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Transcript
The
present
cessive
of
genetic
lethal
Neurospora.
covering
It
the
entirely
portion
analyze
the
National
is now
types
Atomic
induced
Energy
Horowitz,
N.
used
H.
of
is possible
the
and
to
deletion
of
ad-3
with
Mukai
Irreparable
occurrence
mutants
l6,
65,
in
giving
the
lower
does
not
culation
It has
always
and
on
measured
in
standard
nuclei
logicals
and
and
reparable
testing
should
prove
Pawdena,
Suzuki,
to
may
D. T.
prompts
been
the
zygote
mortality
that
the
difference
by
the
o crossover
outer
nuclei,
Cold
of
8,
Neurospora
the
would
to
Harbor
1953).
As
is
method
o class
of
functions.
have
tempero-
Spring
1027,
postulating
indispensable
NN
The
temperature-mutant
by
only
comments.
methods:
P. N.A.S.
“indispen(Curtis,
two
the
controls
of
Leupold,
& Mukoi,
genes
the
would
be
that
be tested
standard
if
this
be
genes
A simple
belong
to
this
col-
class
in
(sorbose-free)
if the
view
os
extracting
to be
irreparable
os
the
from
the
About
half
is correct.
back-
frequency
counts
scored
was
were
made
reparable
on
by
the
background
case,
lethols
mutants
here
and
ore
genetic
this
amycelial-methionineless
be found
complete.
expressed
however,
that
in ony
not
temperature-mutant
o wild-type
methionineless,
mutations
placed
would
registered
by
method,
and
all
In the
against
heterocoryon
whether
so scored
exists.
determined
omyceliol
ask
suspect
could
explanation
was
the
mutants
would
on
plausible
In
This
A system
to
Genetics
3:
but
resulted
test
for
non-random
554-604)
in
that,
bridges
and
Ridge
United
but
under
the
that
and
many
morpho-
conditions
of the
amyceliol-meth;onineless
of
the
bock-
“irreparable
---California
lethals”
InstFtute
of
Technology,
U.S.A.
therefore,
dicentric
Novitski
by
8
estimates,
In Drosophila
females,
oriented
postulated,
to
the
Neurospora
following
H orowitz
in
o row
of ascospores
(1936,
ore
Oak
for
problem
in
estimated
1950;
two
disjunction.
Beadle
in the
functions
the
mutations
reasonably
recoverable
California,
o more
auxotrophs
them
be
that
medium.
I strongly
method.
ground
Division,
Corporation
(Atwood
which
the
irreparable
carried
method
medium.
of
7)
33,
explained
and
85%
complete
One
heterocoryon
8 Mukoi
alleles
of
which
temperature-mutant
sorbose
method
to me
frequency
sorbose-complete.
on
deletions
results
discrepancy.
seemed
the
gross
and
---Biology
interest
gene
has
3,
between
Atwood
the
mutations
Genetics
discrepancy
approximately
for
in
heterocoryon
temperature
that
experiments,
the
estimate.
to account
(I)
These
re-
dikaryons
1954).
Carbide
Continued
lethal
Adv.
is o large
yield
shows
ground
and
there
which
irreparably
(Horowitz,
1951)
known,
order
of
method
Symp.
well
with
mutation-induction,
dose.
analyze
in balanced
Commission.
#2,
of
aa
and
loci
deletions.
I: 125,
Union
sable”
frequency
associated
Res.
by
Neurospora.
ture-mutant
were
terminal
of X-ray
Operated
maintain,
linked
of survival
os o function
recover,
particular
(2)
(Radiation
kinetics
Tennessee.
induce,
of
mutations
LGF
the
mutants
Ridge,
it
arm
to study
ad-3
Oak
none
right
of Atwood
being
of
that
presumptive
that
those
Laboratory,
States
shown
from
of the
with
system
hove
resulting
is noteworthy
maior
consistent
This
tests
mutations
Sandier
were
within
more
the
of the
Genetics
o tetrad,
frequently
that
recovery
four
retained
(1956,
event
found
center
&I:
196-204)
shorter
the
longer
within
non-exchange
either
in the
the
than
of
nuclei,
exchange
of
outer
of
(1951,
that
the
dyad
four
the
when
products
of
similar
to
oscus.
only
ore
orientation
ond
not
increase
offspring.
could
te
fertilized
36:
267-280)
asymmetrical
is recovered,
the
did
potroclinous
Genetics
meiosis
Sturtevont
heterozygotes
or
nuclei
Novitski
demonstrated
member.
inversion
chromotids
the
nuclei.
member
of
within
the
dyads
i.e.,
They
ond
and
are
Is included
formed
in