Download Responding to STD/HIV Increases and Viagra Use

Jeffrey D. Klausner, MD, MPH
Professor of Medicine and Public Health
Attending Physician Ronald Reagan Medical Center
Center for World Health and Division of Infectious Diseases
David Geffen School of Medicine
Department of Epidemiology
Karin and Jonathan Fielding School of Public Health
School of Public Health
Room 71-257 CHS
February 13, 2013
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Dr. Klausner is a faculty member of the University of California Los Angeles
Dr. Klausner is a guest researcher with the US CDC Mycotics Diseases Branch
Dr. Klausner is a member of the WHO Congenital Syphilis Elimination advisory group
Dr. Klausner is medical director, AIDS Project Los Angeles Health and Wellness Center
Dr. Klausner is a board member of Isis-Inc.
In the past 36 months, Dr. Klausner has received:
 Salary from the US CDC as a federal employee (7/2009-11/2011)
 Travel support for meeting attendance from WHO, London School of Hygiene and Tropical
Medicine and Council of Scientific Industrial Research, South Africa
 Grant funding, supplies or unrestricted educational gifts for research from the NIH, CDC,
California HIV Research Program, Hologic Gen-Probe, Inc., Gilead Sciences, Inc., Cepheid,
Inc., AIDS Project Los Angeles, Public Health Foundation Enterprises, American Jewish
World Service and Qpid.me
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An estimated 1,148,200 persons in the United States live with HIV
About 50,000 new HIV infections occur in the United States each year
Each year, approximately 16-22 million persons in the United States are tested
for HIV
An estimated 38%-44% of all adults had been tested for HIV
Approximately 1 in 5 (18%, or 207,600 persons) do not know they are HIVinfected.
US CDC, 2012 http://www.cdc.gov/hiv/resources/factsheets/us.htm
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Earlier detection leads to improved treatment outcomes
HIV-infection status awareness is associated with reduced transmission
risk behavior
HIV-infected persons on treatment are less infectious
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RNA virus
Surface envelope
proteins
 Matrix proteins
 Capsule proteins
 RNA
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Detect human antibodies to surface proteins
Detect human antibodies to surface, matrix and capsule proteins
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Detect HIV antigen (P24 capsule protein)
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Detect RNA
Detect DNA, integrated within cells
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1st generation—viral lysate
2nd generation—synthetic antigen + HIV-2
3rd generation—IgM and IgG
4th generation—p24 + IgM and IgG
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Enzyme-linked immunosorbent assay (EIA)
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1st generation—viral lysate
 2nd generation—synthetic antigen and HIV-2
 3rd generation—IgM and IgG
 4th generation—p24 + IgM and IgG
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Western blot
Immunofluorescent assay
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ELISA = Enzyme-Linked Immuno Sorbent Assay.
This technique is based on the lock and key theory of antibodies.
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Antibodies and antigens work like locks and keys.
One antibody fits one antigen.
Having the antibody means the antigen is also present.
ELISA technique involves placing HIV antigens (locks) on the bottom of a microwell cup
The microwell is then filled with the serum to be tested.
If the appropriate anti-HIV antibodies are present (keys), they will stick to the antigens
(locks).
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Since antibodies are proteins too, they
themselves are also antigens.
Scientist developed an anti-HIV
antibody antibody. So this new
antibody sticks to the back of the first
antibody.
This second antibody has an enzyme is
attached to it.
When a reactive substrate is added to
the mix, the enzyme will turn the
substrate a different color (usually red).
If the serum to be tested contains antiHIV antibodies, the liquid in the
microwell will turn red.
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Includes HIV-2 antigen
Uses synthetic antigens
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• OraQuick Rapid HIV-1/2 Antibody Test
• Reveal G3 Rapid HIV-1 Antibody Test
• Uni-Gold Recombigen HIV Test
• Multispot HIV-1/HIV-2 Rapid Test
• Clearview HIV 1/2 Stat Pak
• Clearview Complete HIV 1/2
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The method of 3rd generation of HIV
ELISA test is double antigen sandwich
 Detect all kinds of antibody of HIV
 Improved sensitivity and specificity as well.
 The main laboratory-based diagnostic
ELISA test worldwide now.
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The method of 4th generation of HIV
ELISA test is that HIV antigen and p24
antigen coat the vector simultaneously
 Detect the HIV P24 and HIV antibody in
the sample at the same time.
 So, it could be used for detection for the
samples of window period which shows
positive in HIV P24 antigen tests but is not
transformed into HIV infection.
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• Identifies antibodies against eight HIV-1 encoded
proteins: p18, p24, p31, gp41, p51, p55, p65/66,
gp120/p160.
• Criteria require antibodies against any two of the
following HIV-1 proteins: p24, gp41, gp120/160.
• Specimens showing reactivity to HIV-1 protein(s), but
not fulfilling the criteria for a positive result, are reported
as Indeterminate.
• All indeterminate Western blots are further tested in
supplemental HIV-1 and HIV-2 specific assays.
• A negative Western blot has no detectable bands, i.e.
no antibodies reacting to either HIV-1 or non-HIV-1
proteins.
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HIV Immunoflourescent assay
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Used to diagnose HIV infection
Resolve indeterminate HIV-antibody results
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Used to monitor antiviral therapy and to predict disease progression in
HIV infected persons.
In conjunction with a positive DNA PCR or a reactive EIA, the RNA
quantitation may be diagnostic.
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High levels of RNA are found during acute infection and in patients who are more likely
to have disease progression.
 Inhibition of cell-free HIV, as reflected by RNA copy number, is associated with better
CD4 response and clinical response in some patient populations.
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The dynamic range for HIV RNA detection by Real-Time PCR is 30 to
1,000,000 copies/mL of plasma.
Often use in newborns and infants for early diagnosis
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The detection of cell associated Human Immunodeficiency Proviral DNA
by polymerase chain reaction (PCR) amplification is one of the most
sensitive non-serologic methods for confirming HIV infection.
This assay is recommended for confirming HIV infection in the neonate.
HIV DNA PCR may also be used as a supplemental test to determine the
significance of an indeterminate HIV Western Blot serology result.
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Culture is an extremely sensitive virologic method for documenting HIV
infection, especially in neonates whose serologies are complicated by the
presence of maternal antibody
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The assay involves sequencing of the HIV pol gene, after which
mutations in the gene can be compared to sequences known to confer
resistance to different classes of antiretroviral drugs.
The assay is most useful in patients who lose viral suppression on
antiretroviral therapy and should be performed before switches in
therapy are entertained.
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30 year old bisexual man comes into clinic
He has had 15 lifetime partners, never been HIV-tested
What test is appropriate?
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22 year old man who has sex with men, methamphetamine user
Last tested HIV-negative 6 months ago
History of syphilis
What test is appropriate?
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46 year old man diagnosed with HIV-infected 6 years ago
Has been on treatment for 3 years but has not had a check up in a year
What test is appropriate?
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17 year old girl had a rapid HIV test that was positive
She comes to clinic for testing
What test is appropriate?
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17 year old girl had a rapid HIV test that was positive
The ELISA test was indeterminate
What test is appropriate?
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47 year old man has been on treatment for years but ran out of meds 1
year ago.
About 6 months ago he restarted 2 medications he obtained from his
partner.
He has been losing weight and complains of fatigue and fevers
What test is appropriate?
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6 week baby had an HIV-infected mother
The mother receive treatment during pregnancy
What test is appropriate for the baby?
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[email protected]
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