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Transcript
Mutations
Changes to DNA
2009-2010
Mutations
• Changes to DNA are called mutations
– change the DNA
– changes the mRNA
– may change protein
– may change trait
DNA
TACGCACATTTACGTACG
mRNA
AUGCGUGUAAAUGCAUGC
protein
aa aa aa aa aa aa aa
trait
Types of mutations
• Changes to the letters (A,C,T,G bases) in the
DNA
– point mutation
• change to ONE letter (base) in the DNA
• may cause change to protein, may not
– frameshift mutation
• addition of a new letter (base) in the DNA sequence
• deletion of a letter (base) in the DNA
• both of these shift the DNA so it changes how the
codons are read
• big changes to protein!
Point Mutations
• One base change
– can change the meaning of the whole protein
THEFATCATANDTHEREDRATRAN
THEFATCARANDTHEREDRATRAN
OR
THEFATCATENDTHEREDRATRAN
Does this change
the sentence?
A LITTLE!
Point Mutations
• Missense mutation = changes amino acid
AUGCGUGUAUACGCAUGCGAGUGA
MetArgValTyrAlaCysGluStop
AUGCGUGUAUACGUAUGCGAGUGA
MetArgValTyrValCysGluStop
Does
this change
the protein?
DEPENDS…
Sickle Cell Disease
Symptoms
Caused by a
genetic defect
Anemia
Pain
Frequent infections
Delayed growth
Stroke
Pulmonary hypertension
Organ damage
Blindness
Jaundice
gallstones
Carried by 5% of
humans
Carried by up to
25% in some
regions of Africa
Life expectancy
42 in males 48 in females
Sickle cell anemia
• Hemoglobin protein in red blood cells
– strikes 1 out of 400 African Americans
– limits activity, painful & may die young
Normal
round cells
Misshapen
sickle cells
Only 1 out of
146 amino acids
Point Mutations
• Silent mutation = no change to protein
AUGCGUGUAUACGCAUGCGAGUGA
MetArgValTyrAlaCysGluStop
AUGCGUGUAUACGCUUGCGAGUGA
MetArgValTyrAlaCysGluStop
The code
Does
this has
change
the protein?
repeats
in it!
Why not?
Point Mutations
• Nonsense mutation = change to STOP
AUGCGUGUAUACGCAUGCGAGUGA
MetArgValTyrAlaCysGluStop
Really destroyed
that protein!
AUGCGUGUAUAAGCAUGCGAGUGA
MetArgValStop
Frameshift Mutations
• Add or delete one or more bases
– changes the meaning of the whole protein
THEFATCATANDTHEREDRATRAN
Delete
Add one!
one!
Does this change
the sentence?
A LOT!
THEFATCANTANDTHEREDRATRAN
OR
THEFATCAANDTHEREDRATRAN
Frameshift Mutations
• Addition = add one or more bases
AUGCGUGUAUACGCAUGCGAGUGA
MetArgValTyrAlaCysGluStop
AUGCGUGUAUACGUCAUGCGAGUGA
MetArgValTyrValMetArgValA
Does this change
the protein?
A LOT!
Frameshift Mutations
• Deletion = lose one or more bases
AUGCGUGUAUACGCAUGCGAGUGA
MetArgValTyrAlaCysGluStop
AUGCGUGUAUACGAUGCGAGUGA
MetArgValTyrAspAlaSerGA
Does this change
the protein?
A LOT!
Cystic fibrosis
• Broken salt channel in cells
– strikes 1 in 2500 white births
– gene codes for a protein channel
that allows salt to flow across cell membrane
• broken protein doesn’t work as channel
• doesn’t allow salt out of cell, so water doesn’t flow out
either
• thicker & stickier mucus coating around cells
• mucus build-ups in lungs & causes bacterial infections
• destroys lung function
– without treatment children die before 5;
with treatment can live past their late 20s
Salt channel
transports salt through protein
Effect on Lungs
channel out of cell
normal lungs
airway
salt channel
salt
normal mucus
H 2O
cells lining
lungs
cystic fibrosis
salt
H 2O
Osmosis problems!

thick mucus
mucus & bacteria build up
= lung infections & damage
Deletion leads to Cystic
fibrosis
deletion
Loss of one
amino acid!