Repair of larynx, trachea, and other fibrocartilaginous tissues
... mercial softWare package produced by DNAstar, Inc. The teachings by both sources are incorporated by reference herein. An initial alignment can be re?ned by comparison to a multi-sequence alignment of a family of related proteins. Once the alignment betWeen the candidate sequence and the seven-cyste ...
... mercial softWare package produced by DNAstar, Inc. The teachings by both sources are incorporated by reference herein. An initial alignment can be re?ned by comparison to a multi-sequence alignment of a family of related proteins. Once the alignment betWeen the candidate sequence and the seven-cyste ...
procite - UWI St. Augustine
... specific to the consensus sequence Asn-Xaa-Ser/Thr. It must be noted that the presence of the consensus tripeptide is not sufficient to conclude that an asparagine residue is glycosylated, due to the fact that the folding of the protein plays an important role in the regulation of N-glycosylation [2 ...
... specific to the consensus sequence Asn-Xaa-Ser/Thr. It must be noted that the presence of the consensus tripeptide is not sufficient to conclude that an asparagine residue is glycosylated, due to the fact that the folding of the protein plays an important role in the regulation of N-glycosylation [2 ...
Identification and Cloning of a Cryptococcal Deacetylase That
... Therefore, thè Identification of cryptococcal antigens capable of producing T-cell-mediated responses, sudi as delayed-type hypersensitivity (DTH) reactions, may be useful in thè development of immune-based strategies to contro! cryptococcosis. In order to characterize DTH-producing antigens, cultur ...
... Therefore, thè Identification of cryptococcal antigens capable of producing T-cell-mediated responses, sudi as delayed-type hypersensitivity (DTH) reactions, may be useful in thè development of immune-based strategies to contro! cryptococcosis. In order to characterize DTH-producing antigens, cultur ...
ASD v2.0: updated content and novel features
... pathway tools in response to community demands. Up-to-date allosteric pathways were manually curated in the updated version. In addition, both the front-end and the back-end of ASD have been redesigned and enhanced to allow more efficient access. Taken together, these updates are useful for facilita ...
... pathway tools in response to community demands. Up-to-date allosteric pathways were manually curated in the updated version. In addition, both the front-end and the back-end of ASD have been redesigned and enhanced to allow more efficient access. Taken together, these updates are useful for facilita ...
The Bacterial Toxin RelE Displays Codon
... At a RelE concentration four times less than the concentration of ribosomes that were competent in dipeptide formation (Zavialov et al., 2001; Experimental Procedures), peptide synthesis from the mRNA with UAG as stop codon was completely blocked by the toxin. With twenty times less RelE than active ...
... At a RelE concentration four times less than the concentration of ribosomes that were competent in dipeptide formation (Zavialov et al., 2001; Experimental Procedures), peptide synthesis from the mRNA with UAG as stop codon was completely blocked by the toxin. With twenty times less RelE than active ...
Eram_SeyedMohammad - UWSpace
... native molecular weights of 260 kDa. Although the POR of T. maritima was characterized previously, its PDC activity was not reported. Since the T. hypogea genome sequence is not available, the primary structure of the genes encoding each of the POR four subunits were determined using inverse PCR and ...
... native molecular weights of 260 kDa. Although the POR of T. maritima was characterized previously, its PDC activity was not reported. Since the T. hypogea genome sequence is not available, the primary structure of the genes encoding each of the POR four subunits were determined using inverse PCR and ...
Regulation of Phenylalanine and Tyrosine
... L-tyrosine converted the substrate saturation curve to a simple Michaelis-Menten hypxbola (Fig. 4). In P. aeruginosa P A 0 1 the bifunctional enzyme chorismate mutase-prephenate dehydratase was inhibited by L-phenylalanine (Patel et al., 1977). However, inhibition of chorismate mutase was lost durin ...
... L-tyrosine converted the substrate saturation curve to a simple Michaelis-Menten hypxbola (Fig. 4). In P. aeruginosa P A 0 1 the bifunctional enzyme chorismate mutase-prephenate dehydratase was inhibited by L-phenylalanine (Patel et al., 1977). However, inhibition of chorismate mutase was lost durin ...
Characterization of an Immuno-dominant Variable Surface
... More recently several other probes, based either on DNA sequences (27-29), or the detection of specific antigens (30), have been suggested as diagnostic reagents . However, in most cases, axenic cultivation (20) and cloning (31) of amoebae directly from fresh stool samples before assay with any of t ...
... More recently several other probes, based either on DNA sequences (27-29), or the detection of specific antigens (30), have been suggested as diagnostic reagents . However, in most cases, axenic cultivation (20) and cloning (31) of amoebae directly from fresh stool samples before assay with any of t ...
NGRLW_SPODS_2.2 - National Genetics Reference Laboratories
... primers also have filler bases[s] between the GS sequence and the tag: these are included to prevent the formation of a highly stable hairpin structures. Primers with the US1 tag are designated GS1 and primers with the US2 tag are designated GS2. The final component of the system is a set of univers ...
... primers also have filler bases[s] between the GS sequence and the tag: these are included to prevent the formation of a highly stable hairpin structures. Primers with the US1 tag are designated GS1 and primers with the US2 tag are designated GS2. The final component of the system is a set of univers ...
Technical Brief
... “…the most careful examination failed to show that the dietary pattern of the children who developed kwashiorkor or marasmus was qualitatively different from those of other children in the community.” This simple sentence, published in a narrative book chapter, not in a formal scientific journal, wi ...
... “…the most careful examination failed to show that the dietary pattern of the children who developed kwashiorkor or marasmus was qualitatively different from those of other children in the community.” This simple sentence, published in a narrative book chapter, not in a formal scientific journal, wi ...
CHAPTER 1 INTRODUCTION
... Soya beans were one of the first pulses cultivated by man (Cronje , 1997). The soya bean (Glycine max (L.) (Merrill, family Leguminosae) undoubtedly originated in the Orient , probably China . As early as the sixteenth century, it was exported from Eastern Asia to Europe. Soya beans were first intro ...
... Soya beans were one of the first pulses cultivated by man (Cronje , 1997). The soya bean (Glycine max (L.) (Merrill, family Leguminosae) undoubtedly originated in the Orient , probably China . As early as the sixteenth century, it was exported from Eastern Asia to Europe. Soya beans were first intro ...
Molecular and biochemical characterization of cytosolic
... ADP-glucose (ADPG) can be synthesized in the cytosol and taken up directly by the amyloplast as well as being synthesized within the organelle. The possession of a cytosolic AGPase could bypass the need for the G1P produced by the plastidial PGM reaction, since ADPG synthesis could be directly linke ...
... ADP-glucose (ADPG) can be synthesized in the cytosol and taken up directly by the amyloplast as well as being synthesized within the organelle. The possession of a cytosolic AGPase could bypass the need for the G1P produced by the plastidial PGM reaction, since ADPG synthesis could be directly linke ...
6.
... length of constitutively spliced exons is 129 bases [16].) Thus, non-CAS exons are significantly longer than CAS exons. Which exons are functional? We detected a conserved mouse exon for 25% of the human cassette exons in our set. These CAS exons most probably have functional importance. In principl ...
... length of constitutively spliced exons is 129 bases [16].) Thus, non-CAS exons are significantly longer than CAS exons. Which exons are functional? We detected a conserved mouse exon for 25% of the human cassette exons in our set. These CAS exons most probably have functional importance. In principl ...
Functional characterization of rare FOXP2 variants in
... variants examined (p.N597H, p.P416T, p.Q390Vfs*7), this is the first report of any functional characterization. The remaining four variants have been studied previously to varying extents and are included here for comparison with the uncharacterized variants. All of the variants were additionally ch ...
... variants examined (p.N597H, p.P416T, p.Q390Vfs*7), this is the first report of any functional characterization. The remaining four variants have been studied previously to varying extents and are included here for comparison with the uncharacterized variants. All of the variants were additionally ch ...
papain, a plant enzyme of biological importance
... The protein is stabilized by three disulfide bridges in which the molecule is folded along these bridges creating a strong interaction among the side chains which contributes to the stability of the enzyme (Edwin and Jagannadham, 2000; Tsuge et al., 1999). Its three-dimensional structure consists of ...
... The protein is stabilized by three disulfide bridges in which the molecule is folded along these bridges creating a strong interaction among the side chains which contributes to the stability of the enzyme (Edwin and Jagannadham, 2000; Tsuge et al., 1999). Its three-dimensional structure consists of ...
please refer to anzfa`s guide to applications and proposals for a
... submitted an Application seeking approval for food derived from dual herbicide-tolerant soybean line DP-356043-5 (also referred to as soybean 356043) under Standard 1.5.2 – Food produced using Gene Technology, in the Australia New Zealand Food Standards Code (the Code). Soybean 356043 has been genet ...
... submitted an Application seeking approval for food derived from dual herbicide-tolerant soybean line DP-356043-5 (also referred to as soybean 356043) under Standard 1.5.2 – Food produced using Gene Technology, in the Australia New Zealand Food Standards Code (the Code). Soybean 356043 has been genet ...
Advances in Chemical Protein Modification
... relied upon reactions at nucleophilic amino acids, particularly cysteine (Cys) or lysine (Lys) residues (Scheme 1).13 Cysteine easily undergoes disulfide exchange to form mixed disulfides14 and also alkylation with suitable electrophiles that include α-halocarbonyls (e.g., iodoacetamide)14 and Michael ...
... relied upon reactions at nucleophilic amino acids, particularly cysteine (Cys) or lysine (Lys) residues (Scheme 1).13 Cysteine easily undergoes disulfide exchange to form mixed disulfides14 and also alkylation with suitable electrophiles that include α-halocarbonyls (e.g., iodoacetamide)14 and Michael ...
Fast evolutionary rates associated with functional loss in class I
... selected nodes from the ML tree topology in Fig. 1. Class I glucose transporters (node 2) and Platyhelminthes-specific cluster of glucose transporters (node 10) (Panel a). Divergence times for selected branching points were calculated in the 100 most parsimonious trees using the ML method based on t ...
... selected nodes from the ML tree topology in Fig. 1. Class I glucose transporters (node 2) and Platyhelminthes-specific cluster of glucose transporters (node 10) (Panel a). Divergence times for selected branching points were calculated in the 100 most parsimonious trees using the ML method based on t ...
Article The Evolution of Momordica Cyclic Peptides
... acyclic counterparts is given in figure 3B. A new set of primers for conserved sequences within the endoplasmic reticulum (ER) signal and the acyclic peptide domain was designed because the first primer set could not amplify TIPTOP genes from the remaining Momordica genomic DNA. PCR with this new se ...
... acyclic counterparts is given in figure 3B. A new set of primers for conserved sequences within the endoplasmic reticulum (ER) signal and the acyclic peptide domain was designed because the first primer set could not amplify TIPTOP genes from the remaining Momordica genomic DNA. PCR with this new se ...
Purification and characterization of pyruvate decarboxylase from
... during the fermentation of glucose, but 100 mM-ethanol caused only a slight reduction (22%) in pyruvate decarboxylase activity. Phosphate increased the cooperativity and decreased the apparent affinity for pyruvate of pyruvate decarboxylase from Sacch. cerevisiae (Boiteux & Hess, 1970), but 100 mM-p ...
... during the fermentation of glucose, but 100 mM-ethanol caused only a slight reduction (22%) in pyruvate decarboxylase activity. Phosphate increased the cooperativity and decreased the apparent affinity for pyruvate of pyruvate decarboxylase from Sacch. cerevisiae (Boiteux & Hess, 1970), but 100 mM-p ...
Flux analysis in central carbon metabolism in plants
... experiments that employ U-13C (uniformly 13C-labeled) substrates. It was shown that the enumeration of feasible bondomers in a metabolic network reduced the number of bondomer balances 3-fold. This resulted in a simplified system that offers the advantages of faster computation, and easier flux iden ...
... experiments that employ U-13C (uniformly 13C-labeled) substrates. It was shown that the enumeration of feasible bondomers in a metabolic network reduced the number of bondomer balances 3-fold. This resulted in a simplified system that offers the advantages of faster computation, and easier flux iden ...
Bacterial ribosome requires multiple L12 dimers for efficient initiation
... (L12 NTD)6 complex from T. maritima (1). It has been proposed (13) and later evidenced with FRET (18) that on the ribosome L12 dimer can exist with one hinge compact and the other extended. Although thought to be important for translation factor recruitment, physiological relevance of such conformat ...
... (L12 NTD)6 complex from T. maritima (1). It has been proposed (13) and later evidenced with FRET (18) that on the ribosome L12 dimer can exist with one hinge compact and the other extended. Although thought to be important for translation factor recruitment, physiological relevance of such conformat ...
Localization and nucleotide specificity of Blastocystis succinyl‐CoA
... in the absence of a classic mitochondrial electron transport chain, it is likely to be one of the main ATP producing enzymes in this parasite. Here we present the genes encoding both alpha and beta SCS subunits, their phylogenetic analysis, the cellular localization of SCS and a biochemical characte ...
... in the absence of a classic mitochondrial electron transport chain, it is likely to be one of the main ATP producing enzymes in this parasite. Here we present the genes encoding both alpha and beta SCS subunits, their phylogenetic analysis, the cellular localization of SCS and a biochemical characte ...
Genome-wide Investigation of Cellular Functions for tRNA Nucleus
... In eukaryotic cells tRNAs are transcribed in the nucleus and exported to the cytoplasm for their essential role in protein synthesis. This export event was thought to be unidirectional. Surprisingly, several lines of evidence showed that mature cytoplasmic tRNAs shuttle between nucleus and cytoplasm ...
... In eukaryotic cells tRNAs are transcribed in the nucleus and exported to the cytoplasm for their essential role in protein synthesis. This export event was thought to be unidirectional. Surprisingly, several lines of evidence showed that mature cytoplasmic tRNAs shuttle between nucleus and cytoplasm ...