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(a) NOS-Ter GUS 35S-Pro 35S-Pro NOS-Ter OFP (b) Bright field * OFP filter * (c) Figure S1. Demonstration of coexpression of two reporter genes in transgenic hairy roots of soybean. (a) Schematic representation of the construct used for coexpression of an orange fluorescent protein (OFP) reporter gene and a GUS reporter gene. “35S-Pro” and “NOS-ter” represent the CaMV 35S promoter and the NOS terminator, respectively. (b) Analysis of transgenic hairy roots with the construct described in (a). OFP-positive transgenic hairy root (labeled with an asterisk) were separated from OFP-negative hairy root (labeled with a triangle) under OFP filter. (c) OFP-positive hairy roots were further demonstrated to be positive for GUS staining.