Solutions to 7.014 Problem Set 1
... a) Describe the conditions of the atmosphere on prebiotic earth and how these conditions differ from what is found today. Prebiotic earth had an atmosphere that lacked oxygen, but was rich in H2, N2, CO2, H2O, and sulfurous gases. In the absence of O2 and O3, the UV light penetrated in far greater s ...
... a) Describe the conditions of the atmosphere on prebiotic earth and how these conditions differ from what is found today. Prebiotic earth had an atmosphere that lacked oxygen, but was rich in H2, N2, CO2, H2O, and sulfurous gases. In the absence of O2 and O3, the UV light penetrated in far greater s ...
Repair of larynx, trachea, and other fibrocartilaginous tissues
... activity. The unglycosylated protein, Which also has osteo genic activity, has an apparent molecular Weight of about 27kD. When reduced, the 27kD protein gives rise to tWo unglycosylated polypeptides, each having a molecular Weight of about 14kD to about 16kD. Typically, naturally occurring osteogen ...
... activity. The unglycosylated protein, Which also has osteo genic activity, has an apparent molecular Weight of about 27kD. When reduced, the 27kD protein gives rise to tWo unglycosylated polypeptides, each having a molecular Weight of about 14kD to about 16kD. Typically, naturally occurring osteogen ...
Mechanism of Posttranslational Regulation of Phenol
... DEAE sepharose fast flow and the HiTrap desalting column were obtained from Amersham Pharmacia Biotech Asia Pacific (Hong Kong). Hydroxyapatite ultrogel was purchase from Biosepra (Fremont, CA). All other chemicals were obtained commercially at the highest purity possible. Enzyme Assay. PST activity ...
... DEAE sepharose fast flow and the HiTrap desalting column were obtained from Amersham Pharmacia Biotech Asia Pacific (Hong Kong). Hydroxyapatite ultrogel was purchase from Biosepra (Fremont, CA). All other chemicals were obtained commercially at the highest purity possible. Enzyme Assay. PST activity ...
A STUDY OF THE ROLES OF SELECTED ARGININE AND
... exists in plasma as a 280 kDa tetramer [18]. CPN was shown to cleave C-terminal arginine or lysine residues of kinins [19], anaphylatoxins [20], fibrinopeptides [19], and other peptides. Unlike TAFIa, CPN does not possess any antifibrinolytic properties, although it still possesses the ability to hy ...
... exists in plasma as a 280 kDa tetramer [18]. CPN was shown to cleave C-terminal arginine or lysine residues of kinins [19], anaphylatoxins [20], fibrinopeptides [19], and other peptides. Unlike TAFIa, CPN does not possess any antifibrinolytic properties, although it still possesses the ability to hy ...
Identification and first characterization of pairing
... Furthermore, it has been shown, that females induce lipid accumulation and lipase activity in males (Haseeb et al., 1989). However, research on a factor transferred from the female to the male was neglected as it was assumed to be uncertain and less promising concerning schistosomiasis control compa ...
... Furthermore, it has been shown, that females induce lipid accumulation and lipase activity in males (Haseeb et al., 1989). However, research on a factor transferred from the female to the male was neglected as it was assumed to be uncertain and less promising concerning schistosomiasis control compa ...
Bettendorff L, Wins P. Biological functions of thiamine
... cells: thiamine protects retinal neurons against glutamate toxicity50 and promotes the survival of hippocampal neurons in high cell density culture51. Thiamine requires specific transporters to enter cells52. As the rate of transport by these transporters is relatively slow, membrane transport is a ...
... cells: thiamine protects retinal neurons against glutamate toxicity50 and promotes the survival of hippocampal neurons in high cell density culture51. Thiamine requires specific transporters to enter cells52. As the rate of transport by these transporters is relatively slow, membrane transport is a ...
STUDIES ON THE CHLORAMPHENICOL HALOGENASE CmlS
... The flavin-dependent halogenases are the most prevalent class of halogenase responsible for the regio- and stereoselective incorporation of halogens into natural products. These enzymes require FAD, O2, NADH, and a flavin reductase to perform catalysis. The majority of these enzymes are observed to ...
... The flavin-dependent halogenases are the most prevalent class of halogenase responsible for the regio- and stereoselective incorporation of halogens into natural products. These enzymes require FAD, O2, NADH, and a flavin reductase to perform catalysis. The majority of these enzymes are observed to ...
Methods and strategies of peptide ligation
... in the reacting pairs of NT-amine and CT-carboxylic acid for an orthogonal ligation to occur. There are essentially two configurations. For the NT-amine (or CT-COOH), the functional group can be placed either at the side chain or at the ␣-amine (or as an ester). A common combination for an NT-amine- ...
... in the reacting pairs of NT-amine and CT-carboxylic acid for an orthogonal ligation to occur. There are essentially two configurations. For the NT-amine (or CT-COOH), the functional group can be placed either at the side chain or at the ␣-amine (or as an ester). A common combination for an NT-amine- ...
Allelic Amino Acid Substitutions Affect the
... for over 90% of the PLAP phenotypes (2). However, more than 20 rare allozymes have been described, often only in heterozygous combinations because of their low allelic frequency (3). The two most common PLAP phenotypes, S (slow) and F (fast), differ only in an Arg#{176} to p2o9 substitution (4), but ...
... for over 90% of the PLAP phenotypes (2). However, more than 20 rare allozymes have been described, often only in heterozygous combinations because of their low allelic frequency (3). The two most common PLAP phenotypes, S (slow) and F (fast), differ only in an Arg#{176} to p2o9 substitution (4), but ...
DMD #11502 1 GENETIC POLYMORPHISM OF ALDEHYDE
... This article has not been copyedited and formatted. The final version may differ from this version. ...
... This article has not been copyedited and formatted. The final version may differ from this version. ...
An investigation of protective formulations containing
... they can cause skin irritation, dermatitis. The biggest difference between an adult skin and a baby’s skin lies primarily in the anatomical differences in the surface of the skin. The development of the ...
... they can cause skin irritation, dermatitis. The biggest difference between an adult skin and a baby’s skin lies primarily in the anatomical differences in the surface of the skin. The development of the ...
Investigations of non-enzymatic glycation with new bioanalytical
... Desalting of glycated peptides eluted from tips with sorbitol For the desalting of glycated peptides eluted from boronate affinity tips, three different solid phase extraction (SPE) sorbents were compared. Here 5 mg from fullerene-derivatized silica particles made from a silica with a pore diameter ...
... Desalting of glycated peptides eluted from tips with sorbitol For the desalting of glycated peptides eluted from boronate affinity tips, three different solid phase extraction (SPE) sorbents were compared. Here 5 mg from fullerene-derivatized silica particles made from a silica with a pore diameter ...
Evidence for Several Cysteine Transport Mechanisms in the
... The mitochondrial fraction incubated with radiolabeled [35S]cysteine for 20 min at 25 C was layered on top of a modified 1.4 ml mini-Percoll step gradient (Millar et al. 2001, Howell et al. 2006). After centrifugation, the mitochondrial fraction appeared as a yellow-brownish band between the 25% an ...
... The mitochondrial fraction incubated with radiolabeled [35S]cysteine for 20 min at 25 C was layered on top of a modified 1.4 ml mini-Percoll step gradient (Millar et al. 2001, Howell et al. 2006). After centrifugation, the mitochondrial fraction appeared as a yellow-brownish band between the 25% an ...
Glycerol transport and phosphoenolpyruvate
... Tris\HCl buffer, pH 7n4. Proteins were separated by PAGE performed either under denaturing (1 % SDS) or non-denaturing conditions. Gels were dried and exposed to x-ray film (Biomax MR, Kodak) for experiments carried out with [$#P]PEP or stained with Coomassie brilliant blue R for experiments with no ...
... Tris\HCl buffer, pH 7n4. Proteins were separated by PAGE performed either under denaturing (1 % SDS) or non-denaturing conditions. Gels were dried and exposed to x-ray film (Biomax MR, Kodak) for experiments carried out with [$#P]PEP or stained with Coomassie brilliant blue R for experiments with no ...
View/Open
... first limiting amino acid. As a result, milk production can sometimes be increased when dairy cows are fed supplemental ruminally protected lysine and methionine, or when DDGS is blended with other protein supplements that contain more lysine. However, in most situations, milk production is as high, ...
... first limiting amino acid. As a result, milk production can sometimes be increased when dairy cows are fed supplemental ruminally protected lysine and methionine, or when DDGS is blended with other protein supplements that contain more lysine. However, in most situations, milk production is as high, ...
ESTUDIO DE LOS MECANISMOS DE INHIBICIÓN DE LA ACTIVIDAD CARNITINA PALMITOILTRANSFERASA 1
... analysis of the presence of residues shared by all the malonylCoA-regulated enzymes of the carnitine-choline acyltransferase family versus the malonyl-CoA nonregulated members of the same family was performed using the algorithm SequenceSpace (23, 24). This method uses a vectorial representation of ...
... analysis of the presence of residues shared by all the malonylCoA-regulated enzymes of the carnitine-choline acyltransferase family versus the malonyl-CoA nonregulated members of the same family was performed using the algorithm SequenceSpace (23, 24). This method uses a vectorial representation of ...
Regulation of mitochondrial calcium in plants versus animals
... intermolecular disulphide bond and closes the channel at low extramitochondrial Ca2+ concentrations (Patron et al., 2014; Petrungaro et al., 2015). The stability of MICU2 depends on MICU1 (Plovanich et al., 2013; Patron et al., 2014), and loss of MICU2 in MICU1-silenced cells complicates assignment ...
... intermolecular disulphide bond and closes the channel at low extramitochondrial Ca2+ concentrations (Patron et al., 2014; Petrungaro et al., 2015). The stability of MICU2 depends on MICU1 (Plovanich et al., 2013; Patron et al., 2014), and loss of MICU2 in MICU1-silenced cells complicates assignment ...
REGULATION OF CYTOCHROME P450 BY
... transmembrane helix at the N-terminus of the protein, which also serves as a targeting sequence for the signal recognition particle dependent cotranslational incorporation of a nascent P450 into the ER membrane (Bar-Nun et al., 1980; Sakaguchi et al., 1984). Insertion continues until a halt-transfer ...
... transmembrane helix at the N-terminus of the protein, which also serves as a targeting sequence for the signal recognition particle dependent cotranslational incorporation of a nascent P450 into the ER membrane (Bar-Nun et al., 1980; Sakaguchi et al., 1984). Insertion continues until a halt-transfer ...
Characterization of an Immuno-dominant Variable Surface
... La Raza-IMSS Hospitals, Mexico City. Diagnosis of hepatic abscess in patients was established by clinical symptoms, countercurrent immunoelectrophoresis, ELISA, and rectosigmoidoscopy. Human sera from donors without history of amoebiasis and negative for anti-amoebic antibodies as tested by immunobl ...
... La Raza-IMSS Hospitals, Mexico City. Diagnosis of hepatic abscess in patients was established by clinical symptoms, countercurrent immunoelectrophoresis, ELISA, and rectosigmoidoscopy. Human sera from donors without history of amoebiasis and negative for anti-amoebic antibodies as tested by immunobl ...
DMD #620 1 Identification of di (2-ethylhexyl) phthalate
... detector (Hitachi), a model AS-2000 autosampler (Hitachi), a model D-2500 integrator ...
... detector (Hitachi), a model AS-2000 autosampler (Hitachi), a model D-2500 integrator ...
evaluation of l-methionine bioavailability in nursery pigs
... Protein is an important nutrient in swine production. There have been many studies to define the amino acid (AA) requirement because a deficiency in protein supply to the diet can cause severe reduction in growth and reproduction and an excessive protein supplementation can increase feed cost and N ...
... Protein is an important nutrient in swine production. There have been many studies to define the amino acid (AA) requirement because a deficiency in protein supply to the diet can cause severe reduction in growth and reproduction and an excessive protein supplementation can increase feed cost and N ...
Physiological Strategies During Animal Diapause: Lessons from
... photoperiod or food quality/availability. Depending on the developmental stage, diapause may be hormonally regulated and occur in response to signaling factors that come before the adverse period. The phenomenon is widespread across animal phyla and critically important for surviving in extreme envi ...
... photoperiod or food quality/availability. Depending on the developmental stage, diapause may be hormonally regulated and occur in response to signaling factors that come before the adverse period. The phenomenon is widespread across animal phyla and critically important for surviving in extreme envi ...
Effects of low crude-protein diets fortified with crystalline amino acids
... Corn and soybean meal (SBM) are included in most pig and broiler diets as sources of protein. When corn and SBM are used to supply all of the protein, the diet contains amino acids in excess of the pigs' or broilers' requirements. The balance of amino acids in a given corn-SBM diet is different from ...
... Corn and soybean meal (SBM) are included in most pig and broiler diets as sources of protein. When corn and SBM are used to supply all of the protein, the diet contains amino acids in excess of the pigs' or broilers' requirements. The balance of amino acids in a given corn-SBM diet is different from ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.