Download Exercise 3 key

\_~-4------------------
€hem--l56-Exerdse-3
Names ____
Due Thurs, March 20
Transaminases using PLP
NH +
~3
CH 3
+ -o2c~co2-
co2·
alanine
transaminase
o
a-ketoglutarate
a-keto acid (pyruvate)
glutamate
The transamination reaction above requires the coenzyme pyridoxal-5-phospate (PLP) to carry out the
amino group transfer from alanine to ketoglutarate to form Glutamate and pyruvate. YOl.ftext on pg
721 shows the mechanism for stage I of this reaction; the transformation of the amino acid to the keto
acid (we also did this in lecture).
A Draw the arrow pushing mechanism for stage II of this reaction which transforms a-Ketoglutarate to
glutamate. Be sure to use an arrow pushing mechanism as done on pg 721 of Voet.
B What is the fate of the glutamate product in this reaction?
~~ lc.....Jo1 h.•~"~ -t ~\-\'l +ouf...t~
c'1 e.~