Career of Tom Muir
... ■ in vitro screening of combinatorial peptide libraries ■ Characterize protein-protein interactions that regulate Abl function ...
... ■ in vitro screening of combinatorial peptide libraries ■ Characterize protein-protein interactions that regulate Abl function ...
Tps1 regulates the pentose phosphate pathway, nitrogen
... samples of mycelium following 48 hr growth in liquid complete media at 26 oC. RNA extraction from mycelium followed the method of Timberlake (1980). Total cell protein was extracted from known amounts of dry mycelium using a modification of the method described by Cove (1966). Following grinding of ...
... samples of mycelium following 48 hr growth in liquid complete media at 26 oC. RNA extraction from mycelium followed the method of Timberlake (1980). Total cell protein was extracted from known amounts of dry mycelium using a modification of the method described by Cove (1966). Following grinding of ...
Silk-inspired polymers and proteins
... from the repetitive motif found in silaffin proteins that are involved in silica formation at neutral pH in in vitro studies) were processed into fibres or films. Incubation of these materials with a water-soluble silicic acid species led to biomineralization of their surface [58]. Likewise, chimaer ...
... from the repetitive motif found in silaffin proteins that are involved in silica formation at neutral pH in in vitro studies) were processed into fibres or films. Incubation of these materials with a water-soluble silicic acid species led to biomineralization of their surface [58]. Likewise, chimaer ...
Chapter 1 - Utrecht University Repository
... CI-MPR. The receptor is recycled to the trans Golgi network (TGN) and the ligand is transported to lysosomes. Although the name early endosome implies it to be a single compartment, this clearly is an oversimplification. This was initially demonstrated by Maxfield and coworkers in Chinese hamster ov ...
... CI-MPR. The receptor is recycled to the trans Golgi network (TGN) and the ligand is transported to lysosomes. Although the name early endosome implies it to be a single compartment, this clearly is an oversimplification. This was initially demonstrated by Maxfield and coworkers in Chinese hamster ov ...
The Calcium-Binding Activity of a Vacuole
... sites. The levels of calcium binding proteins may have a significant impact on signaling processes and may regulate second messenger transmission (Camacho and Lechleiter, 1995; Mery et al., 1996; Coppolino et al., 1997). In an alternative role, calcium-dependent interactions of calnexin and calretic ...
... sites. The levels of calcium binding proteins may have a significant impact on signaling processes and may regulate second messenger transmission (Camacho and Lechleiter, 1995; Mery et al., 1996; Coppolino et al., 1997). In an alternative role, calcium-dependent interactions of calnexin and calretic ...
Predicted function of the vaccinia virus G5R protein
... The VACV G5R protein sequence was used as input in the Robetta protein structure prediction server (Chivian et al., 2003; Rohl et al., 2004; Chivian et al., 2005; Kim et al., 2004). Robetta is a fully automated structure prediction server that uses comparative modeling methods to model protein struc ...
... The VACV G5R protein sequence was used as input in the Robetta protein structure prediction server (Chivian et al., 2003; Rohl et al., 2004; Chivian et al., 2005; Kim et al., 2004). Robetta is a fully automated structure prediction server that uses comparative modeling methods to model protein struc ...
Chapter 1 Non-Canonical Amino Acids in Protein Engineering
... auxotrophic for both isoleucine and valine, were evaluated as expression hosts for the generation of proteins containing Tfv. The wild-type aaRS activity in these cells was insufficient for production of proteins containing Tfv, but incorporation of one of the diastereomers of Tfv (2S,3R-Tfv) into t ...
... auxotrophic for both isoleucine and valine, were evaluated as expression hosts for the generation of proteins containing Tfv. The wild-type aaRS activity in these cells was insufficient for production of proteins containing Tfv, but incorporation of one of the diastereomers of Tfv (2S,3R-Tfv) into t ...
learning objectives exam iii
... Be able to calculate turnover number for proteins containing either a single or several active sites. Understand the significance of catalytic efficiency Understand the derivation and utility of the Lineweaver-Burke plot in analyzing kinetic data from enzymatic reactions. Understand the different t ...
... Be able to calculate turnover number for proteins containing either a single or several active sites. Understand the significance of catalytic efficiency Understand the derivation and utility of the Lineweaver-Burke plot in analyzing kinetic data from enzymatic reactions. Understand the different t ...
Rab cascades and tethering factors in the endomembrane system
... is responsible for generating stable, Rab5 positive endosomal structures. Upon membrane recruitment, Rab5 gets activated by the Rabex-5 exchange factor. Rab5-GTP is then able to interact with its effector Rabaptin5, which forms a complex with Rabex-5. Interestingly, the effector stimulates the excha ...
... is responsible for generating stable, Rab5 positive endosomal structures. Upon membrane recruitment, Rab5 gets activated by the Rabex-5 exchange factor. Rab5-GTP is then able to interact with its effector Rabaptin5, which forms a complex with Rabex-5. Interestingly, the effector stimulates the excha ...
Proteins - virtual laboratories
... As you might remember from chemistry, carbon atoms (C) form four bonds, and where these are all single bonds, the basic structure of the atoms bound to a C is tetrahedral. We can think of an amino acid as a (highly) modified form of methane (CH4), with the C referred to as the alpha carbon (Cα). Ins ...
... As you might remember from chemistry, carbon atoms (C) form four bonds, and where these are all single bonds, the basic structure of the atoms bound to a C is tetrahedral. We can think of an amino acid as a (highly) modified form of methane (CH4), with the C referred to as the alpha carbon (Cα). Ins ...
Protein Composition of a High-Protein Barley Flour and Barley Grain
... (Konzak 1977, Lasztity 1984). This is similar to the results of this study for the partly dehulled barley grain. The distribution of the proteins among the different fractions, according to their solubility, is also known to depend on the variety, cultivation condition (Ldsztity 1984), and the fract ...
... (Konzak 1977, Lasztity 1984). This is similar to the results of this study for the partly dehulled barley grain. The distribution of the proteins among the different fractions, according to their solubility, is also known to depend on the variety, cultivation condition (Ldsztity 1984), and the fract ...
Phosphoproteomic analysis of Arabidopsis thaliana Hanna Klang Årstrand
... have such high level of sequence identity that it is unlikely that any gene-specific peptides would be found: S4, S13, S17, S26, S27, L15, L35a, L37 and L29 (Carroll et al. 2008; Byrne 2009). The presence of so many multigene families indicates that the protein composition of the ribosome could be d ...
... have such high level of sequence identity that it is unlikely that any gene-specific peptides would be found: S4, S13, S17, S26, S27, L15, L35a, L37 and L29 (Carroll et al. 2008; Byrne 2009). The presence of so many multigene families indicates that the protein composition of the ribosome could be d ...
Subtype-specific regulation of equilibrative nucleoside transporters
... sites, has been identified, to date, in human cells [8]. Protein kinase CK2 is a serine/threonine protein kinase that is found in nearly all compartments of mammalian cells [11], and exists as a heterotetramer composed of two catalytic (α) subunits and two regulatory (β) subunits [12]. It appears to ...
... sites, has been identified, to date, in human cells [8]. Protein kinase CK2 is a serine/threonine protein kinase that is found in nearly all compartments of mammalian cells [11], and exists as a heterotetramer composed of two catalytic (α) subunits and two regulatory (β) subunits [12]. It appears to ...
Plastid-Targeting Peptides from the
... only partially translocated across the ER membrane, so the mature peptide rests on the cytosolic face of the Golgi vesicles. The outer-most membrane of chlorarachniophyte and apicomplexan plastids is also not detectably contiguous with the host endomembrane, but the route taken by plastid proteins i ...
... only partially translocated across the ER membrane, so the mature peptide rests on the cytosolic face of the Golgi vesicles. The outer-most membrane of chlorarachniophyte and apicomplexan plastids is also not detectably contiguous with the host endomembrane, but the route taken by plastid proteins i ...
Divalent Metal Ions in Plant Mitochondria and Their Role in
... mitochondrial compartments was measured by ICPMS (Table II). In total mitochondrial samples, a significant reduction in Fe content was observed, and this loss was localized to the soluble fraction and not the integral membrane protein fraction. This apparent release of Fe from the soluble compartmen ...
... mitochondrial compartments was measured by ICPMS (Table II). In total mitochondrial samples, a significant reduction in Fe content was observed, and this loss was localized to the soluble fraction and not the integral membrane protein fraction. This apparent release of Fe from the soluble compartmen ...
The Psp system of Mycobacterium tuberculosis integrates envelope
... direction of transcription. The arrow PclgR indicates sequences upstream of clgR expressing promoter activity. IG = intergenic regions of indicated length, in bp. B. Effect of clgR deletion on gene stress response. Mid-log cultures of wild-type, clgR deletion mutant and complemented strains were tre ...
... direction of transcription. The arrow PclgR indicates sequences upstream of clgR expressing promoter activity. IG = intergenic regions of indicated length, in bp. B. Effect of clgR deletion on gene stress response. Mid-log cultures of wild-type, clgR deletion mutant and complemented strains were tre ...
Intrinsically Disordered Domains of the B Cell Receptor
... nearly 30% of the proteome is comprised of MPs, only 1% of all deposited structures in the protein data bank (PDB) are MPs. This is largely due to the inherent difficulties associated with working with this class of proteins [3]. Cell membranes are permeable barriers that maintain and protect the in ...
... nearly 30% of the proteome is comprised of MPs, only 1% of all deposited structures in the protein data bank (PDB) are MPs. This is largely due to the inherent difficulties associated with working with this class of proteins [3]. Cell membranes are permeable barriers that maintain and protect the in ...
Chemical Nature of the Amino Acids
... side chains and are therefore negatively charged at physiological pH (around neutral). The strongly polar nature of these residues means that they are most often found on the surface of globular proteins where they can interact favourably with solvent molecules. These residues can also take part in ...
... side chains and are therefore negatively charged at physiological pH (around neutral). The strongly polar nature of these residues means that they are most often found on the surface of globular proteins where they can interact favourably with solvent molecules. These residues can also take part in ...
Protein methylation at the surface and buried deep
... Ste14 (membrane-bound methyltransferase) a ...
... Ste14 (membrane-bound methyltransferase) a ...
Functional studies of the BTB domain in the Drosophila GAGA and
... Trithorax-like (trl) and mod(mdg4) are two Drosophila genes that encode BTB domain-containing proteins with roles in transcription regulation (25,26). trl encodes GAGA protein (26). GAGA was first identified as a factor that stimulates in vitro transcription of the Drosophila engrailed and Ultrabith ...
... Trithorax-like (trl) and mod(mdg4) are two Drosophila genes that encode BTB domain-containing proteins with roles in transcription regulation (25,26). trl encodes GAGA protein (26). GAGA was first identified as a factor that stimulates in vitro transcription of the Drosophila engrailed and Ultrabith ...
Comment in GO: This term is intended to
... macromolecular complex (GO:0032991) – A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which the constituent parts function together. protein complex (GO:0043234) – Any macromolecular complex composed of two or more polypeptide subunits, whic ...
... macromolecular complex (GO:0032991) – A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which the constituent parts function together. protein complex (GO:0043234) – Any macromolecular complex composed of two or more polypeptide subunits, whic ...
CHAPTER FOUR Pseudomonas aeruginosa INFLUENCED PLANKTONIC AND BIOFILM POPULATIONS BASED UPON
... sessile multicellular communities known as biofilms (Costerton et al., 1987; 1995). The transition to surface-attached (biofilm) growth is known to result in diverse changes in gene expression, which causes the attaching cells to become phenotypically and metabolically distinct from their planktonic ...
... sessile multicellular communities known as biofilms (Costerton et al., 1987; 1995). The transition to surface-attached (biofilm) growth is known to result in diverse changes in gene expression, which causes the attaching cells to become phenotypically and metabolically distinct from their planktonic ...
Functional Characterization of the 180
... perfect overlap with the NHE-terminal 16 amino acids of a cyanogen bromide fragment of RRp as indicated in Fig. 1. Northern analysis of M D C K cell poly(A) + R N A using this clone as a probe revealed a single m R N A species of 5.56.0 kb in length (Fig. 2), sufficient to encode a 180-kD protein, t ...
... perfect overlap with the NHE-terminal 16 amino acids of a cyanogen bromide fragment of RRp as indicated in Fig. 1. Northern analysis of M D C K cell poly(A) + R N A using this clone as a probe revealed a single m R N A species of 5.56.0 kb in length (Fig. 2), sufficient to encode a 180-kD protein, t ...
GENERATION OF K581A MUTATION AND PRODUCTION OF RECOMBINANT JAK2 PROTEIN
... tyrosine protein kinases (PTKs) In addition to these two classes, a smaller group called dual-specific proteins kinases are able to phosphorylate serine and threonine residues of their substrates(Adams J. A. 2001) ...
... tyrosine protein kinases (PTKs) In addition to these two classes, a smaller group called dual-specific proteins kinases are able to phosphorylate serine and threonine residues of their substrates(Adams J. A. 2001) ...
The protein quality of raw leaf, seed and root of Moringa oleifera
... of M.oleifera. Leucine is responsible for regulating blood sugar concentrations, growth and repairs of muscles/tissues, hormone production, wound healing and energy production. Its deficiency causes dizziness, headaches, fatigue, depression, confusion, irritability and hypoglycemia in infants [24] R ...
... of M.oleifera. Leucine is responsible for regulating blood sugar concentrations, growth and repairs of muscles/tissues, hormone production, wound healing and energy production. Its deficiency causes dizziness, headaches, fatigue, depression, confusion, irritability and hypoglycemia in infants [24] R ...