Ypr140wp, `the yeast tafazzin`, displays a mitochondrial
... Lyso-PC [lysophosphatidylcholine (1-acylglycerophosphorylcholine] acyltransferases (EC 2.3.1.23) catalyse the acylation of lyso-PC molecules to form PC and are involved in several important physiological processes. For example, in animal cells, the acylation of lyso-PC molecules appears to be an imp ...
... Lyso-PC [lysophosphatidylcholine (1-acylglycerophosphorylcholine] acyltransferases (EC 2.3.1.23) catalyse the acylation of lyso-PC molecules to form PC and are involved in several important physiological processes. For example, in animal cells, the acylation of lyso-PC molecules appears to be an imp ...
the unfolded protein response in yeast and mammals Chris
... of the ER-resident chaperones KAR2, PDI1 and FKB2 [12], and is necessary and sufficient for transcriptional upregulation of a given gene by the UPR [13,14]. Like IRE1, HAC1 is required for stimulation of target gene transcription in response to ER stress [4,11,15]; as production of Hac1p (e.g., from ...
... of the ER-resident chaperones KAR2, PDI1 and FKB2 [12], and is necessary and sufficient for transcriptional upregulation of a given gene by the UPR [13,14]. Like IRE1, HAC1 is required for stimulation of target gene transcription in response to ER stress [4,11,15]; as production of Hac1p (e.g., from ...
Engineering of factors determining alpha-amylase and
... CGTases which have a more bulky aromatic residue (Tyr or Phe) at this position, which is highly conserved. Characterization of the F196G mutant CGTase of T. thermosulfurigenes EM1 revealed that, for unknown reasons, apart from the F196G mutation, domain E as well as a part of domain D had become del ...
... CGTases which have a more bulky aromatic residue (Tyr or Phe) at this position, which is highly conserved. Characterization of the F196G mutant CGTase of T. thermosulfurigenes EM1 revealed that, for unknown reasons, apart from the F196G mutation, domain E as well as a part of domain D had become del ...
Aspects of growth factor signal transduction in the cell cytoplasm
... of sem-5 in which an SH2 serine residue required for phosphotyrosine binding was substituted by asparagine gave a partial vulvaless phenotype. Both SH2 domains of sem-5 are required for normal function. The growth factor receptor-binding protein GRB2 containing one SH2 and two SH3 domains has been i ...
... of sem-5 in which an SH2 serine residue required for phosphotyrosine binding was substituted by asparagine gave a partial vulvaless phenotype. Both SH2 domains of sem-5 are required for normal function. The growth factor receptor-binding protein GRB2 containing one SH2 and two SH3 domains has been i ...
The RAGNYA fold: a novel fold with multiple topological variants
... permutations. Insertions of other domains into the fold are seen in versions found in Tyw3p and the ribosomal protein L1 (Figure 3A). While the insertion is in an equivalent position in both of these versions, the inserted domains themselves are unrelated, implying that they occurred independently. ...
... permutations. Insertions of other domains into the fold are seen in versions found in Tyw3p and the ribosomal protein L1 (Figure 3A). While the insertion is in an equivalent position in both of these versions, the inserted domains themselves are unrelated, implying that they occurred independently. ...
Production of functional protein hydrolysates from Egyptian
... digestibility The enzyme hydrolysis treatments resulted in a remarkable increase in the protein content (displayed as %, calculated from the amount in g/100 g extracted seed flour), while germination resulted in only a slight increase (Table 2). For both soybean and lupin seed flours it was found th ...
... digestibility The enzyme hydrolysis treatments resulted in a remarkable increase in the protein content (displayed as %, calculated from the amount in g/100 g extracted seed flour), while germination resulted in only a slight increase (Table 2). For both soybean and lupin seed flours it was found th ...
6 systems biology of cell organization
... how the parts of cells are made, and how they maintain cellular organization. To appreciate the dynamic form and function of living cells, we need to take a broader, more integrative look at their molecular components. 1. The proteome—the entire collection of proteins that a cell makes—is largely re ...
... how the parts of cells are made, and how they maintain cellular organization. To appreciate the dynamic form and function of living cells, we need to take a broader, more integrative look at their molecular components. 1. The proteome—the entire collection of proteins that a cell makes—is largely re ...
Amyloid deposits - Lindquist Lab
... cellular pathology. On the other hand compounds that antagonize the toxicity of mutant huntingtin by reducing its aggregation have been identified.38 On the surface, this appears to conflict with the notion that promoting inclusions may be beneficial, but both, solubilization and inclusion body form ...
... cellular pathology. On the other hand compounds that antagonize the toxicity of mutant huntingtin by reducing its aggregation have been identified.38 On the surface, this appears to conflict with the notion that promoting inclusions may be beneficial, but both, solubilization and inclusion body form ...
N x C (N-2)
... microbodies. The one property which all microbodies had was that they contained large amounts of catalase, much more than any other other organellee in the cell. Christiam de Duve had first isolated lysosomes which, unlike peroxisomes, are derived from the RER/Golgi system. Analysis of the lysosome- ...
... microbodies. The one property which all microbodies had was that they contained large amounts of catalase, much more than any other other organellee in the cell. Christiam de Duve had first isolated lysosomes which, unlike peroxisomes, are derived from the RER/Golgi system. Analysis of the lysosome- ...
Atg18 function in autophagy is regulated by specific sites within its b
... into atg18D mutant cells to perform the GFP-Atg8 processing assay. This is a well-established method to monitor bulk autophagy in yeast by measuring the accumulation of free GFP in the vacuole over time (Cheong and Klionsky, 2008). When this assay was performed in wild type cells, a band of 25 kDa c ...
... into atg18D mutant cells to perform the GFP-Atg8 processing assay. This is a well-established method to monitor bulk autophagy in yeast by measuring the accumulation of free GFP in the vacuole over time (Cheong and Klionsky, 2008). When this assay was performed in wild type cells, a band of 25 kDa c ...
Auxin-binding proteins without KDEL sequence in the moss Funaria
... Estelle 2002), releasing the inhibitory effect on auxin response factors (ARFs), transcription factors that in turn regulate auxin responsive gene expression (Quint and Gray 2006; Benjamins and Scheres 2008). It is, however, evident that not all auxin responses are regulated via this nuclear recepto ...
... Estelle 2002), releasing the inhibitory effect on auxin response factors (ARFs), transcription factors that in turn regulate auxin responsive gene expression (Quint and Gray 2006; Benjamins and Scheres 2008). It is, however, evident that not all auxin responses are regulated via this nuclear recepto ...
Identification of a novel testis‐specific gene and its potential roles in
... other cell types. Most of these genes also exhibit stagespecific expression during spermatogenesis, which could be considered as spermatogenic cell type-specific since the occurrence of different spermatogenic cell types is also stage-specific during spermatogenesis. Therefore, the spermatogenic cel ...
... other cell types. Most of these genes also exhibit stagespecific expression during spermatogenesis, which could be considered as spermatogenic cell type-specific since the occurrence of different spermatogenic cell types is also stage-specific during spermatogenesis. Therefore, the spermatogenic cel ...
Short transmembrane domains with high
... way to the plasma membrane. Recent bioinformatic analyses on a large number of single-spanning membrane proteins support the hypothesis that specific features of the transmembrane domain (TMD) are relevant to the sorting of these proteins to particular organelles. Here we experimentally test this hy ...
... way to the plasma membrane. Recent bioinformatic analyses on a large number of single-spanning membrane proteins support the hypothesis that specific features of the transmembrane domain (TMD) are relevant to the sorting of these proteins to particular organelles. Here we experimentally test this hy ...
Light-dependent Dl Protein Synthesis and Translocation Is
... shown in Fig. 2 were subjected to nondenaturing gel electrophoresis, which preserves the intactness of chlorophyll-protein complexes including RCII (equivalent to CCII+a in Ref. 17) (Fig. 3). The polypeptide compositions of the protein complexes at the end of pulse (P) and chase (C) are shown in Fig ...
... shown in Fig. 2 were subjected to nondenaturing gel electrophoresis, which preserves the intactness of chlorophyll-protein complexes including RCII (equivalent to CCII+a in Ref. 17) (Fig. 3). The polypeptide compositions of the protein complexes at the end of pulse (P) and chase (C) are shown in Fig ...
Use of mRNA- and protein-destabilizing elements to develop a
... Optimizing the mRNA- and protein-fusion domains Having developed an improved assay for quantifying the effect of mRNA- or protein-DE, we used this system to determine the optimal fusion domains and degradation signals for use in transcriptional reporter assays. The nonamer UUAUUUAUU has been shown t ...
... Optimizing the mRNA- and protein-fusion domains Having developed an improved assay for quantifying the effect of mRNA- or protein-DE, we used this system to determine the optimal fusion domains and degradation signals for use in transcriptional reporter assays. The nonamer UUAUUUAUU has been shown t ...
Gram-Negative Bacteria: “Inner” vs. “Cytoplasmic” or “Plasma
... Mycobacteria and other Corynebacterineae are Gram-positives which exhibit an outer membrane containing mycolic acid and other complex lipids [10], but see also ref 2 for additional models of outer cell envelopes in prokaryotes). In contrast to those of Gram-negatives, their outer membranes appear to ...
... Mycobacteria and other Corynebacterineae are Gram-positives which exhibit an outer membrane containing mycolic acid and other complex lipids [10], but see also ref 2 for additional models of outer cell envelopes in prokaryotes). In contrast to those of Gram-negatives, their outer membranes appear to ...
Transporters of nucleotide sugars, nucleotide
... mutant transporter proteins followed by reconstitution into liposomes should allow determination of structural motifs required for membrane insertion, nucleotide recognition, and specific sugar translocation. Studies of the yeast GDPase, which plays a pivotal role in the antiport mechanism for GDP-m ...
... mutant transporter proteins followed by reconstitution into liposomes should allow determination of structural motifs required for membrane insertion, nucleotide recognition, and specific sugar translocation. Studies of the yeast GDPase, which plays a pivotal role in the antiport mechanism for GDP-m ...
1 - Columbia University
... genetics allows investigators to eliminate specific proteins by ‘knocking out’ genes; to increase the concentrations of particular proteins by increasing the number of copies of the corresponding genes or by using a more active promoter on such genes; or to alter the function of a protein by introdu ...
... genetics allows investigators to eliminate specific proteins by ‘knocking out’ genes; to increase the concentrations of particular proteins by increasing the number of copies of the corresponding genes or by using a more active promoter on such genes; or to alter the function of a protein by introdu ...
Slide 1
... AS it is proved in past that machine learning techniques are elegant in classifying the biological data. Hau and sun applied the SVM for classification of prokaryotic and eukaryotic proteins and shown that it is better than statistical methods as well as other machine learning techniques such as AN ...
... AS it is proved in past that machine learning techniques are elegant in classifying the biological data. Hau and sun applied the SVM for classification of prokaryotic and eukaryotic proteins and shown that it is better than statistical methods as well as other machine learning techniques such as AN ...