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Identification and temporal expression of putative circadian clock
Identification and temporal expression of putative circadian clock

... the expression of period (per) and timeless (tim). The translation of per and tim throughout the evening and early night results in the cytoplasmic accumulation of their cognate proteins that subsequently form stable heterodimers and translocate to the nucleus where they interfere with the action of ...
Tubular reabsorption
Tubular reabsorption

... Sodium: on basolateral sides, Na-K pump, creates negatives inside  increases Nainflux. B. Secondary active : Co-transport, countertransport . Na -Glcose , Na - a.a , Na - H+ C. Pinocytosis: Proteins . ...
Protein Engineering in the Development of
Protein Engineering in the Development of

... of hybrid hydrogels in response to binding events (15, 48–50). Nature is rich with diversity: There are many protein domains and peptide motifs with interesting ligand binding properties, with structures that undergo conformation changes, and that have complex functions yet to be used in responsive ...
Membrane Structure and Function Chapter 7 Biology
Membrane Structure and Function Chapter 7 Biology

... • Cells recognize each other by binding to surface molecules, often carbohydrates, on the plasma membrane • Membrane carbohydrates may be covalently bonded to lipids (forming glycolipids) or more commonly to proteins (forming glycoproteins) • Carbohydrates on the external side of the plasma membrane ...
Sequence identity and homology
Sequence identity and homology

... a local alignment algorithm decide where to stop? By lengthening the alignment only insofar as it increases the score. For example, one could increase the score by +2 for every identical amino acid, while assigning a penalty of -1 for every mismatch or gap. Such penalties would prevent the alignment ...
Classification and substrate head-group specificity of membrane
Classification and substrate head-group specificity of membrane

Pore-Exposed Tyrosine Residues of P
Pore-Exposed Tyrosine Residues of P

Analysis of protein carbonylation
Analysis of protein carbonylation

... for assigning the value to a calibrator/standard. In the field of protein carbonylation there are no reference ...
Domain structure of a human general
Domain structure of a human general

... shown). These smaller N-terminal polypeptides were retained on a Nickel column even in the presence of 6M guanidine-HCl or 8M urea indicating the presence of a histidine repeat at their Nterminus. When the cell ly sates were prepared in the presence of various protease inhibitors (PMSF, antipain, be ...
PBI 6 Features on Teacher`s Map 2-08.qxp
PBI 6 Features on Teacher`s Map 2-08.qxp

... rule is a consequence of the mechanism that cells use to begin protein synthesis; a special Met-tRNA initiates assembly of the two subunits of the ribosome at the beginning of each mRNA. (In β-globin protein, and many other proteins, this N-terminal Met is cleaved from the protein soon after it is s ...
Planta
Planta

... change during development and determine to a great extent the TSS (total soluble solids) of the fruit. The TSS, together with the total fruit acidity are key fruit quality determinants and determine whether the fruit can be marketed. Total sugar content in the fruit is determined by the relative inX ...
HHMI meeting, FOLDING
HHMI meeting, FOLDING

... This was a “chemical logic”. However, although protein folding intermediates (like MG) were found for many proteins, the main question as to how the protein chain can find its native structure among zillions of alternatives remained unanswered. A progress in the understanding was achieved when studi ...
Marshall Nirenberg - Nobel Lecture
Marshall Nirenberg - Nobel Lecture

... reported that DNAase inhibited in vitro amino acid incorporation into protein. I had also observed this phenomenon and was greatly interested in it because the results strongly suggested that the cell-free synthesis of protein was dependent, ultimately, upon DNA templates. Heinrich Matthaei then joi ...
[PDF]
[PDF]

... genetic saturation (see Experimental Procedures). These frequencies suggest that the screen was effectively saturating for loci at which a simple loss-of-function mutation interacts reliably with sev:dFmr1. dlgl is located close to a telomere, and it could be that the large number of dlgl alleles re ...
Protein structure
Protein structure

... The book has a homepage at http://www.bioinfbook.org including hyperlinks to the book chapters. ...
Structural and functional features of Drosophila chorion proteins s36
Structural and functional features of Drosophila chorion proteins s36

... and more than 14 minor chorion proteins can be resolved by two-dimensional gel electrophoresis; subsets of these proteins are expressed in a temporally regulated mode during the 5 h of choriogenesis 5 7. The sequences of all major chorion proteins have been determined: the lower molecular weight s15 ...
Ammonium Dodecyl Sulfate as an Alternative to Sodium Dodecyl
Ammonium Dodecyl Sulfate as an Alternative to Sodium Dodecyl

... cannot be predicted in the protein MALDI spectrum, the degree of mass shift is unknown. As a consequence, the protein mass cannot be accurately defined. Effects of ADS, TDS, and HDS on Resolution. SDS is commonly used to extract hydrophobic or membrane proteins from cells.5,6 Most biopolymer samples ...
Cold-Shock Response in Microorganisms
Cold-Shock Response in Microorganisms

... 20 °C or less) and psychrotolerant (growth at low temperature but with a maximum growth temperature above 20 °C) microorganisms have evolved cellular processes for growth where low temperature is the norm. Therefore, while there is a low temperature limit that even cold adapted microorganisms are un ...
New concepts of endoplasmic reticulum function in the heart
New concepts of endoplasmic reticulum function in the heart

... a peri-nuclear network that is contiguous with the nuclear envelop (Figs. 1C and D), while other evidence suggests that protein synthesis may also take place in the SR [11–14]. This latter concept is supported by findings that at least part of the SR is physically contiguous with the peri-nuclear ER ...
Plant and Soil
Plant and Soil

... Plasma membrane H+ -ATPases of plants and fungi are composed of a single catalytic polypeptide with an approximate molecular weight of 100 kDa that is phosphorylated and dephosphorylated during its catalytic cycle (Briskin and Hanson, 1992; Serrano, 1989). This property has led to the classification ...
Arabidopsis Genes Encoding Components of the
Arabidopsis Genes Encoding Components of the

Tricarboxylic Acid Cycle (TCA), Krebs Cycle
Tricarboxylic Acid Cycle (TCA), Krebs Cycle

... The inner mitochondrial membrane is impermeable to the most charged and hydrophilic substances. However it contains numerous transport proteins that permit the passage of specific molecules. 1- ATP-ADP transport, see oxid-phospho, Transporter for ADP & Pi from cytosol into mitochondria by specialize ...
mitoNEET as a novel drug target for mitochondrial dysfunction
mitoNEET as a novel drug target for mitochondrial dysfunction

... mitophagy [38]. The bioenergetics of mitochondrial function refers to the mitochondrial function and dysfunction, which essentially refers to the ability of mitochondria to respond to cellular energy demands and need for ATP synthesis [39]. mitoNEET null ( / ) mice did not appear to have an apparent ...
Powerpoint - Wishart Research Group
Powerpoint - Wishart Research Group

... Gene Annotation* • Annotation – to identify and describe all the physico-chemical, functional and structural properties of a gene including its DNA sequence, protein sequence, sequence corrections, name(s), position, function(s), abundance, location, mass, pI, absorptivity, solubility, active sites ...
PDF Format - Kinexus Bioinformatics Corporation
PDF Format - Kinexus Bioinformatics Corporation

... substrates in lysates from cultured cells. Clients should contact Kinexus if they are interested in having rabbit polyclonal antibodies developed to recognize novel proteins identified from our mass spectrometry analyses. ...
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Magnesium transporter

This page links directly from the magnesium in biological systems page.Magnesium transporters are proteins that transport magnesium across the cell membrane. All forms of life require magnesium, yet the molecular mechanisms of Mg2+ uptake from the environment and the distribution of this vital element within the organism are only slowly being elucidated.In bacteria, Mg2+ is probably mainly supplied by the CorA protein and, where the CorA protein is absent, by the MgtE protein. In yeast the initial uptake is via the Alr1p and Alr2p proteins, but at this stage the only internal Mg2+ distributing protein identified is Mrs2p. Within the protozoa only one Mg2+ transporter (XntAp) has been identified. In metazoa, Mrs2p and MgtE homologues have been identified, along with two novel Mg2+ transport systems TRPM6/TRPM7 and PCLN-1. Finally, in plants, a family of Mrs2p homologues has been identified along with another novel protein, AtMHX.
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