Make:targeting signals
... properties of Aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J. Cell. Comp. Physiol., 59: 223-239. [470 nm] ...
... properties of Aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J. Cell. Comp. Physiol., 59: 223-239. [470 nm] ...
The Caenorhabditis elegans mRNA 5`
... single protein of approximately 70 kDa. Identification of an extended C. elegans capping enzyme open reading frame – Previously, we (3) and others (28) found that the predicted gene C03D6 (GenBank accession no. Z75525) from the C. elegans genome sequencing project had significant similarity to the y ...
... single protein of approximately 70 kDa. Identification of an extended C. elegans capping enzyme open reading frame – Previously, we (3) and others (28) found that the predicted gene C03D6 (GenBank accession no. Z75525) from the C. elegans genome sequencing project had significant similarity to the y ...
Identification of A Novel, N-ethylmaleimide
... endosome--*TGN transport factor-1. Kinetic experiments showed that ETF-1 acts at a very early stage in vesicular transport, which may reflect a role for this factor in the formation of nascent transport vesicles. GTP hydrolysis appears to be required throughout the transport reaction. The ability of ...
... endosome--*TGN transport factor-1. Kinetic experiments showed that ETF-1 acts at a very early stage in vesicular transport, which may reflect a role for this factor in the formation of nascent transport vesicles. GTP hydrolysis appears to be required throughout the transport reaction. The ability of ...
Targeting to the T. gondii plastid
... is mediated by an N-terminal bipartite targeting sequence composed of an ER signal sequence followed by a chloroplast transit peptide-like domain (Schwartzbach et al., 1998). Like the chloroplasts of diatoms and euglenoids, the T. gondii apicoplast appears to have arisen by secondary endosymbiosis ( ...
... is mediated by an N-terminal bipartite targeting sequence composed of an ER signal sequence followed by a chloroplast transit peptide-like domain (Schwartzbach et al., 1998). Like the chloroplasts of diatoms and euglenoids, the T. gondii apicoplast appears to have arisen by secondary endosymbiosis ( ...
Identification of Antigenic Regions of Duck Hepatitis B Virus Core
... of sequence identity after sequence alignment with DHBc protein as described by Bringas (2). Distance restraints and dihedral angles were calculated on the template structure. These measures were performed for all common atoms revealed by the alignment between the DHBc and HBc proteins. The CNS 1.0 ...
... of sequence identity after sequence alignment with DHBc protein as described by Bringas (2). Distance restraints and dihedral angles were calculated on the template structure. These measures were performed for all common atoms revealed by the alignment between the DHBc and HBc proteins. The CNS 1.0 ...
+TIPs and Microtubule Regulation. The Beginning of the Plus End in
... at a capture site (Gundersen et al., 2004). In budding yeast, 1TIPs provide bridges between MT and actin cytoskeletons at specific sites and at specific times during the cell cycle (Heil-Chapdelaine et al., 1999). In motor-driven transport, the 1TIP is cargo on a kinesin that walks toward the plus e ...
... at a capture site (Gundersen et al., 2004). In budding yeast, 1TIPs provide bridges between MT and actin cytoskeletons at specific sites and at specific times during the cell cycle (Heil-Chapdelaine et al., 1999). In motor-driven transport, the 1TIP is cargo on a kinesin that walks toward the plus e ...
Hyperproduction of Exocellular Levansucrase by
... Release of cell-bound levansucrase by washing the bacteria with high ionic strength solution Joyeux et al. (1971) showed that cell-bound levansucrase in B. subtilis BS5 may be released from the cells by washing with a high ionic strength buffer. We determined for both induced strains the extent of r ...
... Release of cell-bound levansucrase by washing the bacteria with high ionic strength solution Joyeux et al. (1971) showed that cell-bound levansucrase in B. subtilis BS5 may be released from the cells by washing with a high ionic strength buffer. We determined for both induced strains the extent of r ...
Elements involved in light regulation of the parsley chs promoter: cis
... The presence of AcEchslll and Box IV, which were defined by the in viva footprinting experiments upstream of L.RU 1, motivated the construction of chs promoter-uidA fusions carrying these upstream elements in the context of various other parts of the chs promoter (Schulze-Lefert et al., 1989a). The ...
... The presence of AcEchslll and Box IV, which were defined by the in viva footprinting experiments upstream of L.RU 1, motivated the construction of chs promoter-uidA fusions carrying these upstream elements in the context of various other parts of the chs promoter (Schulze-Lefert et al., 1989a). The ...
X-ray structures of the N and C-terminal domains of a
... Mutiple packing modes of CTD dimers. The CTD dimer is involved in various intermolecular interactions in the three crystal forms studied. The presence of one dimer (CTD1 and CTD3) and four dimers in the asymmetric unit of the CTD2 crystal form (Tables 1 and 2) permits an analysis of dimerdimer inte ...
... Mutiple packing modes of CTD dimers. The CTD dimer is involved in various intermolecular interactions in the three crystal forms studied. The presence of one dimer (CTD1 and CTD3) and four dimers in the asymmetric unit of the CTD2 crystal form (Tables 1 and 2) permits an analysis of dimerdimer inte ...
Although the administration of testosterone clearly causes marked
... The effect of varying the time of testosterone pretreatment from 12 hours to 3 days is illustrated in Table II. In this experiment protein specific activity from both valine-C14 and tyrosine-C04 was doubled within 12 hours and reached a maximum of a five- to sixfold increase within 1 or 2 days after ...
... The effect of varying the time of testosterone pretreatment from 12 hours to 3 days is illustrated in Table II. In this experiment protein specific activity from both valine-C14 and tyrosine-C04 was doubled within 12 hours and reached a maximum of a five- to sixfold increase within 1 or 2 days after ...
Circadian Rhythm of Intestinal SucraseActivity in Rats
... were then incubated at 37°C for 60 min, followed by centrifugation for 60 min at 105,000 g at 4°C. (Addition of the protease inhibitor phenylmethyl sulfonyl fluoride at a concentration of 0.5 mg/ml after 60 min of incubation with papain and its inclusion during immunoprecipitation did not affect the ...
... were then incubated at 37°C for 60 min, followed by centrifugation for 60 min at 105,000 g at 4°C. (Addition of the protease inhibitor phenylmethyl sulfonyl fluoride at a concentration of 0.5 mg/ml after 60 min of incubation with papain and its inclusion during immunoprecipitation did not affect the ...
Structural basis of ubiquitylation Andrew P VanDemark and
... for efficient polyubiquitylation of a model substrate [15], U-boxes have been shown to bind E2 enzymes and to autoubiquitylate when tested using the standard assay for RING E3 activity (reviewed in [16]). Like RING domains, U-boxes are typically embedded within longer proteins that also contain doma ...
... for efficient polyubiquitylation of a model substrate [15], U-boxes have been shown to bind E2 enzymes and to autoubiquitylate when tested using the standard assay for RING E3 activity (reviewed in [16]). Like RING domains, U-boxes are typically embedded within longer proteins that also contain doma ...
selection of antigens for antibody-based proteomics
... is to recognize foreign particles and to target those particles for elimination [Travers et al. 2007]. The natural antibody response can be exploited in proteomics by immunizing an animal with a protein of interest (or parts of that protein), followed by harvest of the resulting antibodies, for use ...
... is to recognize foreign particles and to target those particles for elimination [Travers et al. 2007]. The natural antibody response can be exploited in proteomics by immunizing an animal with a protein of interest (or parts of that protein), followed by harvest of the resulting antibodies, for use ...
Controlling subcellular delivery to optimize
... the cell membrane, which has negatively charged sulfated proteoglycans on the cell surface [20] . Conjugation with cell-penetrating peptides can also overcome the barrier of the cell membrane [22,23] . In the late 1980s and early 1990s many groups ( [24–26] and others) found that a protein transduct ...
... the cell membrane, which has negatively charged sulfated proteoglycans on the cell surface [20] . Conjugation with cell-penetrating peptides can also overcome the barrier of the cell membrane [22,23] . In the late 1980s and early 1990s many groups ( [24–26] and others) found that a protein transduct ...
LIPID-TRANSFER PROTEINS IN PLANTS
... from crystallographic studies by Shin et al (97); (B) diagram of wheat LTP established from NMR studies by Gincel et al (36). The eight conserved cysteines form four disulfide bridges at the same positions suggested by previous chemical studies of castor bean LTP (107). In the absence of a bound lig ...
... from crystallographic studies by Shin et al (97); (B) diagram of wheat LTP established from NMR studies by Gincel et al (36). The eight conserved cysteines form four disulfide bridges at the same positions suggested by previous chemical studies of castor bean LTP (107). In the absence of a bound lig ...
Structural biology and drug discovery for protein–protein
... molecule binding that disrupts such interactions, will provide a path to the development of novel therapeutics which drug the ‘undruggable’. Understanding the nature and classes of PPIs for drug discovery Protein–protein interactions may be characterised as obligate, in which the subunits are not ob ...
... molecule binding that disrupts such interactions, will provide a path to the development of novel therapeutics which drug the ‘undruggable’. Understanding the nature and classes of PPIs for drug discovery Protein–protein interactions may be characterised as obligate, in which the subunits are not ob ...
Vol. 147, No. 3, 1987 September 30, 1987 BIOCHEMICAL AND
... Ca~-/calmodulin dependent protein kinase exhibits, as side activity, PI kinase activity ; the specific lipid kinase activity a~sociated with the phosphorylase kinase preparations is about I nmol min-" mg-" [20,21S. In the experiments presented in this paper phosphorylase kinase has been used as exog ...
... Ca~-/calmodulin dependent protein kinase exhibits, as side activity, PI kinase activity ; the specific lipid kinase activity a~sociated with the phosphorylase kinase preparations is about I nmol min-" mg-" [20,21S. In the experiments presented in this paper phosphorylase kinase has been used as exog ...
University of Groningen The Plasma Membrane of
... membrane for PC or PE, respectively, or to deplete the membrane of PS (4, 5). Several membrane-associated processes are affected by changes in the lipid composition; e.g., the apparent affinity constants for transport of various amino acids are increased in cells enriched with either PC or PE (198, ...
... membrane for PC or PE, respectively, or to deplete the membrane of PS (4, 5). Several membrane-associated processes are affected by changes in the lipid composition; e.g., the apparent affinity constants for transport of various amino acids are increased in cells enriched with either PC or PE (198, ...
SpeeDB: fast structural protein searches
... their study, they identified that almost half of the sulphur atoms from cysteine (S–H) and more than half the sulphur atoms from cysteine (S–S) and methionine are within 6Å from the aromatic ring centroid and predominantly approached the dþ edge of the aromatic rings as compared to the d–p-cloud. T ...
... their study, they identified that almost half of the sulphur atoms from cysteine (S–H) and more than half the sulphur atoms from cysteine (S–S) and methionine are within 6Å from the aromatic ring centroid and predominantly approached the dþ edge of the aromatic rings as compared to the d–p-cloud. T ...
Onset of lactation in the bovine mammary gland:
... Abstract The mammary gland undergoes dramatic functional and metabolic changes during the transition from late pregnancy to lactation. To better understand the molecular events underlying these changes, we analyzed expression profiles of approximately 23,000 gene transcripts in bovine mammary tissue ...
... Abstract The mammary gland undergoes dramatic functional and metabolic changes during the transition from late pregnancy to lactation. To better understand the molecular events underlying these changes, we analyzed expression profiles of approximately 23,000 gene transcripts in bovine mammary tissue ...
View PDF - Genetics
... recognize the termination codons, but in certain instances, near-cognate tRNAs over compete and lead to readthrough. tRNA decoding of a stop codon occurs more frequently when the stop codon is surrounded by a context that modifies the competition for stop codon recognition between release factor and ...
... recognize the termination codons, but in certain instances, near-cognate tRNAs over compete and lead to readthrough. tRNA decoding of a stop codon occurs more frequently when the stop codon is surrounded by a context that modifies the competition for stop codon recognition between release factor and ...
Sporopollenin biosynthetic enzymes interact and constitute a
... Anthers of angiosperm flowers produce the male microsporocytes which undergo meiosis and give rise to tetrads of four haploid microspores. During anther development, the microspores develop into pollen grains relying on both sporophytic and gametophytic gene functions (Blackmore et al., 2007; Owen ...
... Anthers of angiosperm flowers produce the male microsporocytes which undergo meiosis and give rise to tetrads of four haploid microspores. During anther development, the microspores develop into pollen grains relying on both sporophytic and gametophytic gene functions (Blackmore et al., 2007; Owen ...
NMR analysis of protein interactions
... (with 3% of the genes in the human genome) is the CCHH-type zinc-finger (ZF) motif. Although this motif is mainly associated with DNA regulatory proteins such as transcription factors, it has been found to bind RNA as well. The X-ray structure of the complex of a triple ZF domain of TFIIIA with a tr ...
... (with 3% of the genes in the human genome) is the CCHH-type zinc-finger (ZF) motif. Although this motif is mainly associated with DNA regulatory proteins such as transcription factors, it has been found to bind RNA as well. The X-ray structure of the complex of a triple ZF domain of TFIIIA with a tr ...
Import of ADP/ATP Carrier into Mitochondria: Two Receptors Act in
... possess equivalents of MOM19 and MOM72 or whether N. crassa has developed mechanisms for import of precursor proteins that are different from the mechanisms in yeast. For this report, we analyzed the import pathway of the most abundant mitochondrial protein, the inner membrane protein AAC, in yeast ...
... possess equivalents of MOM19 and MOM72 or whether N. crassa has developed mechanisms for import of precursor proteins that are different from the mechanisms in yeast. For this report, we analyzed the import pathway of the most abundant mitochondrial protein, the inner membrane protein AAC, in yeast ...