Download Report II. 2017. january

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project

Document related concepts

Protein–protein interaction wikipedia , lookup

Transcript
In vivo and in vitro characterization of discretion mutations
During the past year, we made significant progress in the characterization of the
dyskerin enzyme on three levels. First, using an allelic series that we created for this
project, we demonstrated in vivo that the enzyme is indispensible for development, and
it is necessary for protein synthesis, through its role in ribosome biogenesis. Second,
using known mutations in dyskerin, we were able to demonstrate that certain mutations
do not disrupt the composition of the complex per se, but they impair the active center,
by indirectly inducing structural changes in the complex. Finally we performed mixed
quantum mechanical/molecular mechanical calculations on the putative reaction
mechanisms put forward in the literature and we propose a novel mechanism to explain
the isomerization reaction catalyzed by the dyskerin molecule.