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... at position 152. These all show an unusually large 140Å Cα displacement. Crystal contacts as a cause may be ruled out since the mutant structure has been solved in a number of different space groups, including the same space group as the wild-type, and all structures give an identical Cα displacemen ...
... at position 152. These all show an unusually large 140Å Cα displacement. Crystal contacts as a cause may be ruled out since the mutant structure has been solved in a number of different space groups, including the same space group as the wild-type, and all structures give an identical Cα displacemen ...
13059_2010_2366_MOESM1_ESM
... degradation was only localized to the path of infection. Other results of the carbon source utilization experiment are consistent with the results from the annotation: no growth was obtained when xylan was used as a carbon source, confirming the absence of xylan degrading enzymes, while the presence ...
... degradation was only localized to the path of infection. Other results of the carbon source utilization experiment are consistent with the results from the annotation: no growth was obtained when xylan was used as a carbon source, confirming the absence of xylan degrading enzymes, while the presence ...
Nedd8 processing enzymes inSchizosaccharomyces pombe
... The final candidate we examined as a potential Nedd8 processing enzyme in fission yeast was csn5+. This subunit of the COP9/signalosome complex is well described as a metalloprotease capable of deconjugating Nedd8 from its cullin substrates [25,26]. Indeed, as observed before [21,26], hyperneddylati ...
... The final candidate we examined as a potential Nedd8 processing enzyme in fission yeast was csn5+. This subunit of the COP9/signalosome complex is well described as a metalloprotease capable of deconjugating Nedd8 from its cullin substrates [25,26]. Indeed, as observed before [21,26], hyperneddylati ...
THE DETERMINATION OF PROTEIN IN CEREBROSPINAL FLUID
... cc. of 20 per cent trichloracetic acid. Mix. Heat in a boiling water bath for from 0.5 to one minute. Let cool. Add 6 cc. of absolute methyl alcohol. Mix. Centrifuge. Decant supernatant fluid and drain for 5 minutes on a towel. Washing appears to be unnecessary. B. Oxidation. Add 2 cc. of the dilute ...
... cc. of 20 per cent trichloracetic acid. Mix. Heat in a boiling water bath for from 0.5 to one minute. Let cool. Add 6 cc. of absolute methyl alcohol. Mix. Centrifuge. Decant supernatant fluid and drain for 5 minutes on a towel. Washing appears to be unnecessary. B. Oxidation. Add 2 cc. of the dilute ...
CHAPTER 3 STRUCTURAL ELEMENTS OF
... 1. helix-loop-helix: it is present in many Ca2+ binding proteins (calmodulin, parvalbumin, troponin C ) or DNA binding proteins 2. β hairpin : two antiparallel β-strands are kept together by a short loop of 2-5 residues. 3. greek key: four β strands (minimum), two short loops and one long loop are n ...
... 1. helix-loop-helix: it is present in many Ca2+ binding proteins (calmodulin, parvalbumin, troponin C ) or DNA binding proteins 2. β hairpin : two antiparallel β-strands are kept together by a short loop of 2-5 residues. 3. greek key: four β strands (minimum), two short loops and one long loop are n ...
Developmental and functional studies of the SLC12 gene family
... early 1980s (13), long before it was realized that they were genetically related proteins. The first ...
... early 1980s (13), long before it was realized that they were genetically related proteins. The first ...
Protein and Peptides Drug delivery, PEGylations and Biotech based products, Immunomodulated molecules
... It can produce a decrease in the in-vitro activity of proteins, but generally this negative effect is offset in the biological systems by increased half-life It lacks immunogenecity, but antibodies to PEG are generated in rabbits; only if PEG is combined with highly immunogenic proteins No one ...
... It can produce a decrease in the in-vitro activity of proteins, but generally this negative effect is offset in the biological systems by increased half-life It lacks immunogenecity, but antibodies to PEG are generated in rabbits; only if PEG is combined with highly immunogenic proteins No one ...
Synapse Specificity Minireview and Long
... induced in the first pathway generates new proteins that can then be used by the synapses stimulated in the second pathway to generate late LTP. Because the second pathway was only transiently treated with a protein synthesis inhibitor, it is also possible that new mRNAs, rather than proteins, are t ...
... induced in the first pathway generates new proteins that can then be used by the synapses stimulated in the second pathway to generate late LTP. Because the second pathway was only transiently treated with a protein synthesis inhibitor, it is also possible that new mRNAs, rather than proteins, are t ...
Prediction of mitochondrial proteins of malaria parasite
... homologous sequences from the benchmark dataset, a cutoff threshold of 25% was imposed to exclude those proteins from the benchmark datasets that have equal to or greater than 25% sequence identity to any other in a same subset (Chou and Shen 2006b; Chou and Shen 2007a, b; Chou and Shen 2008a). Howe ...
... homologous sequences from the benchmark dataset, a cutoff threshold of 25% was imposed to exclude those proteins from the benchmark datasets that have equal to or greater than 25% sequence identity to any other in a same subset (Chou and Shen 2006b; Chou and Shen 2007a, b; Chou and Shen 2008a). Howe ...
GEFs: master regulators of G
... example, Rho-family GEFs Vav and Tiam contain PH domains directly C-terminal to their GEF domains. These phosphoinositolbinding domains might not only function as membrane localization modules, but might also potentiate the GEF activity of the neighboring DH domain. In any case, PH domain-containing ...
... example, Rho-family GEFs Vav and Tiam contain PH domains directly C-terminal to their GEF domains. These phosphoinositolbinding domains might not only function as membrane localization modules, but might also potentiate the GEF activity of the neighboring DH domain. In any case, PH domain-containing ...
Channel-mediated lactic acid transport: a novel function for
... Most of the known bacterial AQP-type isoforms have been identified in Gram-negative bacteria, whereas most of the GLP-type sequences have been found in Gram-positive ones. This asymmetry might be related to the different structures and diffusion properties of the membranes and cell walls in the two ...
... Most of the known bacterial AQP-type isoforms have been identified in Gram-negative bacteria, whereas most of the GLP-type sequences have been found in Gram-positive ones. This asymmetry might be related to the different structures and diffusion properties of the membranes and cell walls in the two ...
Bioinorganic motifs: towards functional classification of metalloproteins
... (i) In coordination chemistry, the atoms or chemical groups bound to the central atom (usually a metal) via dative bond are called ligands. The donors of one or more electron pairs to the central atom are called monodentate or polydentate ligands, respectively. In bioinorganic chemistry, the ligands ...
... (i) In coordination chemistry, the atoms or chemical groups bound to the central atom (usually a metal) via dative bond are called ligands. The donors of one or more electron pairs to the central atom are called monodentate or polydentate ligands, respectively. In bioinorganic chemistry, the ligands ...
Investigation of a Zα-like Peptide Motif in Koi Herpesvirus
... A titration with poly(dGdC) B-DNA was conducted for both the ORF112 Y84A and N62D mutants using identical concentrations and the same procedure. In Figure 4, the conformational change in B-DNA with titration of ORF112 Y84A can be seen. Initial addition of protein causes an immediate inversion of CD ...
... A titration with poly(dGdC) B-DNA was conducted for both the ORF112 Y84A and N62D mutants using identical concentrations and the same procedure. In Figure 4, the conformational change in B-DNA with titration of ORF112 Y84A can be seen. Initial addition of protein causes an immediate inversion of CD ...
gfp_exercise_ver5
... 5 Using the cDNA sequence provided in this exercise, you estimate GFP’s mRNA length. In your laboratory, you then isolate total GFP RNA from jellyfish and resolve it on a gel based on the RNA size difference. You find two different GFP RNAs: one of the RNAs is bigger than your estimate and ...
... 5 Using the cDNA sequence provided in this exercise, you estimate GFP’s mRNA length. In your laboratory, you then isolate total GFP RNA from jellyfish and resolve it on a gel based on the RNA size difference. You find two different GFP RNAs: one of the RNAs is bigger than your estimate and ...
Paper
... channel formation by HlyA in lipid bilayer membranes did not require the presence of calcium ions, and the channel-forming activity of HlyA remained unaltered upon deletion of the RTX repeats or removal of free calcium ions (Döbereiner et al, 1996; Ludwig et al, 1988; Basler et al, 2007). This repre ...
... channel formation by HlyA in lipid bilayer membranes did not require the presence of calcium ions, and the channel-forming activity of HlyA remained unaltered upon deletion of the RTX repeats or removal of free calcium ions (Döbereiner et al, 1996; Ludwig et al, 1988; Basler et al, 2007). This repre ...
PHL 424 1st SF
... Newborn infants, especially if premature, lack an effective glucuronic acid conjugation mechanism for the degradation & detoxification of chloramphenicol, with subsequent inadequate renal excretion of unconjugated drug Consequently, when infants are given dosages above 50 mg/kg/d, the drug may accum ...
... Newborn infants, especially if premature, lack an effective glucuronic acid conjugation mechanism for the degradation & detoxification of chloramphenicol, with subsequent inadequate renal excretion of unconjugated drug Consequently, when infants are given dosages above 50 mg/kg/d, the drug may accum ...
Identification and Developmental Expression of a Novel Low
... The second was a polyclonal antibody made in rabbit against human glial fibrillary acidic protein (aGFAP) (Raff et al., 1979). This antiserum has been shown to cross-react with two intermediate filament proteins of X. laevis glial cells (Szaro and Gainer, 1988b). Western blots. Protein samples enric ...
... The second was a polyclonal antibody made in rabbit against human glial fibrillary acidic protein (aGFAP) (Raff et al., 1979). This antiserum has been shown to cross-react with two intermediate filament proteins of X. laevis glial cells (Szaro and Gainer, 1988b). Western blots. Protein samples enric ...
Muneeswaran
... 2006), ATCOECIS (Vandepoele et al. 2009), ATTED-II (Obayashi et al. 2007, 2009), CressExpress (Srinivasasainagendra et al. 2008), CSB.DB (Steinhauser et al. 2004), PRIME (Akiyama et al. 2008) and Plant Gene Expression Database (Horan et al, 2008) also useful in identifying the similarity between gen ...
... 2006), ATCOECIS (Vandepoele et al. 2009), ATTED-II (Obayashi et al. 2007, 2009), CressExpress (Srinivasasainagendra et al. 2008), CSB.DB (Steinhauser et al. 2004), PRIME (Akiyama et al. 2008) and Plant Gene Expression Database (Horan et al, 2008) also useful in identifying the similarity between gen ...
Engineering and Identifying Supercharged Proteins
... extranuclear space (McNaughton et al., 2009). We have probed the requirements for þ36 GFP uptake to better understand the mechanism by which it achieves such potent internalization. During internalization assays, the treated cells can be washed several times with heparin, a highly sulfated glycosami ...
... extranuclear space (McNaughton et al., 2009). We have probed the requirements for þ36 GFP uptake to better understand the mechanism by which it achieves such potent internalization. During internalization assays, the treated cells can be washed several times with heparin, a highly sulfated glycosami ...
Adaptive Silver Films for Detection of Antibody Binding
... the formation of aggregates of closely spaced silver particles covered with proteins, as opposed to the relatively dispersed particles of the initial film before protein deposition. Depending on the mass thickness of the initial film, small or large fractal-like aggregates can be formed. The small a ...
... the formation of aggregates of closely spaced silver particles covered with proteins, as opposed to the relatively dispersed particles of the initial film before protein deposition. Depending on the mass thickness of the initial film, small or large fractal-like aggregates can be formed. The small a ...
Protein Folding Cell and Mol Biology Lab
... click here). The animation shows that amino acids move as new weak bonds form between the protein and the substrate. It is found on my cell bio web page. We will also view the active site of "carb A" and see the motion of the R groups as they bind to the substrate with the program called KINEMAGE or ...
... click here). The animation shows that amino acids move as new weak bonds form between the protein and the substrate. It is found on my cell bio web page. We will also view the active site of "carb A" and see the motion of the R groups as they bind to the substrate with the program called KINEMAGE or ...
MB207_10 - MB207Jan2010
... Membrane lipids form a fluid bilayer that permits lateral diffusion of membrane lipids as well as proteins. → not being fixed in place with the membrane. Membrane fluidity changes with temperature. → decreasing as the temperature drops and increasing as it rises. → the change in the state of the mem ...
... Membrane lipids form a fluid bilayer that permits lateral diffusion of membrane lipids as well as proteins. → not being fixed in place with the membrane. Membrane fluidity changes with temperature. → decreasing as the temperature drops and increasing as it rises. → the change in the state of the mem ...
Homology Modeling via Protein Threading - lmm
... is not fully understood. The primary sequence may not fully specify the tertiary structure. chaperones ...
... is not fully understood. The primary sequence may not fully specify the tertiary structure. chaperones ...