Import of ADP/ATP Carrier into Mitochondria: Two Receptors Act in
... possess equivalents of MOM19 and MOM72 or whether N. crassa has developed mechanisms for import of precursor proteins that are different from the mechanisms in yeast. For this report, we analyzed the import pathway of the most abundant mitochondrial protein, the inner membrane protein AAC, in yeast ...
... possess equivalents of MOM19 and MOM72 or whether N. crassa has developed mechanisms for import of precursor proteins that are different from the mechanisms in yeast. For this report, we analyzed the import pathway of the most abundant mitochondrial protein, the inner membrane protein AAC, in yeast ...
Low-temperature anaerobic digestion is associated with differential
... 2 from Methanomicrobiales. Amongst these, 16 proteins were involved in methanogenesis (from CO2 and acetate) including 7 proteins displaying differential expression in a temperaturedependent manner (Table 1). Remarkably, a protein involved in methanogenesis from CO2 , (tetrahydromethanopterin Smethy ...
... 2 from Methanomicrobiales. Amongst these, 16 proteins were involved in methanogenesis (from CO2 and acetate) including 7 proteins displaying differential expression in a temperaturedependent manner (Table 1). Remarkably, a protein involved in methanogenesis from CO2 , (tetrahydromethanopterin Smethy ...
Biochemistry Lecture 4 9/6/01
... • Aa’s are weak acids, so can construct titration curves for each – REMEMBER: Add OH-, measuring change in pH as titrate w/ OH-. Plot OH- added on x axis vs. pH on y axis ...
... • Aa’s are weak acids, so can construct titration curves for each – REMEMBER: Add OH-, measuring change in pH as titrate w/ OH-. Plot OH- added on x axis vs. pH on y axis ...
Stability, catalytic versatility and evolution of the
... different chemical intermediates in the various active sites. An interesting member of the enolase superfamily is an enzyme from Amycolaptosis sp., which acts both as N-acyl amino acid racemase and as o-succinylbenzoate synthase [24•]. These two enzymatic activities are considerably different with r ...
... different chemical intermediates in the various active sites. An interesting member of the enolase superfamily is an enzyme from Amycolaptosis sp., which acts both as N-acyl amino acid racemase and as o-succinylbenzoate synthase [24•]. These two enzymatic activities are considerably different with r ...
An LL-Diaminopimelate Aminotransferase
... acceptor, a linear formation of 440-nm absorbing material was observed over a period of 90 min (Fig. 2A). The rate of the reaction was directly proportional to the amount of protein extract added (Fig. 2B). No activity was observed if extract was omitted or when either LL-DAP or 2-OG were absent fro ...
... acceptor, a linear formation of 440-nm absorbing material was observed over a period of 90 min (Fig. 2A). The rate of the reaction was directly proportional to the amount of protein extract added (Fig. 2B). No activity was observed if extract was omitted or when either LL-DAP or 2-OG were absent fro ...
Unit 8A
... oxidative phosphorylation as a source of adenosine triphosphate. Although skeletal muscle can tolerate this shift in metabolism, consequences for brain function could be devastating due to its heavy reliance on mitochondrial oxidative phosphorylation to generate the energy needed for cellular proces ...
... oxidative phosphorylation as a source of adenosine triphosphate. Although skeletal muscle can tolerate this shift in metabolism, consequences for brain function could be devastating due to its heavy reliance on mitochondrial oxidative phosphorylation to generate the energy needed for cellular proces ...
Yeast as a screening tool
... cleavage of the amyloid precursor protein (APP) by the bsecretase (BACE) and the g-secretase. Because BACE knockout mice have significantly reduced Ab levels in the brain, but develop normally and show no apparent phenotype, BACE is considered an ideal therapeutic target for treating AD. We have est ...
... cleavage of the amyloid precursor protein (APP) by the bsecretase (BACE) and the g-secretase. Because BACE knockout mice have significantly reduced Ab levels in the brain, but develop normally and show no apparent phenotype, BACE is considered an ideal therapeutic target for treating AD. We have est ...
Selenium incorporation using recombinant techniques
... host as it is relatively inexpensive and simple to culture, often yielding 2 mg of purified unlabelled protein per litre of cell culture (Martzen et al., 1999; Gelperin et al., 2005). There are also examples of the production of SeMet-labelled proteins in both Pichia pastoris and Saccharomyces cerev ...
... host as it is relatively inexpensive and simple to culture, often yielding 2 mg of purified unlabelled protein per litre of cell culture (Martzen et al., 1999; Gelperin et al., 2005). There are also examples of the production of SeMet-labelled proteins in both Pichia pastoris and Saccharomyces cerev ...
The Golgi Stack Reassembles during Telophase before Arrival of
... After a 60-min chase two more doublets appeared which collapsed to a single band after digestion with neuraminidase (data not shown) confirming their identity as HLA-A bearing either one or two sialic acid residues. The structure of HLA has been well-documented (reviewed by Bjorkman and Parham, 1990 ...
... After a 60-min chase two more doublets appeared which collapsed to a single band after digestion with neuraminidase (data not shown) confirming their identity as HLA-A bearing either one or two sialic acid residues. The structure of HLA has been well-documented (reviewed by Bjorkman and Parham, 1990 ...
Chp 19
... Section 19.2: Protein Synthesis §80S complex - initiation complex binds the 60S subunit §Hydrolysis of GTP bound to eIF2 §eIF5-acts as a guanine nucleotide activating protein §Initiation factors are released from the ribosome ...
... Section 19.2: Protein Synthesis §80S complex - initiation complex binds the 60S subunit §Hydrolysis of GTP bound to eIF2 §eIF5-acts as a guanine nucleotide activating protein §Initiation factors are released from the ribosome ...
Lipids affect the function of membrane proteins
... “These lipids are critical for mediating interactions in the membrane,” Robinson says. In collaboration with David Drew
of Stockholm University, Robinson’s team used mass spectrometry to study the role of lipids
in three analogous proteins called anti ...
... “These lipids are critical for mediating interactions in the membrane,” Robinson says. In collaboration with David Drew
Aphelenchoides besseyi
... are unable to synthesize fatty acids and retinol by themselves. To sustain their life activities, plant parasitic nematodes have to obtain these metabolites from their hosts and the environment through the lipid binding proteins (LBPs) [4,5]. Nematodes have been found to produce a series of unusual ...
... are unable to synthesize fatty acids and retinol by themselves. To sustain their life activities, plant parasitic nematodes have to obtain these metabolites from their hosts and the environment through the lipid binding proteins (LBPs) [4,5]. Nematodes have been found to produce a series of unusual ...
Role of N-linked oligosaccharide chains in the processing and
... have a deleterious effect on the integrity of the H protein. With the knowledge that four sites on the H glycoprotein are used for N-linked glycosylation, further mutants in which two, three or all four sites were mutated were generated as described in Methods and are schematically illustrated in Fi ...
... have a deleterious effect on the integrity of the H protein. With the knowledge that four sites on the H glycoprotein are used for N-linked glycosylation, further mutants in which two, three or all four sites were mutated were generated as described in Methods and are schematically illustrated in Fi ...
Amino Acid Sequence Homology of Coat Proteins as a Basis for
... (Matthews, 1982; Francki et al., 1985) accounting for about one-quarter of all viruses known to infect plant species around the world. Potyviruses exist in numerous strains or pathotypes which differ mainly in biological properties, such as host range or pathogenicity (Hollings & Brunt, 1981 b). Def ...
... (Matthews, 1982; Francki et al., 1985) accounting for about one-quarter of all viruses known to infect plant species around the world. Potyviruses exist in numerous strains or pathotypes which differ mainly in biological properties, such as host range or pathogenicity (Hollings & Brunt, 1981 b). Def ...
Heterogeneity of Genome and Proteome Content in Bacteria
... Chlamydia species, whereas CG is high or overrepresented in a- and b-proteobacterial genomes (except CAUCR) and mostly normal in g-proteobacteria. CG is low in 7/11 archaea with HALSP high. The dinucleotide GC is high in many g- and e-proteobacterial genomes and also in the low C+G Gram-positive LIS ...
... Chlamydia species, whereas CG is high or overrepresented in a- and b-proteobacterial genomes (except CAUCR) and mostly normal in g-proteobacteria. CG is low in 7/11 archaea with HALSP high. The dinucleotide GC is high in many g- and e-proteobacterial genomes and also in the low C+G Gram-positive LIS ...
Functional analysis of a type-2C protein phosphatase (AtPP2C52) in
... or/and degradation (Lammers and Lavi, 2007). In Arabidopsis, seventy-six PP2C genes were identified (Kerk et al., 2002). These genes were clustered into several groups, based on their sequence similarity (Schweighofer et al., 2004; Xue et al., 2008). Group A PP2C genes are annotated as negative regu ...
... or/and degradation (Lammers and Lavi, 2007). In Arabidopsis, seventy-six PP2C genes were identified (Kerk et al., 2002). These genes were clustered into several groups, based on their sequence similarity (Schweighofer et al., 2004; Xue et al., 2008). Group A PP2C genes are annotated as negative regu ...
Effects of Enzyme Concentration, Temperature, pH
... and 9) and heated at 95°C for 15 min. The hydrolysis was carried out by mixing 50 mL of protein solutions with 50 mL enzyme solutions of different concentrations to give the final enzyme concentration of 1.0%, 1.5% and 2.0% (w/w substrate). The mixture was then incubated at a speed of 200 rpm in a s ...
... and 9) and heated at 95°C for 15 min. The hydrolysis was carried out by mixing 50 mL of protein solutions with 50 mL enzyme solutions of different concentrations to give the final enzyme concentration of 1.0%, 1.5% and 2.0% (w/w substrate). The mixture was then incubated at a speed of 200 rpm in a s ...
Hereditary neutropenia - University of Washington
... disorders that cause cutaneous and retinal hypopigmentation and bleeding, resulting from abnormal platelet densegranules [31,32]. Seven human and 14 mouse genes have been cloned [33], and all appear to be involved in lysosomal transport. HPS2 is the only type featuring neutropenia. Mammalian cells c ...
... disorders that cause cutaneous and retinal hypopigmentation and bleeding, resulting from abnormal platelet densegranules [31,32]. Seven human and 14 mouse genes have been cloned [33], and all appear to be involved in lysosomal transport. HPS2 is the only type featuring neutropenia. Mammalian cells c ...
Folding quality control in the export of proteins by the
... Robinson and Bolhuis, Nature, May 2001 ...
... Robinson and Bolhuis, Nature, May 2001 ...
Lab Protein and Amino Acids
... amino acids contain one carboxyl group but more than one amino or amino-like group. Aqueous solutions of basic amino acids have a basic pH (>7). Acidic amino acids contain one amino group but more than one carboxylic acid group. Aqueous solutions of acidic amino acids have an acidic pH (<7). Since ...
... amino acids contain one carboxyl group but more than one amino or amino-like group. Aqueous solutions of basic amino acids have a basic pH (>7). Acidic amino acids contain one amino group but more than one carboxylic acid group. Aqueous solutions of acidic amino acids have an acidic pH (<7). Since ...
Impact of glucose uptake rate on recombinant protein production in
... in lower growth rates and lower accumulation of acetic acid in comparison to the wild type. By using the mutants in batch cultivations, the formation of acetic acid to levels detrimental to cell growth could be avoided, and ten times higher cell density was reached. Thus, the use of the mutant strai ...
... in lower growth rates and lower accumulation of acetic acid in comparison to the wild type. By using the mutants in batch cultivations, the formation of acetic acid to levels detrimental to cell growth could be avoided, and ten times higher cell density was reached. Thus, the use of the mutant strai ...
Conservation of inner nuclear membrane targeting sequences in
... NLS region that we tested was Pom121 (Doucet et al., 2010; Yavuz et al., 2010; Funakoshi et al., 2011). Pom121 is a singlepass membrane protein with a small lume nal domain at the N-terminus and a large C-terminal extralumenal domain. The NLS region of Pom121, containing four or five boxes of basic ...
... NLS region that we tested was Pom121 (Doucet et al., 2010; Yavuz et al., 2010; Funakoshi et al., 2011). Pom121 is a singlepass membrane protein with a small lume nal domain at the N-terminus and a large C-terminal extralumenal domain. The NLS region of Pom121, containing four or five boxes of basic ...
Thermal unfolding of proteins at high pH range studied by UV
... Received 17 May 1996; revised 18 October 1996; accepted 23 October 1996 ...
... Received 17 May 1996; revised 18 October 1996; accepted 23 October 1996 ...
Histidine protein kinases: key signal transducers outside the animal
... HPKs also have phosphatase activity which dephosphorylates the response regulator and opposes kinase function [3,13] (for details see the Mechanism section); phosphatase activity is mediated by the dimerization domain in these HPKs. The catalytic domain of HPKs has clear sequence and structural homo ...
... HPKs also have phosphatase activity which dephosphorylates the response regulator and opposes kinase function [3,13] (for details see the Mechanism section); phosphatase activity is mediated by the dimerization domain in these HPKs. The catalytic domain of HPKs has clear sequence and structural homo ...