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FACS Flourescens Activeted Cell sortering användningsområden FACS diagram FACS modell Hydrodynamisk fokusering laminärntflöde Tubulent flöde Laminärtflöde parametrar Skum Foam The relatively stable foam of beer, for example, results from an interaction between a protein fraction rich in basic and aromatic amino acids and the hop bitter compounds. Developing and improving knowledge of the relationship between peptide concentration and structure and foam performance can lead to improved understanding, analysis and control of this complicated property of food products. Dr. Karl J. Siebert - (Tel: 315-787-2299) Email: [email protected] Ljusspridning - partikelstorlek Scattering Forward scatter Side scatter http://www.epa.gov/owow/volunteer/proceedings/sixth/session1.html Side scatter vs Forward scatter University of Utah scatterplot fluorimeteroptik Vaccine Research Center / 40 Convent Drive / Bethesda, Maryland 20892 Fluorimeter Flourescens färger Fluorescein Fluorescens plott Lin och log Sample : peripheral blood after red cell lysis. CD14 PE (log) Side Scatter (linear) Data collected on 10,000 cells. CD45 FITC (log) Forward Scatter (linear) R1=monocytes (CD14+ve) R2=lymphocytes (CD45>monocytes) R3=granulocytes (CD45<monocytes) [email protected] Isometric Displays Sample : peripheral blood after red cell lysis. Data collected on 10,000 Events High Events CD14 PE Low CD 45 FITC Scatter Fluorescence [email protected] Apoptotiska celler As cells die or become apoptotic the refractive index of the internal cytoplasm becomes more similar to that of the extracellular medium - this manifests itself as a reduction in forward scatter signal. At the same time, intracellular changes and invagination of the cytoplasmic membrane lead to an increase in side (or orthogonal or 90°) scatter. If we add a dead cell discriminatory dye we can identify cells that have become permeable. In this way we can get low level resolution of dead and apoptotic cells. science.cancerresearchuk.org/.../images/51998 endotelceller R2= total cells R1= living cells Isolation of lymph endothelial cells (LEC) from dermal skin capillary ECs a case study with cells from one donor pre-selected by CD31 magneto bead sorting www.reliatech.de
