the release of the acidic proteins. The CTD of the stalk acidic proteins exhibits a significant mobility, which has hindered the elucidation of the crystal structure ...
Arabidopsis Contains Ancient Classes of Differentially Expressed
... tissue types and pollen, and unlike any conventional actin, AtARP2 was expressed at very low levels. Thus, it seemed possible that AtARP2 and other subclasses of plant ARPs might each be contained in gene families with different members showing complementary expression patterns with activity in more ...
... tissue types and pollen, and unlike any conventional actin, AtARP2 was expressed at very low levels. Thus, it seemed possible that AtARP2 and other subclasses of plant ARPs might each be contained in gene families with different members showing complementary expression patterns with activity in more ...
8679821 - Southern Illinois University System
... adding bactericidal yeast expressing at least one antibacterial protein of the invention to an environment at risk of bacterial contamination. Another method of the invention includes adding bactericidal yeast expressing at least one antibacterial protein of the invention to a batch solution at risk ...
... adding bactericidal yeast expressing at least one antibacterial protein of the invention to an environment at risk of bacterial contamination. Another method of the invention includes adding bactericidal yeast expressing at least one antibacterial protein of the invention to a batch solution at risk ...
Secretion of Bacillus subtilis a-Amylase in the Periplasmic Space of
... al., 1983). This plasmid was designated pTUB4 and the DNA sequence of the inserted 2.3 kb fragment is known (Ohmura et al., 1983). pYK33 I(a 'Psi vector') is a derivative of pBR322 containing the same multiple cloning sites as M13mplO (Norrander et al., 1983) at the end of the promoter-signal sequen ...
... al., 1983). This plasmid was designated pTUB4 and the DNA sequence of the inserted 2.3 kb fragment is known (Ohmura et al., 1983). pYK33 I(a 'Psi vector') is a derivative of pBR322 containing the same multiple cloning sites as M13mplO (Norrander et al., 1983) at the end of the promoter-signal sequen ...
Modifications of the E.coli Lac repressor for expression in eukaryotic
... Inducers such as allolactose (the natural substrate of betagalactosidase) or isopropyl-beta-D-thiogalactopyranoside (IPTG, a gratuitous synthetic inducer) bind to the repressor and cause a conformational change which effectively decreases the affinity of the repressor for the operator. The approxima ...
... Inducers such as allolactose (the natural substrate of betagalactosidase) or isopropyl-beta-D-thiogalactopyranoside (IPTG, a gratuitous synthetic inducer) bind to the repressor and cause a conformational change which effectively decreases the affinity of the repressor for the operator. The approxima ...
Active Transport Lab
... (that is, toward a region of higher concentration). By altering ATP concentrations, you will be able to speed or slow the operation of the ATP-driven sodium/potassium pump and explore the consequences for amino acid transport. Similarly, you can alter the cellular (inside) or extracellular (outside ...
... (that is, toward a region of higher concentration). By altering ATP concentrations, you will be able to speed or slow the operation of the ATP-driven sodium/potassium pump and explore the consequences for amino acid transport. Similarly, you can alter the cellular (inside) or extracellular (outside ...
SCit: web tools for protein side chain
... the proteins, which can result in incorrect encoding. (iii) It detects if there are missing atoms in the side chains. In such cases, the side chains are automatically rebuilt in a standard conformation corresponding to the most probable conformation of the side chain in a backbone-independent rotame ...
... the proteins, which can result in incorrect encoding. (iii) It detects if there are missing atoms in the side chains. In such cases, the side chains are automatically rebuilt in a standard conformation corresponding to the most probable conformation of the side chain in a backbone-independent rotame ...
Application Note #14 - GE Healthcare Life Sciences
... produced the sequence Val17-Gly-Ser-Asn-Val-, Val being at position 17 of the HM2 chain. Cleavage at position 47 with trypsin followed by RP-HPLC separation of the two resulting peptides yielded a fragment whose sequence was found to match the C-terminal sequence from positions 48 to 64. Finally, ma ...
... produced the sequence Val17-Gly-Ser-Asn-Val-, Val being at position 17 of the HM2 chain. Cleavage at position 47 with trypsin followed by RP-HPLC separation of the two resulting peptides yielded a fragment whose sequence was found to match the C-terminal sequence from positions 48 to 64. Finally, ma ...
Residue-specific Mass Signatures for the
... containing 0.1% Tween-20, followed by three times washes with PBS. The washed samples were resuspended in a 2× protein loading buffer, boiled for 10 min, and subjected to 18% SDS-PAGE for protein separation. The protein bands were visualized by silver stain, and the band corresponding to H2A.X was c ...
... containing 0.1% Tween-20, followed by three times washes with PBS. The washed samples were resuspended in a 2× protein loading buffer, boiled for 10 min, and subjected to 18% SDS-PAGE for protein separation. The protein bands were visualized by silver stain, and the band corresponding to H2A.X was c ...
The enduracidin biosynthetic gene cluster from
... ORFs and is located within a 116 kb genetic locus that was fully sequenced. Targeted disruption of non-ribosomal peptide synthetase (NRPS) genes in the cluster abolished enduracidin production and confirmed function. The cluster includes four genes, endA-D, encoding two-, seven-, eight- and one-modu ...
... ORFs and is located within a 116 kb genetic locus that was fully sequenced. Targeted disruption of non-ribosomal peptide synthetase (NRPS) genes in the cluster abolished enduracidin production and confirmed function. The cluster includes four genes, endA-D, encoding two-, seven-, eight- and one-modu ...
BIOINFORMATICS ORIGINAL PAPER Going from where to why—interpretable prediction of protein subcellular localization
... thus used to draw conclusions about its cellular role, interaction partners and function in biological processes. During the past decade, huge number of novel proteins were discovered in the context of large-scale sequencing projects. Unfortunately, for a majority of these proteins their subcellular ...
... thus used to draw conclusions about its cellular role, interaction partners and function in biological processes. During the past decade, huge number of novel proteins were discovered in the context of large-scale sequencing projects. Unfortunately, for a majority of these proteins their subcellular ...
Characterization of a novel phosphatidylinositol 3
... are numbered on the right and amino acid residues on the left. The two partial cDNAs identified in the original two-hybrid screen with PDK1 encode residues 57–362 and 116–362. ...
... are numbered on the right and amino acid residues on the left. The two partial cDNAs identified in the original two-hybrid screen with PDK1 encode residues 57–362 and 116–362. ...
Chapter 5 Macromolecules
... Concept 5.3: Lipids are a diverse group of hydrophobic molecules • Lipids are the one class of large biological molecules that do not form polymers • The unifying feature of lipids is having little or no affinity for water • Lipids are hydrophobic because they consist mostly of hydrocarbons, which ...
... Concept 5.3: Lipids are a diverse group of hydrophobic molecules • Lipids are the one class of large biological molecules that do not form polymers • The unifying feature of lipids is having little or no affinity for water • Lipids are hydrophobic because they consist mostly of hydrocarbons, which ...
Effects of Aging on Activities of Mitochondrial Electron Transport
... significantly in both old (to 90±4 % of adult, p<0.05) and senescent rats (to 83±2 % of adult, p<0.01). The most pronounced age-related changes were observed in KCNsensitive complex IV (COX) activity, comparing to adult the activity decreased to 79±3 % (p<0.01) in old and 63±4 % (p<0.001) in senesce ...
... significantly in both old (to 90±4 % of adult, p<0.05) and senescent rats (to 83±2 % of adult, p<0.01). The most pronounced age-related changes were observed in KCNsensitive complex IV (COX) activity, comparing to adult the activity decreased to 79±3 % (p<0.01) in old and 63±4 % (p<0.001) in senesce ...
The Strategic Use of Ruminally Protected Amino Acids in Dairy
... percentage (Bucholtz et al., 2008). In another experiment with similar results (Benefield et al., 2006) it appeared that because of higher than expected DMI, estimated gram requirements were in excess, and this may have been the reason for the failure, but it certainly was not the result of low MET ...
... percentage (Bucholtz et al., 2008). In another experiment with similar results (Benefield et al., 2006) it appeared that because of higher than expected DMI, estimated gram requirements were in excess, and this may have been the reason for the failure, but it certainly was not the result of low MET ...
Genomic Tagging of the Anaphase-Promoting Complex Activator
... G1 phase. Cells in this condition are virtually undistinguishable from cells in senescence (8). The fact that this effect of Tax can only be observed in transformed cells (and, incidentally, in S. cerevisiae cells) makes it an important topic in cancer research. Although Tax-induced senescence is ph ...
... G1 phase. Cells in this condition are virtually undistinguishable from cells in senescence (8). The fact that this effect of Tax can only be observed in transformed cells (and, incidentally, in S. cerevisiae cells) makes it an important topic in cancer research. Although Tax-induced senescence is ph ...
Protein Import, Replication, and Inheritance of a Vestigial
... set of proteins required for the biogenesis of an endosymbiosis-derived organelle. Giardia intestinalis, often described as the earliest branching eukaryote, contains double membrane-bounded structures involved in ironsulfur cluster biosynthesis, an essential function of mitochondria. Here we presen ...
... set of proteins required for the biogenesis of an endosymbiosis-derived organelle. Giardia intestinalis, often described as the earliest branching eukaryote, contains double membrane-bounded structures involved in ironsulfur cluster biosynthesis, an essential function of mitochondria. Here we presen ...
Specific Growth Inhibition by Acetate of an Escherichia coli Strain
... (March et al., 1988; Chen et al., 1990). Era is both cytosolic and associated with membrane and autophosphorylated in a GTP-dependent manner (Lin et al., 1994; Sood et al., 1994). Era was originally found in E. coli (Ahnn et al., 1986) and has been shown to be highly conserved not only in prokaryote ...
... (March et al., 1988; Chen et al., 1990). Era is both cytosolic and associated with membrane and autophosphorylated in a GTP-dependent manner (Lin et al., 1994; Sood et al., 1994). Era was originally found in E. coli (Ahnn et al., 1986) and has been shown to be highly conserved not only in prokaryote ...