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Submission for new gene Suggested gene name – Philippine Blue Suggested gene designation – pb/pb and +/pb New Loci - yes Gene type is recessive Original cross of Blue to Wild Santa Isabel Female Male Breed by Ken Kennedy P1 P2 F1 Wild Type Santa Isabel +/+ Blue Albino Ghost S/+ - a/a - pb/pb Ghost Silver S/+ - +/pb - +/a +/pb - +/a Expected percentage 50% 50% No counts and due to the gene being recessive the assumption is that all of the F1 offspring carry a single dose. F1 mated pair from original cross Male Silver (+/a - +/pb) Female Ghost (S/+ -+/a - +/pb) Breed by Damon Cartmell F1 F1 Ghost Silver S/+ - +/pb - +/a +/pb - +/a Spawn Count F2 221 Expected Expected percentage counts Phenotype Genotype Blue Ghost Blue Silver S/+ - pb/pb pb/pb 12.50% 12.50% Silver Ghost +/pb S/+ - +/pb 25% 25% Silver Ghost +/+ S/+ Albino No survivors 12.50% 12.50% 28 28 0 55 55 0 28 28 Actual count 26 21 90 84 Combined count of heterozygous pb and wild type due to recessive nature F2 Silver (+/+ -+/pb or +/+) Heterozygous for blue gene or isn’t a carrier Blue Silver (+/+ - pb/pb) Homozygous for blue gene Silver Ghost (S/+ -+/pb or S/+ - +/+) Heterozygous for blue gene or isn’t a carrier F2 Blue Ghost (S/+ - pb/pb) F2 Silver (+/+ -+/pb or +/+) Blue Silver (+/+ - pb/pb) F2 Silver (+/+ -+/pb or +/+) Heterozygous for blue gene or isn’t a carrier Blue Silver (+/+ - pb/pb) Homozygous for blue gene Blue Silver (+/+ - pb/pb) F2 A top down view of the fish show how easily you can distinguish homozygous fish from the rest of the population A light base color vs. tan. F2 Phenotypic expression on known Genes/Alleles Blue Marble M/+ - pb/pb) Blue Clown S/Z – pb/pb) Blue Zebra Z/+ - pb/pb) Platinum Marble (Gm/g – pb/pb) Blue Smokey Sm/+ - pb/pb) Pinoy Smokey D/+ - Sm/+ -pb/pb) Pinoy Paraiba D/+ - S/S – pb/pb) Pinoy Clown D/+ - S/Z – pb/pb) Double Dark Pinoy Ghost (D/D – S/+ - pb/pb) I would like to acknowledge the hard work that Mr. Ken Kennedy did in bringing the true nature of this gene to light because without his contribution we may not be enjoying this beautiful new mutation. Based on the above information I believe it is very obvious that this is a new mutation. It is a recessive gene on it’s own Loci with no known Alleles at this time. I ask at this time for The Angelfish Society to recognize and approve the name chosen by Mr. Kenneth Kennedy. Philippine Blue (pb).
