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Name _____________________________________________________ Per. ___________ Date _________ Unit 4 Study Guide: Molecular Genetics Questions Genes (DNA) contain the instructions for making what? What is the monomer for DNA & RNA? A nucleotide is made up of three parts List the bases found in DNA & RNA Answer DNA & RNA are made up of ___________________________ bonded together! 1. 2. 3. Purines:________________ & ________________ Pyrimidines:______________ &________________ (In RNA thymine is replaced with _______________) Which bases bond together? How do we remember these base-pairing rules? ________________ bonds with _______________ ________________ bonds with_______________ ____________________=___________________ ____________________=___________________ Label the deoxyribose sugar, phosphate, base pairs, hydrogen bonds, and nucleotide The shape of DNA is a _______________________ What was Rosalind Franklin's x-ray diffraction image used to determine? Name _____________________________________________________ Per. ___________ Date _________ Draw a double bubble to show 4 similarities and 3 differences between DNA & RNA What happens during DNA replication? Why is DNA replication called "semiconservative"? List the four main enzymes involved in DNA replication (in order!) and their functions (job) What is the "Central Dogma" for Protein Synthesis? Name & describe the two main steps of protein synthesis: Enzyme Function (job) _______ --> _______ --> _______ --> ________ 1. ________________________(DNA ->mRNA) 2. ________________________(mRNA ->Protein) Define intron & exon. intron: exon: Circle the one that carries useful information for making proteins? Name _____________________________________________________ Per. ___________ Date _________ LABEL: ribosome, mRNA, tRNA, amino acid, codon, anticodon Explain the functions of mRNA & tRNA: Function of mRNA:__________________________ Function of tRNA:___________________________ Define codon and anticodon and include which type of RNA they are found on: Proteins are: Use the DNA sequence to: 1. Write the mRNA sequence: 2. Use your codon table to determine the amino acid sequence: If the mRNA sequence is AUUCGUAUGCUGAAC 1. Write the DNA sequence: 2. Use your codon table to determine the amino acid sequence: codon: anti-codon: ____________ ___________ connected by _____________ bonds! DNA: TAC GGA TCG AAT CGC mRNA:___________________________________ A.A.: ____________________________________ DNA:____________________________________ mRNA: AUU CGU AUG CUG AAC A.A.: ____________________________________ How can there be so many different proteins when there are only 20 amino acids? Do your skin cells have the same DNA as your blood cells? How is it that these cells make different proteins? Name _____________________________________________________ Per. ___________ Date _________ Define Mutation: In what two types of cells do mutations occur? 1. What are the two types of gene mutations 1. What is a point mutation? 2. Circle where the mutation occurred in the example 1. Describe the two types of frameshift mutations 2. Circle where the mutation has occurred in each of the examples 1. 2. 2. Point Mutation: Example: Insertion mutation: Example: DNA: ATT GCG Mutation: ATT TGC G Deletion mutation: Example: What is Recombinant DNA? Describe the two steps of Genetic Engineering in detail! DNA: ATT GCG Mutation: ATT ACG DNA: ATT GCG Mutation: ATT CG 1. Creating Recombinant DNA: 2. Introducing the gene into a new organism What is the job of a restriction enzyme? Genetic Engineering Application: What are the two uses of genetic engineering? Genetic engineering has been used to produce goats whose milk contains __________ (medicine) by injecting new DNA into ______________________________. 1. 2.