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Note: This insert is lot specific. For information on other lots, please contact our Technical Services Department or your local sales office. Product: Synonyms: Anti-CIN85, C-Terminal, Human (Rabbit) Cbl-Interacting Protein of 85 kDa Cat. No.: 231006 Lot No.: Size: Storage: Usage: B50401 100 µl −20°C ELISA (0.1 mg/ml); immunoblotting (1:500); immunofluorescence (1:100); immunoprecipitation 1-2 g Product Specifications: Source: General Description: Immunogen: Formulation: Preservative: Specificity: Special Instructions: Potency/Assay Conditions: Germany Tel 0800 6931 000 Rabbit Recognizes CIN85, a protein involved in the degradation of several tyrosine kinase receptors including epidermal growth factor (EGF), hepatocyte growth factor (HGF), platelet derived growth factor (PDGF), and c-Kit receptors. Also known as Ruk (regulator of ubiquitous kinase) and SETA (SH3 domain-containing gene expressed in tumorigenic astrocytes), CIN85 is an ubiquitously expressed adaptor protein with three SH3 domains. It interacts with several proteins including Cbl, Cbl-b, Grb2, Crk, and p130Cas. CIN85 is involved in endosomal sorting and degradation of receptor tyrosine kinases. Following ligand binding, receptor tyrosine kinases become phosphorylated, bind to, and phosphorylate Cbl or Cbl-b. These proteins polyubiquitinate the phosphorylated receptor and then recruit and monubiquitinate CIN85. CIN85 is constitutively associated with endophilins which are involved in clathrin-coated pit formation and subsequent endocytosis. This leads to the degradation of the activated receptor. It has recently been reported that malignant cells seem to escape the rapid mechanisms of down regulation despite their association with Cbl/Cbl-b and CIN85. Immunogen used was a synthetic peptide (KIRLRLQMEVNDIKKALQSK) corresponding to amino acid residues 646 - 665 of CIN85. Affinity purified polyclonal antibody supplied at 1 mg/ml in PBS, pH 7.4. None Detects the ~85 kDa CIN85 protein in human, mouse, porcine, and rat. Will also detect the mono-ubiquinated form of CIN85. Avoid freeze/thaw cycles. Variables associated with assay conditions will dictate the proper working dilution. USA & Canada Tel (800) 628-8470 United Kingdom Tel 0115 9430 840 E-mail address for technical inquiries: [email protected] Find our current product data sheets on the web: http://www.calbiochem.com Revised: 21-Jan-03 CN: References: Haglund, K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 12191. Petrelli, A., et al. 2002. Nature 416, 187. Souberyan, P., et al. 2002. Nature 416, 183. Szymkiewicz, I., et al. 2002. J. Biol. Chem., in press. For research use only, not for diagnostic use.