Download Transcriptome and Proteome Analysis Of Arum Lily

XLII Annual Meeting of SBBq
th
st
Foz do Iguaçu, PR, Brazil, May 18 to 21 , 2013
Transcriptome and Proteome Analysis
Of Arum Lily (Zantedeschia aethiopica) Spathe Defense System
Cândido, E.S.1,2; Cardoso, M.H.S.2; Oliveira Júnior, N.G.2,3; Fernandes, G.R.1, de
Alencar, S.A.1; Porto, W.F.1,2; Nolasco, D.O.1,4; Barbosa, A.E.A.D.1,2; Lima, S.M.F.1,2;
Miranda, V.J.1,2; Magalhães, B.S.1,2; Dias, S.C.1,2 and Franco, O.L.1,2
1
Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia,
Universidade Católica de Brasília, Brasília-DF, Brazil; 2Centro de Análises
Proteômicas e Bioquímicas, Pós-Graduação em Ciências Genômicas e
Biotecnologia, Universidade Católica de Brasília, Brasília-DF, Brazil; 3Programa de
Pós-Graduação em Biologia Animal, Universidade de Brasília, Brasília-DF, Brazil.
4
Curso de Física, Universidade Católica de Brasília, Brasília – DF, Brazil.
INTRODUCTION. Z. aethiopica is an evergreen perennial plant from Araceae
botanical family worldwide known for ornamental purposes being also utilized for
African traditional medicine including as antimicrobial source. Nevertheless, as
others non-model plants, this species has no abundant molecular and physiological
data available and the RNA-Seq technology shows an enormous potential to afford
in-depth coverage and impartial portrayal of transcripts abundance, which is essential
to works without a reference genome. In this view, in order to characterize molecular
and physiologically defense aspects of spathe tissue, transcriptome and proteome
analysis were performed. MATERIAL AND METHODS: Z. aethiopica spathe tissue
was utilized as source for transcriptomics and proteomics analysis. RNA-Seq
technology was applied aiming to acquire transcripts data, which were trimmed and
de novo assembled. Functional annotation was carried out using KEGG and NR
NCBI databases. 2-DE gels were performed and protein spots were further identified
analyzed by MALDI-TOF MS/MS being sequences obtained by de novo sequencing.
Transcripts and protein sequences were compared aiming to provide insights into the
relationship between the gene expression and protein function. RESULTS AND
DISCUSSION: RNA-Seq data obtained allowed finding transcripts involved specially
in genetic information (37 %) and metabolism (34 %) processes. In this view, proteins
involved in plant hormone signal transduction, carbon fixation in photosynthetic
organisms, photosynthesis and photosynthesis-antenna pathways, which are
intrinsically associated with plant defense, were here identified. CONCLUSION:
Floral protein maps were drawn suggesting that major spathe efforts were applied to
guarantee the cell homeostasis maintenance in view of high investment on
carbohydrate, amino acids and energy metabolism as well as in genetic information,
mainly for defense mechanisms.
Keywords: Transcriptome, Proteome, RNA-Seq, Spathe
Acknowledgments: UCB, CNPq and CAPES