Download RESEARCH NOTES Creaser, E.H.

RESEARCH
Creaser,
E.H.
and
--__
from
dehydrogenase
unknown
at
histidinol
present.
con
be
extraction
The
quite
pH
product
stable
pH
glycollic
of
first
con
for
or
of
con
be
Serres,
F. J.
lethal
mutations
closely
linked
Neurospora
formation
and
sucrose
from
of
and
B.
B.
resulting
densify
some
in
Webber.
from
prevented
the
theory,
ad-3
of
heterokaryons
coverable
by
intergenic
Mukai
(Proc.
inactivate
With
this
recovery
of
“inclusive
of
induced
in o heterokaryon
any
least
39:
loci.
genetic
should
intergenic
of
ad-3
are
incubation
The
enzyme
is
cellulose.
The
for
reactivated
by
NAD,
its
thio-
o molecular
mutants
which
lock
University
expect
1953).
of
to
on
to
most
each
lethal
right
os a purple
arm
of
group
----hi+2
Ill
ad-2
IV
VI
linkage
A
recessive
genetic
in
group
from
ad-3A
adenine-
nit-2
1962,
both
adenine
locus.
should
be
In
re-
and maintain
of Atwood
lethal
ond
mutations
of
events
ore
indeterminate
that
given
simultaneously
al-2
inos
pan-2
in
Table
niacin-supplemented
I in component
cot
-
--r
793,
com-
nit-2
and
(74-OR31-16A)
ad-3B
or
deletions.
used
Component
(74-OR60-29A)
IR
V
colony
or
deficiencies
dikoryon
of
locus
with
to recover
experiments
result
os
the
only
regions
those
majority
result
adenineqequiring
B which
loci.
Component
Linkage
of
or ad-3E
lotus
chromosome
interpreted
the
The ability
from
the
mutations
component
in
hist-2
tests,
represent
simply
recover
the
their
the
the
Genet’n
in a-t
inferred
that
ore
supplemented
nearby
loci
mutations
suggest
Osterbind,
medium
the
lethal
mutations
markers
of
be
In
recessive
ad-3A
medium.
moy
recessive
in
use
into
assumed
markers
and
involving
1027,
some
These
alteration
the
the
were
that
of
involving
Serves
the
deletions
of
show
and
one
one
DEAE
histidine-3
ad-3
biochemical
ond
heterokaryon
U.S.
linked
heterokaryon
at
The
of Microbiology,
analyses
extending
large
complexes”
o series
almost
acids
discorded.
indicates
o Iso from
by
is specific
be
ultrocentrifugotion
and
Genetic
linked
alterations
from
Sci.,
experiments
medium
closely
in o balanced
Acad.
numbers
enzyme
con
of X ray-induced
experiments
supplementation
present
activates
of
these
resulting
Notl.
strain
in
of genetic
appropriate
in
The
The
used
alterations
participating
size.
Th e presence
dikaryon
mutations
such
The
sequenceThe
nucleic
is retained.
on
in
largely
mycelium
precipitate
chromatography
procedures.---Department
Recessive
deletion
balanced
data).
the
dried
prec:pitate
the
preparations
gradient
and
enzyme.
to
hvo
being
in the
this
from
enzyme
Neurosporo.
deletions
unpublished
of
and
heterocaryons
crosso.
ponent
the
these
enzyme
of
MnC12
and
of
biosynthesis
terminal
or
controls
first
histidine
powder
saturation
aged
histidine-3
the
properties
0.05M
contains
(de
and
gloss
treatment
locus
the
some
with
protein
ot
of
and
with
heat
the
enzyme
the
the
to 50%
activated
The
by
stages
the
Neurospora,
England.
and
loci
of
Normal
purified
Birmingham,
by
is Mgtt
early
which
purified
it
to 40,000,
function
precipitate
0. I%
IO,
cysteine.
36,000
Birmingham,
further
the
is treated
is added
that
in
grinding
extract
sulphate
the
is thought
functions
purification
by
The
and
be
the
mycelium
approximately
is in excess
acid
weight
wet
buffer.
65%
is to direct
studied
Ammonium
and
accounts
optimum
have
from
9. I Tris
to
It
function
We
is increased
heat
Histidinol
w.
second
proteins.
saturation
de
Drysdale.
extracted
with
unwonted
the
B.
Neurosooro
dehydrogenase.
enzyme
and
R.
NOTES
B
in-
I.