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Georgia Performance Standards: SB3a. Explain the cycling of energy through the processes of photosynthesis and respiration. Essential Questions: -How do plants and animals get energy? -How is energy transferred from sunlight to glucose to ATP? - How is energy stored and released from food molecules? - How are the processes of photosynthesis and cellular respiration related? Hook Suppose you earned extra money by having a part-time job. At first, you might be tempted to spend all of the money, but then you decide to open a bank account. 1. What are the benefits of having a bank account? 2. What do you have to do if you need some of this money? 3. What might your body do when it has more energy than it needs to carry out its activities? 4. What does your body do when it needs energy? Warm-up: Autotroph & Heterotroph Review • Autotrophs: – Use light energy from the sun to produce their own food. (photosynthesis) • Heterotrophs: – Gain energy by consuming other source Chapter 8 Cellular Energy 8.1 How Organisms Obtain Energy Metabolism All of the chemical reactions in a cell Photosynthesis—light energy from the Sun is converted to chemical energy for use by the cell Cellular respiration—organic molecules are broken down to release energy for use by the cell 8.1: Energy & Life • ATP, made in the mitochondria of the cell, is the basic energy source of all cells. ATP = ENERGY • It stores and releases energy ATP = Adenine Ribose Adenosine Triphosphate 3 Phosphate groups Comparison of ADP and ATP to a Battery ADP ATP Energy Adenosine diphosphate (ADP) + Phosphate Partially charged battery Energy Adenosine triphosphate (ATP) Fully charged battery See video clip Chapter 8 Cellular Energy 8.1 How Organisms Obtain Energy ATP: The Unit of Cellular Energy ATP releases energy when the bond between the second and third phosphate groups is broken, forming a molecule called adenosine diphosphate (ADP) and a free phosphate group. ATP Chapter 8 Cellular Energy 8.1 How Organisms Obtain Energy Transformation of Energy Energy is the ability to do work. Thermodynamics is the study of the flow and transformation of energy in the universe. Chapter 8 Cellular Energy 8.1 How Organisms Obtain Energy Laws of Thermodynamics First law—energy can be converted from one form to another, but it cannot be created nor destroyed. Second law—energy cannot be converted without the loss of usable energy. Formative Assessment: 1.What is the ultimate source of energy for plants? 2. What is ATP and what is its role in the cell? 3. Describe cellular activities that use the energy released by ATP. 4. How do autotrophs obtain energy? How do heterotrophs obtain energy? 5. With respect to energy, how are ATP and glucose similar? Ticket Out the Door • Pg. 221 # 1-4 Warm-up: Trapping Energy • Have you ever used a solar-powered calculator? • No matter where you go, as long as you have a light source, the calculator works. You never have to put batteries in it. Warm-up: Trapping Energy 1. A solar-powered calculator uses solar cells that are found in rows along the top of the calculator. Into what kind of energy is the light energy converted so that the calculator works? 2. Recall that plants use light energy from the sun to make food. Into what kind of energy is the light energy converted by plants? 3. Most plants, no matter what size or shape they are, have some parts that are green. Which parts of a plant are usually green? 4. What does the green color have to do with the plant’s ability to convert light energy into the energy found in the food it makes? Chapter 8 Cellular Energy 8.2 Photosynthesis Overview of Photosynthesis Photosynthesis occurs in two phases. Light-dependent reactions Light-independent reactions 8.2 Photosynthesis: From solar energy to chemical energy • Photosynthesis uses the energy of sunlight to convert water and carbon dioxide into oxygen and high-energy sugars (glucose) 6CO2 + 6H2O C6H12O6 + 6O2 • Photosynthesis requires sunlight • Reactants = water (H2O) and carbon dioxide (CO2) • Products = glucose (C6H12O6) and oxygen (O2) Photosynthesis: Reactants and Products Light Energy Chloroplast CO2 + H2O Sugars + O2 Photosynthesis is a series of reactions that uses energy from the sun to convert water and carbon dioxide into sugars and oxygen. Go toPhotosynthesis takes place in a plant organelle called the chloroplast. Section: What does a chloroplast look like? The Chloroplast Dissected • Chlorophyll pigments on the thylakoid membrane in the chloroplasts of plants absorb the energy in sunlight. • That energy is transferred directly to electrons in the chlorophyll molecules, which causes photosynthesis to work. – Chlorophyll A – Chlorophyll B Classification of Electromagnetic Radiation Copyright © Houghton Mifflin Company. All 7–21 Light & Chlorophyll Pigments: • Photosynthesis Also Requires Light and Chlorophyll Pigments • Sunlight is seen as white light (a mixture of different wavelengths of light) – Wavelengths of visible light are seen as certain colors • Review Spectrum of visible Light • Plants appear green because that is the color that is reflected. • The other colors of light are being absorbed. Chlorophyll Light Absorption Absorption of Light by Chlorophyll a and Chlorophyll b Chlorophyll b Chlorophyll a V B G YO R Photosynthesis requires light and chlorophyll, which absorbs light energy. In the graph, notice how chlorophyll a absorbs light in the violet and red regions of the visible spectrum, while chlorophyll b absorbs light in the blue and red regions of the visible spectrum. Formative Assessment: 1. Describe the process of photosynthesis, including the reactants and products. 2. Why are light and chlorophyll needed for photosynthesis? 3. Why are plants green? 4. How well would a plant grow under pure yellow light? Explain your answer. Warm-up: A look into the future It is 100 years in the future and you are a research scientist. An enormous volcanic eruption has recently sent huge quantities of dust and ash into the atmosphere. Working with a partner, make a list of how this event will affect each of the following: 1. photosynthesis 2. plant life 3. animal life 4. human societies The Reactions of Photosynthesis • Photosynthesis occurs in 2 stages. – Light dependent Reaction – Light –Independent Reaction (Dark Reaction) First Stage of Photosynthesis • Light-dependent Reactions = occurs in the thylakoid membranes of chloroplast. – Produces oxygen gas – ADP ATP – NADP+ NADPH (electron carrier molecule) • Play video clip Part 2 Light-Dependent Reactions Section 8-3 Photosystem II Hydrogen Ion Movement Chloroplast ATP synthase Inner Thylakoid Space Thylakoid Membrane Stroma Electron Photosystem I ATP Formation Transport Chain Chapter 8 Cellular Energy Light-independent Reactions (Calvin Cycle or Dark Reaction) • Occurs in the stroma – When sunlight excites electrons in chlorophyll, the electrons gain energy. – The electrons are carried by a certain electron-carrying molecule called NADP+ – NADP+ accepts and holds 2 excited electrons along with Hydrogen (H+). NADP+ is then converted into NADPH • • – NADP+ = oxidized form NADPH = Reduced form NADPH carries excited electrons from the light-dependent reactions and uses them to produce high-energy sugars like glucose. Play Video Clip Light Independent Reaction Calvin Cycle CO2 Enters the Cycle Energy Input ChloropIast 5-Carbon Molecules Regenerated 6-Carbon Sugar Produced Sugars and other compounds Chapter 8 Cellular Energy Photosynthesis: An Overview Section 8-3 Light CO2 Chloroplast Chloroplast NADP+ ADP + P LightDependent Reactions Calvin Cycle ATP NADPH O2 Sugars Concept Map Section 8-3 Photosynthesis includes Lightdependent reactions Calvin cycle use take place in Energy from sunlight Thylakoid membranes to produce ATP Go to Section: NADPH O2 takes place in Stroma uses ATP NADPH of to produce Chloroplasts High-energy sugars Factors Affecting the Rate of Photosynthesis: • Shortage of water: slows or stops photosynthesis – Name some plant adaptations that can reduces water loss • Temperature: to far above or below the temp. range can damage the enzymes that help photosynthesis to function. – At very low temps. - slow or stop photosynthesis – At very high temps. - slow or stop photosynthesis • Amount of light: – Increase light = increase photosynthesis – Decrease light = decrease photosynthesis See Interpreting Graphs Pop Quiz: 1. Summarize the light-dependent reactions. 2. What reactions make up the Calvin cycle? 3. What is the function of NADPH? 4. How is light energy converted into chemical energy during photosynthesis? 5. Can the complete process of photosynthesis take place in the dark? Explain your answer. 6. Oxygen is made during which reaction of photosynthesis (light dependent or light independent)? 7. Glucose is made during which reaction of photosynthesis (light dependent or light independent)? Ticket Out The Door • See handout for options • Homework: – Graphic Organizer Due Thursday – Chapter 8 Guided reading Due Friday Cellular Respiration Warm-up: • Do you like to run, bike, or swim? These all are good ways to exercise. When you exercise, your body uses oxygen to get energy from glucose, a six-carbon sugar. Go to Section: Warm-up: 1. How does your body feel at the start of exercise, such as a long, slow run? How do you feel 1 minute into the run; 10 minutes into the run? 2. What do you think is happening in your cells to cause the changes in how you feel? 3. Think about running as fast as you can for 100 meters. Could you keep up this pace for a much longer distance? Explain your answer. Mitochondrion Structure Living things get the energy they need from food. Both plant and animal cells carry out the final stages of cellular respiration in the mitochondria. Chemical Energy and Food • How much energy is actually present in food? – One gram of the sugar glucose when burned in the presence of oxygen, releases 3811 calories of heat energy. – A calorie is the amount of energy needed to raise the temperature of 1 gram of water 1 Celsius degree. – The Calorie (capital “C”) that is used on food labels is a kilocalorie, or 1000 calories. – Cells, of course, don’t “burn” glucose. Instead, they gradually release the energy from glucose and other food compounds. Cellular Respiration • What is cellular respiration? – The catabolic pathway in which organic molecules are broken down to release energy for the cell to do work. • (cell division, membrane transport, movement, repair, etc….) • Who does cellular respiration? All living organisms Chemical Reactions • Cellular Respiration: 6O2 + C6H12O6+ ADP + Pi 6CO2 + 6H2O + ATP (reactants) (products) • Photosynthesis: 6CO2 + 6H2O + ATP 6O2 + C6H12O6 + ADP + Pi • The reactants of one process are the products of the other How does cellular respiration take place? • 3 stages – Glycolysis – Krebs Cycle – Electron Transport Chain First Stage: Glycolysis • Glycolysis takes place in the cytoplasm – Glucose (a 6 Carbon molecule) is broken in half – 2 molecules of pyruvic acid (a three carbon chain) are formed Glycolysis Glucose 2 Pyruvic acid To the electron transport chain Glycolysis – During this process 2 ATP are used to activate the process of glycolysis – 4 ATP molecules are made. A net gain of 2 ATP are produced. (-2 +4)=2 – 2 NAD+ molecules gain an electron in the form of hydrogen (H) to produce 2 NADH molecules. – 2 pyruvic acid molecules are also made – Overall Chemical Equation for Glycolysis: C6H12O6 + 2 NAD+ + 2 ADP 2Pyruvic Acid+ 2NADH + 2ATP Oxygen or No Oxygen What happens after glycolysis depends on whether there is oxygen present in the cell or not Glycolysis Krebs Cycle Fermentation Anaerobic= No oxygen Aerobic= Oxygen needed Alcohol Fermentation Lactic Acid Fermentation Electron Transport Chain Produces 2 ATP Produces 2 ATP Produces 36 ATP Fermentation: • Releases energy in food molecules in the absence of oxygen. – Anaerobic = does not require oxygen – Regenerates NAD+ so glycolysis can continue to produce ATP Two Types of Fermentation: • Alcohol Fermentation: – Occurs in yeast & a few other microorganism – Important part of bread baking – Pyruvic acid + NADH ethyl alcohol + CO2 + NAD+ • Lactic Acid Fermentation: – Occurs in animals – Pyruvic acid + NADH lactic acid + NAD+ – Lactic Acid build up in your muscle cells during exercise causes sore What Keeps Glycolysis Going? • Without NAD+, the cell cannot keep Glycolysis going, and ATP production would stop. • Therefore NAD+ needs to be replenished. • Lactic Acid Fermentation takes place to replenish NAD+ so that Glycolysis can continue to make ATP for the cell Lactic Acid Fermentation Section 9-1 Glucose Go to Section: Pyruvic acid Lactic acid Cellular Respiration Pop Quiz: 1. 2. 3. 4. 5. Describe the process of cellular respiration. What are the 3 products of glycolysis? Name the two main types of fermentation. What is a calorie? A Calorie? How is the function of NAD+ similar to that of NADP+? 6. How are lactic acid fermentation and alcoholic fermentation similar? How are they different? Cellular Respiration: Occurs in all living organisms • 3 stages to cellular respiration 1. Glycolysis = occurs in the cytoplasm • One 6 carbon glucose is split into two 3-carbon molecules called pyruvic acid • Anaerobic conditions = Alcohol or Lactic Acid Fermentation • Aerobic conditions = Kreb’s Cycle 2. Kreb’s Cycle 3. Electron Transport Chain Cellular Respiration Glucose (C6H1206) + Oxygen (02) Glycolysis Krebs Cycle Electron Transport Chain Carbon Dioxide (CO2) + Water (H2O) Kreb’s Cycle: • In the presence of oxygen, pyruvic acid is broken down into carbon dioxide. • Occurs in Mitochondrial Matrix • See Video Clip Kreb’s Cycle: • 2 Pyruvic acid can be converted, in the presence of oxygen, into 2 acetyl-CoA, and carbon dioxide is given off. • Acetyl-CoA is then converted into Citric Acid (First compound formed during this cycle, hence the alternated name “Citric Acid Cycle” • Citric Acid (6 carbon) is broken down into a five-carbon molecule and then a 4-carbon molecule. More carbon molecules are released and ATP is produced. • Reduced electron carrying molecules are also produced (4 NADH and 1 FADH2). Chapter 8 Cellular Energy 8.3 Cellular Respiration The net yield from the Krebs cycle is: six CO2 molecules two ATP eight NADH two FADH2. Electron Carrying Molecules • Reduced electron carrying molecules are also produced (4 NADH and 1 FADH2). • • • • NAD+ = oxidized form NADH = reduced form FADH = oxidized form FADH2 = reduced form • NADH and FADH2 are moved to the electron transport chain to generate the bulk of ATP that the cell will use to do work. Electron Transport Chain: • In eukaryotes = occurs in inner membrane of the mitochondrion • In prokaryotes = occurs in the cell membrane (Why? No membrane bound organelles) • The electron transport chain uses the highenergy electrons from the Krebs cycle to convert ADP into ATP. • See Video Clips (2) Electron Transport Chain Electron Transport Hydrogen Ion Movement Channel Mitochondrion Intermembrane Space ATP synthase Inner Membrane Matrix ATP Production Electron Transport Chain: • A = Electrons are transported down the membrane proteins • B = Hydrogen ions (H+) build up in the inner membrane space making it positively charge and the other side negatively charged. • C = The charge difference (review voltage- potential channels) causes a protein called ATP Synthase to rotate. Each time ATP Synthase rotates, a Phosphate group (PO4-3) is added to ADP to form ATP. • The electrons that were transported down the membrane proteins combine with hydrogen ions, which are accepted by oxygen to form water. So why do we need oxygen during the Kreb’s Cycle and the ECT? • Oxygen is the final electron acceptor of the electron transport chain to make water. • Oxygen is essential for getting rid of low-energy electrons and hydrogen ions, which are the wastes of cellular respiration. The total Energy From Glycolysis, the Kreb’s Cycle and the Electron Transport is 36 APT How efficient is the process of cellular respiration? • The 36 ATP molecules the cell makes per one glucose represents about 38 percent of the total energy of glucose. • What happens to the remaining 62 percent? – It is released as heat, which is one of the reasons your body feels warmer after vigorous exercise, and you do not freeze in winter – Remember energy pyramids…. Checkpoint Questions: 1. What happens to pyruvic acid during the Krebs cycle? 2. How does the electron transport chain use the highenergy electrons from the Krebs cycle? 3. Why is cellular respiration considered to be much more efficient than glycolysis alone? 4. How many molecules of ATP are produced in the entire breakdown of glucose? 5. Compare photosynthesis and cellular respiration. How are they similar? How are they different?