Download Lab-Lecture8

Central Dogma
(for your disease?)
LB 145
February 22-23, 2011
Deoxyribonucleic Acid
Protein
(short protein called peptide)
• Protein =Amino Acids stuck together
• By what kind of bond?
• What kind of reaction is this?
From Gene to Peptide
Functional Peptide:
PROTEIN
DNA
1 Synthesis of
mRNA in the
nucleus
mRNA
NUCLEUS
CYTOPLASM
DNA
1 Synthesis of
mRNA in the
nucleus
mRNA
NUCLEUS
CYTOPLASM
mRNA
2 Movement of
mRNA into cytoplasm
via nuclear pore
DNA
1 Synthesis of
mRNA in the
nucleus
mRNA
NUCLEUS
CYTOPLASM
mRNA
2 Movement of
mRNA into cytoplasm
via nuclear pore
Ribosome
3 Synthesis
of protein
Polypeptide
Amino
acids
Cystic Fibrosis is most often caused by
mutation DF508 in the CFTR gene
o The CFTR gene is 189,000 bases long
There are 27 exons/ 26 introns in it.
o The mRNA (all exons) is 6129 bases
How many codons is that, max?
o The protein is 1480 amino acids long
Normally it’s a Cl- channel, the mutation?
What do you know of your
disease’s Central Dogma?
o What is the size of your gene?
How many exons/introns does it have?
o What is the size of your mRNA (cDNA)?
How many codons does it have?
o What is the size of your protein?
What is the normal vs abnormal function of it?
Reminders
• Peer Review DUE
– Might want to look over the criticisms soon-ish
• Order your primers by 5pm FRIDAY
• Check ATCC.org for cells and DNA.