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College Prep Cellular Respiration Notes: H.B.3A.4 Harvesting Chemical Energy • The food you eat cannot be used by cells directly. • Cells have only one usable energy form, ATP (adenosine triphosphate). • Cellular Respiration is the complex process in which cells make ATP by breaking down organic compounds. • Any food (organic) molecule, or nutrient, including carbohydrates, fats/lipids, and proteins can be processed and broken down as a source of energy to produce ATP molecules. • nutrients + oxygen water + energy (ATP) + carbon dioxide What is the ATP produced during cellular respiration used for? • ATP can then release the energy for cellular metabolic processes: – Active transport - Protein Synthesis - Muscle Contraction What type of organism carry out cellular respiration? • Autotrophs – Use the glucose created during photosynthesis • Heterotrophs – Use organic compounds that are harvested from digestion Cellular Respiration Occurs in Two Stages • Step 1: Glycolysis – Glyco = Sugar -Lysis = To Break Apart If Oxygen is available: • Step 2: Aerobic Respiration : Aerobic = requires oxygen – The two steps of aerobic respiration are: 1. Citric acid cycle or Krebs cycle 2. Electron Transport Chain Where Does Cellular Respiration Occur? Glycolysis occurs in the cytoplasm • The Krebs cycle occurs in the mitochondria Review of Mitochondria Structure • Smooth outer Membrane • Folded inner membrane • Folds called cristae • Space inside cristae called the matrix Many Reactions in Cellular Respiration are REDOX reaction • A chemical reaction in which there is the transfer of one or more electrons from one reactant to another. • Oxidation is the loss of electrons • Reduction is the gain of electrons. • Because the electron transfer requires a donor and an acceptor, oxidation and reduction always go together. What Carries the Electrons? • NAD+ (nicotinadenine dinucleotide) acts as the energy carrier • NAD+ is a coenzyme • It’s reduced to NADH when it picks up two electrons and one hydrogen ion Glycolysis • Breaks down glucose into 2 Pyruvate molecules (aka pyruvic acid) • Anaerobic = Does not require Oxygen • Carbon Dioxide is released • Net of 2 ATP molecules are made • Occurs in a series of reactions that are catalyzed by specific enzymes Summary of Glycolysis ****THIS WILL BE ON THE TEST***** Location ATP USED ATP created Net ATP Product Cytoplasm 2 4 2 2 Pyruvates The Kreb’s Cycle: A Little History • Discovered by Hans Krebs in 1937 • He received the Nobel Prize in physiology or medicine in 1953 for his discovery • Forced to leave Germany prior to WWII because he was Jewish The Krebs Cycle • Sometimes called the Citric Acid Cycle • Requires oxygen = aerobic • Takes place in the mitochondria • Has a second step: Electron Transport Chain (ETC) Summary Krebs Cycle It takes two turns in the cycle to break down one molecule of glucose. Location Reactants Products Mitochondrial Matrix Pyruvic acid 4CO2molecules 6NADH 2FADH2 2ATP The Second Step: Electron Transport Chain (ETC) • Most of the energy storing ATP molecules are formed during this step. • Series of chemical reactions that combine hydrogen + oxygen to make water. • CO2 is released as a waste product • 34 ATP molecules produced Adding up ATP Step of Cell Respiration Net number of ATP molecules Glycolysis 2 Krebs cycle 2 Electron Transport Chain (ETC) 32 TOTAL: 36 Cellular Respiration Equation • Does not represent all of the steps that occur during the process of aerobic respiration. • C6H12O6 + 6O2 → 6CO2 + 6H2O + Energy • • • • One glucose molecule and six oxygen molecules are needed to produce six carbon dioxide molecules and six water molecules. Each of the reactants (glucose and oxygen) is used during different stages of aerobic respiration. – Glucose used during glycolysis – Oxygen used during Krebs Cycle and ETC Each of the products (carbon dioxide and water) is formed during different stages of the process The energy that is released is primarily used to produce approximately 32 to 36 of ATP per glucose molecule. What Do Cells Do When Oxygen is not available? • Cells can obtain energy through anaerobic respiration. • An = not/without oxygen • • If there is no O2 available fermentation takes place Fermentation is not as efficient as aerobic respiration – ATP is produced in minimal amounts (2 ATP) • There are two types of fermentation depending on the organism: 1. alcoholic fermentation 2. lactic acid fermentation Why am I Sore: Lactic Acid Fermentation? • Occurs in animal cells • Ex. Muscle Tissue during vigorous exercise • The pyruvic acid formed during glycolysis is broken down to lactic acid and energy is released • Glucose Pyruvic acid _Lactic acid _Energy • Occurs so that the cell can have a continual source of energy Alcoholic Fermentation • Occurs in plant cells, yeast, and some bacteria. • Glucose Pyruvic acid + _Alcohol_+ CO2 + Energy • Sugars are converted into cellular energy and produce ethanol (alcohol)and carbon dioxide as metabolic wastes. • This reaction occurs in the production of ethanol fuels, in the rising of bread dough, production of yogurt, pickles and cheese.